EBI Dbfetch

ID   M14340; SV 1; linear; genomic DNA; STD; PRO; 1689 BP.
XX
AC   M14340;
XX
DT   16-JUL-1988 (Rel. 16, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   Streptococcus pneumoniae DpnI endonuclease (dpnC) and dpnD genes, complete
DE   cds; and unknown genes.
XX
KW   .
XX
OS   Streptococcus pneumoniae
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus.
XX
RN   [1]
RP   1-1689
RX   DOI; 10.1016/0092-8674(86)90698-7
RX   PUBMED; 3019562.
RA   Lacks S.A., Mannarelli B.M., Springhorn S.S., Greenberg B.;
RT   "Genetic basis of the complementary DpnI and DpnII restriction systems of
RT   S. pneumoniae: an intercellular cassette mechanism";
RL   Cell 46(7):993-1000(1986).
XX
RN   [2]
RP   1-1689
RX   PUBMED; 2844782.
RA   de la Campa A.G., Springhorn S.S., Kale P., Lacks S.A.;
RT   "Proteins encoded by the DpnI restriction gene cassette. Hyperproduction
RT   and characterization of the DpnI endonuclease";
RL   J. Biol. Chem. 263(29):14696-14702(1988).
XX
RN   [3]
RP   1-1689
RA   Lacks S.;
RT   ;
RL   Submitted (26-APR-1993) to the EMBL/GenBank/DDBJ databases.
RL   Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000,
RL   USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1689
FT                   /organism="Streptococcus pneumoniae"
FT                   /strain="S. pneumoniae 193"
FT                   /mol_type="genomic DNA"
FT                   /clone="S. pneumoniae 777[pLS207]"
FT                   /db_xref="taxon:1313"
FT   CDS             <1..96
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="unknown"
FT                   /note="orfL"
FT                   /db_xref="UniProtKB/TrEMBL:Q54825"
FT                   /protein_id="AAA88576.1"
FT                   /translation="DITNQEGRNVCKATFTMFVTGQRSEERRVSI"
FT   promoter        186..214
FT   CDS             277..1041
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="dpnC"
FT                   /product="DpnI endonuclease"
FT                   /note="acts on 5' GmeATC"
FT                   /db_xref="GOA:P0A459"
FT                   /db_xref="InterPro:IPR010324"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A459"
FT                   /protein_id="AAA88577.2"
FT                   /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNHF
FT                   ENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYTKN
FT                   FEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDGQVR
FT                   DPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKNIFVK
FT                   NNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
FT   CDS             1038..1499
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="dpnD"
FT                   /product="unknown"
FT                   /db_xref="GOA:P0A3T4"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0A3T4"
FT                   /protein_id="AAA88578.1"
FT                   /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDCV
FT                   RWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKYTV
FT                   EITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
FT   CDS             complement(1550..>1689)
FT                   /codon_start=3
FT                   /transl_table=11
FT                   /product="unknown"
FT                   /note="orfR"
FT                   /db_xref="UniProtKB/TrEMBL:Q9LCT8"
FT                   /protein_id="AAF34272.1"
FT                   /translation="AGVGLNNSNLFVSMPTAFQMFFSNGIVVSSLLAIVLNAVLNHKKK
FT                   "
XX
SQ   Sequence 1689 BP; 609 A; 224 C; 351 G; 505 T; 0 other;
     gatatcacca atcaagaggg cagaaatgtc tgtaaggcaa cctttaccat gtttgtcaca        60
     ggccaaaggt ctgaggaaag acgggtaagt atataaagaa aggtgagtgg gataaattta       120
     tctcactcat ttttaattta tgagatatgt tagaaggaaa tataaaagag agtgatgaaa       180
     tacaaatgta ataggtacat atatgtggta tactagttat agtagatttt aattgcttat       240
     atcgagtgag ttctcatatt agtgaggaat ttttaaatgg aattacactt taatttagaa       300
     ttagtagaaa cctataaaag taatagccaa aaggcacgta tcttaacgga ggattgggtt       360
     tatcgtcagt cttattgccc taattgtggg aacaatccct taaatcattt tgaaaataat       420
     cggcctgtag cagattttta ctgtaatcat tgtagtgagg agtttgaact aaagagcaaa       480
     aaaggaaatt tttcatcaac aatcaatgat ggtgcttatg caacgatgat gaagcgtgtg       540
     caggcagata ataatcctaa tttctttttt ttaacttaca caaaaaattt tgaggtaaat       600
     aactttcttg tccttccgaa gcaatttgtt acaccgaaat cgattattca aagaaaacca       660
     cttgcaccaa ctgctagacg agcaggttgg attggttgta acattgattt atcacaagta       720
     ccttctaaag gaaggatatt tcttgtgcaa gatggacaag ttagagatcc agaaaaagtt       780
     acaaaagaat ttaagcaagg tttattttta aggaagagct ctctgtcatc aagaggttgg       840
     acaatagaaa ttctaaattg tatagataag atagagggtt cagaatttac ccttgaagat       900
     atgtatcgtt ttgaaagtga cctaaaaaat atctttgtta agaacaatca tatcaaagaa       960
     aagattaggc aacagcttca aatattaaga gacaaagaaa taatagaatt taaaggtaga      1020
     ggaaagtatc ggaaattatg aaaacgaaac aacttgttgc atcagaagag gtgtatgatt      1080
     tcttaaaagt catctggcct gattatgaaa ctgaaagccg ttacgataac ctaagtttaa      1140
     tcgtctgtac cttatcagat cccgattgtg tgagatggtt atctgaaaat atgaaatttg      1200
     gtgacgaaaa acaactagct ttgatgaagg aaaaatatgg gtgggaagta ggagataaat      1260
     tgccagagtg gctacatagc tcctatcata gattattgtt aataggtgaa ttattggaaa      1320
     gcaatctaaa actgaaaaag tatacagtag aaattacaga aactttatca cgtttagtaa      1380
     gtatagaggc tgaaaatcca gatgaagccg aacgacttgt aagagaaaag tataagagtt      1440
     gtgaaattgt tcttgatgca gatgattttc aggactatga cactagcata tatgaatagg      1500
     tagatgtttt tattttgtca cacaaaaaag aggctcgcac ctctttttct tatttctttt      1560
     tatgatttaa tacggcattg aggacaatag cgagtaggct ggatacgacg attccgtttg      1620
     agaagaacat ttggaaggct gtcggcatgc tgacaaagag attactgttg ttgagaccga      1680
     cacctgcag                                                              1689
//