 |
|
EBI DbfetchID M27273; SV 1; linear; genomic DNA; STD; PRO; 5284 BP. XX AC M27273; XX DT 01-FEB-1990 (Rel. 22, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE E.coli rafA, rafB, and rafD genes encoding alpha-D-galactosidase, DE raf-permease, and raf-invertase, complete cds. XX KW alpha-D-galactosidase; raf-invertase; raf-permease; rafA gene; rafB gene; KW rafD gene. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-5284 RX PUBMED; 2556373. RA Aslanidis C., Schmid K., Schmitt R.; RT "Nucleotide sequences and operon structure of plasmid-borne genes mediating RT uptake and utilization of raffinose in Escherichia coli"; RL J. Bacteriol. 171(12):6753-6763(1989). XX CC Draft entry and computer readable copy of sequence [1] kindly CC provided by R.Schmitt, 01-SEP-1989. XX FH Key Location/Qualifiers FH FT source 1..5284 FT /organism="Escherichia coli" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT -35_signal 12..17 FT -10_signal 36..41 FT RBS 58..63 FT /note="S.D. sequence (ribosome binding site)" FT CDS 70..2196 FT /codon_start=1 FT /transl_table=11 FT /note="alpha-D-galactosidase" FT /db_xref="GOA:P16551" FT /db_xref="InterPro:IPR000111" FT /db_xref="UniProtKB/Swiss-Prot:P16551" FT /protein_id="AAA24497.1" FT /translation="MISKYCRLSSPRSDLIIKTHPHAEIIWWGSALKHFSPDDCASLER FT PVANGRLDIDTPLTLIAENALGLFSSPGLEGHRNGLDASPVFYTVDVEHTENTLRLTSE FT DSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPVAERAEEVMAFHGRW FT TREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWSGNHR FT MRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYHRFLRD FT EIIRFPEQKLRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFKGRNDDR FT AALGDWYTDEQKYPNGLMPVINHVKSLGMEFGIWVEPEMINPDSDLFRLHPDWILSMPG FT YSQPTGRYQYVLNLNIPEAFDYIYKRFLWLLGEHPVDYVKWDMNRELVQAGHEGRAAAD FT AQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNNDALERCTIQR FT GMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVAADAKESDGYR FT RYALLYKEWRQLIHTGVLWRVDMPDSSIQVQGVVSPDQSQALFMISQLAMPDYTLPGIL FT RFPGLAAEVRYRLRVIDHPEIQLVGEGGHTMRRLPAWMNQPLEASGEWLAKGGIQLPVL FT DPESAILIALERAV" FT RBS 2244..2249 FT /note="S.D. sequence (ribosome binding site)" FT CDS 2259..3536 FT /codon_start=1 FT /transl_table=11 FT /note="raf-permease" FT /db_xref="GOA:P16552" FT /db_xref="HSSP:1PV6" FT /db_xref="InterPro:IPR018457" FT /db_xref="UniProtKB/Swiss-Prot:P16552" FT /protein_id="AAA24498.1" FT /translation="MNSASTHKNTDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLSK FT TDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLLHL FT NIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSSGFEYGKARMFGCLGWALCATMAGI FT LFNVDPSLVFWMGSGGALLLLLLLYLARPSTSQTAMVMNALGANSSLISTRMVFSLFRM FT RQMWMFVLYTIGVACVYDVFDQQFAIFFRSFFDTPQAGIKAFGFATTAGEICNAIIMFC FT TPWIINRIGAKNTLLVAGGIMTIRITGSAFATTMTEVVILKMLHALEVPFLLVGAFKYI FT TGVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIVLTVTVIS FT AFTLSSSPGIVHPSVEKAPVAHSEIN" FT RBS 3523..3528 FT /note="S.D. sequence (ribosome binding site)" FT CDS 3536..4966 FT /codon_start=1 FT /transl_table=11 FT /note="raf-invertase" FT /db_xref="GOA:P16553" FT /db_xref="InterPro:IPR006232" FT /db_xref="UniProtKB/Swiss-Prot:P16553" FT /protein_id="AAA24499.1" FT /translation="MKQRLSLAQSALEKLSARRGNTWYPIFHLAPPAGWMNDPNGLIYF FT NGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHELVALAPGDKYDRDGCFSGSAVDD FT DGVLSLIYTGHICLEDRGNDSIIREVQCLATSHDGIRFEKQGCVLTPPEGIMHFRDPKV FT WHEDGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGESYMWECPDFFRCG FT NFHWLMFSPQGMNPSGYRFRNLFQSGVLAGNWKPGSVFALKGVFEELDYGHDFYAPQSM FT LAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREVESLRRKC FT QPLSPVRLHGVQLLTENVQAAELLVTWHTVDSHAEHYGIRLGEGLRFYVDNQAGRLILW FT RYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYPQADSRQLSL FT YAAHGDAILTDGTLWMLT" XX SQ Sequence 5284 BP; 1199 A; 1166 C; 1406 G; 1513 T; 0 other; aaacgttttg gttgatgttc gaaacgtttc ggatcaacag taagacatac ctgaaagcgg 60 agatgtctta tgatttcaaa gtactgcaga ctgagcagtc ctcgctctga tttaattatt 120 aaaacccatc cgcatgcaga aattatctgg tggggctctg cactgaaaca tttctcaccg 180 gatgactgtg ccagcctgga aagaccagtt gcgaatggtc gtctggatat tgatacgcca 240 ctgacactga tcgctgaaaa tgcccttgga ctttttagtt ctccgggact ggaaggacac 300 agaaatggac tggatgcatc tcctgttttt tatacagttg acgtggaaca taccgaaaac 360 accctgagac ttaccagtga agattcggta gccggcctgc gtctggtcag cgagctggtg 420 atgacgccat cggggattct gaaagttcgt catgcactga ccaacctcag agagggagac 480 tggcagataa atcgtttcgc aatcacttta cctgtagctg aacgtgcgga agaagtcatg 540 gcctttcacg gacgctggac tcgtgaattt cagccgcaca gggtacgtct tactcatgat 600 gcttttgttc tggaaaatcg cagagggcgg acatctcatg agcattttcc ggcgctgatt 660 gtcggcacac caggcttctc ggaacaacag ggagaggtgt gggctgtgca tctggggtgg 720 agtggaaatc accgcatgag atgtgaggca aaaactgatg gcaggcgtta cgtacaggct 780 gaggctctgt ggatgccggg tgagaaggct ctcaggaaga atgaaaccct gtacaccccg 840 tggctatatg cctgccactc tgcggatggc ctgaatggaa tgagtcagca ataccatcgt 900 tttttgcgtg atgaaattat ccgtttccct gagcaaaaac tccgccccgt acatctcaat 960 acctgggaag gtatttattt caatcacaat cctgattaca tcatgcagat ggctgagcgt 1020 gcagcagcac tgggcgttga acgtttcatt attgatgatg gctggtttaa aggacgtaac 1080 gatgaccgcg cggctctggg cgactggtat accgatgaac agaaataccc gaacgggctg 1140 atgccggtta ttaatcatgt gaaatctctc ggtatggagt ttggtatatg ggttgaacca 1200 gaaatgatta atccggattc tgacctgttt cgtcttcatc cggactggat attgtcaatg 1260 ccaggatatt ctcagccaac cggaagatat cagtatgttc ttaacctgaa tattccggag 1320 gcctttgatt atatttataa acgtttctta tggttactgg gagaacatcc ggttgattat 1380 gtgaaatggg acatgaatcg tgagcttgta caggcagggc atgaaggccg tgcggcagca 1440 gatgcacaga cccgtcagtt ctatcgattg cttgatctcc tccgtgaacg ttttccacat 1500 gttgagtttg agtcctgtgc ttccggtggg gggcgtattg acttcgaagt cctgaaacgc 1560 acacaccggt tctgggcatc tgacaataat gatgccctgg agcgctgcac catacaacgt 1620 ggcatgagtt actttttccc tcctgaggtg atgggggcac atattggcca tcgccgctgc 1680 catgcaactt tccggcagca cagcatcgct tttcgtgggc tgacggcatt gttcggccat 1740 atggggctgg agctggatcc ggtagccgca gatgcgaagg aatctgatgg ttatcgccgg 1800 tatgccttgc tctataaaga atggcgacaa ctgattcata caggtgttct ctggcgtgtg 1860 gatatgccgg attcttcgat acaggttcag ggagtcgtca gccctgatca gtctcaggct 1920 ctttttatga tcagccagct tgcaatgccg gattacacct taccaggcat acttcgtttt 1980 cccggactgg cggcagaagt gcgttaccgg cttcgggtta ttgatcaccc ggaaattcaa 2040 ctggttggtg aaggtggtca taccatgcgc agattaccag cctggatgaa ccagcccctc 2100 gaggccagtg gtgaatggct ggcaaaggga gggattcagc tccccgtact ggatcctgag 2160 agcgcgattt tgatagcact tgaaagagct gtgtgaagag agatacggag cctcagctcc 2220 gtcctgtttc tcaatattat cccgtgaggg taagaataat gaattctgca agtacgcata 2280 aaaatactga tttctggatt ttcggtctgt tcttttttct ctactttttc atcatggcaa 2340 cctgttttcc gtttttgcct gtatggctgt ccgatgtggt cggactgagt aaaacggata 2400 caggtatagt cttttcatgt ctgtctctgt ttgccatcag tttccagcca ttgcttggag 2460 tcatatcaga tcgcctggga ctgaaaaaaa acctgatctg gagtatcagc ctgttactgg 2520 tatttttcgc cccctttttt ttatatgtat ttgcgcccct gttgcacctc aatatctggg 2580 caggtgcact gactggcggt gtctttattg gttttgtttt ttctgcaggt gccggagcta 2640 ttgaagctta tatagagcgg gtcagtcgca gtagtggatt tgaatacggt aaagcgagga 2700 tgttcgggtg tctgggttgg gcgttatgtg caactatggc cggaatactt tttaatgtcg 2760 atccttctct ggttttctgg atggggtcag gaggcgcatt attgttgctg cttctgttgt 2820 atctggcgcg ccccagtacc agccagacgg caatggttat gaatgcactg ggtgccaatt 2880 cttccctgat ttctaccaga atggtcttca gcctgtttcg tatgcgtcag atgtggatgt 2940 ttgttctata tacgataggt gtggcctgtg tctatgatgt atttgatcag cagtttgcca 3000 tattttttcg ttcatttttt gacactccgc aggcaggaat aaaggcattc ggatttgcca 3060 ccactgcggg ggagatttgt aatgccatta tcatgttctg tacaccatgg ataattaatc 3120 gcattggtgc caaaaatacc ctgcttgttg cggggggaat tatgactatc cgcattaccg 3180 gttctgcttt tgccaccacc atgacagaag tggtgattct gaaaatgctt cacgctcttg 3240 aagttccatt tttgctggtt ggggcattca aatatattac gggcgtgttt gacacccgac 3300 tgtcagcgac tgtttattta attggttttc agttttccaa acaacttgct gcaatacttc 3360 tctctacctt tgccggccac ctgtatgatc gtatgggatt ccagaatacg tattttgtgc 3420 tcgggatgat tgttctgact gttaccgtta tatcagcttt cacgctgagt tcttcccctg 3480 ggatcgtaca cccttctgtt gaaaaagccc ctgtagcaca ttcggagatt aactgatgaa 3540 acagcgtctt tctttggcac agtctgccct tgaaaaactt agcgcacgtc gcggtaatac 3600 ctggtacccg atttttcatc tggctccacc tgccgggtgg atgaatgatc caaatggcct 3660 gatttatttc aatggacgtt accatgcgtt cttccagcat catccagcaa gcgcatatca 3720 ggggccaatg cactgggggc atgccaccag taccgacatg ttgcactggc aacacgagct 3780 tgtcgcgctg gcacccggag ataaatatga tcgtgatggc tgtttttcag ggagtgccgt 3840 ggatgatgat ggcgtgctat cacttattta taccggccat atttgtctcg aagatcgtgg 3900 taatgacagc attatccgtg aagtacagtg tctggctacc agtcatgatg gtattcgctt 3960 tgagaagcag ggctgtgtgc tgacacctcc cgaaggtatt atgcattttc gtgatcccaa 4020 agtctggcac gaagacggct cctggtggat ggtcattggt gcccgggacg cttctgacaa 4080 tgggcaagtt ctgttgtatc gcgggacatc tttgcgggac tggcatctgg agcatgttct 4140 tgctcattcc gcagccggag aaagttatat gtgggaatgc cccgatttct tcaggtgtgg 4200 taattttcac tggctgatgt tctcaccaca ggggatgaac ccttccggtt accggttccg 4260 taaccttttt cagagcggtg ttttggcagg gaactggaag cctggttctg tctttgcgct 4320 gaaaggggta tttgaagagc tggattatgg tcatgacttt tatgccccac agtccatgct 4380 ggctgaggac ggcaggcgta tcattatggc atggatgaat atgtgggatt cacccgtgcc 4440 tacccgtagt gaagcctggg caggatgtct gacgctgccc agagaagttt ttgagcgcga 4500 tggccggctg tgccagcgac ctgtgcgtga agttgaatct ctgcgcagaa aatgccagcc 4560 attatcccct gtaaggttac atggtgtgca attactgacc gaaaatgtac aggccgcaga 4620 attactggtg acgtggcata cggttgacag tcacgcggag cactatggca tccgtctcgg 4680 agaagggctg cggttctatg tggataatca ggccgggcga ctgatactgt ggcgttatta 4740 ccctgaggaa gggctggatg gttaccgcag tgttgaactt cctgatacag aatatctgac 4800 tcttcgtatt ttcctggatc gctcatctgt tgaagtgttt gttaacgatg gtgaggcaac 4860 cttatcaagc cgtatttatc cgcaagcgga ctcgagacaa ttatcgttat atgccgctca 4920 tggcgatgcg atattaactg atggtacttt atggatgctg acctgagaca tatcgatatc 4980 agtatttttt cctgacatta tctgtcacta ctttgtcagt atatagtgtc atacgatgtg 5040 tgaaaataat ggagaatgaa tgcgtaaagt aaaagtatgg actcctgatg atacggttgt 5100 tgatttattg ccggagtcat ctggtaagtt attgccttgt ccggggcggg ggggctggca 5160 gcactggtat ccggggggat gcaagtgaat aaacaggcac tggtagcagc tattgcacag 5220 gttcaatatt gcggcgcatt ggctacaaca gcaaaaggag cgatgacagc actacctcgt 5280 cgac 5284 // |
|