EBI Dbfetch

ID   L38902; SV 1; linear; genomic DNA; STD; PRO; 1296 BP.
XX
AC   L38902;
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DT   26-MAY-1995 (Rel. 43, Created)
DT   27-OCT-2009 (Rel. 102, Last updated, Version 7)
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DE   Pseudomonas fluorescens clone pROTM2 pectate lyase precursor (pel) gene,
DE   complete cds.
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KW   pectate lyase; pel gene.
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OS   Pseudomonas fluorescens
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RP   1-1296
RX   PUBMED; 1906062.
RA   Liao C.H.;
RT   "Cloning of pectate lyase gene pel from Pseudomonas fluorescens and
RT   detection of sequences homologous to pel in Pseudomonas viridiflava and
RT   Pseudomonas putida";
RL   J. Bacteriol. 173(14):4386-4393(1991).
XX
RN   [2]
RP   1-1296
RA   Bradley S.P.;
RT   "On the pectate lyase gene from soft-rot fluorescent Pseudomonads";
RL   Thesis (1995), University of Massachusetts at Lowell
XX
RN   [3]
RP   1-1296
RX   PUBMED; 8589419.
RA   Liao C.H., Gaffney T.D., Bradley S.P., Wong L.C.;
RT   "Cloning of a pectate lyase gene from Xanthomonas campestris pv.
RT   malvacearum and comparison of its sequence relationship with pel genes of
RT   soft-rot Erwinia and Pseudomonas";
RL   Mol. Plant Microbe Interact. 9(1):14-21(1996).
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RN   [4]
RP   1-1296
RA   Gaffney T.B., Liao C.H.;
RT   ;
RL   Submitted (01-JAN-1995) to the EMBL/GenBank/DDBJ databases.
RL   Biological Science, University of Massachusetts-Lowell, 1 University
RL   Avenue, Lowell, MA 01854, USA
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FH   Key             Location/Qualifiers
FH
FT   source          1..1296
FT                   /organism="Pseudomonas fluorescens"
FT                   /strain="CY091"
FT                   /sub_strain="Biovar 2"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="C.H. Liao (USDA)"
FT                   /clone="pROTM2"
FT                   /db_xref="taxon:294"
FT   misc_binding    4..27
FT                   /function="regulation"
FT                   /bound_moiety="unknown"
FT                   /note="5' UTR binding site; regulatory binding site"
FT                   /citation=[2]
FT                   /citation=[4]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   RBS             45..49
FT                   /note="potential Shine-Dalgarno sequence"
FT                   /citation=[4]
FT                   /citation=[1]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   gene            64..1206
FT                   /gene="pel"
FT   CDS             64..1206
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="pel"
FT                   /product="pectate lyase precursor"
FT                   /function="trans-eliminative cleavage of poly-pectate"
FT                   /EC_number="4.2.2.2"
FT                   /db_xref="GOA:Q59671"
FT                   /db_xref="InterPro:IPR002022"
FT                   /db_xref="InterPro:IPR006626"
FT                   /db_xref="InterPro:IPR011050"
FT                   /db_xref="InterPro:IPR012334"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q59671"
FT                   /citation=[4]
FT                   /citation=[1]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAB46399.1"
FT                   /translation="MTNPSTFTASKLASAVIGALLLSSVPAHAADIWLDSATTGWATQN
FT                   GGTKGGSRAAANNIYTAKNAAELKTALKASVGANGRIIKITGIIDISEGKAYTTTADMK
FT                   VRGRLDIPGKTTIVGTTSNAEIREGFLYAKENDVIIRNITIENPWDPEPKWDPTDGSAG
FT                   NWNSEYDGLTIEGANNVWVDHVTFTDGRRTDDQNGTANGRPKQHHDGALDVKNGANYVT
FT                   ISYSAFKSHEKNNLIGSSDSRTTDDGKLKVTIHNTLFENISARAPRVRFGQVHLYNNYH
FT                   VGSTSHKVYPFSYAHGMGKNSKIFSERNAFEISGISGCDKIAGDYGGNVYRDTGSTVNG
FT                   TALTCPWSTNIGWTPPYSYTPLAADKVAADVKAKAGAGKL"
FT   sig_peptide     64..147
FT                   /gene="pel"
FT                   /function="translocation"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   mat_peptide     148..1203
FT                   /gene="pel"
FT                   /product="pectate lyase"
FT                   /function="trans-eliminative cleavage of poly-pectate"
FT                   /EC_number="4.2.2.2"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   terminator      1232..1253
FT                   /standard_name="Rho-independent transcription terminator"
FT                   /citation=[2]
FT                   /citation=[1]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
XX
SQ   Sequence 1296 BP; 312 A; 410 C; 345 G; 229 T; 0 other;
     gtcgagaaac gcgcatgcct gtgcgcgttt aaacactcaa gttaatcaag gatctgatta        60
     ctcatgacaa acccctcaac cttcactgcc tccaaactgg ccagtgccgt tatcggtgcc       120
     ctgctgttgt ccagcgttcc tgcgcatgct gcggacattt ggctggattc agccaccacc       180
     ggctgggcca ctcaaaacgg cggcaccaag ggcggctcac gagctgcggc gaataatatc       240
     tacacggcaa aaaacgcggc tgagttgaaa accgcgctga aggcttcggt gggagccaac       300
     ggccgcatca tcaaaatcac cggcatcatc gatatcagcg aaggcaaggc ttatacgacc       360
     actgctgata tgaaggtacg cggtcgcctg gatattcctg gcaaaaccac catcgtcggt       420
     accaccagca atgccgaaat ccgcgagggt ttcctctatg ccaaggaaaa cgacgtcatc       480
     atccgcaaca tcaccatcga aaacccttgg gatcccgagc cgaagtggga ccctactgac       540
     ggcagcgccg gcaactggaa ctcggaatac gacggcctga ccattgaagg cgccaacaac       600
     gtgtgggtcg atcacgtgac cttcaccgat ggccgccgta ccgatgacca gaacggcacc       660
     gccaacggcc gtccgaagca gcatcacgac ggcgcgctgg atgttaaaaa cggcgccaac       720
     tacgtgacca tctcgtactc ggcgttcaag tcccacgaga agaacaacct gatcggttcc       780
     agcgacagcc gcaccaccga tgacggcaag ctcaaggtca ccatccacaa caccctgttt       840
     gagaatatct ctgcccgtgc gccgcgcgtg cgtttcggcc aggtgcacct gtacaacaac       900
     taccatgtgg gcagcaccag ccataaggtg tacccgttct cgtacgccca tggcatgggc       960
     aagaactcga agatcttctc cgagcgcaat gccttcgaga tcagcggtat cagcggttgc      1020
     gacaagatcg ccggcgatta cggtggcaac gtctatcgcg acaccggctc gaccgtcaac      1080
     ggcactgcat tgacctgccc gtggagcacg aacatcggct ggaccccgcc gtacagctac      1140
     accccgctgg ctgccgataa ggtcgccgcc gacgtcaagg ccaaggccgg cgccggcaag      1200
     ctctaagcag caccgtcacc cgtgaaatga gaagccctcg gtgacgaggg ttttttgtgc      1260
     agctactgca ccagttggtt gatctcgatg atcggc                                1296
//