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EBI DbfetchID M14510; SV 1; linear; genomic DNA; STD; PRO; 2086 BP. XX AC M14510; XX DT 07-JUN-1987 (Rel. 12, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE E.chrysanthemi pectate lyase b (pelB) gene, complete cds. XX KW pectate lyase. XX OS Erwinia chrysanthemi OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Dickeya. XX RN [1] RP 1-2086 RX PUBMED; 3536853. RA Keen N.T., Tamaki S.; RT "Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and RT their high-level expression in Escherichia coli"; RL J. Bacteriol. 168(2):595-606(1986). XX CC Draft entry and computer-readable sequence of [1] kindly provided CC by N.T.Keen, 29-JAN-1987. XX FH Key Location/Qualifiers FH FT source 1..2086 FT /organism="Erwinia chrysanthemi" FT /mol_type="genomic DNA" FT /db_xref="taxon:556" FT sig_peptide 713..778 FT /note="pectate lyase B, signal peptide" FT CDS 713..1840 FT /codon_start=1 FT /transl_table=11 FT /note="pectate lyase B, preprotein (EC 4.2.2.2)" FT /db_xref="GOA:P04959" FT /db_xref="InterPro:IPR002022" FT /db_xref="InterPro:IPR011050" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P04959" FT /protein_id="AAA24847.1" FT /translation="MKSLITPIAAGLLLAFSQYSLAADTGGYTKTDGGDVSGAVKKTAS FT SMQDIVNIIEAAKVDANGKKVKGGAYPLVITYTGNEDSLINAAAANICGQWSKDARGVE FT IKDFTKGLTIIGANGSSANFGIWIVNSSDIVVRNMRIGYLPGGAQDGDMFRIDNSPNVW FT LDHNELFAANHECDGTKDGDTTFESAIDIKKGATYVTISYNYIHGVKKVGLSGFSSSDT FT AERNITYHHNIYSDVNARLPLQRGGNVHAYNNLYTGITSSGLNVRQNGKALIENNWFEN FT AVSPVTSRYDGSNFGTWVLKGNNITKPADFATYNITWTPDTKEYRNADTWTSTGTYPTV FT PYSYSPVSAQCVKDKLANYAGVGKNLATLASSACK" FT mat_peptide 779..1837 FT /note="pectate lyase B, mature peptide" XX SQ Sequence 2086 BP; 547 A; 620 C; 485 G; 431 T; 3 other; ggtacctggg tgctgaaagg caacaacatc accaaaccgg ccgacttctc tacctacagc 60 atcacctgga cggccgacac caagccttat gtgaatgccg acagctggac ctccaccggc 120 accttcccga ccgtggccta caactacagc ccggtcagcg cacaatgcgt gaaggacaaa 180 ctgcctggct atgccggcgt gggtaaaaat ctggccacgc tgaccagcac agcctgtaaa 240 taatctcacg cagaggcagg cgcatccgct gactcaccgg gttatcccac cctcgagcgg 300 gctcaatgtg gtttatattt ttgctaccga tccactcgag aacccggcaa gatgcacaag 360 cctgacgtcg cttttcagac aaatttaata atcaatagct ttttaattat taacggttat 420 aagcgacagg tcggaactcc gaccacaaaa agtgacgcct gtcataattc gaaaaaacat 480 aatagatact aataatgaac cggcgttcca actattgaaa tgtatccttt ttgagctaaa 540 actgagcaca ggttgatgcg tgtgattcgg ttttacttat ctatacccga cgtgattcat 600 gcgactaaca gaaaacatgg cgggaatatc cctaccgcat caaccataag taagggcatg 660 tggaacgata aatgcccatc tcatattttt ttaaaaaaca ggaactcgac tcatgaaatc 720 actcattact ccgattgccg ccggcctgct gttggcattc agtcaataca gcctggccgc 780 ggataccggc ggttatacca aaacggacgg tggtgacgtc tccggcgccg tgaagaaaac 840 ggccagctca atgcaggaca tcgtcaatat cattgaagcc gctaaagtcg acgctaacgg 900 caaaaaagtg aaaggcgggg cttatccgct ggtgatcacc tataccggca acgaagacag 960 cctgattaat gccgccgccg ccaatatctg cggccagtgg agcaaagatg cccgtggcgt 1020 ggaaatcaaa gatttcacca aagggctcac cattatcggt gccaacggtt cttccgctaa 1080 cttcggtatc tggatcgtca actcttccga catcgtggta cgcaacatgc gtatcggcta 1140 cctgccgggc ggggctcagg acggcgatat gttccgtatc gacaactcgc cgaacgtctg 1200 gctggatcat aacgaactgt ttgccgctaa ccacgagtgc gacggtacta aagatggcga 1260 caccaccttc gaatccgcga tcgatatcaa aaaaggcgcg acttacgtca ccatctctta 1320 caactacatc cacggcgtga agaaagtcgg cctgtctggt ttcagctcct ccgacacggc 1380 tgaacgcaac atcacctatc accacaacat ctacagcgac gtcaatgctc gtctgccgtt 1440 gcagcgcggt ggtaacgtcc atgcctacaa caacctgtac accggtatca ccagttccgg 1500 cctgaacgtc cgtcagaacg gcaaggcgct gatcgaaaac aactggttcg aaaacgcggt 1560 gagcccggtc acctcccgct atgacggcag caacttcggc acctgggtgc tgaaaggcaa 1620 caacatcacc aaaccggctg atttcgccac ctacaacatc acctggacgc cggatacaaa 1680 agagtaccgc aacgctgaca cctggacctc cactggcacc tacccgaccg tgccttatag 1740 ctacagcccg gttagcgcac agtgcgtgaa agacaaactg gccaactacg ccggtgtagg 1800 caaaaacctg gccacgctgg ccagctcagc ctgtaaataa tccgttcccg tcggcatcgc 1860 tgatgcctgg aacgccctcc ncccggctct ccncccggct ntgggcgtta tcgcaagatt 1920 cattctggac gggagagcac gctttactcg cccgtttttt atttatccgc tcctcgccat 1980 caggcaggac aagccccccc ggcataggga tcaagccatg atgaaacaca ccctgttatt 2040 cacattatta ttcagtacca gcgtcctgac cgctcaggcc gccagt 2086 // |
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