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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M20012; SV 1; linear; genomic DNA; STD; PRO; 2095 BP.
XX
AC   M20012;
XX
DT   20-FEB-1989 (Rel. 18, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   B.subtilis thymidylate synthase B and dihydrofolate reductase genes.
XX
KW   dfrA gene; dihydrofolate reductase; thyB gene; thymidylate synthase B.
XX
OS   Bacillus subtilis
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-2095
RX   DOI; 10.1016/0378-1119(88)90476-3
RX   PUBMED; 2840350.
RA   Iwakura M., Kawata M., Tsuda K., Tanaka T.;
RT   "Nucleotide sequence of the thymidylate synthase B and dihydrofolate
RT   reductase genes contained in one Bacillus subtilis operon";
RL   Gene 64(1):9-20(1988).
XX
DR   GOA; P54173.
DR   InterPro; IPR007686; PgpA.
DR   PDB; 1TLQ; X-ray.
DR   UniProtKB/Swiss-Prot; P54172; YPJP_BACSU.
XX
CC   Draft entry and computer readable copy of sequence [1] kindly
CC   submitted by M.Iwakura 09-AUG-1988.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2095
FT                   /organism="Bacillus subtilis"
FT                   /strain="W168"
FT                   /sub_strain="PY79"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1423"
FT   CDS             798..1592
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="thymidylate synthase B (thyB)"
FT                   /db_xref="GOA:P11044"
FT                   /db_xref="HSSP:1TYS"
FT                   /db_xref="InterPro:IPR000398"
FT                   /db_xref="UniProtKB/Swiss-Prot:P11044"
FT                   /protein_id="AAA22852.1"
FT                   /translation="MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPML
FT                   TTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGA
FT                   DGETIDQISRLIEDIKTNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLSCQL
FT                   YQRSADVFLGVPFNIASYALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLER
FT                   DVRPLPQLRFARKVDSIFNFAFEDFIIEDYDPHPHIKGAVSV"
FT   CDS             1589..2095
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="dihydrofolate reductase (dfrA)"
FT                   /db_xref="GOA:P11045"
FT                   /db_xref="HSSP:1DHI"
FT                   /db_xref="InterPro:IPR017925"
FT                   /db_xref="UniProtKB/Swiss-Prot:P11045"
FT                   /protein_id="AAA22853.1"
FT                   /translation="MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRK
FT                   TFESIGRPLPNRKNIVVTSAPDSEFQGCTVVSSLKDVLDICSGPEECFVIGGAQLYTDL
FT                   FPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKKNSS
FT                   KVGGF"
XX
SQ   Sequence 2095 BP; 622 A; 412 C; 483 G; 578 T; 0 other;
     gatcttctca gattccatgt gcgaagagac cacccgcctc atgacggcta ctggtttaat        60
     tttcattatc atacagcaga agatggattt caatcacatc atgagcttgg gagcatttac       120
     tgggaccgca acacgcctcc caactggatg agtgcctaaa ggagatgcca ttagacatga       180
     aaaagtacac aatgaatgaa atggtcgata taacaaagga tatgctgaac aaacggggcg       240
     ttatgataga agatattgca cggattgttc aaaaacttca ggaaaaatac aatccaaatc       300
     tgccgctcag tgtttgcatg gagaatgtag aaaaagtcct gaacaaacgt gaaattattc       360
     atgctgtttt gacaggcctt gcactcgatc agcttgcaga acagaaacgt tctccccgaa       420
     ccgctgcagc accttgttga aacggatgaa ccgctttacg gcatagatga aattatcccg       480
     ctttcaatcg ttaatgtgta cgggtcgatc ggtttgacca atttcggtta tttggataaa       540
     gagaagattg gaattattaa ggaacttgac tgaaagtcca gacggtactt cacacccttt       600
     ttggatgata ttgtggcagc tcttgctgca gcagcggcga gcagaattgc acatacgcat       660
     caggatctgc aagatgaaga aaaagaacag gatgaaaagc ctgtcgtcag ctgactataa       720
     aaaaatcatt tctgggttca aaaatgattt tttattgtgt tacactacta gaagactact       780
     tttaaaggat gaaaaaaatg aaacagtata aggatttctg cagacatgtt ttagagcatg       840
     gtgagaaaaa gggagaccgg actgggaccg gaacaatcag cactttcgga tatcaaatga       900
     gatttaattt acgggaaggc tttccgatgc tcaccactaa aaaactccac tttaaatcaa       960
     ttgcgcatga actgctgtgg ttcttaaaag gagatacgaa tgtacgctat ctgcaggaaa      1020
     acggagtgcg aatctggaat gagtgggctg atgaaaacgg tgaacttgga cctgtatatg      1080
     gctcccaatg gcgttcttgg cggggagctg atggagaaac cattgatcaa atttcccgtc      1140
     ttattgaaga tattaaaaca aatccgaact ccagacgctt aatcgtcagc gcctggaatg      1200
     ttggtgaaat tgataaaatg gcgttgccgc cgtgccattg cctgttccaa ttctatgtgt      1260
     ctgacggcaa gctgtcctgt cagctgtatc agcgctctgc cgatgttttc ttaggtgtgc      1320
     cgtttaatat tgcatcttat gccctcctaa ccatgatcat tgctcatgtg actgggcttg      1380
     aaccgggcga gttcatccat acgtttggtg atgttcatat ttaccaaaat catattgaac      1440
     aagtcaattt gcagctggaa agagatgtta gaccgcttcc gcagcttcgt ttcgccagaa      1500
     aggttgattc tatttttaac tttgcatttg aggactttat catcgaggat tatgatccgc      1560
     atcctcatat aaaaggggcg gtcagcgtat gatttcattc atttttgcga tggatgccaa      1620
     caggcttatc ggcaaagaca atgatttgcc gtggcatttg cccaatgatc ttgcatactt      1680
     taagaaaata acatcgggcc attcaatcat tatgggccgg aaaacatttg aatcgatcgg      1740
     acgtccgctt ccaaatcgga aaaatattgt cgttacctca gcgccggatt cagaatttca      1800
     gggatgcacg gttgtcagtt cattaaagga tgtactggac atttgttcag gccctgaaga      1860
     atgctttgtg atcggagggg ctcagctcta tacggacctg ttcccttatg cggacagact      1920
     gtatatgacg aaaattcatc acgagtttga gggtgaccgt cactttcctg aatttgatga      1980
     atccaattgg aagctggttt cttctgagca ggggaccaaa gacgaaaaaa acccgtatga      2040
     ttacgaattt ctaatgtatg aaaaaaagaa ttcttctaaa gtgggaggat tttaa           2095
//
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