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EBI DbfetchID M14509; SV 1; linear; genomic DNA; STD; PRO; 4978 BP. XX AC M14509; J03385; M19410; XX DT 07-JUN-1987 (Rel. 12, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE E.chrysanthemi pectate lyase E and A (pelE, pelA) genes, complete cds. XX KW pectate lyase. XX OS Erwinia chrysanthemi OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Dickeya. XX RN [1] RP 2946-4978 RX PUBMED; 3536853. RA Keen N.T., Tamaki S.; RT "Structure of two pectate lyase genes from Erwinia chrysanthemi EC16 and RT their high-level expression in Escherichia coli"; RL J. Bacteriol. 168(2):595-606(1986). XX RN [2] RP 1-3212 RX PUBMED; 3042750. RA Tamaki S.J., Gold S., Robeson M., Manulis S., Keen N.T.; RT "Structure and organization of the pel genes from Erwinia chrysanthemi RT EC16"; RL J. Bacteriol. 170(8):3468-3478(1988). XX CC Draft entry and computer-readable sequence of [1] kindly provided CC by N.T.Keen, 29-JAN-1987, and for [2] 31-MAY-1988. XX FH Key Location/Qualifiers FH FT source 1..4978 FT /organism="Erwinia chrysanthemi" FT /mol_type="genomic DNA" FT /db_xref="taxon:556" FT sig_peptide 1099..1194 FT /note="pectate lyase A signal peptide (putative); putative" FT CDS 1099..2280 FT /codon_start=1 FT /transl_table=11 FT /note="pectate lyase A, precursor (EC 4.2.2.2)" FT /db_xref="GOA:P0C1A2" FT /db_xref="InterPro:IPR002022" FT /db_xref="PDB:1JRG" FT /db_xref="UniProtKB/Swiss-Prot:P0C1A2" FT /protein_id="AAA24843.1" FT /translation="MMNKASGRSFTRSSKYLLATLIAGMMASGVSAAELVSDKALESAP FT TVGWASQNGFTTGGAAATSDNIYIVTNISEFTSALSAGAEAKIIQIKGTIDISGGTPYT FT DFADQKARSQINIPANTTVIGLGTDAKFINGSLIIDGTDGTNNVIIRNVYIQTPIDVEP FT HYEKGDGWNAEWDAMNITNGAHHVWIDHVTISDGNFTDDMYTTKDGETYVQHDGALDIK FT RGSDYVTISNSLIDQHDKTMLIGHNDTNSAQDKGKLHVTLFNNVFNRVTERAPRVRYGS FT IHSFNNVFKGDAKDPVYRYQYSFGIGTSGSVLSEGNSFTIANLSASKACKVVKKFNGSI FT FSDNGSVLNGSAVDLSGCGFSAYTSKIPYIYDVQPMTTELAQSITDNAGSGKL" FT mat_peptide 1195..2277 FT /note="pectate lyase A" FT misc_feature 2295..2312 FT /note="pelA rho-independent transcription terminator" FT misc_feature 2325..2723 FT /note="3' end of partially deleted pelD gene" FT misc_feature 2736..2768 FT /note="pelD rho-independent transcription terminator" FT mRNA <3198..4404 FT /note="pelE mRNA" FT CDS 3198..4355 FT /codon_start=1 FT /transl_table=11 FT /note="pectate lyase E, precursor (EC 4.2.2.2)" FT /db_xref="GOA:P04960" FT /db_xref="InterPro:IPR012334" FT /db_xref="PDB:1PCL" FT /db_xref="UniProtKB/Swiss-Prot:P04960" FT /protein_id="AAA24844.1" FT /translation="MKNTRVRSIGTKSLLAAVVTAALMATSAYAAVETDAATTGWATQN FT GGTTGGAKAAKAVEVKNISDFKKALNGTDSSAKIIKVTGPIDISGGKAYTSFDDQKARS FT QISIPSNTTIIGVGSNGKFTNGSLVIKGVKNVILRNLYIETPVDVAPHYESGDGWNAEW FT DAAVIDNSTNVWVDHVTISDGSFTDDKYTTKDGEKYVQHDGALDIKKGSDYVTISYSRF FT ELHDKTILIGHSDSNGSQDSGKLRVTFHNNVFDRVTERAPRVRFGSIHAYNNVYLGDVK FT HSVYPYLYSFGLGTSGSILSESNSFTLSNLKSIDGKNPECSIVKQFNSKVFSDKGSLVN FT GSTTTKLDTCGLTAYKPTLPYKYSAQTMTSSLATSINNNAGYGKL" FT sig_peptide 3198..3287 FT /note="pectate lyase E, signal peptide" FT mat_peptide 3288..4352 FT /note="pectate lyase E" XX SQ Sequence 4978 BP; 1407 A; 1314 C; 1069 G; 1188 T; 0 other; acgcgtggac tggtatagtt ttctgacgca atcagttcaa tgtgctcttc ctgacgcacc 60 acttcttgct gcattgcttg ccatagctcg gcatcgtaat cggcaatgtt catttcacgc 120 tttaacatcc gcattctccg tactcagcta acttgcttct ttattaaaaa tcacccagag 180 gccgtgggta catagtgtaa accgttttgg actggcgatg atagagcttg acagaggttt 240 ttacgcaaac gattagctac aggctacaca aggcttctcg tctcgccggg atttatcgtt 300 ctggctgact ttttctcatt ctccacctgc aattttttca tgcgcgcgat tctgtttcgc 360 cacaccgttt ccctgaacat caccaggtct ttacaggccg ctcacttcta tcagcggatg 420 ttccaccata atccggaact gaaagagatc ttcaatatga gtaatcagcg taatggcaat 480 cagcgtgaag cgctgttcaa taccatttgt gtggaccggt cgcctttatg caatttatcg 540 cgcgccaact attggaattt ggggtgaccg aatcgcagat tcattacgaa tgttttggtc 600 cacataaagt gatatagccg acaaaaatta ctttcattta tgtcaacagg ccggtcatgg 660 cctgttttat ttcccaccaa taaatgccgt cacgtcttat ttccaccgcg ccctgatgaa 720 tgtttcatct cttattattt tatgttgaga aataaataca ttacaggaaa aacaactgcc 780 accaatccct tccccgtctt attcccgaac acaaagatga attaatatat tctatataaa 840 taagaaataa aaaacacaat gttacattta aaacggcgtt tcattattgt ctatcattga 900 aaaacaaata aatagaatgt attcattggc agcccttatg cggataccag cgacaaatct 960 tcattattgt taatgaaata tgattaatat atcatgaaga tccgctggca ttccttaagg 1020 tcaataaata aaaataaccc atagatatat atggaaatta tttttaacac ttaagataag 1080 agtcaactaa ggaaaaatat gatgaacaaa gcttcaggac gttcttttac ccgctcttca 1140 aaatatctgc tggctacctt gatcgccggt atgatggcct ctggtgtttc cgctgccgag 1200 ttggttagcg ataaagcgtt ggaatctgcc cccaccgtcg gctgggcgtc gcagaatggt 1260 ttcacgaccg gcggtgccgc tgcaaccagc gacaatatct acatcgtcac gaatatcagc 1320 gaattcacca gtgcccttag cgccggtgca gaggcaaaga tcattcaaat taaagggacg 1380 atcgatatca gcggcggtac gccttacacc gatttcgcgg atcaaaaagc ccgtagccag 1440 attaacattc cagccaatac tacggttatc gggcttggca ccgacgctaa attcatcaac 1500 ggctctctga ttattgacgg tacggacggc accaataacg tcatcatccg taacgtctat 1560 atccagacgc cgattgacgt agaaccgcac tacgaaaaag gtgatggctg gaacgccgag 1620 tgggacgcca tgaatatcac caatggcgca caccatgtgt ggatcgatca tgtcaccatc 1680 agtgacggta acttcaccga cgacatgtac accaccaaag acggtgaaac ctacgtgcag 1740 catgacggcg ctctggatat caagcgcggt tccgactacg taaccatctc aaacagcctg 1800 atcgaccagc acgacaaaac catgctgatc ggccacaacg acacgaactc cgctcaggac 1860 aaaggcaagc tgcatgtcac gctgttcaac aacgtattca atcgcgtaac cgaacgcgcg 1920 cctcgcgtac gttacggcag catccacagc ttcaacaacg ttttcaaagg tgatgcgaaa 1980 gatccggtat accgttacca atacagcttt ggtatcggta ccagcggcag cgtgttgtcc 2040 gaaggcaaca gcttcacgat tgcaaacctg agcgccagca aagcatgcaa agttgtgaag 2100 aaattcaacg gctccatctt ctctgacaac ggttccgtcc tgaacggcag cgccgtcgat 2160 ctgtctggtt gcggtttcag cgcctacacc agcaagatcc cttacattta cgatgttcag 2220 ccgatgacga ccgaactggc gcagtccatt acggacaacg ccggctctgg caaactgtaa 2280 tttcgcgtat ccaacggctg cccaggcagc cgtttttatt ccccgcttta aaccggttcg 2340 accgtcgtac tccgcgctta cgcttcggta acgttcatgc ctacaacaac gtctacaccg 2400 gcgatgtcaa tcataaagct taccgctatc agtacagctt cggcaccagc ggcagcctgc 2460 tgtctgagaa caatgcattt accattgata acctcaagaa gatcaacggc cgcgataagg 2520 aatgtagcgt cgtcaaagcg tttaacggca agatcttctc cgataaagga tcaatcatca 2580 acggcgcctc gtacaacctg aacggttgcg gctttggctt caacacctat tccgccaaga 2640 tcccatacaa atattcagct caaaccatca ccaccaacct ggcaaacagc atcagcagca 2700 atgcggggta cggcaaactg taatttgtat tcacttggga aggccgccgg aaacggcggc 2760 cttccctcct caccaagtca tcatgatctc aaattttgta acaaccgtat cagcggggaa 2820 aaagcagtgt ctatcacggc aaataggggc ttcccgcatt aataaggcac ttaaattaat 2880 aaaattatat ttaataaaaa tcgaattatt gtttaacggt tttatttata tcaggcaaaa 2940 attccggatc atatgactga atttaaaaga aaattcaatt caacatccat tagcaaaagt 3000 taccggtctc gatcacagtt tagataaaat taacaacagc cataaaaaaa cgagattttg 3060 atcgcaaaac atctcccccg aaatgcttaa aaatccacct tgccgaggga caaaatggca 3120 tttcattttt tcacaaacgg ctttttgaat tcttaacaat gcatttggat tagcgcctac 3180 agcgaaggaa acggtctatg aaaaacacgc gtgtacgttc tatcggaact aaaagtttac 3240 tggctgccgt tgtaacagca gcactgatgg ctacctctgc ttatgcggca gtagaaactg 3300 atgccgcaac aactggctgg gcaacgcaaa acggcggcac taccggtggc gcaaaggcag 3360 caaaagcagt tgaagtaaaa aacattagcg actttaaaaa agccctgaat ggaaccgact 3420 catcagccaa aatcatcaaa gtaacagggc cgattgatat cagtggcggc aaagcctaca 3480 ccagtttcga tgatcagaaa gcgcgtagcc agatcagcat tccgtccaat accaccatta 3540 tcggtgttgg cagcaatggt aaattcacca acggttctct ggtgatcaaa ggcgtaaaaa 3600 acgttatcct gcgtaacctg tatattgaaa cgccggtaga cgtagcaccg cattatgaaa 3660 gtggggatgg ctggaacgcc gagtgggatg cagccgttat cgacaactct accaacgtct 3720 gggttgacca cgtcaccatc agcgatggta gcttcaccga cgacaaatac accaccaaag 3780 atggtgaaaa atacgttcag cacgacggcg cactggatat caagaaaggg tctgactacg 3840 tcaccatttc ttacagccgc ttcgaactgc acgacaaaac tatcctgatc ggccacagcg 3900 acagcaacgg ctctcaggat tccggcaaac tgcgcgtcac cttccacaac aacgtgttcg 3960 accgcgtgac tgaacgtgcc ccgcgcgtac gtttcggtag catccacgct tacaacaacg 4020 tttatctggg cgacgtgaaa cacagcgtct atccgtacct gtacagcttc ggcctgggca 4080 ccagcggcag catcctgtct gagtccaact ccttcacgct ctccaacctg aagagcattg 4140 atggtaaaaa cccagaatgt agcatcgtga agcaattcaa cagcaaggtg ttctccgata 4200 aaggctcact ggttaacggc tcaaccacca ccaagctgga tacctgtggt ctgacggcgt 4260 acaaaccgac tctgccgtac aaatattcgg ctcagaccat gaccagcagc ctggctacca 4320 gcatcaacaa caacgcaggt tacggcaaac tgtaataccc ctgtcgacaa cagcggccgc 4380 ttgaaaaagc ggccgttttt tgcatcaatc gcaggaagaa aaggtcatca ccatgaaaat 4440 aaccacctgg aaacgcacgc tttttctcgg ttccctactg tgtctgccga tatccttcgc 4500 gcaggccgca aacatcgcgc cagaatcccc cgcgtcccct tcacagccga tattaaatgt 4560 cacgacgctg ggaccaaaca ccctgattag cggccgggta gcgtatcgtg atatacgttt 4620 tcccgccacg ttgttcatca aggatcaacg cggcgtagag cacagtgtaa aaaccgatat 4680 ccagggacga ttttatgcgg atgtctcttc aatggtcgca ccgctgcgcc tgtcagccgt 4740 tgaagccggc gggcaaaact gccttgccag caatcagctt cgtgcggtgt gcctgagtgc 4800 actggttccc caattacgtg atggtcatga gaatcgtatt aacatcaacc cgttgaccga 4860 tcgtatttta tccgatgtcg ctgcatcggc cggttatatt ggcccacagc aactgatcga 4920 cgccacgacg ctgccatctt tatcgacagc agcctgggaa accgcttacc gtgaattc 4978 // |
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