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EBI DbfetchID L49336; SV 1; linear; genomic DNA; STD; PRO; 7518 BP. XX AC L49336; XX DT 04-DEC-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Clostridium longisporum methyl-accepting chemotaxis protein (macA) gene, DE partial cds; and tryptophanyl-tRNA synthetase (trsA), regulatory protein DE (abgG), PTS-dependent enzyme II (abgF), phospho-beta-glucosidase (abgA), DE ORF; putative, and PII-like protein genes, complete cds. XX KW abgA gene; abgF gene; abgG gene; antiterminator; glnB gene; macA gene; KW methyl-accepting chemotaxis protein; phospho-beta-glucosidase; KW PII-like protein; PTS enzyme II; trsA gene; tryptophanyl-tRNA synthetase. XX OS Clostridium longisporum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-7518 RX DOI; 10.1007/s004380050641 RX PUBMED; 9491080. RA Brown G.D., Thomson J.A.; RT "Isolation and characterisation of an aryl-beta-D-glucoside uptake and RT utilisation system (abg) from the gram-positive ruminal Clostridium species RT C. longisporum"; RL Mol. Gen. Genet. 257(2):213-218(1998). XX RN [2] RP 1-7518 RA Brown G.D., Thomson J.A.; RT "Characterisation of the abg system from Clostridium longisporum"; RL Unpublished. XX DR RFAM; RF00230; T-box. XX FH Key Location/Qualifiers FH FT source 1..7518 FT /organism="Clostridium longisporum" FT /strain="B6405" FT /mol_type="genomic DNA" FT /clone="pBGL1" FT /db_xref="taxon:1523" FT CDS <1..328 FT /codon_start=2 FT /transl_table=11 FT /gene="macA" FT /product="methyl-accepting chemotaxis protein" FT /note="This truncated protein contains two potential FT methylation sites in the 3' region.; putative" FT /db_xref="GOA:Q46126" FT /db_xref="InterPro:IPR004089" FT /db_xref="UniProtKB/TrEMBL:Q46126" FT /protein_id="AAC05710.1" FT /translation="LDFMSEDVKKQFNEFILSGEYYYENAEKISSISEDIAAMSEELSA FT SMEEINSMISSMAENTEKSTDNSSKILVKVRETSNAMEEVAVTAEKQNVLAKELNKLIK FT DFKI" FT -35_signal 348..358 FT /note="putative" FT -10_signal 373..380 FT attenuator 413..660 FT /phenotype="transcription attenuator" FT /note="putative" FT RBS 684..692 FT /note="putative" FT CDS 699..1724 FT /codon_start=1 FT /transl_table=11 FT /gene="trsA" FT /product="tryptophanyl-tRNA synthetase" FT /note="T-box gene; putative" FT /db_xref="GOA:Q46127" FT /db_xref="HSSP:1I6M" FT /db_xref="InterPro:IPR002306" FT /db_xref="UniProtKB/Swiss-Prot:Q46127" FT /protein_id="AAC05711.1" FT /translation="MAKEIILTGDRPTGKLHIGHYVGSLKNRVQLQNSGDYRSFIMIAD FT QQALTDNARNPEKIRNSLIEVALDYLAVGIDPLKSTILVQSQIPELNELTMHYLNLVTL FT SRLERNPTVKAEIKQKNFENSIPAGFLIYPVSQAADITAFKATTVPVGEDQLPMIEQAR FT EIVRSFNTIYGKEVLVEPKAVIPKGTIGRLPGTDGKAKMSKSIGNAIYLADEADVIKQK FT VMSMYTDPNHIKVTDPGQVEGNTVFTYLDTFCKDTETLEEMKAHYSRGGLGDVKVKKFL FT NEILQAELEPIRNRRKEFQKDIPEVYRILKEGSEKAREVAAGTLKEVRETIGIEYFNNI FT F" FT terminator 1738..1779 FT /note="putative" FT attenuator 1976..2033 FT /phenotype="terminator/antiterminator stem loops" FT /note="putative" FT RBS 2058..2065 FT /note="putative" FT CDS 2072..2908 FT /codon_start=1 FT /transl_table=11 FT /gene="abgG" FT /product="regulatory protein" FT /function="antitermination" FT /note="regulatory protein similar to bglG; unusual AUU FT start codon which is unverified but was derived from FT homology, the general length of these proteins and the FT position of the RBS; putative" FT /db_xref="GOA:Q46128" FT /db_xref="HSSP:1H99" FT /db_xref="InterPro:IPR011608" FT /db_xref="UniProtKB/TrEMBL:Q46128" FT /citation=[2] FT /protein_id="AAC05712.2" FT /translation="MYTIKKIFNNNSVLALDSEKREIVILGCGIAFKKKVNDKVSEDNV FT EKTFILKQKDASEKFKLLLEDISAEYISLCYDIIEYAKNILDKELNDYIYVTLTDHINY FT VIEACKQGISKPNILIWEIKKFYPKEFAVGLKAIEFIEDELGYKLHEDEAGNIALHLIN FT AQVNEKSDTVEDVYNITKKINDILNIVKYTYNLELDEKTLNYERFITHLRFFFKRLERK FT EITESEDNFLLEQVKNKYTDAYECMLKIEKYLGQELSNEEQLYLMLHIQRVTTREK" FT attenuator 2907..2999 FT /phenotype="terminator/antiterminator" FT /note="putative" FT RBS 3023..3030 FT CDS 3038..4888 FT /codon_start=1 FT /transl_table=11 FT /gene="abgF" FT /product="PTS-dependent enzyme II" FT /function="transmembrane transport of aryl-beta-glucosides" FT /db_xref="GOA:Q46129" FT /db_xref="HSSP:1GPR" FT /db_xref="InterPro:IPR018113" FT /db_xref="UniProtKB/TrEMBL:Q46129" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC05713.1" FT /translation="MKYEQLAKDILKNVGGKENVNSLTHCITRLRFKLKDEGKANTDIL FT KNMDGVVTVVKSGGQYQVVIGNHVPDVFAAVNAVGGLQGASEEDETEEKMNPLNKFIDI FT VSGVFQPILGVLCATGMIKGLNAVLVAAGVLASTDSTYIILNAIGDCLFNFFPIFLGFT FT AAKKFKLNQFTGMAVGAAMVYPAITSFAGQTVDFFGIPVVMPSSSYQSTVIPIILAIYI FT ASKVEKLFKKIIPDMVKTFLVPFATLLVVVPVTFMAIGPISTIAANALGDLTLAIYNFN FT PTIAGLFIGGFWQVFVMFGLHWGLVPIAMNNLAVIGYDPVLATSVAVCFAQTGVVMAIL FT AKTKDKKLKSLCIPAIISGFFGVTEPAIYGITLPRKKPFILSCIAAGVTGGIIGFFESK FT GYSMGGLGIFALPSYINPEGIDRGFYGMVIAMVAGIVVGFILMFVTKLNDEEEVKTTES FT KKEESLVKQEEIVSPIQGEVVTLAEVKDEAFSSGALGKGVAINPIEGKVYAPADGTLTT FT LFPSLHALGITTENGAEILIHVGMDTVQLEGKHFTAKVKQGDKIKKGQLLIEFDKEAIE FT KAGYSTITPVLITNSDQYLDVIETDKRKVDVNSELLTVVI" FT RBS 4902..4908 FT CDS 4916..6337 FT /codon_start=1 FT /transl_table=11 FT /gene="abgA" FT /product="phospho-beta-glucosidase" FT /db_xref="GOA:Q46130" FT /db_xref="HSSP:1PBG" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:Q46130" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC05714.1" FT /translation="MSFDLSFPKSFLWGGATAANQFEGAYNEDGKGLSIQDIAPKGVMG FT PITEVPTEDNMKLIGIDFYHRYKEDIKLFAEMGFKTFRLSIAWSRIFPNGDDEIPNEKG FT LEFYDKVFDELQRYGIEPLVTLSHYETPLNLSKKYNGWANRDLIGFYERYVRTVFTRYK FT DKVKYWLTFNEINSAIHAPYMSAGIWTDKSELSKQDLYQAMHHELVASALAVKIGHEIN FT PDFKIGCMILGIPVYPLTPHPDDLIEKMRVERESLFFADVHARGKYPRYMNRLFKENNI FT EIKWHEDDAEILSNVVDFISFSYYMSSCATADEEKKKAGAGNLLAGVPNPYLKASEWGW FT QIDPKGLRLILNELYDRYEKPLFIVENGLGAVDELVTDENGNKTVNDDYRIKYLNDHLV FT QVAEAIEDGVELMGYTTWGCIDLVSASTAELKKRYGFIYVDRHDDGSGTLERYKKKSFN FT WYKEVIATNGKSLER" FT terminator 6351..6391 FT RBS 6435..6441 FT CDS 6446..6742 FT /codon_start=1 FT /transl_table=11 FT /note="ORF; putative" FT /db_xref="UniProtKB/TrEMBL:Q46131" FT /protein_id="AAC05715.1" FT /translation="MDIEKVIKFIDKSIDEKENYNELIIKELIKDKNGLIPLLVKIDIF FT YFLYILKVLDEVILILYSKDFISEFKRKIYIRYDKLSNINVNNHETIKDKCNN" FT RBS 6843..6850 FT CDS 6858..7196 FT /codon_start=1 FT /transl_table=11 FT /product="PII-like protein" FT /note="putative" FT /db_xref="GOA:Q46132" FT /db_xref="HSSP:1UL3" FT /db_xref="InterPro:IPR017918" FT /db_xref="UniProtKB/TrEMBL:Q46132" FT /protein_id="AAC05716.1" FT /translation="MKKIEAVIRPEKLEELKDALKKGNINGITVDQVMGCGQQHGWTEH FT YRGSEIMVNFLPKVEVKVIVDDSKVQEVINLIIDTVKTGEVGDGKIFVSDVSECIRIRT FT GEKGLDAL" FT terminator 7211..7252 FT /note="putative" XX SQ Sequence 7518 BP; 2997 A; 832 C; 1377 G; 2312 T; 0 other; tctagatttt atgagcgagg atgttaaaaa gcaatttaat gaatttattt tatctggtga 60 atattattat gaaaatgcag aaaaaataag ttctatatca gaagatattg ctgctatgtc 120 tgaagaactt agtgcatcaa tggaggaaat aaattcaatg atttcaagta tggctgaaaa 180 tactgaaaag tcaacagata actcttctaa gattttagtt aaagttagag aaacttctaa 240 tgctatggaa gaagtcgcag taacagcaga gaaacagaat gtgttggcaa aggaattaaa 300 taaattaata aaagatttca agatataata aatacttttg agttaaatat tgacaactag 360 gggtgaaaaa cttaatatga taatataagt taataaagaa taatcttgtg ataaggaaga 420 gtaattaaat ttattttcta cacagagagt taggagtatg ctggaaacct gacagaaaag 480 atttaatgaa tgggcctttg aagaataaag agaaattatt aaagagtatc ggatatgggg 540 ctcgtgcacc ttatagcata gttagtatgt tagtactaaa acatagagag gtatttaaat 600 acaatttagg tggtaccgcg gaataaaact ccgtcctatt aaaatatagg atggagtttt 660 ttgatgtcta tgagaataaa aaagaggagt ggaaaaatat ggcaaaagaa attatattaa 720 caggtgatag accaacagga aaattacata ttgggcatta tgtaggttca ttaaaaaata 780 gagttcagct tcaaaattca ggagattata gaagctttat aatgatagct gatcagcagg 840 ctttaactga taatgcaaga aacccagaaa agattagaaa tagtttgata gaggtagcct 900 tagactattt ggcagtaggt attgatccat taaaatctac tattttagta caatcacaaa 960 taccagaatt aaatgaactt actatgcatt atttaaattt agttactctt tcaagattag 1020 agcgtaaccc tacagtaaag gcagaaataa agcaaaagaa ttttgaaaat agtattccag 1080 caggattttt aatatatcca gtaagtcagg ctgctgatat cactgcattt aaggctacta 1140 cagtgccagt tggggaagat cagcttccaa tgatagagca agcaagagaa atagtaagaa 1200 gttttaacac tatatacgga aaagaagttt tagtagaacc aaaggctgtt attccaaaag 1260 gaactattgg aagacttcct ggaacagatg gtaaggctaa aatgagtaaa tctataggta 1320 atgctatcta tttagctgat gaagcagatg taataaaaca aaaggtaatg tctatgtata 1380 cagaccctaa tcatataaag gtaacagatc cagggcaagt tgaaggaaat actgtattta 1440 cctatttaga taccttctgc aaagatacag aaacattaga agaaatgaaa gctcattata 1500 gtagaggtgg tcttggagat gttaaggtta aaaaattctt aaatgaaata ttacaagcag 1560 aacttgaacc tattagaaat agaagaaagg aatttcaaaa agatattcct gaagtttata 1620 gaattttaaa agaaggcagt gaaaaggcaa gagaagtggc agcagggact ttaaaagaag 1680 taagagagac tattggtata gaatatttta ataatatatt ctaaaaggaa gtttaataaa 1740 aattagtctt catattaaag gatatgaaga ctaatttttt atgattattg atagagagtc 1800 gaaaaaaatc aatatacgta aatgtaaatt attaaattat taattttaat tgtaattttt 1860 gatggttata taaaaaacat aaaaaaagtt gacattgtga ataaaatata atactatata 1920 cacatagaag ttaaacggtt acattgattt cagattgtta ctgattcgat caggcaaaac 1980 cttaattaat gagatggatt tatatattat ttatccgtat tattagttgt tttttttctt 2040 ttgaaatact tatagactgg ggggatatag tatttatacc ataaagaaaa tttttaataa 2100 taattcagtt ttagctttag attcagaaaa aagagagatt gtcattttag gatgtggaat 2160 agcatttaaa aagaaagtaa atgacaaagt ttcagaagat aacgttgaaa aaacatttat 2220 acttaaacaa aaggatgctt cagaaaaatt taaattattg ttagaagata tttcagcaga 2280 gtatatatct ctatgttatg acataataga atatgctaaa aatattcttg ataaagagct 2340 taatgattac atatatgtaa cccttacaga tcatataaat tatgttattg aagcttgtaa 2400 acagggtata tctaaaccca atatattaat atgggaaata aaaaaattct atccaaaaga 2460 atttgccgtt ggattaaaag ctatagagtt tattgaagat gaattagggt acaagcttca 2520 cgaagatgaa gctggaaata tcgcacttca tctaataaat gctcaagtta atgaaaaatc 2580 tgatacagtt gaagatgtat ataatattac aaagaagata aatgatattc ttaatatagt 2640 taaatataca tacaatcttg aattagatga aaaaacttta aattatgaaa gatttataac 2700 tcatctaaga ttcttcttta aacgacttga aagaaaagaa attacagaat ctgaagataa 2760 cttcttatta gaacaagtaa aaaataaata tacagatgct tatgaatgta tgcttaagat 2820 tgaaaaatac ttgggtcagg aattaagtaa tgaagaacag ttatatttaa tgttacatat 2880 tcaacgtgta acaacaagag aaaagtaaaa aataggattg ttactgataa tgcaggcaag 2940 accgtaaaat ttagaggaaa tttatattat aaattttgct ttaaatatta tgtgtcttgc 3000 ctttttatat taatcattct acgaggagag atgaactatg aaatatgaac aattagctaa 3060 agacatatta aaaaatgtcg gtggcaaaga aaacgttaac agcttaacac attgcattac 3120 tagattaaga tttaaattaa aagatgaagg taaagcaaat acagatatat taaaaaatat 3180 ggatggagta gttaccgtag ttaaaagcgg tggacaatat caagtagtta taggaaatca 3240 tgttccagac gtatttgcag cagttaatgc tgtaggggga cttcaaggag cttcagaaga 3300 agatgaaact gaagaaaaga tgaatccttt aaataaattt atagatattg tttctggtgt 3360 attccaacca atacttggag ttttatgtgc tacaggtatg attaaaggtt taaatgcagt 3420 attagttgca gcaggagttt tagctagtac agattctact tacataatac taaatgctat 3480 tggtgattgt ttatttaact tcttcccaat attcctaggt tttacagcag ctaaaaagtt 3540 taagttaaac caatttacag gtatggcagt tggagcagct atggtttatc cagcaataac 3600 ttcatttgca ggtcaaacag ttgatttctt tggtattcca gtagtaatgc cttcaagcag 3660 ttatcaatca acagttattc ctataatact agctatatat atagcatcaa aggttgaaaa 3720 attatttaag aagattatac ctgatatggt taaaacattc ttagttccat ttgcaacatt 3780 actagtagta gttccagtta cttttatggc aataggtccc atatcaacta tagcagctaa 3840 cgctttagga gatttaacat tagcaattta taactttaat ccaacaatcg caggtttatt 3900 tattggtgga ttctggcaag tattcgttat gtttggttta cactggggat tagttccaat 3960 agcaatgaac aacttagcag taattggtta tgatccggtt ttagcaactt cagttgcagt 4020 atgtttcgct caaacaggtg ttgtaatggc aatacttgca aaaactaaag ataaaaagtt 4080 aaagtcacta tgtattccag caattatttc aggattcttt ggagttacag aaccagctat 4140 ctacggtatt acattaccaa gaaagaaacc atttatatta agctgtattg ctgcaggtgt 4200 tactggtgga atcataggat tctttgaaag taaaggttat tcaatgggtg gtcttggaat 4260 atttgctctt ccaagctata taaatccaga aggaattgat agaggtttct atggtatggt 4320 aatagctatg gtagctggta tagtagtagg attcatatta atgtttgtaa ctaaacttaa 4380 tgatgaagaa gaagtaaaga caacagaaag taaaaaagaa gaatcattag ttaaacaaga 4440 agaaatagta agcccaattc aaggtgaagt agttacttta gcagaagtta aagatgaagc 4500 attctcatca ggagctttag gaaaaggtgt tgcaatcaac cctatagaag gaaaagttta 4560 tgcaccagct gatggaacat taacaacttt attcccatca ctacatgcac ttggtataac 4620 tacagaaaat ggtgcagaaa tattaataca cgttggtatg gacactgttc aattagaagg 4680 aaaacacttt actgcaaaag ttaaacaagg tgataagatc aaaaagggac aattattaat 4740 agaatttgat aaagaagcaa tagaaaaagc agggtattca acaattactc cagtgctaat 4800 tacaaattca gatcaatatt tagatgtaat agaaactgat aaaagaaaag tagatgttaa 4860 ttcagaatta ttaacagtag taatataaga cagttaaaga aaggcggaat ttaaaatgtc 4920 atttgattta agttttccaa aaagtttttt atggggagga gctacagctg caaaccaatt 4980 tgagggagcg tataatgaag atggaaaggg cttatctata caagatatag caccaaaagg 5040 agtaatggga cctataacag aggtcccaac agaagataat atgaaactta ttgggataga 5100 tttttatcac agatataaag aagatataaa gctttttgca gaaatgggct ttaaaacttt 5160 tagattatct atagcttggt caagaatatt cccaaatggt gatgatgaga tacctaatga 5220 aaaaggtttg gaattttatg acaaagtatt tgatgaactt caaaggtatg gaattgaacc 5280 actagtaact ttatctcact atgaaactcc acttaattta tctaagaaat ataatggatg 5340 ggcaaataga gatcttatag gcttctatga aagatatgta agaactgtat tcactagata 5400 taaagataag gtaaaatact ggcttacttt taatgaaata aactcagcaa tacatgctcc 5460 atatatgagt gcaggaattt ggactgataa gtcagaatta agtaaacaag atttatatca 5520 agcaatgcat catgaattag ttgcaagcgc cttagcagta aaaattggac atgaaattaa 5580 ccctgatttt aaaataggat gtatgattct tggaattcct gtatatccat taacaccaca 5640 tcctgatgat ttaattgaaa aaatgagagt ggaaagagaa agtctattct ttgcagatgt 5700 acatgctaga ggaaaatatc caagatatat gaatagatta tttaaggaaa ataatataga 5760 aataaaatgg catgaagatg atgcagagat tttaagtaat gtagtagatt ttatatcatt 5820 tagctattac atgagctcat gtgcaacagc agatgaagaa aagaagaaag caggagctgg 5880 caatttatta gctggagtac ctaatccata tttaaaagca tctgaatggg gatggcaaat 5940 tgatcctaag ggacttagat taatacttaa tgaattatat gatagatatg aaaagccatt 6000 atttatagtt gaaaatggtc ttggagctgt agatgaactt gttactgatg aaaatggtaa 6060 taaaacagta aatgatgact atagaattaa gtatttaaat gaccatcttg ttcaagtagc 6120 agaagctata gaagatggag ttgaacttat gggatataca acttggggat gtattgactt 6180 agtttcagct tcaacagcag aactgaagaa gagatatgga tttatctatg ttgatagaca 6240 tgatgatggc tcaggaactt tagaaagata taaaaagaag agctttaatt ggtacaaaga 6300 agtaattgct actaatggaa agagcttaga aagataatta ttaataaata tagaaatgaa 6360 tctgaaaagt taaaaattca gattcatttc tttttgtgtt tagaaaagat ataaagatga 6420 aataattaga aactaggagg gatttatgga tatagaaaaa gttataaaat ttatagataa 6480 aagtatagat gaaaaagaaa attataatga gcttattata aaggaattaa ttaaagataa 6540 gaatgggtta attcctcttt tagttaaaat tgatatattt tattttttgt atatactcaa 6600 agttttagat gaagtgattt taatactata tagtaaagat tttataagtg agtttaaaag 6660 aaaaatatac ataaggtatg ataaactttc aaatataaat gtaaataacc atgaaacaat 6720 aaaagataaa tgtaataatt aatatataaa ggtttttgta atgatttgaa aatataacta 6780 agttaaatca tataaataag ctataatata aagtaagaaa attaaataag atttatggga 6840 ttaggaagtg tatcgttatg aagaaaattg aagctgtaat acgtccggaa aaacttgaag 6900 aattaaagga tgctttaaaa aaaggcaata taaacggaat tactgtagat caagtaatgg 6960 gatgtggtca gcaacatgga tggactgaac attatagagg aagcgaaatc atggtgaact 7020 ttttacctaa ggttgaagta aaggttatag ttgatgactc aaaggtgcaa gaggtcataa 7080 atttaattat tgatacagta aaaacagggg aagttggaga cggaaaaata tttgttagtg 7140 atgttagtga atgcataaga ataaggacag gagaaaaagg attagatgca ttataaaaga 7200 atttattatt aaaaaatatt actggaaatt tagttttcca gtaatatttt tttaaagtct 7260 aaattttttc tttttatact ttttgaaagt tttaaatagt gcagatataa atattatccc 7320 aagagctagt gcaccagcat tgtataaagt agtaactcca taatctatgg cactaatata 7380 atctccaact aagaaagcat acatacattt atatacccca tatccaggaa caacaggaat 7440 aagagcacac acacttaata aagttacagg ggtattaaat tttcttgcac aaatttcact 7500 atataaacta aaggctgc 7518 // |
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