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EBI DbfetchID L37761; SV 1; linear; genomic DNA; STD; PRO; 5617 BP. XX AC L37761; XX DT 23-NOV-1994 (Rel. 41, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Acinetobacter sp. (strain RA3849) ORF, complete cds; beta-ketothiolase DE (phaA) gene, complete cds; acetoacetyl-CoA reductase (phaB) gene, complete DE cds; polyhydroxyalkanoic acid synthase (phaC) gene, complete cds. XX KW acetoacetyl-CoA reductase; beta-ketothiolase; KW polyhydroxyalkanoic acid synthase. XX OS Acinetobacter sp. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter. XX RN [1] RP 1-5617 RX PUBMED; 7635832. RA Schembri M.A., Bayly R.C., Davies J.K.; RT "Phosphate concentration regulates transcription of the Acinetobacter RT polyhydroxyalkanoic acid biosynthetic genes"; RL J. Bacteriol. 177(15):4501-4507(1995). XX FH Key Location/Qualifiers FH FT source 1..5617 FT /organism="Acinetobacter sp." FT /strain="RA3849" FT /mol_type="genomic DNA" FT /clone="pJKD1425" FT /db_xref="taxon:472" FT misc_signal 985..1102 FT /note="pho-box; putative" FT -10_signal 1013..1018 FT /gene="phaB" FT gene 1013..1831 FT /gene="phaB" FT RBS 1074..1077 FT /gene="phaB" FT CDS 1085..1831 FT /codon_start=1 FT /transl_table=11 FT /gene="phaB" FT /product="acetoacetyl-CoA reductase" FT /db_xref="GOA:P50203" FT /db_xref="InterPro:IPR002198" FT /db_xref="InterPro:IPR002347" FT /db_xref="InterPro:IPR011283" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P50203" FT /protein_id="AAA99472.1" FT /translation="MSEQKVALVTGALGGIGSEICRQLVTAGYKIIATVVPREEDREKQ FT WLQSEGFQDSDVRFVLTDLNNHEAATAAIQEAIAAEGRVDVLVNNAGITRDATFKKMSY FT EQWSQVIDTNLKTLFTVTQPVFNKMLEQKSGRIVNISSVNGLKGQFGQANYSASKAGII FT GFTKALAQEGARSNICVNVVAPGYTATPMVTAMREDVIKSIEAQIPLQRLAAPAEIAAA FT VMYLVSEHGAYVTGETLSINGGLYMH" FT misc_signal 1823..1841 FT /note="pho-box; putative" FT -10_signal 1848..1853 FT RBS 1871..1874 FT CDS 1882..2226 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="ORF" FT /db_xref="InterPro:IPR014176" FT /db_xref="InterPro:IPR018968" FT /db_xref="UniProtKB/TrEMBL:Q44304" FT /protein_id="AAA99473.1" FT /translation="MLYGDLFSNMNAQYKNVFEPYTKFNSLVAKNFADLTNLQLEAARN FT YANIGLAQMFANSEVKDMQSMVNCTTKQLETMNKLSQQMIEDGKKLATLTTEFKSEFEK FT LVSESMPNNK" FT RBS 2340..2343 FT gene 2351..4123 FT /gene="phaC" FT CDS 2351..4123 FT /codon_start=1 FT /transl_table=11 FT /gene="phaC" FT /product="polyhydroxyalkanoic acid synthase" FT /db_xref="GOA:Q57164" FT /db_xref="InterPro:IPR000073" FT /db_xref="InterPro:IPR010941" FT /db_xref="InterPro:IPR010963" FT /db_xref="UniProtKB/TrEMBL:Q57164" FT /protein_id="AAA99474.1" FT /translation="MNPNSFQFKENILQFFSVHDDIWKKLQEFYYGQSPINEALAQLNK FT EDMSLFFEALSKNPARMMEMQWSWWQGQIQIYQNVLMRSVAKDVAPFIQPESGDRRFNS FT PLWQEHPNFDLLSQSYLLFSQLVQNMVDVVEGVPDKVRYRIHFFTRQMINALSPSNFLW FT TNPEVIQQTVAEQGENLVRGMQVFHDDVMNSGKYLSIRMVNSDSFSLGKDLAYTPGAVV FT FENDIFQLLQYEATTENVYQTPILVVPPFINKYYVLDLREQNSLVNWLRQQGHTVFLMS FT WRNPNAEQKELTFADLITQGSVEALRVIEEITGEKEANCIGYCIGGTLLAATQAYYVAK FT RLKNHVKSATYMATIIDFENPGSLGVFINEPVVSGLENLNNQLGYFDGRQLAVTFSLLR FT ENTLYWNYYIDNYLKGKEPSDFDILYWNSDGTNIPAKIHNFLLRNLYLNNELISPNAVK FT VNGVGLNLSRVKTPSFFIATQEDHIALWDTCFRGADYLGGESTLVLGESGHVAGIVNPP FT SRNKYGCYTNAAKFENTKQWLDGAEYHPESWWLRWQAWVTPYTGEQVPARNLGNAQYPS FT IEAAPGRYVLVNLF" FT -35_signal 4132..4137 FT -10_signal 4156..4161 FT RBS 4195..4199 FT /gene="phaA" FT gene 4195..5384 FT /gene="phaA" FT CDS 4206..5384 FT /codon_start=1 FT /transl_table=11 FT /gene="phaA" FT /product="beta-ketothiolase" FT /db_xref="GOA:Q44305" FT /db_xref="HSSP:1DM3" FT /db_xref="InterPro:IPR002155" FT /db_xref="InterPro:IPR016038" FT /db_xref="InterPro:IPR016039" FT /db_xref="InterPro:IPR020610" FT /db_xref="InterPro:IPR020613" FT /db_xref="InterPro:IPR020615" FT /db_xref="InterPro:IPR020616" FT /db_xref="InterPro:IPR020617" FT /db_xref="UniProtKB/TrEMBL:Q44305" FT /protein_id="AAA99475.1" FT /translation="MKDVVIVAAKRTAIGSFLGSLASLSAPQLGQTAIRAVLDSANVKP FT EQVDQVIMGNVLTTGVGQNPARQAAIAAGIPVQVPASTLNVVCGSGLRAVHLAAQAIQC FT DEADIVVAGGQESMSQSAHYMQLRNGQKMGNAQLVDSMVADGLTDAYNQYQMGITAENI FT VEKLGLNREEQDQLALTSQQRAAAAQAAGKFKDEIAVVSIPQRKGEPVVFAEDEYIKAN FT TSLESLTKLRPAFKKDGSVTAGNASGINDGAAAVLMMSADKAAELGLKPLARIKGYAMS FT GIEPEIMGLGPVDAVKKTLNKAGWSLDQVDLIEANEAFAAQALGVAKELGLDLDKVNVN FT GGAIALGHPIGASGCRILVTLLHEMQRRDAKKGIATLCVGGGMGVALAVERD" XX SQ Sequence 5617 BP; 1737 A; 1107 C; 1187 G; 1586 T; 0 other; aagcttatag ctaacaccgc aatcaatttt ttcactcgtc tagcgtctgt caagcgcgta 60 ttttcaagat taaacccgcg tcctttgaga caactgaata aggtttcaat ttcccagcgt 120 aatgcataat cctgaatagc attggcatta aactgaggag aaacgacgag taaaagctct 180 ccattttcta actgtagtgc acttatatat agtttcaccc gaccaaccaa aatccgtcgt 240 ttacgacatt caatttgacc aactttaaga tggcgaaata aatcactaat tttatgattc 300 tttcctaaat gattggtgac aatgaagttt ttttaacacg aatgcagaag ttgatgtctt 360 ggttcaatta accatgtaaa ccactgctca ccgataaact ctctgtctgc gaacacattc 420 acaatacggt ctttaccaaa aatggctata aagcgttgaa tcaaagcaat acgctctttc 480 gtatctgaat ttccacgttt attaagcaat gtccaaagga taggtatcgc tattccacga 540 taaacgattg cgagcatcag gatattaata tttcgttttc cccatttcca attggttcta 600 tctaaagtca gttgcacttg gtcgaatgaa aacatattga aaatcaactg agaaatttga 660 cgataatcaa aatactgacc tgcaaagaag cgctgcatac gtcgataaaa tgattgtggt 720 aagcacttga tgggcaaggc tttagatgca gaagaaagat tacatgtttg ctttaaaata 780 atcacaagca tgatgagcgc aaagcacttt aaatgtgact tgttccattt tagatatttg 840 tttaagataa gatataactc attgagatgt gtcatagtat tcgtcgttag aaaacaatta 900 ttatgacatt atttcaatga gttatctatt tttgtcgtgt acagagcaat atttgtttac 960 ttttgacttt aaagcatcat caaactgcga tctgtttgca atataaaacg cttaatttct 1020 aaacaagaat aaagaggaaa aacttcttat ttttttataa ccttattctg cttaggaaaa 1080 caacatgtct gaacaaaaag tagctttagt gacgggtgca ttaggtggta ttggcagtga 1140 gatttgccgt caactggtta cagcaggcta taaaattatc gcaacggttg taccacgtga 1200 agaagaccgc gaaaaacaat ggcttcaaag tgaaggattt caagacagcg atgtacgctt 1260 tgtcttaacg gatttaaata accacgaagc ggcaacagca gctattcaag aagcaattgc 1320 tgctgaaggt cgtgtcgatg tgctggtcaa taatgcaggc atcacccgtg atgcaacttt 1380 taaaaagatg agctatgaac agtggtcaca agtcattgat accaacttaa aaacattgtt 1440 cacagtgact cagcccgtgt ttaacaaaat gttagaacaa aagtcgggac gtattgtcaa 1500 tatcagctca gtcaacggtt taaaaggtca gtttggacaa gccaactact ctgcgagcaa 1560 agccggcatt atcggtttta ccaaagcctt agctcaagaa ggtgcacgtt caaatatttg 1620 tgtaaacgtg gttgcgcctg gctataccgc aacaccaatg gttactgcca tgcgtgaaga 1680 tgtgattaaa agcattgaag cacaaattcc tctacaacgt cttgctgcgc cagctgaaat 1740 tgccgctgct gttatgtact tggtcagcga gcacggtgcg tacgtgacag gcgaaacctt 1800 atcgattaat ggcggtttat acatgcacta aaccgtgcag cccctatttt catttacaag 1860 tttatttact ggagttacac catgctatac ggcgacttat tttcaaatat gaatgcacaa 1920 tacaaaaacg tatttgaacc gtacacaaaa ttcaacagct tagtggctaa aaactttgct 1980 gacttaacca acctacaatt agaagcagca cgcaactatg ccaacattgg tctagcgcaa 2040 atgtttgcca atagtgaagt taaagacatg caaagcatgg tgaattgcac caccaagcaa 2100 ttagaaacca tgaacaaact tagtcagcaa atgattgaag atggcaaaaa gttggcaaca 2160 ctaacgactg aattcaaatc ggaatttgaa aagttagtta gcgaatctat gcctaacaat 2220 aaataacact gctctgaaaa ccatgcgtta tcaggacgaa tgttacgggg aagtgtgaaa 2280 atttccccgt tttagtttca gccctgcact caatttgatt gctaaaagcc atgtgctatg 2340 gagcgatgaa atgaacccga actcatttca attcaaagaa aacatactac aatttttttc 2400 tgtacatgat gacatctgga aaaaattaca agaattttat tatgggcaaa gcccaattaa 2460 tgaggctttg gcgcagctca acaaagaaga tatgtctttg ttctttgaag cactatctaa 2520 aaacccagct cgcatgatgg aaatgcaatg gagctggtgg caaggtcaaa tacaaatcta 2580 ccaaaatgtg ttgatgcgca gcgtggccaa agatgtagca ccatttattc agcctgaaag 2640 tggtgatcgt cgttttaaca gcccattatg gcaagaacac ccaaattttg acttgttgtc 2700 acagtcttat ttactgttta gccagttagt gcaaaacatg gtagatgtgg tcgaaggtgt 2760 tccagacaaa gttcgctatc gtattcactt ctttacccgc caaatgatca atgcgttatc 2820 tccaagtaac tttctgtgga ctaacccaga agtgattcag caaactgtag ctgaacaagg 2880 tgaaaactta gtccgtggca tgcaagtttt ccatgatgat gtcatgaata gcggcaagta 2940 tttatctatt cgcatggtga atagcgactc tttcagcttg ggcaaagatt tagcttacac 3000 ccctggtgca gtcgtctttg aaaatgacat tttccaatta ttgcaatatg aagcaactac 3060 tgaaaatgtg tatcaaaccc ctattctagt cgtaccaccg tttatcaata aatattatgt 3120 gctggattta cgcgaacaaa actctttagt gaactggttg cgccagcaag gtcatacagt 3180 ctttttaatg tcatggcgta acccaaatgc cgaacagaaa gaattgactt ttgccgatct 3240 cattacacaa ggttcagtgg aagctttgcg tgtaattgaa gaaattaccg gtgaaaaaga 3300 ggccaactgc attggctact gtattggtgg tacgttactt gctgcgactc aagcctatta 3360 cgtggcaaaa cgcctgaaaa atcacgtaaa gtctgcgacc tatatggcca ccattatcga 3420 ctttgaaaac ccaggcagct taggtgtatt tattaatgaa cctgtagtga gcggtttaga 3480 aaacctgaac aatcaattgg gttatttcga tggtcgtcag ttggcagtta ccttcagttt 3540 actgcgtgaa aatacgctgt actggaatta ctacatcgac aactacttaa aaggtaaaga 3600 accttctgat tttgatattt tatattggaa cagcgatggt acgaatatcc ctgccaaaat 3660 tcataatttc ttattgcgca atttgtattt gaacaatgaa ttgatttcac caaatgccgt 3720 taaggttaac ggtgtgggct tgaatctatc tcgtgtaaaa acaccaagct tctttattgc 3780 gacgcaggaa gaccatatcg cactttggga tacttgtttc cgtggcgcag attacttggg 3840 tggtgaatca accttggttt taggtgaatc tggacacgta gcaggtattg tcaatcctcc 3900 aagccgtaat aaatacggtt gctacaccaa tgctgccaag tttgaaaata ccaaacaatg 3960 gctagatggc gcagaatatc accctgaatc ttggtggttg cgctggcagg catgggtcac 4020 accgtacact ggtgaacaag tccctgcccg caacttgggt aatgcgcagt atccaagcat 4080 tgaagcggca ccgggtcgct atgttttggt aaatttattc taatcggtca tataacaaca 4140 gccatgcaga tgctatatat catgtgcatc cacagaaaca tgaacacaaa atttaaggat 4200 ataaaatgaa agatgttgtg attgttgcag caaaacgtac tgcgattggt agctttttag 4260 gtagtcttgc atctttatct gcaccacagt tggggcaaac agcaattcgt gcagttttag 4320 acagcgctaa tgtaaaacct gaacaagttg atcaggtgat tatgggcaac gtactcacga 4380 caggcgtggg acaaaaccct gcacgtcagg cagcaattgc tgctggtatt ccagtacaag 4440 tgcctgcatc tacgctgaat gtcgtctgtg gttcaggttt gcgtgcggta catttggcag 4500 cacaagccat tcaatgcgat gaagccgaca ttgtggtcgc aggtggtcaa gaatctatgt 4560 cacaaagtgc gcactatatg cagctgcgta atgggcaaaa aatgggtaat gcacaattgg 4620 tggatagcat ggtggctgat ggtttaaccg atgcctataa ccagtatcaa atgggtatta 4680 ccgcagaaaa tattgtagaa aaactgggtt taaaccgtga agaacaagat caacttgcat 4740 tgacttcaca acaacgtgct gcggcagctc aggcagctgg caagtttaaa gatgaaattg 4800 ccgtagtcag cattccacaa cgtaaaggtg agcctgttgt atttgctgaa gatgaataca 4860 ttaaagccaa taccagcctt gaaagcctca caaaactacg cccagccttt aaaaaagatg 4920 gtagcgtaac cgcaggtaat gcttcaggca ttaatgatgg tgcagcagca gtactgatga 4980 tgagtgcgga caaagcagca gaattaggtc ttaagccatt ggcacgtatt aaaggctatg 5040 ccatgtctgg tattgagcct gaaattatgg ggcttggtcc tgtcgatgca gtaaagaaaa 5100 ccctcaacaa agcaggctgg agcttagatc aggttgattt gattgaagcc aatgaagcat 5160 ttgctgcaca ggctttgggt gttgctaaag aattaggctt agacctggat aaagtcaacg 5220 tcaatggcgg tgcaattgca ttgggtcacc caattggggc ttcaggttgc cgtattttgg 5280 tgactttatt acatgaaatg cagcgccgtg atgccaagaa aggcattgca accctctgtg 5340 ttggcggtgg tatgggtgtt gcacttgcag ttgaacgtga ctaagtacac cattgcatcg 5400 aatcttgaaa cttgataaag attgacaata aattcaatac ataatgggag ctcaggcttc 5460 cattatttct agctgagcgc atttctaata ttaaggcttc tagctcagca ttgattttag 5520 tatttggcga ttttaaggga cgtctactct gactacttaa tccatcaata ccttgctcag 5580 aatatcgttt ccaccacttg cgtaacgttg gtctaga 5617 // |
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