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EBI DbfetchID M22759; SV 1; linear; genomic DNA; STD; PRO; 3471 BP. XX AC M22759; XX DT 06-JUL-1989 (Rel. 20, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Clostridium thermocellum celX gene, 3' end and endo-beta-1, 4-glucanase DE (celE) gene, complete cds. XX KW endo-beta-1,4-glucanase; endoglucanase; xylan-hydrolysing activity. XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3471 RX DOI; 10.1016/0378-1119(88)90375-7 RX PUBMED; 3066698. RA Hall J., Hazlewood G.P., Barker P.J., Gilbert H.J.; RT "Conserved reiterated domains in Clostridium thermocellum endoglucanases RT are not essential for catalytic activity"; RL Gene 69(1):29-38(1988). XX FH Key Location/Qualifiers FH FT source 1..3471 FT /organism="Clostridium thermocellum" FT /mol_type="genomic DNA" FT /db_xref="taxon:1515" FT gene 1..676 FT /gene="celX" FT CDS <1..676 FT /codon_start=2 FT /transl_table=11 FT /gene="celX" FT /note="product homologous to other C.thermocellum FT endoglucanases; putative" FT /db_xref="GOA:P15329" FT /db_xref="InterPro:IPR001087" FT /db_xref="PDB:2VPT" FT /db_xref="UniProtKB/Swiss-Prot:P15329" FT /protein_id="AAA23223.1" FT /translation="IMKVTQDGSIPQIASNINNWLNTHNPDVVFLWIGGNDLLLSGNVN FT ATGLSNLIDQIFTVKPNVTLFVADYYPWPEAVKQYNAVIPGIVQQKANAGKKVYFVKLS FT EIQFDRNTDISWDGLHLSEIGYTKIANIWYKYTIDILKALAGQTQPTPSPSPTPTDSPL FT VKKGDVNLDGQVNSTDFSLLKRYILKVVDINSINVTNADMNNDGNINSTDISILKRILL FT RN" FT -10_signal 897..902 FT /gene="celE" FT gene 897..3471 FT /gene="celE" FT CDS 1027..3471 FT /codon_start=1 FT /transl_table=11 FT /gene="celE" FT /product="endo-beta-1,4-glucanase" FT /db_xref="GOA:P10477" FT /db_xref="InterPro:IPR001087" FT /db_xref="PDB:2WAB" FT /db_xref="UniProtKB/Swiss-Prot:P10477" FT /protein_id="AAA23224.1" FT /translation="MKKIVSLVCVLVMLVSILGSFSVVAASPVKGFQVSGTKLLDASGN FT ELVMRGMRDISAIDLVKEIKIGWNLGNTLDAPTETAWGNPRTTKAMIEKVREMGFNAVR FT VPVTWDTHIGPAPDYKIDEAWLNRVEEVVNYVLDCGMYAIINLHHDNTWIIPTYANEQR FT SKEKLVKVWEQIATRFKDYDDHLLFETMNEPREVGSPMEWMGGTYENRDVINRFNLAVV FT NTIRASGGNNDKRFILVPTNAATGLDVALNDLVIPNNDSRVIVSIHAYSPYFFAMDVNG FT TSYWGSDYDKASLTSELDAIYNRFVKNGRAVIIGEFGTIDKNNLSSRVAHAEHYAREAV FT SRGIAVFWWDNGYYNPGDAETYALLNRKTLSWYYPEIVQALMRGAGVEPLVSPTPTPTL FT MPTPSPTVTANILYGDVNGDGKINSTDCTMLKRYILRGIEEFPSPSGIIAADVNADLKI FT NSTDLVLMKKYLLRSIDKFPAEDSQTPDEDNPGILYNGRFDFSDPNGPKCAWSGSNVEL FT NFYGTEASVTIKSGGENWFQAIVDGNPLPPFSVNATTSTVKLVSGLAEGAHHLVLWKRT FT EASLGEVQFLGFDFGSGKLLAAPKPLERKIEFIGDSITCAYGNEGTSKEQSFTPKNENS FT YMSYAAITARNLNASANMIAWSGIGLTMNYGGAPGPLIMDRYPYTLPYSGVRWDFSKYV FT PQVVVINLGTNDFSTSFADKTKFVTAYKNLISEVRRNYPDAHIFCCVGPMLWGTGLDLC FT RSYVTEVVNDCNRSGDLKVYFVEFPQQDGSTGYGEDWHPSIATHQLMAERLTAEIKNKL FT GW" FT sig_peptide 1027..1128 FT /gene="celE" FT mat_peptide 1129..3468 FT /gene="celE" FT /product="endo-beta-1,4-glucanase" XX SQ Sequence 3471 BP; 1074 A; 632 C; 811 G; 954 T; 0 other; gatcatgaag gtcactcagg atggatcgat tccccagata gcaagcaata tcaacaactg 60 gcttaatacc cataacccgg atgtggtatt tctgtggatt ggaggaaatg acctgctttt 120 gagcggaaac gtgaatgcaa caggccttag taatcttata gaccagattt tcacagtgaa 180 acccaatgta acactgtttg tggccgatta ttatccgtgg cctgaagcgg tcaagcaata 240 caatgcggtg attccgggaa tagttcaaca gaaggccaat gccggcaaga aagtttattt 300 tgtaaagctt agtgagattc agtttgacag gaacaccgat atttcatggg atggtttgca 360 cttgagcgaa ataggataca caaagattgc aaatatttgg tacaagtata cgattgacat 420 actaaaagct ttggcaggac aaacgcagcc aactccaagt ccgtctccga ctcccacaga 480 ttctcctctg gttaaaaaag gtgatgttaa tttggacggt caggtcaatt cgacagattt 540 cagccttttg aaaagatata tactgaaagt tgtggatata aattcaataa atgtgacaaa 600 tgctgatatg aacaatgatg gcaatatcaa ctctacagac atttcaatac taaagagaat 660 acttcttaga aattagaaaa tatattgtgg atttttaaag tttttaataa acggatatat 720 aaaagaagct ccaaattctt tggagcttct tttatatatc ccgggtttcc aacaaagtgc 780 aacctttttt cagcagaggt tactatgttt ttaattattt ttttgctttg cgctgtccaa 840 aagaaaatga tttttcgaat taatataata aacagtaaca ttaccgttta gtttgttata 900 atgttttatt tcaagccaaa taggaagggg gagctgtaag aataaagttg attgatgcaa 960 gaaaagagga tttaaagtaa aagattattg ttttttgtaa aggatttttg taaaggagag 1020 ggtaatgtga aaaaaattgt ttctttggtt tgtgtgcttg tgatgctggt aagcatctta 1080 ggctcgtttt cagtcgtagc ggcatcaccg gtaaaaggct ttcaggtatc gggaacaaag 1140 cttttggatg caagcggaaa cgagcttgta atgaggggca tgcgtgatat ttcagcaata 1200 gatttggtta aagaaataaa aatcggatgg aatttgggaa atactttgga tgctcctaca 1260 gagactgcct ggggaaatcc aaggacaacc aaggcaatga tagaaaaggt aagggaaatg 1320 ggctttaatg ccgtcagagt gcctgttacc tgggatacgc acatcggacc tgctccggac 1380 tataaaattg acgaagcatg gctgaacaga gttgaggaag tggtaaacta tgttcttgac 1440 tgcggtatgt acgcgatcat aaatcttcac catgacaata catggattat acctacatat 1500 gccaatgagc aaaggagtaa agaaaaactt gtaaaagttt gggaacaaat agcaacccgt 1560 tttaaagatt atgacgacca tttgttgttt gagacaatga acgaaccgag agaagtaggt 1620 tcacctatgg aatggatggg cggaacgtat gaaaaccgag atgtgataaa cagatttaat 1680 ttggcggttg ttaataccat cagagcaagc ggcggaaata acgataaaag attcatactg 1740 gttccgacca atgcggcaac cggcctggat gttgcattaa acgaccttgt cattccgaac 1800 aatgacagca gagtcatagt atccatacat gcttattcac cgtatttctt tgctatggat 1860 gtcaacggaa cttcatattg gggaagtgac tatgacaagg cttctcttac aagtgaactt 1920 gatgctattt acaacagatt tgtgaaaaac ggaagggctg taattatcgg agaattcgga 1980 accattgaca agaacaacct gtcttcaagg gtggctcatg ccgagcacta tgcaagagaa 2040 gcagtttcaa gaggaattgc tgttttctgg tgggataacg gctattacaa tccgggtgat 2100 gcagagactt atgcattgct gaacagaaaa actctctcat ggtattatcc tgaaattgtc 2160 caggctctta tgagaggtgc cggcgttgaa cctttagttt caccgactcc tacacctaca 2220 ttaatgccga ccccctcgcc cacggtgaca gcaaatattt tgtacggtga cgtaaacggg 2280 gacggaaaaa taaattctac agactgtaca atgctaaaga gatatatttt gcgtggcata 2340 gaagaattcc caagtcctag cggaattata gccgctgacg taaatgcgga tctgaaaatc 2400 aattccaccg acttggtatt gatgaaaaaa tatctactgc gctcaataga caaatttcct 2460 gcggaggatt ctcaaacacc tgatgaagac aatccgggca ttttgtataa cggaagattc 2520 gatttttcag atccgaacgg tccgaaatgc gcctggtccg gcagcaatgt tgagctgaat 2580 ttttacggca cggaagcaag tgtgactatc aaatccggcg gtgagaactg gttccaggct 2640 attgtagacg gcaatcctct tcctcctttt tcggttaacg ctactacctc taccgtaaag 2700 cttgtaagcg gtcttgcaga aggagctcat catcttgtat tgtggaagag gacagaggca 2760 tccttgggag aagttcagtt ccttgggttt gattttggtt caggaaagct tcttgccgca 2820 ccgaagcctt tggaaagaaa gattgagttt atcggagact ccatcacatg tgcatacgga 2880 aatgaaggaa caagcaagga gcagtctttt acaccgaaaa atgaaaacag ctatatgtct 2940 tatgcggcaa ttacagcccg taatttgaat gcaagtgcaa atatgattgc gtggtccgga 3000 atcggactta ccatgaacta cggcggagcc cccggacctc ttataatgga ccgttatcct 3060 tatacccttc cttacagcgg agtcagatgg gattttagca aatatgtgcc tcaggttgtt 3120 gtaatcaatc ttggtaccaa tgatttttct acatcatttg cagataaaac aaagtttgta 3180 acggcatata aaaaccttat aagtgaagtt cgcaggaact atccggatgc ccatatattc 3240 tgctgtgtcg gtccgatgct ttggggaacg ggcctggatt tgtgccgcag ttatgttacg 3300 gaagttgtaa atgattgtaa cagaagcggg gatttaaagg tgtattttgt tgagtttccg 3360 cagcaggacg gaagcaccgg atacggagaa gactggcatc caagtattgc cacccaccag 3420 ctgatggctg agcggcttac tgcggaaata aaaaacaagc ttggatggta a 3471 // |
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