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EBI DbfetchID M23007; SV 1; linear; genomic DNA; STD; PRO; 6050 BP. XX AC M23007; J05009; J05025; M27145; XX DT 06-JUL-1989 (Rel. 20, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 17) XX DE S.typhimurium NADPH-sulfite reductase flavoprotein component (cysJ), DE NADPH-sulfite reductase hemoprotein component (cysI), and 3' DE phosphoadenosine 5'-phosphosulfate sulfotransferase (cysH) genes, complete DE cds. XX KW 3'-phosphoadenosine-5'-phosphosulfate sulfotransferase; flavoprotein; KW hemoprotein; sulfite reductase. XX OS Salmonella enterica subsp. enterica serovar Typhimurium OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Salmonella. XX RN [1] RP 1-429 RX PUBMED; 2701932. RA Ostrowski J., Kredich N.M.; RT "Molecular characterization of the cysJIH promoters of Salmonella RT typhimurium and Escherichia coli: regulation by cysB protein and RT N-acetyl-L-serine"; RL J. Bacteriol. 171(1):130-140(1989). XX RN [2] RP 2064-6050 RX PUBMED; 2670946. RA Ostrowski J., Wu J.-Y., Rueger D.C., Miller B.E., Siegel L.M., RA Kredich N.M.; RT "Characterization of the cysJIH regions of Salmonella typhimurium and RT Escherichia coli B. DNA sequences of cysI and cysH and a model for the RT siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on RT amino acid homology with spinach nitrite reductase"; RL J. Biol. Chem. 264(26):15726-15737(1989). XX RN [3] RP 370-2194 RX PUBMED; 2550423. RA Ostrowski J., Barber M.J., Rueger D.C., Miller B.E., Siegel L.M., RA Kredich N.M.; RT "Characterization of the flavoprotein moieties of NADPH-sulfite reductase RT from Salmonella typhimurium and Escherichia coli. Physicochemical and RT catalytic properties, amino acid sequence deduced from DNA sequence of RT cysJ, and comparison with NADPH-cytochrome P-450 reductase"; RL J. Biol. Chem. 264(27):15796-15808(1989). XX CC Draft entry computer-readable copy of sequence [2] kindly submitted CC by N.M.Kredich 16-AUG-1989. CC Draft entry and computer-readable sequence for [3] kindly submitted CC by N.M.Kredich, 16-SEP-1989. XX FH Key Location/Qualifiers FH FT source 1..6050 FT /organism="Salmonella enterica subsp. enterica serovar FT Typhimurium" FT /mol_type="genomic DNA" FT /db_xref="taxon:90371" FT mRNA 219..>4717 FT mRNA 227..>4717 FT mRNA 228..>4717 FT CDS 390..2189 FT /codon_start=1 FT /transl_table=11 FT /gene="cysJ" FT /product="NADPH-sulfite reducatase flavoprotein component" FT /EC_number="1.8.1.2" FT /db_xref="GOA:P38039" FT /db_xref="HSSP:1DDG" FT /db_xref="InterPro:IPR001433" FT /db_xref="UniProtKB/Swiss-Prot:P38039" FT /protein_id="AAA27046.1" FT /translation="MTTPAPLTGLLPLNPEQLARLQAATTDLTPEQLAWVSGYFWGVLN FT PRSGAVAVTPVPERKMPRVTLISASQTGNARRVAEALRDDLLAANLNVTLVNAGDYKFK FT QIASEKLLVIVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDTSYEFFCQ FT SGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDVLKSRAPVAAPSQSVATGA FT VNDIHTSPYTKDAPLIATLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWYQ FT NDPALVKELVELLWLKGDEPVTVDGKTLPLAEALEWHFELTVNTANIVENYATLTRSES FT LLPLVGDKAQLQHYAATTPIVDMVRFSPAQLDAQALIDLLRPLTPRLYSIASAQAEVES FT EVHITVGVVRYDIEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPANPETPVIMI FT GPGTGIAPFRSFMQQRAAEGVEGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLSRIDLA FT WSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDARRMAADVEKALLEVIAEFGGM FT DLESADEYLSELRVERRYQRDVY" FT CDS 2189..3907 FT /codon_start=1 FT /transl_table=11 FT /gene="cysI" FT /product="NADPH-sulfite reductase hemoprotein component" FT /EC_number="1.8.1.2" FT /db_xref="GOA:P17845" FT /db_xref="HSSP:7GEP" FT /db_xref="InterPro:IPR005117" FT /db_xref="UniProtKB/Swiss-Prot:P17845" FT /protein_id="AAA27047.1" FT /translation="MSMSEKHPGPLVVEGKLSDAERMKLESNYLRGTIAEDLNDGLTGG FT FKGDNFLLIRFHGMYQQDDRDIRAERAAQKLEPRHAMLLRCRLPGGVITTTQWQAIDKF FT AADNTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSNP FT YESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKTTV FT VIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGYLPL FT EHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGLETFKAEVERRAGIKFEPIRPYE FT FTGRGDRIGWVKGIDDKWHLTLFIENGRILDYPGRPLKTGLLEIAKIHQGEFRITANQN FT LIIASVPESQKAKIETLARDHGLMNAVKPQRENSMACVSFPTCPLAMAEAERFLPSFTD FT KVEAILEKHGIPDEHIVMRVTGCPNGCGRAMLAEIGLVGKAPGRYNLHLGGNRIGSRIP FT RMYKENIAEPDILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPARDFWE" FT repeat_region 3932..3962 FT /note="30-mer repetitive extragenic palindromic sequence" FT CDS 3983..4717 FT /codon_start=1 FT /transl_table=11 FT /gene="cysH" FT /product="3'-phosphoadenosine-5'-phosphosulfate FT sulfotransferase" FT /db_xref="GOA:P17853" FT /db_xref="HSSP:1SUR" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P17853" FT /protein_id="AAA27048.1" FT /translation="MSQLDLNALNELPKVDRVMALAETNAQLEKLSAEERVAWALENLP FT GEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYQFIDELTDKLKLNLKVY FT RAGESPAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELKAQTWFAGLRREQSGS FT RAHLPVLAIQRGVFKVLPIIDWDNRTVYQYLQKHGLKYHPLWDQGYLSVGDTHTTRKWE FT PGMAEEETRFFGLKRECGLHEG" FT terminator 4755..4779 FT CDS complement(4805..5758) FT /codon_start=1 FT /transl_table=11 FT /note="ORF4" FT /db_xref="GOA:P40722" FT /db_xref="UniProtKB/Swiss-Prot:P40722" FT /protein_id="AAA27049.1" FT /translation="MPVTLSFGNHQNYTLNESRLAHLLSADKEKAIHMGGWDKVQDHFR FT AEKKDHALEVLHSIIHGQGRGEPGEMEVNVEDINKIYAFKRLQHLACPAHQDLFTIKMD FT ASQTQFLLMVGDTVISQSNIKDILNISDDAVIESMSREERQLFLQICEVIGSKMTWHPE FT LLQESISTLRKEVTGNAQIKTAVYEMMRPAEAPDHPLVEWQDSLTADEKSVLACINAGN FT FEPTTQFCKIGYQEVQGEVAFSMMHPCISYLLHSYSPFSEFKPTNSGFLKKLNQDYNDY FT HAKKMFIDVILEKLYLTHERSLHIGKDGCSRNILLT" XX SQ Sequence 6050 BP; 1464 A; 1515 C; 1621 G; 1450 T; 0 other; ggtaccggaa agcgcatttt ttatcattaa ctaaaaccat tctccgcctt gtgacgggca 60 attaatttaa gaatttccag aaaatttaat aattatcaat caattataaa atcgattttg 120 gcgttaataa cgataactaa aacaggttag ttcatttggt tatttgttat ttccaaccct 180 tctttaattg ttattcctct caccgttaac cttatcctca gtttgggatt tatcgcttat 240 acatttcatc tttcaagccg catctgtgtt gactgcgttt actcacccca gtcacgtatt 300 tatgtacgct cctggggcgc gatcagctta cggcgaactt gaatgatttt gtgtatagat 360 ccgctttgct tactggaaca taacgacgca tgacgacacc ggctccactg actgggttgc 420 ttccgcttaa tccggagcaa ctggcgcgcc ttcaggccgc gacaaccgac cttactcccg 480 aacagcttgc ctgggtctcc ggctattttt ggggcgtact gaacccgcgc tcaggcgctg 540 ttgccgtcac gcctgttcct gagcggaaga tgccgcgggt tacgcttatc tccgcttcgc 600 aaaccggcaa tgcgcgtcgt gtggcggaag cgctgcgtga tgatctgctg gcggccaacc 660 ttaatgtgac actggtgaac gccggcgact acaaattcaa acagattgcc agtgaaaaac 720 tgttggtgat agtgacgtcc acccagggag aaggcgagcc gccggaagag gccgtcgcgc 780 tgcataaatt cctgttctcc aaaaaagcgc cgaaactgga aaacaccgct tttgccgtgt 840 ttagcctcgg cgacacctct tatgaatttt tctgccagtc cggaaaagat ttcgacagca 900 aactggcgga actgggcggt gaacgtctgc tcgaccgtgt cgatgctgac gttgaatatc 960 aggcagctgc cagcgaatgg cgcgcccgcg tggttgatgt tctgaagtct cgcgcacctg 1020 tcgcggctcc ttcgcaatcc gtcgctactg gcgcggtaaa cgacatccac accagcccct 1080 atactaaaga cgcgccgctg atcgcgaccc tctccgtgaa tcagaaaatc accggtcgca 1140 actcggaaaa agatgtgcgt catattgaga ttgatttagg cgactccggc ctgcgctatc 1200 agccgggcga tgcgctgggc gtctggtatc agaacgatcc ggctctggtg aaggagctgg 1260 ttgagttgct gtggctgaag ggcgatgagc cggtgacggt tgacggtaaa accctgccgc 1320 tcgccgaagc gctggagtgg cacttcgagc tgacggtgaa taccgccaac attgttgaga 1380 actacgcgac cctgacgcgc agtgagtcgc tgttgccatt agtgggcgac aaagcgcagc 1440 tccagcatta cgccgcgacc acgcccattg ttgatatggt gcgtttctct ccggcacagc 1500 tggatgctca ggcgctaatc gacctgctgc gtccgctaac gccgcgtctg tactccatcg 1560 cctccgcaca ggctgaagtg gagagcgaag tgcatatcac cgttggcgtg gttcgttatg 1620 acattgaagg ccgcgcccgg gccggtggcg cttccagctt cctcgccgac cgcgtggaag 1680 aagagggcga agttcgcgtc tttattgagc ataacgataa cttccgcctg ccagctaacc 1740 cggaaacgcc ggtgatcatg attggccccg gtaccggcat cgcgccgttc cgctccttta 1800 tgcaacagcg cgcggcggaa ggggtggaag gtaagaactg gttgttcttc ggcaacccgc 1860 actttaccga agatttcctc tatcaggtgg agtggcagcg ctacgtgaaa gagggggtgt 1920 taagccgtat cgatctcgcc tggtcacgcg atcaaaaaga aaaaatttac gtacaagaca 1980 agctgcgtga acagggcgcc gagctgtggc gctggatcaa tgatggcgcg catatttatg 2040 tctgcggcga cgccaggcgc atggccgcag atgtcgaaaa agcattgctg gaagtgatcg 2100 ctgaattcgg cggtatggac ctcgaatcgg cagatgaata tttaagtgag ctgcgcgtag 2160 agcgccgtta tcagcgagat gtctactaat gagcatgagc gaaaaacatc caggcccact 2220 ggtggtcgaa ggtaaactgt ccgatgccga gcgcatgaag ctggaaagca actatctgcg 2280 cggcaccatt gcggaagatc tgaacgacgg tctcaccggc ggttttaaag gcgataactt 2340 cctgctgata cgctttcatg gcatgtatca gcaggatgac cgcgatattc gcgccgagcg 2400 tgcggcgcag aagctggagc cgcgccacgc gatgctgctg cgctgccgcc tgccgggcgg 2460 ggtgattact acgacgcaat ggcaggcgat cgataaattc gcggcggata acacgattta 2520 cggcagtatc cgtctgacca accgccagac cttccagttc cacggcattc tgaagaagaa 2580 cgttaagccg gtgcaccaga tgctgcactc ggtggggctg gatgcgctgg caaccgccaa 2640 cgacatgaac cgtaacgtgc tgtgtacctc taacccgtac gagtctcagc tgcatgctga 2700 agcctacgaa tgggcgaaga agatctccga acatctgctg ccgcgcaccc gtgcgtatgc 2760 ggagatctgg ctcgatcagg agaaggtcgc gaccaccgat gaagaaccga tcctcgggca 2820 gacctatctg ccgcgtaaat ttaaaaccac ggtggtgatc ccgccgcaaa atgatatcga 2880 tctgcacgcc aacgacatga actttgtggc gattgccgag aacggcaagc tggtcggctt 2940 taacctgctg gtgggcggcg gtctgtctat tgagcatggt aataagaaaa cctacgctcg 3000 tacggccagc gagtttggct atctgccgct ggagcacacc ctggcggtgg ccgaagcggt 3060 agtgacgacg cagcgcgact ggggtaaccg taccgacagg aaaaatgcca aaaccaaata 3120 cacccttgag cgcgtgggac tggagacatt taaagcggaa gtcgagcgtc gcgcggggat 3180 caaatttgag ccgatccgcc cgtatgagtt taccgggcgt ggcgacagga ttggctgggt 3240 gaaaggcatt gatgataaat ggcacctgac gctgtttatt gagaacggcc gtatcctgga 3300 ttatccgggg cgtccgctga aaaccggcct gctggagatc gcgaagattc atcagggcga 3360 gttccgtatc accgctaacc agaacctgat tattgccagc gtgccggaaa gccagaaagc 3420 gaagatcgag acgctggcgc gcgaccacgg gttgatgaat gcggtgaaac cgcagcgtga 3480 aaactcaatg gcctgcgtgt cgttcccaac ctgcccgctg gcaatggcgg aagccgagcg 3540 tttcctgccg tcgttcacgg acaaagtgga agcgattctg gaaaaacacg gcattcctga 3600 tgagcatatc gtgatgcgcg tgaccggctg cccgaacggc tgtggccgcg cgatgctggc 3660 ggaaattggc ttagtaggga aagcgcctgg tcgttataac ctgcatcttg gtggcaaccg 3720 catcgggagt cgtatcccac ggatgtataa agaaaatatc gccgagccgg atattctggc 3780 ctcgctggat gaactgattg ggcgctgggc gaaagagcgt gaagcgggtg aaggcttcgg 3840 cgactttacg gtgcgtgcgg gcatcattcg cccggtgctc gatcccgcgc gtgatttctg 3900 ggaataacct gaattgccgg atggcgtctg tgtaggcctg ataagcacag cgccatcagg 3960 caaggcaaac agtgaggaac tcatgtccca actcgatctc aacgcattga atgaattgcc 4020 aaaagtcgat cgcgtaatgg ctctggcgga aaccaacgcc caactggaaa aactgagcgc 4080 cgaagaacgc gtggcctggg cgctggaaaa tctgcccggc gaatacgtgc tctcctcaag 4140 ctttggcatc caggcggcgg tgagcctgca cctggtgaac cagattcgcc cggatatccc 4200 ggtgatcctc accgataccg gctatttgtt cccggaaacc taccagttta ttgatgagct 4260 aacggacaaa ctcaaactca acctgaaagt ttaccgcgcg ggcgaaagtc cggcctggca 4320 ggaagcgcgc tacggcaagc tgtgggagca gggcgttgag ggcattgaga aatacaatga 4380 gatcaacaaa gtcgagccga tgaaccgggc gctaaaagag ctgaaagcgc agacgtggtt 4440 tgcgggtctg cgtcgcgagc agtccggcag ccgcgcgcat ttacccgttc tcgctatcca 4500 gcgcggcgtg tttaaagtgc taccgattat cgactgggat aatcgcaccg tttaccagta 4560 cctgcaaaag catggcctga aataccatcc gctgtgggat cagggctatc tgtcggtggg 4620 cgatacgcac accacccgca aatgggagcc gggtatggcg gaagaagaga cacgcttctt 4680 cgggctgaag cgcgagtgcg ggctgcatga agggtaattg tccttacgcg gtatgattat 4740 acttgctgct atgaaaaatc cggcaggcag ccggatttta aattggttat attactgact 4800 atctttatgt cagtaatata ttacgactgc acccatcttt accaatgtgc aaagaacgtt 4860 catgggttag atatagtttt tcaagaatta catcaataaa catttttttt gcatgatagt 4920 cgttataatc ctgattaagt ttttttaaaa aaccagaatt tgttggttta aactctgaaa 4980 acggtgaata gctatgtagt aaataggaga tgcacggatg catcatgcta aaagcgactt 5040 caccttgtac ctcctgataa cctattttgc aaaactgagt cgtaggctca aagttaccgg 5100 cattaataca ggccagcact gatttttcat ctgcagtaag tgagtcctgc cattcgacaa 5160 gcgggtggtc tggagcctct gcgggacgca tcatctcata aaccgccgtt ttgatttgtg 5220 catttcccgt cacttctttt cgtagagttg aaatcgactc ctgaagtaat tccgggtgcc 5280 aggtcatctt cgaaccaatc acctcacata tttgtaaaaa taattgtctc tcctctctac 5340 tcatagattc tatcactgca tcatctgata tattaaggat gtccttaata ttgctttggc 5400 tgatcaccgt gtcgccgacc ataagtaaaa attgtgtctg agaagcgtcc atcttgatag 5460 taaacagatc ttgatgagcc gggcaggcca gatgttgtag ccgtttaaag gcgtaaattt 5520 tatttatatc ctctacgtta acttccatct ctccaggttc accacggccc tgcccgtgaa 5580 tgatggagtg taacacttcc agcgcatggt cctttttttc cgctctaaaa tgatcctgga 5640 ctttatccca accccccata tggattgctt tttctttatc tgcgcttaac agatgagcaa 5700 gccgactttc attaagcgta taattttgat gattaccgaa gcttaaagtg actggcataa 5760 tattttcctt caaatttata ttattcaata tccggagtaa aatatatggt tcaattctct 5820 cttttttatt aaacagataa agtttactac aggagagaat ttgaacgaca ttattaatac 5880 agcacattta tggttggtaa tcctttgtgg tgactataat tcagacaaaa ggtagatgat 5940 tcgccgcaaa tggtcgtttt ttttaaattt tggtttatgc agaaggcatt ggaggagcct 6000 ctttgagcgc ttcttcctgt tgaaaaggcg gaaaatttat aataattatt 6050 // |
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