EBI Dbfetch

ID   M29849; SV 1; linear; genomic DNA; STD; PRO; 1289 BP.
XX
AC   M29849;
XX
DT   28-JUN-1990 (Rel. 24, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   E.coli raffinose repressor (rafR) gene, complete cds, and
DE   alpha-galactosidase (rafA) gene, 5' end.
XX
KW   alpha-galactosidase; raffinose repressor.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RP   1-1289
RX   PUBMED; 2180920.
RA   Aslanidis C., Schmitt R.;
RT   "Regulatory elements of the raffinose operon: nucleotide sequences of
RT   operator and repressor genes";
RL   J. Bacteriol. 172(4):2178-2180(1990).
XX
CC   Draft entry and computer-readable sequence for [1] kindly submitted
CC   by R.Schmitt, 13-NOV-1989.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1289
FT                   /organism="Escherichia coli"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:562"
FT   -35_signal      120..126
FT   -10_signal      144..149
FT   RBS             156..161
FT                   /note="ribosome binding site"
FT   CDS             166..1176
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="raffinose repressor"
FT                   /db_xref="GOA:P21867"
FT                   /db_xref="HSSP:1LCC"
FT                   /db_xref="InterPro:IPR001761"
FT                   /db_xref="UniProtKB/Swiss-Prot:P21867"
FT                   /protein_id="AAA24500.1"
FT                   /translation="MSLKAIATTLGISVTTVSRALGGFSDVAASTRERVEAEARRRGYR
FT                   PNTQARRLKTGKTDAIGLVYPENDVPFNSGVFMDMVSCISRELAYHDIDLLLIADDEHA
FT                   DCHSYMRLVESRRIDALIIAHTLDDDPRITHLHKAGIPFLALGRVPQGLPCAWFDFDNH
FT                   AGTWQATQKLIALGHKSIALLSENTSHSYVIARRQGWLDALHEHGLKDPLLRLVSPTRR
FT                   AGYLAVMELMSLPAPPTAIITDNDLSGDGAAMALQLRGRLSGKEAVSLVVYDGLPQDSI
FT                   IELDVAAVIQSTRSLVGRQISDMVYQIINGASPESLQITWTPIFYPGSTVHSPSF"
FT   misc_feature    1196..1213
FT                   /note="raf operator"
FT   -35_signal      1212..1216
FT   misc_feature    1217..1234
FT                   /note="raf operator"
FT   -10_signal      1235..1240
FT   RBS             1258..1263
FT                   /note="ribosome binding site"
FT   CDS             1270..>1289
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="alpha-galactosidase"
FT                   /db_xref="GOA:P16551"
FT                   /db_xref="InterPro:IPR000111"
FT                   /db_xref="UniProtKB/Swiss-Prot:P16551"
FT                   /protein_id="AAA24501.1"
FT                   /translation="MISKYC"
XX
SQ   Sequence 1289 BP; 317 A; 283 C; 326 G; 363 T; 0 other;
     aagcttatta tgcttccatc ggaaacaatg atctggcagc ctgagttcac agataaaata        60
     tctctccagg aaacccgggg ctgttcatca tgcaagtctg tcgattactg gctttggtga       120
     cggaattttc tggatttccg gcttagaacc acagcaggag ataatatgtc acttaaagcg       180
     attgccacga cactcggtat ttctgtcacc actgtcagtc gggctcttgg aggcttttca       240
     gatgtggctg cttctacccg tgagcgcgtg gaagcggaag cacgtcgacg aggttaccgc       300
     cctaatacac aggcaagaag actcaaaacc ggtaaaaccg atgctatcgg tctggtttat       360
     cctgaaaatg atgtgccgtt taacagcggt gtttttatgg atatggtcag ttgcatcagc       420
     agggaacttg cttatcatga tattgactta ctgctgatcg ctgatgatga gcatgcagac       480
     tgccacagct atatgcggct tgttgaaagt cgcagaattg atgctcttat cattgcacat       540
     actctggatg acgatccccg tatcacacat cttcataaag caggtattcc gtttctggct       600
     cttggacggg taccgcaggg cttgccctgt gcgtggtttg actttgataa tcatgccgga       660
     acctggcagg caacccagaa gctgattgct ttgggacata agagtattgc gctgttgagc       720
     gagaacactt cacattctta tgttattgca agacgtcagg gatggcttga tgcactgcat       780
     gagcatggac tgaaagatcc attgttgcgg ctggtttctc ccacgcgacg agcgggctat       840
     ctggctgtga tggagttaat gtcattaccg gcgccaccaa cagctattat tactgacaat       900
     gacctgagtg gagatggtgc ggctatggcg ctgcagttga gagggcgtct ttcagggaaa       960
     gaagctgtat ctctggttgt atatgatggt ttgcctcagg acagcattat tgagctggat      1020
     gtggctgctg ttattcagtc aacacgaagt ctcgttggtc gtcagatttc tgacatggtg      1080
     tatcagataa tcaatggtgc atcaccagaa tcactgcaga taacctggac accgatattt      1140
     taccctggta gcacggttca ttctccttcc ttctgatttt ttatccagat cacacaaccg      1200
     aaacgttttg gttgatgttc gaaacgtttc ggatcaacag taagacatac ctgaaagcgg      1260
     agatgtctaa tgatttcaaa gtactgcag                                        1289
//