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EBI DbfetchID M18279; SV 1; linear; genomic DNA; STD; PRO; 2594 BP. XX AC M18279; XX DT 16-JUL-1988 (Rel. 16, Created) DT 06-JUL-1999 (Rel. 60, Last updated, Version 4) XX DE Pseudomonas sp. cephalosporin acylase (acyI) gene, complete cds. XX KW cephalosporin acylase. XX OS Pseudomonas sp. OC Bacteria; Proteobacteria. XX RN [1] RP 1-2594 RX PUBMED; 3680178. RA Matsuda A., Toma K., Komatsu K.; RT "Nucleotide sequences of the genes for two distinct cephalosporin acylases RT from a Pseudomonas strain"; RL J. Bacteriol. 169(12):5821-5826(1987). XX FH Key Location/Qualifiers FH FT source 1..2594 FT /organism="Pseudomonas sp." FT /strain="SE83" FT /mol_type="genomic DNA" FT /clone="pACGX8." FT /db_xref="taxon:306" FT CDS 135..815 FT /codon_start=1 FT /transl_table=11 FT /product="unknown protein" FT /note="ORF1; putative" FT /db_xref="InterPro:IPR005493" FT /db_xref="UniProtKB/TrEMBL:Q52425" FT /protein_id="AAA88423.1" FT /translation="MPDYRIEAMPEQLPAALVEKLQKVETATIGHSQHWGFMDRRIQPL FT LRGKRIAAAAVTLAIPGQDSTLLHHTLGLLRPGDILVVDRLGDDKHACWGGGVTVAAKA FT AGAVGGIVDGPCTDLTEIEDSDFPMWCRGMSPITTRLYNLGGTLNLPISCGNVPVKAGD FT VILADESGVLVLPRAEAEAIADAAIARQERGERSQGRVKAGEKLGDISGATKMVLDAIA FT AAKG" FT CDS 827..2503 FT /codon_start=1 FT /transl_table=11 FT /product="cephalosporin acylase" FT /note="precursor" FT /db_xref="GOA:P15557" FT /db_xref="InterPro:IPR000101" FT /db_xref="UniProtKB/Swiss-Prot:P15557" FT /protein_id="AAA88424.1" FT /translation="MNAPVPVPRVADFTCEKKPASGSRGMVVTNHPLASAAGAQILLAG FT GNAIDAAVASLFALTVAEPMMVGILGGGLSHIRLADGRHVVIDNLSTAPGKATAEMYEC FT LSDEIGKQRDTRDRQNVVGAKAVAVPGALKGWCEALARFGTLPLAEVLQPAIGLAERGF FT VVTPYLSNCITDNAGDLARDPGLAAMLLPGGKPLQPGMRLVQSDYAASLKLIAAEGPDA FT LYGGKLGRALTDYMAANGGLIDQADLANYRIELREPIRGSYRGYEIIGPPPPSSSGVHI FT TQMLNILEGYDIGSLGFGSTDAVHLLAEALKIAFADRAVATADPAFVKVPVARLIDKAY FT ADERRALIEMEQAKSWTAGLSGGESADTTHVTVADAMGNVVSATQTINGLFGACVQIPG FT TGMIANNYMYNFDPHPGRALSIAPGKRVFTSMAPMMALKEGRIAFALGLPGALRIFPSA FT LQAIVNLIDHRMSLQEAVEAPRVWTEGGVLELEEAIPEAVAQALIARGHKVVRSPRVAG FT GMNAIAFNPDGTLTGAACWRADGTPVAISGGLARAGARFTI" FT mat_peptide 830..1927 FT /product="cephalosporin acylase large subunit" FT /note="putative" FT mat_peptide 1928..2500 FT /product="cephalosporin acylase small subunit" FT /note="putative" XX SQ Sequence 2594 BP; 399 A; 922 C; 857 G; 416 T; 0 other; gatccgtcga agggcgcacc gatccgctca tcgcggccct gtccgtggtg ctcatcgtcg 60 cgacgctcgc cgtcgttctc gtggtcgaac gcctggtcgg cgtcgcccgt gccgttggaa 120 agtaggagga tctcatgccc gattatcgca tcgaagccat gcccgagcag ctgccggctg 180 cgctggtcga gaagctgcag aaggtcgaga ccgcgaccat cggccattcc cagcactggg 240 gcttcatgga tcgccgcatc cagccgctgc tgcgcggcaa gcgcatcgcc gcggcggccg 300 tgacgctggc gatccccggc caggattcga ccctgctgca ccacacgctc ggcctgctgc 360 gtcccggcga catcctcgtc gtcgaccgtc tcggcgacga caagcacgct tgctggggcg 420 gtggcgtcac cgtcgcggcg aaggcggccg gcgcggtcgg cggcatcgtc gacggtccct 480 gcaccgacct caccgagatc gaggattccg atttcccgat gtggtgccgc ggcatgtcgc 540 cgatcaccac gcggctctac aatctcggcg gcacgctcaa tctgccgatc tcctgcggca 600 atgtcccggt caaggccggc gacgtgatcc tcgccgatga atccggcgtg ctggtgctgc 660 cccgcgccga ggccgaggcg atcgcggacg ccgccatcgc ccggcaggag cgcggcgagc 720 gcagccaggg ccgggtgaag gcgggcgaga agctcggcga catctccggc gcgaccaaga 780 tggtgctcga cgccatcgcc gcagcgaagg gctgacgagg ccgatcatga acgctcccgt 840 ccccgtcccg cgcgtcgccg atttcacctg cgagaagaag cctgcgagcg gctcgcgcgg 900 catggtcgtc accaaccacc cgctcgcctc ggcagccggc gcgcagatcc tgctcgccgg 960 cggcaatgcc atcgacgcgg ctgtcgcgtc gctcttcgcg ctgacggtgg ccgagccgat 1020 gatggtcggc atcctcggcg ggggcctgag ccatatccgg ctcgccgacg ggcgtcatgt 1080 cgtgatcgac aatctctcga ccgcgccggg caaggcgacg gccgagatgt acgagtgcct 1140 gtccgacgag atcggcaagc agcgcgacac gcgcgaccgc cagaacgtgg tcggagccaa 1200 ggcggtcgcg gttcccggcg cgctcaaggg ctggtgcgag gcgctcgccc gcttcggcac 1260 gctgccgctc gccgaggttc tccagccggc gatcgggctg gcggagcgcg gcttcgtggt 1320 cacgccctat ctctcgaact gcatcaccga caacgcgggc gatctcgccc gcgaccccgg 1380 cctcgcggcg atgctgctgc cgggcggaaa gccgctccag ccgggcatgc ggctcgtcca 1440 gtccgactat gccgcgagcc tcaaactgat cgcggctgag gggccggacg cgctctatgg 1500 cggcaagctc ggccgggcgc tgaccgatta catggcggcc aatggcggcc tgatcgatca 1560 ggccgacctc gccaattacc gcatcgaact gcgcgagccg attcgcggct cctatcgcgg 1620 ctacgagatc atcggcccgc cgccgccctc gtcatcgggc gtgcatatca cgcagatgct 1680 caacattctc gaaggctatg atatcggctc gctcggcttc ggctcgacgg acgctgtgca 1740 tctgctcgcc gaagccctga agatcgcctt cgccgaccgc gccgtggcga cagccgatcc 1800 ggccttcgtc aaggttccgg tcgcgcgatt gatcgacaag gcctatgccg acgagcgccg 1860 cgcgctcatt gagatggagc aggcgaagag ctggacggcc gggctctctg gcggcgaatc 1920 cgccgacacc actcatgtca ccgtcgctga cgccatgggg aatgtcgtca gcgcgacgca 1980 gacgatcaac gggctgttcg gcgcctgcgt gcagattccg ggcaccggca tgatcgccaa 2040 caactacatg tacaacttcg atccgcatcc cggccgggcg ctctcgatcg cgccgggaaa 2100 gagggtcttc acctcgatgg cgccgatgat ggcgttgaag gagggacgga tcgcctttgc 2160 gctcggcttg cctggcgcgc tccgcatctt cccctcggcg ctgcaggcga tcgtcaacct 2220 gatcgaccac cgcatgagcc tgcaggaggc ggtcgaggcg ccacgcgtct ggacggaggg 2280 cggcgtgctc gaactcgagg aagcgatccc cgaggccgtg gcacaagcgc tgatcgcgcg 2340 cggccataag gtggtgcgct cgccccgcgt ggccggtggc atgaacgcca tcgccttcaa 2400 tccggacggt accttgaccg gtgccgcctg ctggcgcgcc gacggcacac ccgtcgccat 2460 ctccggcggg ctcgcccgtg ccggtgcccg cttcaccatc tgacccgttt ttttgactgg 2520 accatcccat gcccgagacc atctgcctcc tcgacatgac ctcgcccgag cgcgccgatc 2580 gcttgcgaga attc 2594 // |
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