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EBI DbfetchID L41499; SV 1; linear; genomic DNA; STD; PRO; 5417 BP. XX AC L41499; XX DT 21-APR-1995 (Rel. 43, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Staphylococcus aureus ORF1, partial cds, ORF2, ORF3, autolysin (atl) genes, DE complete cds. XX KW autolysin; peptidoglycan hydrolase. XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-5417 RX PUBMED; 7559367. RA Foster S.J.; RT "Molecular characterization and functional analysis of the major autolysin RT of Staphylococcus aureus 8325/4"; RL J. Bacteriol. 177(19):5723-5725(1995). XX FH Key Location/Qualifiers FH FT source 1..5417 FT /organism="Staphylococcus aureus" FT /strain="8325/4" FT /mol_type="genomic DNA" FT /db_xref="taxon:1280" FT CDS <1..249 FT /codon_start=1 FT /transl_table=11 FT /note="ORF1" FT /db_xref="InterPro:IPR004474" FT /db_xref="UniProtKB/Swiss-Prot:P52078" FT /protein_id="AAA99979.1" FT /translation="DQRVINHYGSLIPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNN FT SDSSNQQQPATDQNSNQNQGGTQQAPQASNNQNGVVN" FT RBS 281..288 FT CDS 297..767 FT /codon_start=1 FT /transl_table=11 FT /note="ORF2" FT /db_xref="UniProtKB/Swiss-Prot:P0A0Q4" FT /protein_id="AAA99980.1" FT /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHRE FT NYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMKSL FT SIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL" FT CDS 922..1356 FT /codon_start=1 FT /transl_table=11 FT /note="ORF3" FT /db_xref="GOA:P0A0M9" FT /db_xref="InterPro:IPR000182" FT /db_xref="InterPro:IPR016181" FT /db_xref="UniProtKB/Swiss-Prot:P0A0M9" FT /protein_id="AAA99981.1" FT /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHLI FT GYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVATM FT NAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN" FT RBS 1568..1572 FT CDS 1584..5354 FT /codon_start=1 FT /transl_table=11 FT /gene="atl" FT /product="autolysin" FT /note="ORF4" FT /db_xref="GOA:Q2FZK7" FT /db_xref="InterPro:IPR002502" FT /db_xref="InterPro:IPR002901" FT /db_xref="InterPro:IPR013338" FT /db_xref="UniProtKB/Swiss-Prot:Q2FZK7" FT /protein_id="AAA99982.1" FT /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDS FT NKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGD FT TRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKSAAK FT PAALETQYKTAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVN FT SSINDYICKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYM FT KNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNN FT YADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQL FT YDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNS FT GLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTA FT KSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSV FT NGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYD FT KTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQ FT TYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVS FT KAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKN FT GAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTV FT NKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKD FT LTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVI FT NGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGK FT WTDAKFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAF FT NKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDN FT DPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATD FT VDWANINAKIIKGYYDKIGEVGKYFDIPQYK" FT repeat_region 2856..3348 FT /gene="atl" FT /rpt_type=DIRECT FT /note="imperfect" FT repeat_region 3369..3855 FT /gene="atl" FT /rpt_type=DIRECT FT /note="imperfect" FT repeat_region 3957..4377 FT /gene="atl" FT /rpt_type=DIRECT FT /note="imperfect" XX SQ Sequence 5417 BP; 2087 A; 922 C; 898 G; 1510 T; 0 other; gatcaacgtg tcattaatca ttatggttca ttaataccat taacgccttt agataatagt 60 ttattgagaa aagaacaaaa tgatacgaca gataaagata agacatctaa cgagaacagt 120 gattcaacta ataacagtga ttccagcaat caacaacaac ctgctacaga tcaaaactca 180 aatcaaaatc aaggtggcac acaacaagcg ccacaagctt caaataacca aaatggtgtt 240 gtaaattaat attatatgac gattaacatt tactatttat gaaaggagca tcatacatga 300 gtagaaaaac atacgaaaag attgcaaata taaatggcat gtttaatatg ttagaacaac 360 aaatcattca tagccaagat atggctcatt ttagaagtga atttttttac gtcaatcatg 420 agcatcgaga aaactatgaa gcactcctaa tttattacaa aaatagtatc gacaatccta 480 ttgtagatgg tgcatgttat attttagccc tacctgaaat tttcaatagt gttgatgttt 540 tcgaatcaga gttaccattt tcatgggtat atgatgaaaa tggcattacc gaaacaatga 600 aatcacttag cattccatta caatatttag ttgcagcagc tttagaagta actgatgtga 660 atatatttaa gccttcagga tttacaatgg gaatgaataa ttggaatatt gctcaaatgc 720 gaatcttttg gcaatataca gcaattatta gaaaagaagc actataacat taataattaa 780 ttagctataa agatgattca caacaatcat ctttatagct tttttatgtc taattatttt 840 tgaggaaaat aacaatcgtt attctatgtt attattagta aattaaatgt aaatgatttt 900 gttaggatgt ggaggaattt tatgttttca aaagtaaaca atcaaaagat gttagaagat 960 tgcttctata taagaaagaa agtgtttgta gaagaacaag gcgtccctga ggaaagtgaa 1020 attgatgaat atgaatctga atctattcac ctcattggat atgataatgg acagccagtt 1080 gccactgctc gaatacgccc tattaatgaa acaactgtca aaatagaacg agtagctgtg 1140 atgaaatcac atcgtggaca aggaatgggt agaatgctta tgcaagctgt agaatcatta 1200 gctaaagatg aaggttttta cgtagctact atgaatgccc aatgtcatgc tatcccattt 1260 tatgaaagtt taaactttaa aatgagaggt aatatatttc ttgaggaagg catcgagcat 1320 attgaaatga caaaaaagtt aacctcgctt aattaaaaaa agttgtatct attttagaaa 1380 catttgtaat aaaccaattc ttaaattgac atcaattcta tttgatttgt cacgtcacca 1440 ttgagattca aaattttttt cattttttta cagtgaaaat gtaaattaag agtatattac 1500 aaattggtta aatacgcaca ggtatataaa acaggtacta taatgttagt aataattaat 1560 aaatgttagg agtaataaat agaatggcga aaaaattcaa ttacaaacta ccatcaatgg 1620 ttgcattaac gcttgtaggt tcagcagtca ctgcacatca agttcaagca gctgagacga 1680 cacaagatca aactactaat aaaaacgttt tagatagtaa taaagttaaa gcaactactg 1740 aacaagcaaa agctgaggta aaaaatccaa cgcaaaacat ttctggcact caagtatatc 1800 aagaccctgc tattgtccaa ccaaaaacag caaataacaa aacaggcaat gctcaagtaa 1860 gtcaaaaagt tgatactgca caagtaaatg gtgacactcg tgctaatcaa tcagcgacta 1920 caaataatac gcagcctgtt gcaaagtcaa caagcactac agcacctaaa actaacacta 1980 atgttacaaa tgctggttat agtttagttg atgatgaaga tgataattca gaaaatcaaa 2040 ttaatccaga attaattaaa tcagctgcta aacctgcagc tcttgaaacg caatataaaa 2100 ccgcagcacc taaagctgca actacatcag cacctaaagc taaaactgaa gcgacaccta 2160 aagtaactac ttttagcgct tcagcacaac caagatcagt tgctgcaaca ccaaaaacga 2220 gtttgccaaa atataaacca caagtaaact cttcaattaa cgattacatt tgtaaaaata 2280 acttaaaagc acctaaaatt gaagaagatt atacatctta cttccctaaa tacgcatacc 2340 gtaacggcgt aggtcgtcct gaaggtatcg tagttcatga tacagctaat gatcgttcga 2400 cgataaatgg tgaaattagt tatatgaaaa ataactatca aaacgcattc gtacatgcat 2460 ttgttgatgg ggatcgtata atcgaaacag caccaacgga ttacttatct tggggtgtcg 2520 gtgcagtcgg taaccctaga ttcatcaatg ttgaaatcgt acacacacac gactatgctt 2580 catttgcacg ttcaatgaat aactatgctg actatgcagc tacacaatta caatattatg 2640 gtttaaaacc agacagtgct gagtatgatg gaaatggtac agtatggact cactacgctg 2700 taagtaaata tttaggtggt actgaccatg ccgatccaca tggatattta agaagtcata 2760 attatagtta tgatcaatta tatgacttaa ttaatgaaaa atatttaata aaaatgggta 2820 aagtggcgcc atggggtacg caatctacaa ctacccctac tacaccatca aaaccaacaa 2880 caccgtcgaa accatcaact ggtaaattaa cagttgctgc aaacaatggt gtcgcacaaa 2940 tcaaaccaac aaatagtggt ttatatacta ctgtatacga caaaactggt aaagcaacta 3000 atgaagttca aaaaacattt gctgtatcta aaacagctac attaggtaat caaaaattct 3060 atcttgttca agattacaat tctggtaata aatttggttg ggttaaagaa ggcgatgtgg 3120 tttacaacac agctaaatca cctgtaaatg taaatcaatc atattcaatc aaacctggta 3180 cgaaacttta tacagtacct tggggtacat ctaaacaagt tgctggtagt gtgtctggct 3240 ctggaaacca aacatttaag gcttcaaagc aacaacaaat tgataaatca atttatttat 3300 atggctctgt gaatggtaaa tctggttggg taagtaaagc atatttagtt gatactgcta 3360 aacctacgcc tacaccaaca cctaagccat caacacctac aacaaataat aaattaacag 3420 tttcatcatt aaacggtgtt gctcaaatta atgctaaaaa caatggctta ttcactacag 3480 tttatgacaa aactggtaag ccaacgaaag aagttcaaaa aacatttgct gtaacaaaag 3540 aagcaagttt aggtggaaac aaattctact tagttaaaga ttacaatagt ccaactttaa 3600 ttggttgggt taaacaaggt gacgttattt ataacaatgc aaaatcacct gtaaatgtaa 3660 tgcaaacata tacagtaaaa ccaggcacta aattatattc agtaccttgg ggcacttata 3720 aacaagaagc tggtgcagtt tctggtacag gtaaccaaac ttttaaagcg actaagcaac 3780 aacaaattga taaatctatc tatttatttg gaactgtaaa tggtaaatct ggttgggtaa 3840 gtaaagcata tttagctgta cctgctgcac ctaaaaaagc agtagcacaa ccaaaaacag 3900 ctgtaaaagc ttatactgtt actaaaccac aaacgactca aacagttagc aagattgctc 3960 aagttaaacc aaacaacact ggtattcgtg cttctgttta tgaaaaaaca gcgaaaaacg 4020 gtgcgaaata tgcagaccgt acgttctatg taacaaaaga gcgtgctcat ggtaatgaaa 4080 cgtatgtatt attaaacaat acaagccata acatcccatt aggttggttc aatgtaaaag 4140 acttaaatgt tcaaaactta ggcaaagaag ttaaaacgac tcaaaaatat actgttaata 4200 aatcaaataa cggcttatca atggttcctt ggggtactaa aaaccaagtc attttaacag 4260 gcaataacat tgctcaaggt acatttaatg caacgaaaca agtatctgta ggcaaagatg 4320 tttatttata cggtactatt aataaccgca ctggttgggt aaatgcaaaa gatttaactg 4380 caccaactgc tgtgaaacca actacatcag ctgccaaaga ttataactac acttatgtaa 4440 ttaaaaatgg taatggttat tactatgtaa caccaaattc tgatacagct aaatactcat 4500 taaaagcatt taatgaacaa ccattcgcag ttgttaaaga acaagtcatt aatggacaaa 4560 cttggtacta tggtaaatta tctaacggta aattagcatg gattaaatca actgatttag 4620 ctaaagaatt aattaagtat aatcaaacag gtatgacatt aaaccaagtt gctcaaatac 4680 aagctggttt acaatataaa ccacaagtac aacgtgtacc aggtaagtgg acagatgcta 4740 aatttaatga tgttaagcat gcaatggata cgaagcgttt agctcaagat ccagcattaa 4800 aatatcaatt cttacgctta gaccaaccac aaaatatttc tattgataaa attaatcaat 4860 tcttaaaagg taaaggtgta ttagaaaacc aaggtgctgc atttaacaaa gctgctcaaa 4920 tgtatggcat taatgaagtt tatcttatct cacatgccct attagaaaca ggtaacggta 4980 cttctcaatt agcgaaaggt gcagatgtag tgaacaacaa agttgtaact aactcaaaca 5040 cgaaatacca taacgtattt ggtattgctg catatgataa cgatccttta cgtgaaggta 5100 ttaaatatgc taaacaagct ggttgggaca cagtatcaaa agcaatcgtt ggtggtgcta 5160 aattcatcgg caactcatat gtaaaagctg gtcaaaatac actttacaaa atgagatgga 5220 atcctgcaca tccaggaaca caccaatatg ctacagatgt agattgggct aacatcaatg 5280 ctaaaatcat caaaggctac tatgataaaa ttggcgaagt cggcaaatac ttcgacatcc 5340 cacaatataa ataagcaaca tgaacatagg atcaaaagtc atcccccact atcaatcatg 5400 ggggatgacc tttgatc 5417 // |
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