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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   L40408; SV 1; linear; genomic DNA; STD; PRO; 1059 BP.
XX
AC   L40408;
XX
DT   28-MAR-1995 (Rel. 43, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
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DE   Nocardioides sp. (strain N106) chitosanase (csn) gene, complete cds.
XX
KW   chitosanase.
XX
OS   Nocardioides sp.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Propionibacterineae; Nocardioidaceae; Nocardioides.
XX
RN   [1]
RP   1-1059
RA   Masson J.-Y., Boucher I., Neugebauer W.A., Ramotkar D., Brzezinski R.;
RT   "Cloning and sequencing of the chitosanase gene from the actinomycete
RT   Nocardioides sp. N106 and characterization of the gene product";
RL   Microbiology (Reading, Engl.) 0:0-0(1995).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1059
FT                   /organism="Nocardioides sp."
FT                   /strain="N106"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:35761"
FT   repeat_region   53..73
FT                   /function="putative binding site for a transcription
FT                   factor"
FT                   /rpt_type=INVERTED
FT                   /note="homologous to an inverted repeat sequence upstream
FT                   the coding sequence of the chitosanase from Streptomyces
FT                   N174; putative"
FT   sig_peptide     128..250
FT                   /gene="csn"
FT   CDS             128..964
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="csn"
FT                   /product="chitosanase"
FT                   /function="enzyme; glycosyl hydrolase family 46"
FT                   /EC_number="3.2.1.132"
FT                   /db_xref="GOA:P48846"
FT                   /db_xref="HSSP:1CHK"
FT                   /db_xref="InterPro:IPR000400"
FT                   /db_xref="UniProtKB/Swiss-Prot:P48846"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAA63405.1"
FT                   /translation="MRLKHPTARLALAALLVAVPRSVAAAGTVHAAPAPAGATRLAAVG
FT                   LDDPHKKDIAMQLVSSAENSSLDWKSQYKYIEDIKDGRGYTAGIIGFCSGTGDMLDLVA
FT                   DYTDLKPGNILAKYLPALRKVNGTESHAGLASAFEKDWATAAKDSVFQQAQNDERDRSY
FT                   FNPAVNQAKASLRALGQFAYYDAIVMHGPGDSSDSFGGIRKAAMKKAKTPAQGRDEATY
FT                   LKAFLAARKTVMLKEEAHSDTSRVDTEQTVFLNAKNFDLNPPLKWKVYGDSYAINS"
FT   mat_peptide     251..961
FT                   /gene="csn"
FT                   /product="chitosanase"
FT                   /EC_number="3.2.1.132"
XX
SQ   Sequence 1059 BP; 199 A; 405 C; 322 G; 133 T; 0 other;
     gcaaacatgg cgccagggcc ttgcgcggtg gtgggcgtga acgcttcaat ctagttagga        60
     aactttccta actctcctca tgggtccgga gacccccatg ccccggacca gggaaacgga       120
     gcgccccatg cgtctcaagc accccaccgc ccggctcgcc ctcgccgccc tgctggtcgc       180
     cgtcccgcgc agtgtcgcgg ccgccggcac cgtccacgcc gccccggccc ccgccggcgc       240
     cacccggctc gcggccgtcg ggctggacga cccgcacaag aaggacatcg ccatgcagct       300
     ggtgtccagc gccgagaact cctccctcga ctggaagtcc cagtacaagt acatcgagga       360
     catcaaggac ggccgcggct acaccgccgg catcatcggc ttctgttccg gcaccggcga       420
     catgctcgac ctggtcgcag actacaccga cctcaagccg ggcaacatcc tcgccaagta       480
     cctcccggcg ctgcgcaagg tcaacggcac cgagtcgcac gccggcctcg cgtccgcctt       540
     cgagaaggac tgggccaccg ccgccaagga cagcgtcttc cagcaggccc agaacgacga       600
     gcgcgaccgc tcgtacttca accccgcggt caaccaggcc aaggcgagcc tgcgcgcgct       660
     cggccagttc gcctactacg acgcgatcgt catgcacggc cccggcgaca gctcggacag       720
     cttcggcggc atccgcaagg ccgccatgaa gaaggccaag acccccgccc agggccgcga       780
     cgaggccacc tacctcaagg ccttcctggc ggcccgcaag accgtgatgc tgaaggagga       840
     ggcccactcg gacaccagcc gggtcgacac cgagcagacg gtgttcctca acgccaagaa       900
     cttcgacctc aacccgccgc tgaagtggaa ggtctacggg gacagctacg ccatcaacag       960
     ctgacggtcc gccgggtggc cggaggcgcg gcggcggccg gcgctgccgt gtcccgaacg      1020
     cacgcacggc gcaccgtaat tcggatgacg gtcgaacgg                             1059
//
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