EBI Dbfetch

ID   M16625; SV 1; linear; genomic DNA; STD; PHG; 1591 BP.
XX
AC   M16625;
XX
DT   02-APR-1988 (Rel. 15, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 5)
XX
DE   Bacteriophage H19B (from E.coli) sltIA and sltIB genes encoding Shiga-like
DE   toxin I subunits A and B, complete cds.
XX
KW   Shiga-like toxin; sltA gene; sltB gene.
XX
OS   Enterobacteria phage H-19B
OC   Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae;
OC   Lambda-like viruses; unclassified Lambda-like viruses.
XX
RN   [1]
RP   1-1591
RX   DOI; 10.1073/pnas.84.13.4364
RX   PUBMED; 3299365.
RA   Calderwood S.B., Auclair F., Donohue-Rolfe A., Keusch G.T., Mekalanos J.J.;
RT   "Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli";
RL   Proc. Natl. Acad. Sci. U.S.A. 84(13):4364-4368(1987).
XX
CC   Draft entry and computer-readable sequence for [1] kindly provided
CC   by S.B.Calderwood, 07-AUG-1987. A dyad symmetry located at
CC   positions 203-223 may be involved in regulation of SLT production
CC   by iron. Ribosome binding sites are found at positions 316-320 and
CC   1278-1281. NCBI gi: 215043
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1591
FT                   /organism="Enterobacteria phage H-19B"
FT                   /host="H19"
FT                   /mol_type="genomic DNA"
FT                   /clone="pSC[2,4]."
FT                   /db_xref="taxon:69932"
FT   sig_peptide     332..397
FT                   /codon_start=1
FT                   /gene="sltIA"
FT   CDS             332..1279
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="sltIA"
FT                   /product="Shiga-like toxin I subunit A"
FT                   /note="precursor;  NCBI gi: 215044"
FT                   /db_xref="GOA:P08026"
FT                   /db_xref="InterPro:IPR016331"
FT                   /db_xref="UniProtKB/Swiss-Prot:P08026"
FT                   /protein_id="AAA98099.1"
FT                   /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRSA
FT                   IGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTGFV
FT                   NRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTSYLD
FT                   LMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAEDVDLT
FT                   LNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMASDEFPS
FT                   MCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
FT   mat_peptide     398..1276
FT                   /codon_start=1
FT                   /gene="sltIA"
FT                   /product="Shiga-like toxin I subunit A"
FT   sig_peptide     1289..1348
FT                   /codon_start=1
FT                   /gene="sltIB"
FT   CDS             1289..1558
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="sltIB"
FT                   /product="Shiga-like toxin I subunit B"
FT                   /note="precursor;  NCBI gi: 215045"
FT                   /db_xref="GOA:P69179"
FT                   /db_xref="InterPro:IPR003189"
FT                   /db_xref="PDB:1BOS"
FT                   /db_xref="UniProtKB/Swiss-Prot:P69179"
FT                   /protein_id="AAA98100.1"
FT                   /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKVG
FT                   DKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
FT   mat_peptide     1349..1555
FT                   /codon_start=1
FT                   /gene="sltIB"
FT                   /product="Shiga-like toxin I subunit B"
XX
SQ   Sequence 1591 BP; 424 A; 272 C; 383 G; 512 T; 0 other;
     aatattgtgt attttttgta ttgcaggatg accctgtaac gaagtttgcg taacagcatt        60
     ttgctctacg agtttgccag cctcccccag tggctggctt ttttatgtcc gtaacatcct       120
     gtgtatcaat aaatgttgtt atctacgtac gtcaagtagt cgcatgagat ctgaccagat       180
     atgttaaggt tgcagctctc tttgaatatg attatcattt tcattacgtt attgttacgt       240
     ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg aggatgtcaa gaatatagtt       300
     atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta gagtgctaac       360
     ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct tagacttctc       420
     gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag gtactccatt       480
     acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggct caggggataa       540
     tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta ataatctacg       600
     gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga caaataatgt       660
     tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag cggttacatt       720
     gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc gtacggggat       780
     gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc atagtggaac       840
     ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga cagctgaagc       900
     tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc tcagtgggcg       960
     ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa ggttgagtag      1020
     cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt cttttggaag      1080
     cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc atgcatcgcg      1140
     agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg gaagagtccg      1200
     tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa ttctgatgcg      1260
     cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag ctgcatcgct      1320
     ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa aggtggagta      1380
     tacaaaatat aatgatgacg atacctttac agttaaagtg ggtgataaag aattatttac      1440
     caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga tgactgtaac      1500
     cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt ttcgttgact      1560
     cagaatagct cagtgaaaat agcaggcgga g                                     1591
//