EBI Dbfetch

ID   M31308; SV 1; linear; genomic DNA; STD; PRO; 2339 BP.
XX
AC   M31308;
XX
DT   22-NOV-1990 (Rel. 25, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   E.chrysanthemi exo-poly-alpha-D-galacturonosidase (pehX) gene, complete
DE   cds.
XX
KW   exo-poly-alpha-D-galacturonosidase.
XX
OS   Erwinia chrysanthemi
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Dickeya.
XX
RN   [1]
RP   1-2339
RX   PUBMED; 2168372.
RA   He S.Y., Collmer A.;
RT   "Molecular cloning, nucleotide sequence, and marker exchange mutagenesis of
RT   the exo-poly-alpha-D-galacturonosidase-encoding pehX gene of Erwinia
RT   chrysanthemi EC16";
RL   J. Bacteriol. 172(9):4988-4995(1990).
XX
CC   Draft entry and computer-readable sequence for [Unpublished (1990)
CC   Cornell Univ., Ithaca, NY 14853] kindly submitted
CC   by S.He, 16-JAN-1990.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2339
FT                   /organism="Erwinia chrysanthemi"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:556"
FT   mRNA            93..>2339
FT                   /gene="pehX"
FT                   /product="exo-poly-alpha-D-galacturonosidase"
FT   misc_signal     149..177
FT                   /gene="pehX"
FT                   /note="promoter"
FT   RBS             204..207
FT                   /gene="pehX"
FT                   /note="ribosome binding site"
FT   CDS             214..2022
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="pehX"
FT                   /product="exo-poly-alpha-D-galacturonosidase"
FT                   /EC_number="3.2.1.82"
FT                   /db_xref="GOA:P15922"
FT                   /db_xref="InterPro:IPR012334"
FT                   /db_xref="UniProtKB/Swiss-Prot:P15922"
FT                   /protein_id="AAA24842.1"
FT                   /translation="MKVITFSRRSALASIVATCLMSTPALAATAQAPQKLQIPTLSYDD
FT                   HSVMLVWDTPEDTSNITDYQIYQNGQLIGLASQNNDKNSPAKPYISAFYKSDAANFHHR
FT                   IVLQNAKVDGLKAGTDYQFTVRTVYADGTTSNDSNTVTTTTTAVPKVINITQYGAKGDG
FT                   TTLNTSAIQKAIDACPTGCRIDVPAGVFKTGALWLKSDMTLNLLQGATLLGSDNAADYP
FT                   DAYKIYSYVSQVRPASLLNAIDKNSSAVGTFKNIRIVGKGIIDGNGWKRSADAKDELGN
FT                   TLPQYVKSDNSKVSKDGILAKNQVAAAVATGMDTKTAYSQRRSSLVTLRGVQNAYIADV
FT                   TIRNPANHGIMFLESENVVENSVIHQTFNANNGDGVEFGNSQNIMVFNSVFDTGDDSIN
FT                   FAAGMGQDAQKQEPSQNAWLFNNFFRHGHGAVVLGSHTGAGIVDVLAENNVITQNDVGL
FT                   RAKSAPAIGGGAHGIVFRNSAMKNLAKQAVIVTLSYADNNGTIDYTPAKVPARFYDFTV
FT                   KNVTVQDSTGSNPAIEITGDSSKDIWHSQFIFSNMKLSGVSPTSISDLSDSQFNNLTFS
FT                   NLRSGSSPWKFGTVKNVTVDGKTVTP"
FT   misc_signal     2037..2058
FT                   /gene="pehX"
FT                   /note="terminator (put.); putative"
XX
SQ   Sequence 2339 BP; 619 A; 676 C; 583 G; 461 T; 0 other;
     cgacgccaac ggccatctta accgcaagaa ggggtaacaa tcggttaggg gtaacaattg        60
     gttacccctt tcttttatcc cctcgattga tttgtgatcc cttccgggga aaaaagaaat       120
     acgggatgta tttttataaa atagtatttt gaaaaaaaca tcccacacgg ataaaacacc       180
     gccatcaatt taaaagagaa ctaaggaaca aggatgaaag tcatcacatt ttcacgtcgt       240
     tcggcccttg cctcgatagt agccacatgc ctgatgagta caccagcact ggcggcaacg       300
     gctcaggcac cgcaaaaact ccagattccc acactgtctt acgacgacca tagcgtcatg       360
     ctggtgtggg acacgccgga agacacgtcc aacattaccg actatcagat ttaccaaaat       420
     ggccagttga ttggactcgc cagccaaaat aatgacaaaa actcgccggc caaaccgtac       480
     atcagtgcgt tctacaaaag tgacgccgcg aattttcacc accgaattgt cctgcaaaac       540
     gctaaagtcg atggtctgaa agccggcacc gattatcagt ttaccgttcg tacagtctat       600
     gccgatggca caacttcgaa cgacagtaac acagttacca ccaccaccac cgctgtaccc       660
     aaagtcatca acatcaccca gtacggcgcc aaaggcgacg gcactacgct caataccagc       720
     gcgattcaga aagcgattga cgcctgccca accggttgcc gtatcgacgt tccggccggg       780
     gtgttcaaaa ccggcgctct gtggctgaaa agcgatatga cgctgaatct gttgcaaggt       840
     gcaacgctgc tggggtcgga taatgcggcc gactatcccg atgcgtataa aatttatagc       900
     tacgtctcgc aggtacgccc tgcttcactg ctgaacgcta tcgataaaaa cagctcggct       960
     gtgggcacct ttaaaaacat ccgtatcgtc ggtaaaggca ttatcgacgg caacggctgg      1020
     aaacgcagtg cggatgccaa agatgaactg ggcaataccc tgccgcagta cgtgaagagt      1080
     gataacagca aagtgagcaa agatggcatt ctggcgaaaa accaggttgc ggcggccgtg      1140
     gctaccggca tggataccaa aaccgcctac agccagcgcc gctccagttt ggtcacccta      1200
     cgcggcgtcc aaaacgctta tatcgccgat gtcaccatcc gcaacccagc taaccacggc      1260
     atcatgttcc tggaaagcga gaatgtggtg gaaaacagcg ttatccacca gacgttcaat      1320
     gccaacaatg gcgatggcgt cgaatttggc aacagccaaa acatcatggt attcaacagc      1380
     gtgttcgata ccggggacga tagcatcaac ttcgcagcag ggatgggcca ggacgcacag      1440
     aagcaagagc cttcgcaaaa cgcctggttg ttcaacaact ttttccgtca cggccacggc      1500
     gctgtggtgt taggcagcca caccggcgca gggattgtcg atgttctggc ggaaaacaac      1560
     gtgataaccc agaacgatgt gggtctgcgc gccaagagcg ccccggccat cggcggcggc      1620
     gcacatggca tcgtgttccg caacagcgcg atgaaaaacc tggccaaaca ggccgtcatc      1680
     gtgacgttaa gttacgccga caacaacggc accatcgact ataccccggc caaagtgccc      1740
     gcacgattct atgacttcac cgtcaaaaac gtcaccgtgc aggacagtac cggctcaaac      1800
     ccggcgattg agatcaccgg cgacagcagc aaagatatct ggcatagcca gtttatcttc      1860
     agcaacatga aactcagcgg cgtaagcccg acgtcaatca gcgacctgag cgacagccag      1920
     ttcaacaacc tgacgttcag caatctgcgc agcggctctt caccgtggaa attcggtacc      1980
     gtgaaaaacg tgaccgtaga cggtaaaacc gtcaccccct gacggtaagc ctcaacacgc      2040
     cgggccatgg cgcccgcggt atccgcttcc cgtgttagcc tttgtctctg gccttcgcca      2100
     attgagcgcg taggttcgcc agatggttct gccctttctg catcgctctg cgggctgacg      2160
     actttgcgtt cggtttccca catcacatca tctgcggcag ttccagcaaa aaacgactgg      2220
     gctccgggcg gatcaactcg ccgtattcgc ggctcgcggc acagggtgaa cgttaactct      2280
     ttctgagccc gggtgatacc gacgtaggcc agacggcgct cctcgtcgac ggtcccgat       2339
//