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EBI DbfetchID M15333; SV 1; linear; genomic DNA; STD; PRO; 2040 BP. XX AC M15333; XX DT 19-SEP-1987 (Rel. 13, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE P.pseudoalcaligenes bphC gene encoding 2,3-dihydroxybiphenyl dioxygenase, DE complete cds, bphD gene encoding hydrolyzing enzyme, 5' end, and bphB gene DE encoding dihydrodiol dehydrogenase, 3' end. XX KW 2,3-dihydroxybiphenyl dioxygenase; dihydrodiol dehydrogenase; KW hydrolyzing enzyme. XX OS Pseudomonas pseudoalcaligenes OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-2040 RX PUBMED; 3793719. RA Furukawa K., Arimura N., Miyazaki T.; RT "Nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene of RT Pseudomonas pseudoalcaligenes"; RL J. Bacteriol. 169(1):427-429(1987). XX CC Draft entry and printed copy of sequence kindly provided by CC K.Furukawa, 04-AUG-1987. CC Potential ribosome binding sites for bphC and bphD are located at CC positions 937-943 and 1978-1984. No promoter-like sequence CC preceding the ribosome binding site of the bphC gene was found. CC Instead a long open reading frame (which may be the 3' terminal CC portion of the bphB gene) was extended more than 927 nucleotides. XX FH Key Location/Qualifiers FH FT source 1..2040 FT /organism="Pseudomonas pseudoalcaligenes" FT /mol_type="genomic DNA" FT /db_xref="taxon:330" FT CDS <1..927 FT /codon_start=1 FT /transl_table=11 FT /note="dihydrodiol dehydrogenase" FT /db_xref="GOA:P08694" FT /db_xref="InterPro:IPR002198" FT /db_xref="InterPro:IPR002347" FT /db_xref="InterPro:IPR016040" FT /db_xref="InterPro:IPR017711" FT /db_xref="UniProtKB/Swiss-Prot:P08694" FT /protein_id="AAA25752.1" FT /translation="CRTSARTCGIYSKPNRIDAHDRRIALTIKGIGKMKLKGEAVLITG FT GASGLGRALVDRFVAEAKVAVLDKSAERLAELETDLGDNVLGIVGDVRSLEDQKQAASR FT CVARFGKIDTLIPNAGIWDYSTALVDLPEESLDAAFDEVFHINVKGYIHAVKALPALVA FT SRGNVIFTISNAGFYPNGGGPLYTAAKQAIVGLVRELAFELAPYVRVNGVGPGGMNSDM FT RGPSSLGMGSKAISTVPLADMLKSVLPIGRMPEVEEYTGAYVFFATRGDAAPASGALVN FT YDGGLGVRGFFSGAGGNDLLEQLNIHP" FT CDS 949..1860 FT /codon_start=1 FT /transl_table=11 FT /note="2,3-dihydroxybiphenyl dioxygenase precursor" FT /db_xref="GOA:P08695" FT /db_xref="InterPro:IPR000486" FT /db_xref="InterPro:IPR004360" FT /db_xref="InterPro:IPR017626" FT /db_xref="UniProtKB/Swiss-Prot:P08695" FT /protein_id="AAA25753.1" FT /translation="MSIRSLGYMGFAVSDVAAWRSFLTQKLGLMEAGTTDNGDLFRIDS FT RAWRIAVQQGEVDDLAFAGYEVADAAGLAQMADKLKQAGIAVTTGDASLARRRGVTGLI FT TFADPFGLPLEIYYGASEVFEKPFLPGAAVSGFLTGEQGLGHFVRCVPDSDKALAFYTD FT VLGFQLSDVIDMKMGPDVTVPVYFLHCNERHHTLAIAAFPLPKRIHHFMLEVASLDDVG FT FAFDRVDADGLITSTLGRHTNDHMVSFYASTPSGVEVEYGWSARTVDRSWVVVRHDSPS FT MWGHKSVRDKALRATKHEQQPE" FT mat_peptide 952..1857 FT /note="2,3-dihydroxybiphenyl dioxygenase" FT CDS 1984..>2040 FT /codon_start=1 FT /transl_table=11 FT /note="hydrolyzing enzyme" FT /db_xref="UniProtKB/TrEMBL:Q52033" FT /protein_id="AAA25754.1" FT /translation="MLRAGLPGDQPGRVRALPA" XX SQ Sequence 2040 BP; 404 A; 570 C; 648 G; 418 T; 0 other; tgcaggacgt cggctcgaac ctgcgggatt tactcaaagc caaaccgaat tgatgcgcat 60 gaccggcgaa tcgctttaac aataaagggg attggaaaaa tgaaactgaa aggtgaagcg 120 gtactgatca cggggggcgc ctccggatta gggcgcgcgc tcgtggaccg attcgtggcc 180 gaagccaaag tggcggtgct cgacaagtcg gcagagcgcc ttgcagagct ggaaaccgat 240 ctgggcgaca acgtgctcgg catcgtcggc gatgtgcgtt cactggaaga ccagaaacag 300 gctgccagcc gctgcgtggc caggttcggg aaaatcgaca ccttgattcc caatgcaggc 360 atctgggatt actcgacggc cttggtcgac ctgcccgaag agagcctcga tgccgcgttc 420 gatgaggtct ttcacatcaa tgtcaagggt tatatccatg cagtgaaggc cctgccggcc 480 ctggtcgcca gccgtggcaa cgtgatcttc acgatctcca atgcgggctt ctatcccaat 540 ggcggcggcc ctctttacac cgcagccaag caggccatcg tgggcctggt gcgtgaactg 600 gcgttcgagc tggcgccata cgtgcgcgtc aacggcgtgg ggccgggcgg catgaatagc 660 gatatgagag gtccttcctc gctgggaatg ggcagcaagg cgatttcgac cgtaccgctg 720 gccgacatgc tgaagtcggt gctgccgatt ggtcgcatgc ccgaggtcga agagtacacc 780 ggggcctatg tgttttttgc cacccgaggc gatgcggcgc ctgcgtccgg ggccttggtg 840 aactacgacg gtggcttggg cgttcgtgga ttcttctcgg gtgccggagg taatgacttg 900 ctcgaacagc tgaatatcca tccctaagca tcgacgaagg agacagtaat gagcatcaga 960 agtttgggat acatggggtt tgcggtcagc gacgtagctg cttggcgttc gtttctgacg 1020 cagaaactgg gcttgatgga agcgggcacg accgacaacg gcgacctgtt ccgcatcgat 1080 tcgagagcct ggcggatcgc cgttcagcag ggcgaggttg acgatctggc ctttgccggc 1140 tacgaggtgg ccgatgcggc agggctggcg cagatggctg acaagctcaa acaggccggt 1200 atcgcagtca ccaccggcga tgcctcattg gccaggcgcc gcggggtgac cggattgatc 1260 acctttgccg acccgtttgg cctgccgttg gaaatttact atggcgccag cgaggtgttc 1320 gaaaaaccgt tcctgcctgg tgcggccgtg tcgggtttcc tgaccggcga gcaagggctg 1380 gggcatttcg tgcgctgcgt tccggattcg gacaaggcgc tggcgtttta taccgacgtg 1440 ctcggcttcc agttgtctga cgtcatcgac atgaaaatgg ggccggacgt gacggttcct 1500 gtgtacttcc tgcactgcaa cgaacgccac cacaccctgg caattgcggc attcccgctg 1560 ccaaaacgca ttcatcactt catgctcgaa gtcgcctcgc tcgatgacgt cggctttgca 1620 tttgatcggg ttgacgcgga cggcttgatc acctccacgc tggggcgcca caccaatgac 1680 cacatggtgt cgttctatgc ctcgaccccg tccggagtag aggtcgagta tggctggagt 1740 gcccgtaccg ttgaccgctc ctgggttgtg gtgaggcacg acagtccgag catgtggggc 1800 cacaagtctg tgcgcgacaa agcactgcgc gcaacaaagc atgaacaaca acctgaatag 1860 cacgggaatc cctgcatgaa actttactac agccctggtg cctgctccct gtcaccgcac 1920 attgcattgc gcgaggcggg cctgaacttc gagttggtgc aggtcgatct tgcgagcaag 1980 aagatgctcc gggcaggact acctggagat caacccggcc gggtacgtgc cttgcctgca 2040 // |
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