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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M22852; SV 1; linear; genomic DNA; STD; INV; 2396 BP.
XX
AC   M22852; J03973;
XX
DT   23-NOV-1989 (Rel. 21, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   C.fasciculata dihydrofolate reductase-thymidylate synthetase (DHFR-TS)
DE   gene, complete cds.
XX
KW   dihydrofolate reductase-thymidylate synthetase.
XX
OS   Crithidia fasciculata
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Crithidia.
XX
RN   [1]
RP   1-2396
RX   DOI; 10.1016/0166-6851(89)90007-8
RX   PUBMED; 2540436.
RA   Hughes D.E., Shonekan O.A., Simpson L.;
RT   "Structure, genomic organization and transcription of the bifunctional
RT   dihydrofolate reductase-thymidylate synthase gene from Crithidia
RT   fasciculata";
RL   Mol. Biochem. Parasitol. 34(2):155-166(1989).
XX
CC   Draft entry and computer-readable sequence for [1] kindly submitted
CC   by L.Simpson, 06-MAR-1989.
CC   This is not the primary transcript as it has a trans-spliced 5' 39
CC   nucleotide miniexon sequence.  The sequence downstream of the
CC   putative DHRF promoter consensus sequence which is complementary to
CC   the 3' end of the C.fasciculata miniexon-derived RNA suggests a
CC   means by which the two separately transcribed RNAs may be
CC   juxtaposed for trans-splicing.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2396
FT                   /organism="Crithidia fasciculata"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:5656"
FT   misc_feature    378
FT                   /note="miniexon splice site"
FT   CDS             498..2045
FT                   /codon_start=1
FT                   /note="dihydrofolate reductase-thymidylate synthetase"
FT                   /db_xref="GOA:Q23695"
FT                   /db_xref="InterPro:IPR000398"
FT                   /db_xref="InterPro:IPR001796"
FT                   /db_xref="InterPro:IPR012262"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q23695"
FT                   /protein_id="AAA30318.1"
FT                   /translation="MSRAAAKFKIPMPVTKADFAFPSLRAFSIVVAADQQHGIGDGETI
FT                   PWTVPETLAFFKDQTTLLRNKKPPTEKKRNAVVMGRKTWEVPLKFRPLKGRLNVVLSCR
FT                   RPVDDLLAQLPEEKRAAAAADVVINGGLKEALHLLARPPYCSSIETAYCIGGARVYTEA
FT                   MQSPCVEKLKEVYLTRVHTAPTCNRFYEFVRRRRARAAVDLESTSGVKVSDAAAHLSYE
FT                   IMKYVPHNAEERQYLELIDRIMKTGLVKEDRTGVGTISLFGAQMFSLRDNQLPLLTTKR
FT                   VFWRGVCEELIWFLRGETNAHVLADKDIHIWDGNGSREFLDSRGLTENKEMDLGPVYGF
FT                   QWRHFGADYKGFDANYDEGVDQIKTIVETLKTNDRRLLVTAWNPCALHKMAVRPCHLLG
FT                   QFYVNTQTKELSCMLYQRCCDMGLGVPFNIASYALLTILIAKATGLRPGELVHTLGTAH
FT                   VYSNHVEALKEQLQRVPVAFPVLVFKKEREFLEDYESTDMEVVDYVPYPPIKMEMAV"
XX
SQ   Sequence 2396 BP; 535 A; 689 C; 684 G; 488 T; 0 other;
     tagcttgtag agagaacggc aaaaatctta tttcttctct cttcccgcgt tgacttcctt        60
     ttctagcgtg ccttttttta ttattggagg cagtcaacta aatagaagta gtggacgaag       120
     aagcccttac ggcgcgggtg gcaagcgttc ttcctcgctc ttgccatccg ccgcacgccg       180
     cttgcgacct ccactgcacg ccccacaact ccactacccc acttccctct ctctgtctct       240
     acgtgttaag acttcctctc gaccagccat catccaagca gcttcacgca caaacaccac       300
     aagaaaaaaa gaaggcaaaa aaaaaagagt gtagcaccag taagacacac cacaaaaaaa       360
     aaaaaaaaaa gaatatagag taagctgtca gttgagccat acattacttc agaaattcaa       420
     caatcctcat cacacaactc cccatcaaca gcaacaataa agaacagaaa ccccataacg       480
     ccaactcgtt ctcaaagatg tcacgcgcag ctgccaagtt caagatcccg atgccggtca       540
     ccaaggccga cttcgccttc ccctccctcc gcgccttcag catcgtcgtc gcggccgatc       600
     agcagcacgg tattggcgac ggcgagacga ttccgtggac ggtgccggag acactggcct       660
     tcttcaagga ccagacgacg ctgctgcgca acaagaagcc cccgacggag aagaagcgca       720
     acgctgtggt gatgggccgc aagacgtggg aagtgccgct caagttccgt cctctcaagg       780
     gtcgcctgaa cgtggtgctc tcctgcaggc gaccggtgga cgaccttctg gcgcagctgc       840
     cagaggagaa gcgcgctgcc gccgctgccg atgtcgtgat caacggtggg ctgaaggagg       900
     ccctgcacct gctggctcgt ccgccgtact gcagttcgat tgagacggcg tactgcatcg       960
     gcggcgcgcg ggtctacaca gaggcgatgc agtccccgtg tgtggagaag ttgaaggagg      1020
     tgtacctgac ccgcgtgcac acggcgccga cgtgcaaccg cttctacgag ttcgtacggc      1080
     ggagaagagc gcgcgcagca gtggacctgg agtccacctc tggcgtgaag gtgagcgacg      1140
     cagcggcaca cctgtcgtat gagattatga agtacgtgcc acacaacgct gaggagcggc      1200
     agtacctgga gctcatcgac cgcatcatga agaccgggct ggtgaaggag gaccgcaccg      1260
     gcgtcggcac catcagcctc ttcggcgcgc agatgttctc gctgcgggac aaccagctgc      1320
     cgctgctgac gacgaagcgc gtcttctggc gtggcgtctg cgaagagctc atctggttcc      1380
     tccgtggcga gacaaacgcg cacgtgctgg cagacaagga cattcacatc tgggacggca      1440
     acggctcccg cgagttcctc gacagccgtg ggctaacgga gaacaaagag atggaccttg      1500
     ggccggtgta cggctttcag tggcgacact tcggggcaga ctacaaaggc ttcgacgcga      1560
     attacgacga gggcgtggat cagatcaaga cgattgttga gaccctcaag acgaacgacc      1620
     gccgtctgct cgttacggcg tggaacccgt gcgcgctgca caagatggcc gtgcggccct      1680
     gccacctcct cggccagttc tatgtgaaca cgcagacgaa ggagctgtcc tgcatgctgt      1740
     accagcgctg ctgcgacatg ggtctcggcg tgcccttcaa catcgcgtcg tacgccttgc      1800
     tgacgattct gattgccaag gcgaccgggc tgcgtcctgg agagctcgtg cacaccctcg      1860
     gtaccgcgca tgtgtacagc aaccacgtgg aggcgctgaa ggagcagctg cagcgggtgc      1920
     ccgtggcctt ccccgtgctc gtcttcaaga aggagcgcga gtttttggag gactacgagt      1980
     cgacggacat ggaggtggtc gactacgtgc cctacccgcc catcaagatg gagatggctg      2040
     tctaagtcag tacttcttgt ttgtgtgtgt gtgtgtgtgt gtgtgtgtgt ggacatccca      2100
     acatagattc tttagccgtc cgcacagtgc gaaggtgccg aacacccgaa cgcgtccttt      2160
     ccgactcaat ttttttctct cgagtcttga gttgcgttgg gctgatagaa gaggggacac      2220
     agaccaagga aaacctgccg cgttggcgct gcacggcttc tttctgtaaa ggacgctcgt      2280
     aggtgggttt gattgtgcca agcggtcttt cttttacttt gttgttgaag aggcggtaga      2340
     agacgcagag agcggttacg cagatctgca agttttctat gctcttcgtc aagctt          2396
//
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