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EBI DbfetchID M29292; SV 1; linear; genomic DNA; STD; PRO; 4382 BP. XX AC M29292; XX DT 30-JUN-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Bacillus sphaericus IF03525 bioDAYB operon encoding dethiobiotin synthase DE (bioD), adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA), DE biotin synthase (bioY) and bioB genes, complete cds. XX KW adenosylmethionine-8-amino-7-oxononanoate aminotransferase; bioB; KW biotin synthase; dethiobiotin synthase. XX OS Lysinibacillus sphaericus OC Bacteria; Firmicutes; Bacillales; Planococcaceae; Lysinibacillus. XX RN [1] RP 1-4382 RA Gloeckler R., Ohsawa I., Speck D., Ledoux C., Bernard S., Zinsius M., RA Villeval D., Kisou T., Komogawa K., Lemoine U.; RT ; RL Unpublished. XX RN [2] RP 456-511 RX DOI; 10.1016/0378-1119(90)90496-E RX PUBMED; 2110099. RA Gloeckler R., Ohsawa I., Speck D., Ledoux C., Bernard S., Zinsius M., RA Villeval D., Kisou T., Kamogawa K., Lemoine Y.; RT "Cloning and characterization of the Bacillus sphaericus genes controlling RT the bioconversion of pimelate into dethiobiotin"; RL Gene 87(1):63-70(1990). XX CC Draft entry and computer-readable sequence for [2] kindly submitted CC by R.Goleckler, 20-OCT-1989, for release after publication. XX FH Key Location/Qualifiers FH FT source 1..4382 FT /organism="Lysinibacillus sphaericus" FT /strain="IF03525" FT /mol_type="genomic DNA" FT /db_xref="taxon:1421" FT mRNA <456..4364 FT /note="bioDAYB mRNA" FT RBS 491..503 FT /note="ribosome binding site" FT gene 509..1213 FT /gene="bioD" FT CDS 509..1213 FT /codon_start=1 FT /transl_table=11 FT /gene="bioD" FT /product="dethiobiotin synthase" FT /db_xref="GOA:P22818" FT /db_xref="HSSP:1DTS" FT /db_xref="InterPro:IPR004472" FT /db_xref="UniProtKB/Swiss-Prot:P22818" FT /protein_id="AAB02324.1" FT /translation="MQHFWVVGTDTDVGKTFVTTLLMRNLQKQGVRVTPYKPVQTGEVY FT DGEQAYYFDTAMYEKYSLQLLDRENLNGYSFKEAASPHFAAQLEGQQIDTQQLLKQMQL FT LQQTWDVVICEGAGGLFVPLDACGETTLLDVIVESKLPVVVVTRTALGTINHTLLTLEA FT LTTRKIEVLGLVFNGDMGSRMEQDNIQTILQYYTLPYMTIPKLEELSDINEYAITGTSL FT FERLIRRETSIN" FT RBS 1179..1186 FT /gene="bioD" FT /note="ribosome binding site" FT gene 1194..2561 FT /gene="bioA" FT CDS 1194..2561 FT /codon_start=1 FT /transl_table=11 FT /gene="bioA" FT /product="adenosylmethionine-8-amino-7-oxononanoate FT aminotransferase" FT /db_xref="GOA:P22805" FT /db_xref="HSSP:1QJ3" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:P22805" FT /protein_id="AAB02325.1" FT /translation="MKQVLTELQEKDLQHVWHPCSQMKDYEAFPPIVIKKGEGVWLYDE FT QNQRYLDAVSSWWVNLFGHANPRISQALSEQAFTLEHTIFANFSHEPAIKLAQKLVALT FT PQSLQKVFFADNGSSAIEVALKMSFQYHMQTGKTQKKRFLALTDAYHGETLGALSVGGV FT DLYNEVYQPLLLDTVRAQGPDCFRCPFKHHPDSCHAQCISFVEDQLRMHHKEITAVIIE FT PLIQAAAGMKMYPAIYLRRLRELCTQYDVHLIADEIAVGFGRTGTLFACEQANISPDFM FT CLSKGLTGGYLPLSVVMTTNDVYQAFYDDYATMKAFLHSHSYTGNTLACRVALEVLAIF FT EEEQYIDVVQDKGERMRKLALEAFSDLPFVGEYRQVGFVGAIELVANRDTKEPLPSEER FT IGYQIYKRALAKGLLIRPLGNVLYFMPPYIITDDEMQFMIQTTKDTIVQFFEEREG" FT RBS 2545..2556 FT /gene="bioA" FT /note="ribosome binding site" FT gene 2566..3156 FT /gene="bioY" FT CDS 2566..3156 FT /codon_start=1 FT /transl_table=11 FT /gene="bioY" FT /product="biotin synthase" FT /db_xref="GOA:P22819" FT /db_xref="InterPro:IPR003784" FT /db_xref="UniProtKB/Swiss-Prot:P22819" FT /protein_id="AAB02326.1" FT /translation="MLKQQSTLSLVMIAMFAALTAVGAFIKIPLPLVPFTLQIVFVFLA FT GCLLGGRNGFQSQLVYIGIGLVGLPVFTQGGGITYVLQPTFGYLIGFALAALVIGYMID FT RVESPTKKHFIVANIIGLIIIYAVAVPYLYVALNVWLNMKSSWSHVFLVGFVNSIVADF FT CLAIASALLAERLYKVFRSARAIKLVQIEKENV" FT gene 3156..4154 FT /gene="bioB" FT CDS 3156..4154 FT /codon_start=1 FT /transl_table=11 FT /gene="bioB" FT /db_xref="GOA:P19206" FT /db_xref="InterPro:IPR007197" FT /db_xref="UniProtKB/Swiss-Prot:P19206" FT /protein_id="AAB02327.1" FT /translation="MNWLQLADEVIAGKVISDDEALAILNSDDDDILKLMDGAFAIRKH FT YYGKKVKLNMIMNAKSGYCPEDCGYCSQSSKSTAPIEKYPFITKEEILAGAKRAFENKI FT GTYCIVASGRGPTRKDVNVVSEAVEEIKAKYGLKVCACLGLLKEEQAQQLKEAGVDRYN FT HNLNTSERHHSYITTTHTYEDRVNTVEVVKKHGISPCSGAIIGMKETKMDVVEIARALH FT QLDADSIPVNFLHAIDGTKLEGTQDLNPRYCLKVLALFRYMNPSKEIRISGGREVNLGF FT LQPFGLYAANSIFVGDYLTTEGQEANSDYRMLEDLGFEIELTQKQEEAFCS" FT RBS 4317..4364 FT /note="ribosome binding site" XX SQ Sequence 4382 BP; 1376 A; 746 C; 952 G; 1308 T; 0 other; aagctttgca cacttctgtt tcgtatcctc atattgaact tgatgaaacc ttcctatggc 60 cgtatgcatt gagatttttt ctcgatgttc tgcttgcaat gttcgatatt cttcttgccg 120 aatagctaca cgataccaaa attcataacg caacggtaaa tctcttattt cgtaagtaag 180 caaagtattt aaaatactgc tcatttgttc atatgtatct agctttttat ctgtctcctt 240 aaatagtcca aacattttgc caccccctgt tttgattaat actacaacct atgataaaaa 300 ccctttaata tttcttggga aataatccaa cgttgataaa acggggtgaa tatccgatca 360 atcgagtgaa atttaggata gaataccctc ggaaaaagca ttatctgaat catttatgta 420 aaaatgcaaa aaaaggcatt tacaaaagga aaaagaatgt gttaacttaa aaactatagt 480 tggttaacta aaagaggggg aggtacagtt gcaacacttt tgggttgttg gaacagatac 540 agatgttgga aaaacatttg tcaccacatt attaatgcgt aatttgcaaa aacagggcgt 600 acgtgtaacg ccttataaac cagtccaaac tggtgaagtg tatgatggtg aacaagccta 660 ttacttcgac acagcgatgt atgaaaaata ttccttgcaa ttgctagaca gagagaattt 720 aaatggctat tcatttaaag aggctgcatc gccacatttt gcggctcaac tggaggggca 780 gcaaattgac acacagcagt tattaaagca aatgcaactt ttacagcaaa catgggatgt 840 tgttatttgt gaaggagcgg gtgggctctt tgtgccatta gatgcatgtg gcgaaacgac 900 attgttggat gtcattgttg aaagtaaact acccgttgtc gtggttacac gaacagcact 960 aggaacaatt aaccatacgc tcttaacgtt agaggcattg actacacgga aaattgaagt 1020 gcttggtctt gtatttaacg gtgatatggg gagcaggatg gagcaagaca atatccaaac 1080 gattttacag tattatacat tgccctatat gacgatacca aagctggaag agctgtcgga 1140 cattaatgag tatgcaatta cgggcacatc attgtttgaa aggctgatta gacgtgaaac 1200 aagtattaac tgagctacaa gaaaaagatt tacaacatgt ctggcatcct tgctcacaaa 1260 tgaaagatta tgaggctttt ccaccaatcg ttataaaaaa aggcgaaggt gtatggctgt 1320 atgatgaaca gaatcaacgc tatcttgatg cggtatcttc atggtgggtc aatttatttg 1380 gacatgccaa tccacgtatt agccaagcat taagtgaaca agcatttacg ttggagcata 1440 caatttttgc gaatttttca catgagccag cgattaaact cgcacaaaaa ttagtagctt 1500 taacaccaca aagtttacaa aaagtatttt ttgcagataa tggttcatct gctatagaag 1560 tcgctttaaa aatgagtttt caatatcata tgcaaacggg gaaaacgcaa aaaaaacgct 1620 ttttggcatt aacggatgcc taccatggtg aaacattagg tgctttatcc gtcggtggcg 1680 tagatcttta taacgaagtg tatcaaccac tgttattgga tacggtacga gcacaaggcc 1740 cagattgttt ccgttgccca ttcaagcatc atccggatag ttgccatgcc caatgtatta 1800 gttttgtaga ggatcagttg cgcatgcatc ataaggaaat tacggcggtt attattgagc 1860 cactcattca agcggcagca gggatgaaaa tgtatccagc tatttatttg cgacgtttac 1920 gtgaactatg tacgcaatat gatgtgcatc taattgcaga cgaaattgct gtaggttttg 1980 ggcgcacagg tacacttttt gcctgtgagc aggctaatat ctctccggat tttatgtgtt 2040 tatcaaaagg tttaacaggt gggtatttac cactgtctgt cgtaatgacg acgaatgatg 2100 tatatcaggc attttatgat gattatgcca cgatgaaggc gtttttacat tcacatagtt 2160 acacagggaa tacacttgcc tgccgtgttg ctctagaggt attggcgata tttgaagaag 2220 aacagtatat agacgttgtg caagacaaag gtgaacgcat gcgaaagcta gccttggagg 2280 cttttagtga tttacctttt gttggtgaat atcggcaagt tgggtttgtc ggggcgattg 2340 aacttgtggc gaatcgcgat accaaagagc cattaccgag tgaggagcgc atcggctatc 2400 aaatatacaa aagagcttta gcaaaagggt tactgattcg tccacttggg aatgttttgt 2460 atttcatgcc accatacatt ataacggacg atgaaatgca atttatgatt caaacaacaa 2520 aagatacaat tgttcaattt tttgaagagc gggagggatg agggcatgtt gaaacaacag 2580 tcaacgttat cacttgtgat gattgcgatg tttgctgcat taacagcagt tggtgccttc 2640 attaaaattc cattaccgct cgtgccgttt acattacaaa ttgtctttgt ctttttagcg 2700 ggttgcttac tcggtggtcg caatggattt caaagtcagc tagtttacat aggaataggt 2760 ttagttggct tgccagtttt tacacaaggt ggaggcatta catatgtatt gcagccgact 2820 tttggttact taataggatt tgctcttgct gcattagtaa tcggctatat gattgatcga 2880 gtagaatcac caacgaaaaa gcatttcatt gttgccaata ttatagggct tatcattatt 2940 tatgcagtcg cagtacctta tttatatgta gcattaaatg tatggttaaa catgaaatca 3000 agttggtctc atgtattttt agtaggcttt gtcaatagta ttgttgcaga cttttgctta 3060 gcaattgctt ctgccctttt agctgaacgt ctatacaaag tattccgttc cgctagagct 3120 ataaaacttg tgcaaattga aaaggagaat gtttagtgaa ttggttacaa ttagcagatg 3180 aagtgattgc aggcaaggta attagcgatg atgaggcact tgccatttta aatagtgatg 3240 atgatgatat tttaaagcta atggacggcg catttgccat tcgtaagcac tattacggta 3300 aaaaagtaaa gttaaatatg attatgaatg ctaaaagtgg ctattgccca gaggattgtg 3360 gctattgctc gcagtcatct aaatcgaccg ctcctattga gaaatatccg ttcattacaa 3420 aagaagaaat attagcgggg gcaaagcgtg cgtttgaaaa taaaattggt acgtattgca 3480 tcgtcgcaag cggacgtggg ccgactcgta aagatgtcaa tgtagtgagt gaagccgttg 3540 aagaaattaa agcaaaatat ggcttaaaag tttgcgcttg cttaggttta ctaaaagaag 3600 aacaagcaca acaattaaaa gaagcgggtg ttgatcgcta caatcataac ttaaatacat 3660 cagagcgtca ccattcctat attacgacga cgcacacata tgaggatcgt gttaataccg 3720 ttgaggttgt aaagaaacat ggtatttccc catgttctgg agccattatt gggatgaaag 3780 aaacgaaaat ggatgtcgtg gaaattgcac gcgcattgca tcagttggac gcggattcaa 3840 ttccagttaa cttcttacat gcaattgatg gaacgaaact tgaaggaaca caggacttaa 3900 atcctcgcta ttgcttaaaa gtattagcgt tattccgcta catgaatcct tcgaaggaaa 3960 ttagaatttc cggtggtcgc gaagtcaatt taggattcct tcagccattt ggactgtatg 4020 cagcaaatag tatttttgtt ggggattact taactactga aggacaagaa gccaatagcg 4080 attatcgtat gcttgaagat ttgggctttg aaatcgagct gacacaaaag caagaagaag 4140 cattttgttc ttaattcaac caatcattat gaaataaaat ctactactac acaatatgat 4200 tacctcaaaa ccgtgtgagc gtcgtggaaa aggcgcacag acggtttttt ggtcgataaa 4260 agagaaggag aaaggtaaat aaatggttcc gataatatac ctataaaatg atggttttca 4320 caaaatgttc aatgaaagcg ttttgaaatt gaacagtttg tgaagggctt cacataaagc 4380 tt 4382 // |
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