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EBI DbfetchID M20659; SV 1; linear; genomic DNA; STD; PRO; 3932 BP. XX AC M20659; XX DT 20-FEB-1989 (Rel. 18, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE B.subtilis hutH and hutP genes, encoding histidase and a positive DE regulatory protein, complete cds. XX KW histidase; positive regulatory protein. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3932 RX PUBMED; 2454913. RA Oda M., Sugishita A., Furukawa K.; RT "Cloning and nucleotide sequences of histidase and regulatory genes in the RT Bacillus subtilis hut operon and positive regulation of the operon"; RL J. Bacteriol. 170(7):3199-3205(1988). XX DR GOA; P42293. DR InterPro; IPR000193; Urocanase. DR PDB; 2FKN; X-ray. DR UniProtKB/Swiss-Prot; P42293; YXIA_BACSU. XX CC Draft entry and computer-readable sequence [1] kindly submitted by CC M.Oda, 14-SEP-1988. CC The hutP1 mutation causes a pleiotropic loss of all four hut CC enzymes but does not affect the histidine transport system. XX FH Key Location/Qualifiers FH FT source 1..3932 FT /organism="Bacillus subtilis" FT /strain="W168" FT /sub_strain="PY79" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT mRNA 1089..>3932 FT /note="hut mRNA" FT CDS 1121..1576 FT /codon_start=1 FT /transl_table=11 FT /note="hut operon positive regulatory protein" FT /db_xref="GOA:P10943" FT /db_xref="InterPro:IPR015111" FT /db_xref="PDB:1VEA" FT /db_xref="UniProtKB/Swiss-Prot:P10943" FT /protein_id="AAA22537.1" FT /translation="MIHMTLHKERRIGRLSVLLLLNEAEESTQVEELERDGWKVCLGKV FT GSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVG FT LRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI" FT variation 1302 FT /note="c in wild type; t in hutP1 mutant" FT CDS 1689..3215 FT /codon_start=1 FT /transl_table=11 FT /note="histidase" FT /db_xref="GOA:P10944" FT /db_xref="HSSP:1GKM" FT /db_xref="InterPro:IPR001106" FT /db_xref="UniProtKB/Swiss-Prot:P10944" FT /protein_id="AAA22538.1" FT /translation="MVTLDGSSLTTADVARVLFDFEEAAASEESMERVKKSRAAVERIV FT RDEKTIYGINTGFGKFSDVLIQKEDSAALQLNLILSHACGVGDPFPECVSRAMLLLRAN FT ALLKGFSGVRAELIEQLLAFLNKRVHPVIPQQGSLGASGDLAPLSHLALALIGQGEVFF FT EGERMPAMTGLKKAGIQPVTLTSKEGLALINGTQAMTAMGVVAYIEAEKLAYQTERIAS FT LTIEGLQGIIDAFDEDIHLARGYQEQIDVAERIRFYLSDSGLTTSQGELRVQDAYSLRC FT IPQVHGATWQTLGYVKEKLEIEMNAATDNPLIFNDGDKVISGGNFHGQPIAFAMDFLKI FT AISELANIAERRIERLVNPQLNDLPPFLSPHPGLQSGAMIMQYAAASLVSENKTLAHPA FT SVDSIPSSANQEDHVSMGTIAARHAYQVIANTRRVIAIEAICALQAVEYRGIEHAASYT FT KQLFQEMRKVVPSIQQDRVFSYDIERLTDWLKKESLIPDHQNKELRGMNI" XX SQ Sequence 3932 BP; 1042 A; 870 C; 1015 G; 1005 T; 0 other; gtcgacgaca ttcggatgtt tggttgcatc atacggtgtt ccatcgctgc ttgtgccttc 60 cattcctgat ttcaaaaaga tgcctttgtt tttgtatggg ccttcgatgt tgtcagcaac 120 ggcaacaccc atggcggatc ttggcgaatc gccgcggcag gcgttgtagt acatgtagta 180 tttgccgtcc gccagctgcg tgacgtcagc cgcccaaaga gtatcggatt gcgcccattc 240 gaaggtttct tttaattctt catagacatt cgggataagt gggttgtcat tgcttacgct 300 ggtggtcagc tgctgccatt gcatgaggtc attcgattta gctgaagcga gatgagatcc 360 aaaaacataa aaagtgccgt ttgtttcaat gatggaaggg tcatggacgc tgacttcttt 420 aaatataggt ttttgtgcat agacggagtt tgggagggtg aaggccatga taagagctgc 480 aagaaaacat acacggaaca atcggttgaa catctaaaca attcgcctct cttttttatt 540 aaattttaaa cgcttacatt tctctatgaa gataccccgg cctattaaaa cagccgaatg 600 ttttcatatt catccagata ggtgatttct cacttcactt acatagtcag actgatgtct 660 gtctgttttg cgcagaactt gttgcttgca tatcctctcc ttcatagttc aactcttttg 720 ctgtttctcc ttcttgcatc acgtctgttt ttctttcgcg tacatgtaat aactgctttg 780 tcaggttttg catcaactgt gatacagcca tttcgttgtc ggcgtttttt aagatgatgc 840 ctgattgccc gtcaacacca gagtcattcg gctggctctt tataaaacgc cttgcgaaaa 900 atacgactta gtaaacgcat attgagagtt agacagcggc gcttgcctcc ttgtctggat 960 atcgtgtgca atcgctgaaa aaacctgttc tgctttctgc gtcacgctat tacaatagca 1020 atctaacttg ttaaagacta taaaaaaacc ttttgacttc tgctgctgaa ccaattaata 1080 taatactcag ttaatagtta tcagaatttt taggaattga gtgattcata tgacactgca 1140 taaagagcgt cggatcggcc ggctgtctgt tctcctgctg ctgaatgagg cggaagaaag 1200 tacgcaggtt gaggagctgg agcgagacgg atggaaggtc tgtcttggca aggtaggatc 1260 aatggacgca cataaagtag tagccgcaat tgaaaccgct tccaaaaaga gcggtgtcat 1320 tcaatctgag ggttatcggg agtcacatgc gctttatcat gcgacgatgg aggctttgca 1380 tggcgtgacc agaggtgaaa tgctgctggg atcgctgctt cggacggtgg gattgaggtt 1440 tgccgttttg aggggaaatc cttatgaaag tgaagcggaa ggcgattgga tcgctgtctc 1500 gctttacgga acaatcgggg cgccgattaa aggtcttgag catgaaacat tcggcgttgg 1560 aattaatcac atatgaaaca gcccatagat cttagacgat agggggctat gcgtgaaaac 1620 agaagttcac agcatagctc ctttttgtat gggcgcttta ccaaagtaaa gaaaaaggag 1680 ttgggcttat ggtgacttta gacggttctt cattgacaac agccgacgtc gctcgtgtgc 1740 tgtttgattt tgaggaggca gcggcttctg aggaaagtat ggagcgcgtg aaaaaaagca 1800 gagcagcagt ggaacggatt gtacgcgacg aaaagacaat ctacggcatc aacaccggat 1860 ttggcaaatt cagcgatgtc ctgattcaga aagaagactc ggcggcgctg cagctgaatt 1920 taatcctttc tcatgcctgc ggagtcggcg acccttttcc tgagtgtgtt tccagggcaa 1980 tgctgctttt gcgcgccaat gcgctgttaa aaggcttttc cggtgtacgc gctgagctga 2040 tcgagcagtt gcttgctttt ttaaataaac gcgtccatcc tgtcatccct cagcaaggat 2100 cacttggcgc aagcggagat ctggcgccgc tttcccactt ggcgcttgcg ctgatcggac 2160 aaggagaagt cttttttgag ggggaaagaa tgccggcaat gacaggcttg aaaaaagcag 2220 gtattcaacc ggtaacgctg acatccaagg aagggcttgc cttgattaac gggactcagg 2280 ccatgactgc aatgggggtg gtcgcctaca tcgaagctga gaaactagcc tatcaaacag 2340 aacgaattgc cagcttaacg attgaggggt tgcagggaat tatcgatgca tttgacgagg 2400 atattcactt agcccgcggc tatcaggagc agatcgatgt cgcagaacga attcgctttt 2460 atctatctga cagcggcctg acaacctccc agggagaact gcgggtacag gatgcctatt 2520 cattgcgctg tattccgcaa gtacacggtg cgacttggca gacactagga tatgtcaaag 2580 aaaaattaga gattgaaatg aacgcagcta cagacaatcc actcattttc aatgacggtg 2640 acaaagtgat ttccggcgga aactttcacg gacagccaat cgcctttgcc atggattttc 2700 tgaaaatcgc aatatcagag cttgcgaata tcgcagagcg taggatcgaa cgacttgtga 2760 atccgcagct gaatgacctg ccgccgttcc tgagccctca tcccggtctg caatccggtg 2820 caatgatcat gcagtacgcc gcggcctcac tcgtttctga aaataaaaca ctggcccatc 2880 ctgcaagtgt ggattccatt ccgtcatccg ccaaccaaga ggaccatgtc agcatgggaa 2940 ccatcgccgc tcggcatgct tatcaggtga tcgcaaatac aaggagagtc attgcgatcg 3000 aagccatttg cgcattgcag gctgttgaat acagaggcat agaacatgca gcgtcctata 3060 cgaaacagct ttttcaagaa atgagaaaag tcgtgccgtc cattcagcag gaccgtgttt 3120 tttcctatga cattgaacgc ttgactgact ggctgaagaa agaatcttta atacctgatc 3180 atcagaataa agaactaagg gggatgaaca tatgactgat gtaaaaaaat caatccgtgc 3240 caacagagga acagagcttg aatgcttagg atgggagcaa gaggcagtgc ttcggatgct 3300 tcgaaacaac cttgatccag aagtcgccga gaagccggag gacctcatcg tatatggcgg 3360 gattggcaaa gccgcacggg attgggacgc ttttcacgcc attgagcatt ccttgaaaac 3420 attaaaaaat gatgaaacat tgctcgtgca atccggaaag cccgttggca tgttccgtac 3480 acacccgcag gcccctcgcg tgctcctagc aaattctgtt cttgttccga agtgggcaga 3540 ctgggagcat ttccacgaac ttgagaaaaa agggttaatg atgtacggac agatgacggc 3600 tggcagctgg atttacattg gctcacaagg catattgcaa ggaacctatg aaaccttcgc 3660 tgagctggcg cgacagcact tcgggggcag cttaaaagga acgttgacgc tgacagcggg 3720 tctcggcgga atgggaggcg cccagccgct gtcagtcacg atgaatgaag gcgtcgtgat 3780 tgccgtcgag gttgacgaga aacgcatcga caagcggatc gaaacaaaat actgcgatcg 3840 aaaaacagca tcgattgagg aagccttagc ttgggctgaa gaggcaaagc tggcaggaaa 3900 acctttgtcc attgcacttc tcggaaacgc ag 3932 // |
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