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EBI DbfetchID M16187; SV 1; linear; genomic DNA; STD; PRO; 4140 BP. XX AC M16187; XX DT 02-APR-1988 (Rel. 15, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE K.aerogenes pullulanase (pulA) gene, complete cds. XX KW pullulanase. XX OS Klebsiella aerogenes OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. XX RN [1] RP 1-4140 RX PUBMED; 3155373. RA Katsuragi N., Takizawa N., Murooka Y.; RT "Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes RT W70"; RL J. Bacteriol. 169(5):2301-2306(1987). XX CC Draft entry and computer-readable copy of sequence in [1] kindly CC provided by Y.Murooka, 23-JUN-1987. XX FH Key Location/Qualifiers FH FT source 1..4140 FT /organism="Klebsiella aerogenes" FT /strain="W70" FT /mol_type="genomic DNA" FT /db_xref="taxon:28451" FT sig_peptide 330..386 FT /note="pullulanase signal peptide" FT CDS 330..3638 FT /codon_start=1 FT /transl_table=11 FT /EC_number="3.2.1.41" FT /note="pullulanase precursor" FT /db_xref="GOA:P07811" FT /db_xref="HSSP:1M7X" FT /db_xref="InterPro:IPR011839" FT /db_xref="UniProtKB/Swiss-Prot:P07811" FT /protein_id="AAA25124.1" FT /translation="MLRYTCHALFLGSLVLLSGCDNSSSSSTSGSPGSPGNPGNPGTPG FT TPDPQDVVVRLPDVAVPGEAVQASARQAVIHLVDIAGITSSTPADYATKNLYLWNNETC FT DALSAPVADWNDVSTTPTGSDKYGPYWVIPLTKESGCINVIVRDGTNKLIDSDLRVSFS FT DFTDRTVSVIAGNSAVYDSRADAFRAAFGVALADAHWVDKTTLLWPGGENKPIVRLYYS FT HSSKVAADSNGEFSDKYVKLTPTTVNQQVSMRFPHLASYPAFKLPDDVNVDELLQGETV FT AIAAESDGILSSATQVQTAGVLDDTYAAAAEALSYGAQLTDSGVTFRVWAPTAQQVELV FT IYSADKKVIASHPMTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPY FT AHSLSTNSEYSQVVDLNDSALKPEGWDGLTMPHAQKTKADLAKMTIHESHIRDLSAWDQ FT TVPAELRGKYLALTAQESNMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQP FT FSRLCEVNSAVKSSEFAGYCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNW FT GYDPFHYTVPEGSYATDPEGTARIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDR FT TSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFR FT FDLMGYHPKAQILSAWERIKALNPDIYFFGEGWDSNQSDRFEIASQINLKGTGIGTFSD FT RLRDAVRGGGPFDSGDALRQNQGVGSGAGVLPNELTTLSDDQARHLADLTRLGMAGNLA FT DFVLIDKDGAVKRGSEIDYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAAQEADL FT DTRVRMQAVSLATVMLGQGIAFDQQGSELVRSKSFTRDSYDSGDWFNRVDYSLQDNNYN FT VGMPRSSDDGSNYDIIARVKDAVATPGETELKQMTAFYQELTALRKSSPLFTLGDGATV FT MKRVDFRNTGADQQTGLLVMTIDDGMQAGASLDSRVDGIVVAINAAPESRTLQDFAGTS FT LQLSAIQQAAGDRSLASGVQVAADGSVTLPAWSVAVLELPQGESQGAGLPVSSK" FT mat_peptide 387..3617 FT /note="pullulanase mature peptide" XX SQ Sequence 4140 BP; 881 A; 1186 C; 1226 G; 847 T; 0 other; aaccccatca cagaaactgg cattttaatt cctttgctaa tttatttttc tgcacgttgg 60 cgtaataccc taccgaaaca ggtaggtgat tattggcttt tgaattattt attgccggag 120 agtggcgata agcgtggcga atcataatca gtttgcggtt tcgggtcgtc attcccttta 180 ttgatttact cccccggctt cctacgcccc ccgctcattt gtgggggatg attgcgcctg 240 ggaaagcaaa aatatctaat tagcgcgctg taaagatatt catcctttct taccttttat 300 aatttaataa gcatattagg gactatcgaa tgctcagata tacctgtcat gccctatttc 360 ttggatcgtt agtattattg agtggctgtg ataacagctc ttcctcttct acctctggct 420 caccgggttc accaggcaat cctggcaacc caggcactcc cggcacgccc gacccgcagg 480 atgtcgtcgt ccgcttaccg gacgttgccg tcccaggcga agcggtgcag gcttccgcca 540 ggcaggctgt cattcatctc gtcgatatcg ccggcatcac cagcagcacg ccggccgact 600 atgcgacgaa aaacctctat ttatggaaca acgaaacctg tgacgcgctg agcgcgccgg 660 tggcggactg gaatgatgtc agcaccacgc cgaccggcag cgacaaatat ggcccttact 720 gggtgatccc gctgactaaa gagagcggat gcatcaacgt tatcgtccgc gatggcacca 780 ataagcttat cgacagcgac ctgcgcgtct ctttcagtga tttcaccgat cggacggtat 840 cggtcatcgc cggcaacagc gcggtctatg actcccgcgc cgacgccttc cgcgccgcct 900 ttggcgtggc gctggccgat gcgcactggg tcgataagac tactctgctg tggccgggtg 960 gggaaaataa acccattgtg cgcctctatt acagccacag cagtaaggtg gccgccgaca 1020 gtaacggcga atttagcgat aaatatgtca agctgacccc caccaccgtc aaccagcagg 1080 taagcatgcg cttcccgcat ctcgccagct atccagcctt taaactgccg gatgatgtta 1140 acgtcgatga attgctgcag ggcgagacgg tggcgatagc cgcggaaagc gatgggatcc 1200 tgagctcagc cacccaggtg cagaccgccg gcgtgctgga cgatacctat gccgccgccg 1260 ccgaggcgct gagctatggc gcccagctaa ccgatagcgg cgtgaccttc cgcgtctggg 1320 cgcccacggc gcagcaggtc gagctggtga tctatagcgc ggacaagaaa gtgatagcca 1380 gtcacccgat gacccgcgac agcgcctccg gcgcctggtc ctggcagggg ggaagcgacc 1440 tgaagggcgc cttctaccgc tacgcgatga cggtctacca cccgcagtcg cgtaaagtcg 1500 agcagtacga agtgaccgat ccctacgccc acagtttgtc gaccaactcg gagtacagcc 1560 aggtggtcga tctcaacgac agcgcgctga agccggaagg ctgggacggg ctgacgatgc 1620 cgcacgcgca gaaaaccaaa gccgatctgg cgaaaatgac gattcacgaa tcgcatattc 1680 gcgatctctc cgcctgggat caaaccgtgc ctgcggagct gcgcggtaag tatctggcgc 1740 tcaccgccca ggaaagcaat atggtccagc atctgaaaca gctgtcggcc tcgggcgtga 1800 cccatattga gctgctgccg gtcttcgatc tggcgacggt caatgagttc agcgacaagg 1860 tcgccgatat tcagcagccg ttcagccgcc tgtgcgaggt caatagcgcg gtgaagagca 1920 gcgagtttgc gggctattgc gacagcggtt cgacggttga agaggtgctg actcagctga 1980 agcagaacga cagcaaggat aacccgcagg tgcaggcgtt gaatacgctg gtggcgcaga 2040 ccgactccta taactggggc tacgatccgt tccactacac ggtaccggaa ggatcctacg 2100 ccaccgatcc ggaaggcaca gcgcgtatta aagagttccg caccatgatt caggcgatca 2160 agcaggatct gggaatgaac gtcattatgg acgtggtgta caaccacacc aacgccgccg 2220 gcccgaccga tcgcacctcg gtactggata agatcgtccc ctggtactac cagcgtctga 2280 acgaaaccac cggcagcgtg gaatcggcta cctgttgctc cgactcggcg ccagagcacc 2340 ggatgttcgc caagcttatc gccgattcac tggcggtatg gaccaccgat tataagatcg 2400 atggcttccg cttcgacctg atgggctacc acccgaaagc gcagatcctc tcggcctggg 2460 aacgcattaa agcgctgaac ccggatatct acttctttgg cgaaggctgg gattccaacc 2520 agagcgatcg ctttgaaatt gcctcgcaaa tcaatctcaa aggcaccggg atcggcacgt 2580 tctccgatcg tctgcgcgac gccgtgcgcg gcggcgggcc gttcgactcc ggtgacgcat 2640 tacgccagaa ccagggcgtg ggcagcgggg ctggcgttct gccgaatgag ctgaccaccc 2700 tgagcgacga tcaggcgcgt cacctcgccg atctgacccg tctcggcatg gccggtaacc 2760 ttgcggactt tgtgctgatc gacaaagacg gcgcggtgaa gagaggcagc gagattgatt 2820 ataacggcgc gccaggcggc tatgcggctg atccgacgga agtcgtgaac tatgtgtcaa 2880 aacacgataa ccagacgctg tgggacatga tcagctataa agccgctcag gaggcggatc 2940 tcgatacccg cgtccggatg caggcggtgt cgctggcgac ggtgatgctc ggccagggga 3000 tcgcctttga ccagcagggc tcggagctcg tgcgctctaa atcctttacc cgcgattcgt 3060 atgattccgg cgactggttt aaccgcgtgg actactccct gcaggacaac aactacaacg 3120 tcggtatgcc gcgcagcagc gatgatggca gcaattatga cattatcgcc cgggtgaaag 3180 acgcggtggc tactccgggt gaaacggagc tcaagcagat gaccgcgttt tatcaggagc 3240 tgaccgcgct gcgtaaatcg tctccgctgt ttaccctcgg cgacggcgcg acggtgatga 3300 agcgcgtgga cttccgcaat actggcgccg atcagcagac gggtctgctg gtgatgacca 3360 tcgatgacgg gatgcaggct ggcgccagtc tggacagccg tgtcgacggc atcgtggtgg 3420 cgatcaacgc cgcgccggaa agccggacgc tgcaggactt cgccggcaca tcgctccagc 3480 tgagcgctat tcagcaggcg gcgggcgacc ggtcgctggc gagcggcgtg caggttgccg 3540 ctgacggttc ggtcacgctg ccggcctggt cggttgccgt tctcgagttg ccgcagggcg 3600 agtcgcaggg cgctggcctg ccggtgagca gtaaataaca taacgtaggg cgcccgtaag 3660 cgtctggctg atgacaaacc tgtttcgtta ttgtgctcgc ctttctgtcg ggtggcggct 3720 ttcgcttacc cggcctacga aagattgcaa tttcaaacag ttatgcagtt atctgtaggc 3780 ccgcgcaagt agcgcgccgg ccacttcctg aagattgcac tttgtcagca gtctggggca 3840 tccgtaaggg cgccttctcg ttatcgtgag ggaacaggag acggtggatg tcggataagt 3900 gcatgaaaca gtatgcagta tctgtagccg ccaacggtac gccgccggga cttcctgaag 3960 attgcacttt gtcagcagtc tggggcatcc gtaagggcgc cttctcgtta tcgtgaggga 4020 acaggagacg gattgagtat gattgttcgt gatgacagtg aaaagcatcc gcctctggtc 4080 gcatcagccg ccgtcctcgc tggatgatcg ccggtgagcc gcggtcggat ggtgcagatc 4140 // |
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