spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M19485; SV 1; linear; genomic DNA; STD; PRO; 1622 BP.
XX
AC   M19485;
XX
DT   20-FEB-1989 (Rel. 18, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 3)
XX
DE   B.stearothermophilus malic acid gene, complete cds.
XX
KW   (S)-malate:NAD+ oxireductase; malic enzyme.
XX
OS   Geobacillus stearothermophilus
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
XX
RN   [1]
RP   1-1622
RX   PUBMED; 2644282.
RA   Kobayashi K., Doi S., Negoro S., Urabe I., Okada H.;
RT   "Structure and properties of malic enzyme from Bacillus
RT   stearothermophilus";
RL   J. Biol. Chem. 264(6):3200-3205(1989).
XX
CC   Draft entry and computer_readable sequence for [1] kindly submitted
CC   by I.Urabe, 06-OCT-1988.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1622
FT                   /organism="Geobacillus stearothermophilus"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1422"
FT   -35_signal      29..34
FT   -10_signal      52..57
FT   RBS             119..166
FT                   /note="ribosome binding site"
FT   CDS             119..1555
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="malic enzyme (EC 1.1.1.38)"
FT                   /db_xref="GOA:P16468"
FT                   /db_xref="InterPro:IPR002912"
FT                   /db_xref="InterPro:IPR012301"
FT                   /db_xref="InterPro:IPR012302"
FT                   /db_xref="InterPro:IPR015884"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P16468"
FT                   /protein_id="AAA22585.1"
FT                   /translation="MALPGGAAMNITIRLQFEKDIVSFSDIAAAIGKAGGDIVGIDVIS
FT                   SSKVHTVRDITVSALDTKQCDLIIEALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPV
FT                   KTRDDLSRVYTPGVARVCTAIAEDPRKAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAM
FT                   PVMEGKAMLFKEFAGVDAFPICLDTKDTEEIIQIVKAIAPAFGGINLEDISAPRCFEIE
FT                   KRLKEELDIPVFHDDQHGTAVVLLAGLLNALKIVDKKLEDIKVVLTGIGAAGIACTKIL
FT                   LAAGVRNIIGVDRHGAIHRDETYENPYWQEYAQLTNPDNLKGSLSDVIAGADVFIGVSA
FT                   PGILKVEDVKKMARDPIVFAMANPIPEIDPELAEPYVRVMATGRSDYPNQINNVLCFPG
FT                   IFRGALDCRAREINEEMKLAAAKAIASVVTEDELNETYIIPSVFNSKVVERVRQAVVEA
FT                   AYRTGVARKDNIPVGGYTGQ"
XX
SQ   Sequence 1622 BP; 412 A; 385 C; 474 G; 351 T; 0 other;
     ctgcagaaag gggcatgtac aataagggtt gtctttttcc ggttcatagg ataacatttg        60
     taccgtaaga tgatttactg atttaaacaa aaatagagaa aaggaaggac gattggttat       120
     ggcattacca ggcggggcag ccatgaacat tacgattcgt ctccagttcg aaaaagatat       180
     cgtctcattc agcgatatcg ccgccgcgat tggcaaagcg ggcggggaca ttgtcgggat       240
     tgacgtcatt tcgtcaagca aagttcacac ggtgcgcgat attaccgtca gcgccctcga       300
     tacgaagcag tgcgacttga tcattgaggc gctgaaaaaa attcgcggcg tcaaaattgt       360
     gaacgtttcc gaccgcacgt ttttgatgca catcgggggg aaaattgaaa caaattcaaa       420
     aatcccagtg aaaacacgcg acgacttgtc gcgggtatac acgccgggcg tggcgcgcgt       480
     ctgcacggcg attgccgaag atccgcgaaa ggcgtattcg ctgacgatta aacggaatac       540
     ggtcgccgtt gtctcggacg gcacggcggt gcttgggctt ggcgacatcg gtccgtacgc       600
     ggcgatgcca gtcatggaag ggaaagcgat gctgtttaag gaatttgccg gagtggacgc       660
     gttcccgatt tgtttggata cgaaagacac ggaagaaatc attcaaattg tgaaagcgat       720
     cgccccggcg tttggcggca ttaacctgga ggacatttcc gccccacgtt gttttgaaat       780
     tgaaaagcgg ctgaaagaag agctggatat tcccgtgttt catgatgacc agcatggcac       840
     ggcggtcgtg ctcttggcgg gactgcttaa cgcgctgaaa atcgttgaca aaaagctcga       900
     agacattaaa gtcgtcttaa ccggcatcgg tgcggccggc atcgcctgca cgaaaatttt       960
     gcttgcggcc ggcgtgcgca acattatcgg ggtcgaccgc catggcgcca tccaccgcga      1020
     tgaaacgtac gaaaatccgt actggcaaga gtatgcgcaa ctcacgaacc cggataatct      1080
     gaaaggaagc ttgtccgatg tcatcgccgg cgcagatgta tttatcggcg tttcggctcc      1140
     gggcatttta aaagtggaag atgtgaaaaa aatggcgcgc gatccgatcg tgtttgcgat      1200
     ggccaacccg attccggaaa ttgatccgga gctggccgaa ccgtacgtgc gcgtcatggc      1260
     gaccgggcgt tccgactatc cgaaccaaat caacaacgtc ctttgcttcc cgggcatttt      1320
     ccgcggggcg cttgactgcc gggcgagaga aattaacgag gaaatgaagc tcgctgccgc      1380
     gaaggcgatt gcctctgtcg tgacggagga tgaattgaac gaaacataca tcatcccgag      1440
     cgtcttcaac agcaaagtcg tcgaacgcgt cagacaagcg gtcgtcgaag cggcttaccg      1500
     cactggggtg gcgcggaagg acaatatccc ggttggcgga tatacagggc agtaaagcaa      1560
     ggaatgcgat ggaaccgacg gacggcttcc tttatagggg aagccgtttt ttttggctgc      1620
     ag                                                                     1622
//
spacer
spacer