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EBI DbfetchID M11035; SV 1; linear; genomic DNA; STD; PRO; 3677 BP. XX AC M11035; M35690; XX DT 18-NOV-1986 (Rel. 10, Created) DT 02-SEP-1999 (Rel. 60, Last updated, Version 3) XX DE P.syringae tryptophan 2-monooxygenase (iaaM) and indoleacetamide hydrolase DE (iaaH) genes, complete cds. XX KW indoleacetamide hydrolase; indoleacetic acid; tryptophan 2-monooxygenase; KW tryptophan-2-oxyreductase. XX OS Pseudomonas syringae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 202-3677 RX DOI; 10.1073/pnas.82.19.6522 RX PUBMED; 16593610. RA Yamada T., Palm C.J., Brooks B., Kosuge T.; RT "Nucleotide sequences of the Pseudomonas savastanoi indoleacetic acid genes RT show homology with Agrobacterium tumefaciens T-DNA"; RL Proc. Natl. Acad. Sci. U.S.A. 82(19):6522-6526(1985). XX RN [2] RP 1-312 RX PUBMED; 2120185. RA Gaffney T.D., da Costa e Silva O., Yamada T., Kosuge T.; RT "Indoleacetic acid operon of Pseudomonas syringae subsp. savastanoi: RT transcription analysis and promoter identification"; RL J. Bacteriol. 172(10):5593-5601(1990). XX CC The iaaH and iaaM genes are part of an operon that is borne on a CC plasmid, pIAA, in oleander strains of the pathogen. There appears CC to be no genetic transformation of host tissue by P.savastanoi. No CC Pribnow boxes were found, though potential ribosome binding sites CC are located at positions 435-439 and 2164-2168. Expression of iaaH CC depends upon the promoter for iaaM. CC Draft entry and computer-readable sequence for [1] kindly submitted CC by T.Yamada, 14-JAN-1986. CC Draft entry and computer-readable sequence for [J. Bacteriol. CC (1990) In press] kindly submitted CC by T.D.Gaffney, 28-JUN-1990. XX FH Key Location/Qualifiers FH FT source 1..3677 FT /organism="Pseudomonas syringae" FT /sub_species="savastanoi" FT /mol_type="genomic DNA" FT /db_xref="taxon:317" FT misc_feature 19..47 FT /note="indoleacetic acid operon promoter" FT mRNA 54..>3537 FT /note="indoleacetic acid mRNA" FT CDS 82..312 FT /codon_start=1 FT /transl_table=11 FT /note="promoter proximal ORF" FT /db_xref="UniProtKB/TrEMBL:Q52503" FT /protein_id="AAA25851.1" FT /translation="MPGRTPANWLDKAMVAVARIRQRKPQAAVPAALLQAPHAMEFVWS FT TTKTFTECHCPAYTPYTQASAWSASAVVSAY" FT CDS 454..2127 FT /codon_start=1 FT /transl_table=11 FT /note="tryptophan 2-monooxygenase (EC 1.13.12.3)" FT /db_xref="GOA:P06617" FT /db_xref="InterPro:IPR000759" FT /db_xref="InterPro:IPR002937" FT /db_xref="UniProtKB/Swiss-Prot:P06617" FT /protein_id="AAA25852.1" FT /translation="MYDHFNSPSIDILYDYGPFLKKCEMTGGIGSYSAGTPTPRVAIVG FT AGISGLVAATELLRAGVKDVVLYESRDRIGGRVWSQVFDQTRPRYIAEMGAMRFPPSAT FT GLFHYLKKFGISTSTTFPDPGVVDTELHYRGKRYHWPAGKKPPELFRRVYEGWQSLLSE FT GYLLEGGSLVAPLDITAMLKSGRLEEAAIAWQGWLNVFRDCSFYNAIVCIFTGRHPPGG FT DRWARPEDFELFGSLGIGSGGFLPVFQAGFTEILRMVINGYQSDQRLIPDGISSLAARL FT ADQSFDGKALRDRVCFSRVGRISREAEKIIIQTEAGEQRVFDRVIVTSSNRAMQMIHCL FT TDSESFLSRDVARAVRETHLTGSSKLFILTRTKFWIKNKLPTTIQSDGLVRGVYCLDYQ FT PDEPEGHGVVLLSYTWEDDAQKMLAMPDKKTRCQVLVDDLAAIHPTFASYLLPVDGDYE FT RYVLHHDWLTDPHSAGAFKLNYPGEDVYSQRLFFQPMTANSPNKDTGLYLAGCSCSFAG FT GWIEGAVQTALNSACAVLRSTGGQLSKGNPLDCINASYRY" FT CDS 2170..3537 FT /codon_start=1 FT /transl_table=11 FT /note="indoleacetamide hydrolase" FT /db_xref="GOA:P06618" FT /db_xref="InterPro:IPR000120" FT /db_xref="InterPro:IPR020556" FT /db_xref="UniProtKB/Swiss-Prot:P06618" FT /protein_id="AAA25853.1" FT /translation="MHEIITLESLCQALADGEIAAAELRERALDTEARLARLNCFIREG FT DAVSQFGEADHAMKGTPLWGMPVSFKDNICVRGLPLTAGTRGMSGFVSDQDAAIVSQLR FT ALGAVVAGKNNMHELSFGVTSINPHWGTVGNPVAPGYCAGGSSGGSAAAVASGIVPLSV FT GTDTGGSIRIPAAFCGITGFRPTTGRWSTAGIIPVSHTKDCVGLLTRTAGDAGFLYGLL FT SGKQQSFPLSRTAPCRIGLPVSMWSDLDGEVERACVNALSLLRKTGFEFIEIDDADIVE FT LNQTLTFTVPLYEFFADLAQSLLSLGWKHGIHHIFAQVDDANVKGIINHHLGEGAIKPA FT HYLSSLQNGELLKRKMDELFARHNIELLGYPTVPCRVPHLDHADRPEFFSQAIRNTDLA FT SNAMLPSITIPVGPEGRLPVGLSFDALRGRDALLLSRVSAIEQVLGFVRKVLPHTT" XX SQ Sequence 3677 BP; 813 A; 917 C; 1046 G; 901 T; 0 other; ccacaaaccg gcaagacgtt gaaaatcaat ttcaagcatt gtatgttttt ggtgggacgc 60 cacttggaaa atctggtgct gatgcccgga cggactcctg cgaactggct ggacaaggcg 120 atggtggcag tggcccgaat cagacagcgc aagccccagg cggcagtacc cgcggcgctc 180 ctccaagccc cgcacgctat ggaattcgtt tggagcacca ccaaaacctt taccgaatgc 240 cattgtccgg cttacacccc ctatacgcaa gccagtgcgt ggtcggccag cgcggtcgtt 300 agtgcttatt aattttagag gactagcatg gtttgaagga atggttcagg tgttttggat 360 tttggcggct gccgttaaaa ggtgctgttt cagtttcatt taaagaatcg taatccgggt 420 agcacgtaag gtcgaagagg gtagagtcgc gttatgtatg accattttaa ttcacccagt 480 attgatattt tgtacgacta cggtcccttt ctgaaaaaat gtgaaatgac gggaggcata 540 ggcagctatt cagccggaac gcccacccct cgggtagcga tagtcggtgc cggcatcagt 600 gggctggtcg ctgcaactga actattacgt gcgggagtca aggacgttgt cttatatgaa 660 tcgcgtgatc gaatcggggg acgggtatgg tctcaagttt tcgatcagac tcgtccacgt 720 tacattgcag aaatgggtgc gatgcgcttt cctcccagtg caactggcct tttccactac 780 ctgaaaaagt ttggtatttc gacgtcgacc acctttccgg atcctggtgt ggtggacacg 840 gagctgcatt accgtggcaa gcgctatcac tggccagcgg gcaaaaagcc gcccgaatta 900 ttcaggcgag tctatgaggg gtggcagtct ctattgtccg aaggttacct ccttgaaggc 960 ggttctttag ttgccccgct ggacattacc gcaatgctga agtcgggtcg tctggaagag 1020 gcagcgatcg catggcaggg atggctcaat gtattccggg attgttcatt ctataacgcg 1080 attgtctgta tttttactgg ccgccatccg ccaggcggcg acagatgggc tcgtcctgaa 1140 gactttgagc tgtttggctc gcttggcata ggctcgggcg ggtttttgcc agtctttcag 1200 gctggcttta cggaaatact gcggatggtt atcaacggat accaaagtga ccagcgactg 1260 attccggacg ggatatccag tctggccgcg agactcgctg atcagtcgtt tgacggcaaa 1320 gcgttaaggg accgcgtttg ttttagccgg gtaggtcgca tttccagaga agctgaaaaa 1380 atcatcatcc agacggaagc aggagaacag cgtgtatttg atcgagtaat tgtcactagc 1440 agtaatcggg ccatgcaaat gattcactgc ctcacggata gcgagagctt tctgagtcgt 1500 gatgtcgctc gtgctgtccg cgaaacccat ctgacaggtt catcgaagct tttcattctc 1560 acccgaacca aattctggat aaaaaacaag cttcccacca ccatccagtc ggacggtctg 1620 gtgcgcggcg tctattgtct ggattatcag cccgatgaac ctgaggggca tggcgttgtt 1680 ctgctcagtt acacgtggga agacgacgct caaaaaatgc tggcgatgcc tgacaagaaa 1740 acgcgttgcc aggtactggt tgatgacctt gctgcgatac acccgacgtt cgccagttat 1800 ctcctgcccg ttgatgggga ttatgagcgg tatgtattgc accatgactg gctcaccgat 1860 ccccattctg cgggcgcttt caaactcaat tatcccggcg aggacgttta ctcgcagcga 1920 ttgttttttc aaccaatgac agcgaacagt cccaataaag acacggggct ctatctggct 1980 ggctgcagtt gctcttttgc cggagggtgg atcgaaggtg ctgtccagac agcattgaac 2040 agtgcttgcg cggtgctgcg cagcaccgga gggcaactgt caaaaggcaa cccgctggac 2100 tgtatcaacg cctcctatcg ctattaacag tcctgagagc agcgctaagc taatacgggt 2160 gaaaagagca tgcatgaaat aatcacactt gaatcgctgt gccaggcatt ggctgatggc 2220 gagattgccg ctgcggagct gcgcgagcgt gcgctcgata ctgaggcccg cttggcccgt 2280 ttgaactgtt tcatacgtga gggtgatgcg gtcagccagt ttggcgaggc tgatcatgca 2340 atgaagggta ctccactctg gggaatgccg gtttccttca aggataatat ctgtgttcgc 2400 ggtttgccgt tgacagccgg gacgcgagga atgtccggtt tcgtatctga tcaggatgct 2460 gcgattgtca gtcaactcag agccctcggg gctgtggtcg ctggcaagaa caacatgcac 2520 gaactcagtt tcggagtgac ctccatcaat cctcattggg gaaccgtggg aaaccccgtg 2580 gcccccggtt attgtgccgg aggtagtagt ggtggaagtg ccgccgcagt ggcaagtgga 2640 attgttccgc tgtcggtggg gaccgacacg gggggctcga taagaatacc ggcggccttc 2700 tgcggcatta cgggcttcag acccactact ggacgctggt caacggcagg cattatccct 2760 gtttctcata caaaggattg cgttgggttg ctgacacgaa cggcaggcga cgccggattt 2820 ttgtacggac tgttgtcagg caagcagcag tcttttcccc tgagcaggac ggccccctgt 2880 cgaatcggcc taccggtctc catgtggtcc gatctggatg gcgaggtgga gagggcatgc 2940 gtgaatgcac tcagcctgct gcgcaagaca gggttcgagt ttattgaaat tgatgatgcc 3000 gatattgtcg aactgaacca gacactcacg ttcaccgttc cgctttacga attctttgcc 3060 gaccttgctc agtcattgct ctccttgggc tggaagcacg gtatccatca tatttttgca 3120 caggttgatg atgccaacgt gaaaggcatc atcaatcacc accttgggga gggcgctata 3180 aaaccggctc actatttgag ttcactgcaa aatggcgagc tgttgaaaag aaaaatggat 3240 gagctatttg ctcgtcataa tatcgagctc ctggggtacc ccacggtacc ttgccgggta 3300 cctcatctgg atcatgctga ccgaccagag tttttcagtc aggcaattcg caataccgac 3360 ctggccagca atgcgatgct cccctccatt actattcctg tcgggccaga agggcgattg 3420 ccggtcggct tgagctttga tgctctaaga gggcgagacg cacttttgct gagcagagtc 3480 agtgccatag agcaggtatt aggttttgta cgaaaagtat tgccgcatac aacgtaggct 3540 acaggccagc gtgaccatgg ctgcgtagct cttggccagc ttgtcgtagc gggtgccgat 3600 tcggcggttc tcttttagcc agccaaacat ccgctcaatg atgttgcgct gccgatactt 3660 tggacgatcg aagagtc 3677 // |
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