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EBI DbfetchID M24537; SV 1; linear; genomic DNA; STD; PRO; 3360 BP. XX AC M24537; XX DT 06-JUL-1989 (Rel. 20, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 5) XX DE Bacillus subtillis sporulation protein (spoOB), GTP-binding protein (obg), DE phenylalanine biosynthesis associated protein (pheB), and monofunctional DE prephenate dehydratase (pheA) genes, complete cds. XX KW GTP binding protein; obg gene; pheA gene; pheB gene; KW phenylalanine biosynthesis; prephenate dehydratase; spo0B gene; KW sporulation protein; sporulation-associated protein. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3360 RX PUBMED; 2537815. RA Trach K., Hoch J.A.; RT "The Bacillus subtilis spo0B stage 0 sporulation operon encodes an RT essential GTP-binding protein"; RL J. Bacteriol. 171(3):1362-1371(1989). XX FH Key Location/Qualifiers FH FT source 1..3360 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS 58..636 FT /codon_start=1 FT /transl_table=11 FT /gene="spoOB" FT /product="sporulation protein" FT /db_xref="GOA:P06535" FT /db_xref="InterPro:IPR016121" FT /db_xref="PDB:1F51" FT /db_xref="UniProtKB/Swiss-Prot:P06535" FT /protein_id="AAA22504.1" FT /translation="MKDVSKNQEENISDTALTNELIHLLGHSRHDWMNKLQLIKGNLSL FT QKYDRVFEMIEEMVIDAKHESKLSNLKTPHLAFDFLTFNWKTHYMTLEYEVLGEIKDLS FT AYDQKLAKLMRKLFHLFDQAVSRESENHLTVSLQTDHPDRQLILYLDFHGAFADPSAFD FT DIRQNGYEDVDIMRFEITSHECLIEIGLD" FT CDS 670..1956 FT /codon_start=1 FT /transl_table=11 FT /gene="obg" FT /product="GTP-binding protein" FT /note="The GTP-binding site is formed by bases 1162-1182, FT 1302-1314, 1402-1413, 1513-1524." FT /db_xref="GOA:P20964" FT /db_xref="InterPro:IPR005225" FT /db_xref="PDB:1LNZ" FT /db_xref="UniProtKB/Swiss-Prot:P20964" FT /protein_id="AAA22505.1" FT /translation="MFVDQVKVYVKGGDGGNGMVAFRREKYVPKGGPAGGDGGKGGDVV FT FEVDEGLRTLMDFRYKKHFKAIRGEHGMSKNQHGRNADDMVIKVPPGTVVTDDDTKQVI FT ADLTEHGQRAVIARGGRGGRGNSRFATPANPAPQLSENGEPGKERYIVLELKVLADVGL FT VGFPSVGKSTLLSVVSSAKPKIADYHFTTLVPNLGMVETDDGRSFVMADLPGLIEGAHQ FT GVGLGHQFLRHIERTRVIVHVIDMSGLEGRDPYDDYLTINQELSEYNLRLTERPQIIVA FT NKMDMPEAAENLEAFKEKLTDDYPVFPISAVTREGLRELLFEVANQLENTPEFPLYDEE FT ELTQNRVMYTMENEEVPFNITRDPDGVFVLSGDSLERLFKMTDFSRDESVKRFARQMRG FT MGVDEALRERGAKDGDIIRLLEFEFEFID" FT terminator 721..771 FT /gene="spoOB" FT terminator 1970..1999 FT /gene="obg" FT CDS 2017..2460 FT /codon_start=1 FT /transl_table=11 FT /gene="pheB" FT /function="phenylalanine biosynthesis" FT /db_xref="GOA:P21204" FT /db_xref="InterPro:IPR008310" FT /db_xref="UniProtKB/Swiss-Prot:P21204" FT /protein_id="AAA22506.1" FT /translation="MKEETFYLVREDVLPDAMRKTLEVKKLLDRKKADSVADAVQKVDL FT SRSAFYKYRDAVFPFYTMVKEQIITLFFHLEDRSGALSQLLQAVADSGSNVLSIHQTIP FT LQGRANVTLSISTSAMEEDIHTLMNKLRKFDFVEKVEILGSGA" FT CDS 2477..3334 FT /codon_start=1 FT /transl_table=11 FT /gene="pheA" FT /product="prephenate dehydratase" FT /function="phenylalanine biosynthesis" FT /note="monofunctional" FT /db_xref="GOA:P21203" FT /db_xref="InterPro:IPR018528" FT /db_xref="UniProtKB/Swiss-Prot:P21203" FT /protein_id="AAA22507.1" FT /translation="MKVGYLGPAATFTHLAVSSCFQNGAEHVAYRTIPECIDAAVAGEV FT DFAFVPLENALEGSVNLTIDYLIHEQPLPIVGEMTLPIHQHLLVHPSRENAWKELDKIY FT SHSHAIAQCHKFLHRHFPSVPYEYANSTGAAAKFVSDHPELNIGVIANDMAASTYELKI FT VKRDIQDYRDNHTRFVILSPDENISFEVNSKLSSRPKTTLMVMLPQDDQSGALHRVLSA FT FSWRNLNLSKIESRPTKTGLGHYFFIIDIEKAFDDVLIPGAMQELEALGCKVRLLGAYQ FT SYQL" FT terminator 3333..3360 FT /note="For pheB/pheA operon" XX SQ Sequence 3360 BP; 993 A; 651 C; 823 G; 893 T; 0 other; ataatacaca cttatacaga ctcctaaata agaaattaaa tgattgggag tgcgaaaatg 60 aaggatgttt caaaaaatca agaagaaaat ataagcgaca cggcattaac aaacgaactg 120 attcatctgc ttggccattc ccggcatgat tggatgaata agctgcagct gattaaagga 180 aacttaagct tacagaagta tgaccgtgtc tttgaaatga ttgaagaaat ggttatagac 240 gcaaagcacg aatcaaagct ctcaaacctg aaaacaccgc atttggcgtt tgattttctt 300 acgtttaatt ggaaaaccca ttatatgacg cttgaatatg aagttctcgg agaaattaag 360 gatttgtcgg cttatgatca aaagctggcg aaactgatga gaaagctgtt tcatctgttt 420 gatcaagcag tcagcagaga gagtgaaaat catttaacgg tttcgcttca aacggatcat 480 cctgacagac agctgattct gtaccttgat tttcacggcg cctttgccga tccatctgct 540 tttgatgata ttcggcagaa tggatatgag gacgttgata tcatgcgttt tgaaatcacg 600 agtcacgaat gtctgattga aattgggttg gactagcgga gtttttaacg gtttagaacg 660 gaggacatta tgtttgtaga tcaggtcaaa gtatatgtaa aaggcggcga cggcggcaac 720 ggtatggttg cgtttcgccg tgaaaaatat gtgccgaaag gcggccctgc cggcggtgac 780 ggaggaaagg gaggagacgt cgtttttgaa gtagatgaag gtctccgcac cctgatggat 840 tttagataca aaaaacactt taaagcgatt cgcggcgagc atggcatgtc caaaaaccag 900 cacgggcgaa atgctgatga tatggtcatt aaagttccgc cgggcaccgt tgtgacagac 960 gatgatacaa aacaggtcat cgctgattta acagagcacg gacagcgggc tgtcattgca 1020 agaggcggaa gaggcggaag aggaaatagc cgctttgcta caccggctaa tcccgcgcct 1080 cagctttcag aaaacggcga gccgggaaaa gaacgctaca ttgttcttga attaaaagtg 1140 cttgcagatg tcggacttgt cgggttcccg agtgtgggaa aatctacttt gctgtctgtt 1200 gtctcatctg caaaaccgaa aattgcggac tatcacttta caacgcttgt cccgaatctc 1260 ggcatggttg aaacggatga cggacgcagc tttgtcatgg ctgatttgcc agggctgatt 1320 gaaggcgcac accaaggcgt cggactgggc caccagtttt tgcgccatat tgaacggacg 1380 agggttattg ttcatgtcat tgacatgtca ggcttagaag gccgtgatcc atatgatgat 1440 tatcttacga ttaaccagga gctgagcgag tacaatctgc gtctcactga gcgtccgcaa 1500 attatcgttg caaataaaat ggacatgccg gaggctgcgg aaaatctcga agcctttaaa 1560 gaaaagctga cggatgatta tccggtattc ccgatcagtg cggtgacaag agaaggtctg 1620 cgtgagcttc tgtttgaggt tgccaatcag cttgagaaca caccggaatt cccgctgtat 1680 gacgaggaag agcttacaca aaaccgtgtc atgtacacga tggaaaatga agaagtgcca 1740 tttaacatta cacgcgatcc agacggtgta tttgtgcttt caggagacag ccttgagcgg 1800 ttattcaaga tgactgattt ctcacgtgat gaatcggtta aacggtttgc aagacagatg 1860 cgcggaatgg gtgttgatga agcactcaga gaacgcggag ccaaggatgg agatataatc 1920 aggcttctgg aatttgaatt tgaatttatt gattaatcaa tgttggctga aaagagagcc 1980 tggtgccagg ctctctattt taaagggggg atgcaaatga aagaggagac attttatctt 2040 gtccgtgaag atgtattgcc cgatgcaatg agaaaaacat tagaagtcaa aaagctgctt 2100 gatcgaaaaa aagcagattc agtagcagat gccgttcaaa aggtcgattt aagtagaagt 2160 gcgttttata aatacaggga tgctgttttt ccattctaca ccatggtaaa agaacaaatt 2220 atcacacttt tctttcattt ggaggatagg tcaggtgcgt tatctcagct tcttcaggcg 2280 gtagctgatt ctggaagcaa cgttctttcc attcaccaga ccattccgct tcaaggcaga 2340 gcaaatgtga cactgtctat cagtacgtcg gcaatggaag aagacattca tacattaatg 2400 aataagctca ggaagtttga ttttgtagaa aaggttgaaa tattaggttc aggtgcataa 2460 gggagagaaa atcgtcatga aagtcggtta tttaggtcca gcagctacat ttacacatct 2520 agcagtcagt tcttgttttc aaaacggcgc cgaacatgtt gcttaccgca ccattccgga 2580 gtgtatagat gcagccgttg caggcgaagt tgattttgct tttgttcctt tggaaaatgc 2640 tttagaagga tctgttaatc taacaataga ctatttaata catgaacagc ctttgccaat 2700 cgtgggtgaa atgacgttgc cgattcacca gcacttgctc gtccatccct caagagagaa 2760 tgcatggaaa gagctcgaca aaatttactc acattcacac gcgattgcgc aatgccataa 2820 atttcttcat cgacattttc cttccgttcc atatgaatac gccaattcta ccggggcggc 2880 agcaaagttt gtcagtgacc atcccgagct gaatatcggg gtcattgcca atgatatggc 2940 agcttctaca tacgaattaa aaatcgtgaa acgggatata caggattata gggacaatca 3000 tacaagattt gttatcctgt ctcccgatga aaacatatct tttgaagtga attcaaaatt 3060 gagctctagg cccaaaacga ccttaatggt catgctgccg caggatgatc agtccggggc 3120 gctgcataga gtgctgtctg cattttcttg gagaaattta aacctgtcaa aaattgagtc 3180 acgtccgact aaaaccggat taggccatta tttctttatt attgatattg agaaagcgtt 3240 tgatgatgta ttgattccag gggccatgca ggagcttgaa gcactcggct gcaaagtgag 3300 gcttctgggt gcataccagt cttaccaatt ataaaaaaag cccactagag ggcttttttt 3360 // |
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