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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M15674; SV 1; linear; genomic DNA; STD; PRO; 1310 BP.
XX
AC   M15674;
XX
DT   02-APR-1988 (Rel. 15, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Bacillus amyloliquefaciens beta-glucanase (bglA) gene, complete cds.
XX
KW   beta-glucanase; glucanase.
XX
OS   Bacillus amyloliquefaciens
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-1310
RX   DOI; 10.1016/0378-1119(86)90278-7
RX   PUBMED; 3106158.
RA   Hofemeister J., Kurtz A., Borriss R., Knowles J.;
RT   "The beta-glucanase gene from Bacillus amyloliquefaciens shows extensive
RT   homology with that of Bacillus subtilis";
RL   Gene 49(2):177-187(1986).
XX
CC   Draft entry and clean copy of sequence in [1] kindly provided by
CC   J.Hofemeister, 16-JUN-1987.
CC   There are two putative promoters.  The first is located at
CC   positions 393 to 398 (-35 region) and 417 to 422 (-10 region).  The
CC   second is located at positions 410 to 415 (-35 region) and 435 to
CC   440 (-10 region).  There is a putative ribosome binding site at
CC   positions 451 to 458.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1310
FT                   /organism="Bacillus amyloliquefaciens"
FT                   /mol_type="genomic DNA"
FT                   /clone="pEG1"
FT                   /tissue_lib="ATCC 15841/BE20/78"
FT                   /db_xref="taxon:1390"
FT   CDS             <1..258
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="open reading frame; putative"
FT                   /db_xref="GOA:Q44651"
FT                   /db_xref="HSSP:1H99"
FT                   /db_xref="InterPro:IPR011608"
FT                   /db_xref="UniProtKB/TrEMBL:Q44651"
FT                   /protein_id="AAA87322.1"
FT                   /translation="EFNEESLHYYRFVTHLKFFDQRLFNGTHMESQDDFLLETVKEKYH
FT                   QAYKCTKNIHTYIEKEYGHKLTSDELLYLTIHIERVVKQV"
FT   sig_peptide     469..543
FT                   /product="beta-glucanase"
FT   CDS             469..1188
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="beta-glucanase"
FT                   /EC_number="3.2.1.73"
FT                   /db_xref="GOA:P07980"
FT                   /db_xref="HSSP:1GBG"
FT                   /db_xref="InterPro:IPR008263"
FT                   /db_xref="UniProtKB/Swiss-Prot:P07980"
FT                   /protein_id="AAA87323.1"
FT                   /translation="MKRVLLILVTGLFMSLCGITSSVSAQTGGSFFEPFNSYNSGLWQK
FT                   ADGYSNGDMFNCTWRANNVSMTSLGEMRLALTSPSYNKFDCGENRSVQTYGYGLYEVRM
FT                   KPAKNTGIVSSFFTYTGPTEGTPWDEIDIEFLGKDTTKVQFNYYTNGAGNHEKFADLGF
FT                   DAANAYHTYAFDWQPNSIKWYVDGQLKHTATTQIPAAPGKIMMNLWNGTGVDDWLGSYN
FT                   GVNPIYAHYDWMRYRKK"
FT   mat_peptide     544..1185
FT                   /product="beta-glucanase"
FT                   /EC_number="3.2.1.73"
XX
SQ   Sequence 1310 BP; 402 A; 242 C; 294 G; 372 T; 0 other;
     gaattcaacg aagaatcgct gcactattat cgattcgtca cccacttaaa gtttttcgac        60
     cagcgtcttt ttaacggcac acacatggaa agccaggacg attttttact ggagacagtg       120
     aaagaaaagt atcatcaggc gtataaatgc acgaagaata tccataccta cattgagaaa       180
     gagtatgggc ataagctcac cagtgacgag ctgctgtatt taacgattca catagaaagg       240
     gtagtcaaac aagtataatg aaagcgcttt cctcgtatta attgtttctt ccattcatat       300
     ataggattgt tacggataaa gcaggcaaaa cctatctgtc tgtgctgatg gtagtttagg       360
     tttgtatttt taacagaagg attatcatta tttcgaccga tgttcccttt gaaaaggatc       420
     atgtatgatc aataaagaaa gcgtgttcaa aaaaggggga atgctaacat gaaacgagtg       480
     ttgctaattc ttgtcaccgg attgtttatg agtttgtgtg ggatcacttc tagtgtttcg       540
     gctcaaacag gcggatcgtt ttttgaacct tttaacagct ataactccgg gttatggcaa       600
     aaagctgatg gttactcaaa tggagatatg tttaactgca cttggcgtgc taataacgtc       660
     tctatgacgt cattaggtga aatgcgtttg gcgctgacaa gtccgtctta taacaagttt       720
     gactgcgggg aaaaccgctc ggttcaaaca tatggctatg gactttatga agtcagaatg       780
     aaaccggcta aaaacacagg gattgtttca tcgttcttca cttatacagg tccaacggag       840
     gggactcctt gggatgagat tgatatcgaa tttttgggaa aagacacaac gaaggttcaa       900
     tttaactatt atacaaatgg cgcaggaaac catgagaagt tcgcggatct cggatttgat       960
     gcagccaatg cctatcatac gtatgcgttc gattggcagc caaactctat taaatggtat      1020
     gtcgatgggc aattaaaaca tactgcaaca acccaaatac cggcagcgcc ggggaaaatc      1080
     atgatgaatt tgtggaatgg tacgggtgtt gatgattggc tcggttccta caatggcgta      1140
     aatccgatat acgctcatta cgactggatg cgctatagaa aaaaataatg tacagaaaag      1200
     gatttcgctg gcggaatcct tttttgatta aaacgaaata atcccaatca aaagcgccgc      1260
     aatcgtcatt acaatcgttg tccccacggc ccatttgatg gtgaattttt                 1310
//
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