EBI Dbfetch

ID   L37108; SV 1; linear; genomic DNA; STD; PRO; 1953 BP.
XX
AC   L37108;
XX
DT   15-MAR-1995 (Rel. 43, Created)
DT   30-JUN-2006 (Rel. 88, Last updated, Version 4)
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DE   Methanobacterium thermoautotrophicum F420-dependent
DE   methylenetetrahydromethanopterin dehydrogenase (mtd) gene, complete cds.
XX
KW   F420-dependent methylenetetrahydromethanopterin dehydrogenase.
XX
OS   Methanothermobacter thermautotrophicus
OC   Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales;
OC   Methanobacteriaceae; Methanothermobacter.
XX
RN   [1]
RP   1-1953
RX   DOI; 10.1074/jbc.270.6.2827
RX   PUBMED; 7852356.
RA   Mukhopadhyay B., Purwantini E., Pihl T.D., Reeve J.N., Daniels L.;
RT   "Cloning, sequencing, and transcriptional analysis of the coenzyme
RT   F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene
RT   from Methanobacterium thermoautotrophicum strain Marburg and functional
RT   expression in Escherichia coli";
RL   J. Biol. Chem. 270(6):2827-2832(1995).
XX
CC   NCBI gi: 685179
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1953
FT                   /organism="Methanothermobacter thermautotrophicus"
FT                   /strain="Marburg"
FT                   /mol_type="genomic DNA"
FT                   /clone="pPM58 and pBE103 (subclone for the sequence from
FT                   position 245 to 1428)"
FT                   /db_xref="taxon:145262"
FT   CDS             106..552
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /note="ORFX; NCBI gi: 685180"
FT                   /db_xref="GOA:Q50770"
FT                   /db_xref="InterPro:IPR005074"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q50770"
FT                   /protein_id="AAC41462.1"
FT                   /translation="MNTGIKMEVFDMKSFLGDEVIVRQSSGYSCGPAALATVLRNLGVH
FT                   CSEAELAELAGTDESGTTMYGLILAATSKGVSARGVRMEISDLRRNHIAFVRYGDTAHY
FT                   TVVLSVDDRNITLADPAMGRIRIRRDIFSKIFTGNVLVVEREED"
FT   TATA_signal     684..690
FT                   /gene="mtd"
FT   RBS             711..718
FT                   /gene="mtd"
FT   mRNA            713..>1551
FT                   /note="Location changed from '713..(1551.1953)' to
FT                   '713..>1551'"
FT   5'UTR           713..720
FT   CDS             721..1551
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="mtd"
FT                   /product="F420-dependent methylenetetrahydromethanopterin
FT                   dehydrogenase"
FT                   /note="NCBI gi: 685181"
FT                   /db_xref="GOA:P55300"
FT                   /db_xref="InterPro:IPR002844"
FT                   /db_xref="UniProtKB/Swiss-Prot:P55300"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAC41463.1"
FT                   /translation="MVVKIGIIKCGNIGTSPVLDLLLDERADRPNIDVCVVGSGAKMNP
FT                   DEIERAVPTMLEMERDFVIFISPNPGAPGPAKARELLSAADVPAMIIGDAPGLRVKDEI
FT                   EEQGLGYIIVKADPMIGARREFLDPTEMASFNSDVIKVLAFTGAYRVVQNTIDAMIADV
FT                   EAGKAPELPQVVIDTDKAVEAAGYTNPYAKAKAMAAYEIATKVADIDVRGCFMVQDPDQ
FT                   YIPIVASAHEMLSAAAKLAIEAREIEKANDTVLRTPHGKEGKTLSKKDLLAKPE"
XX
SQ   Sequence 1953 BP; 580 A; 412 C; 474 G; 487 T; 0 other;
     caacctcttt ttcatttgaa gcaagaccgc cgtgagttat ataagctcat gggttctgtt        60
     tcacttcaat catatacatt tattaataat aattaataat ttattatgaa tactggtatt       120
     aaaatggagg tttttgatat gaaatcgttt ctgggtgatg aggttatagt gaggcagagc       180
     agcggataca gctgcggtcc ggcggcactt gccacggttc tcagaaacct tggagttcac       240
     tgcagcgagg cagaactggc ggagcttgca ggtacagacg aatcagggac caccatgtat       300
     gggctgatac tcgcagcaac ctcgaagggg gtgagtgcaa ggggtgtcag gatggaaata       360
     tcggatctga gaaggaatca catcgcattt gtaaggtacg gtgacacggc acactacacc       420
     gttgttcttt cggttgatga tagaaacatc accctggctg accctgccat ggggaggatc       480
     aggataagga gggatatctt ctcaaaaata ttcacaggca acgttcttgt ggttgaaagg       540
     gaggaggatt aataaaccta atattaaatt aaatgattaa gatattatct ataatttaaa       600
     ttttaatcat catgatccca gaaaaggtaa aaaatatact actggtataa aagcttaatc       660
     ataatcttta aatcataaaa tacttaataa ggcgcaaaat aataagattc tggtgatcta       720
     atggttgtaa aaattggtat aataaaatgt ggtaacatcg ggacctcacc tgtgctggac       780
     ctgttacttg atgagagggc agacaggcca aacatagatg tctgtgtcgt gggttcaggt       840
     gcaaaaatga accccgatga aatcgaaagg gcagtaccaa ccatgcttga aatggagagg       900
     gactttgtta tattcataag cccaaacccc ggtgcacctg gccctgcaaa ggcaagggag       960
     ctcctttcag cggctgatgt tccagccatg atcataggtg acgcaccagg cctcagggtc      1020
     aaggatgaaa tcgaggagca gggacttgga tacataatag taaaggccga cccgatgata      1080
     ggggcaagaa gggagttcct ggacccaaca gagatggcat ccttcaactc cgacgttata      1140
     aaggtccttg cattcacagg cgcatacagg gttgtgcaga acacaatcga tgcaatgatt      1200
     gcagatgttg aagccggaaa ggcacctgaa cttcctcagg tggtaataga cacagataag      1260
     gcggttgagg cagcaggcta caccaaccca tatgcaaagg ccaaggccat ggctgcatat      1320
     gagatagcaa ccaaggtggc tgacatagac gtcaggggct gcttcatggt acaggaccct      1380
     gaccagtaca taccaatcgt tgcctcagca cacgaaatgc tatctgcagc tgccaaactt      1440
     gcaattgaag caagggaaat cgaaaaggcc aacgacaccg tgctgagaac accacacggt      1500
     aaggaaggca aaacacttag caagaaggat cttctggcca agccagaata gatcttcaac      1560
     cacttatttt ttctgatata tgagcctttc aacggggatt taattctcac ctttgcaacg      1620
     ttccagagca tggtttatat tcccggatgc ttaatatcac tggtccagcg gtccatataa      1680
     aaaatagatt gcatggatct tggttgaaat tggctgtcgt ttaccgtcgt cagatgctct      1740
     cctcactgac cctgaagatc aacttcccat ctctgtagac cctcaattct cctcgaagga      1800
     aggcctccca tttttcactg gtcagaggtt tacttgcgat tatgtaaccc tcctgatcag      1860
     gctttttctc atgggcgagg tctatctggt aatcgtcatc aaggagttca acaggaccgt      1920
     agggcgcctt tcgatgcaac tggtggaggc cct                                   1953
//