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EBI DbfetchID L35928; SV 1; linear; genomic DNA; STD; PRO; 4853 BP. XX AC L35928; XX DT 04-MAR-1995 (Rel. 42, Created) DT 18-OCT-2008 (Rel. 97, Last updated, Version 4) XX DE Streptococcus salivarius glucosyltransferase (gtfm) gene, complete cds. XX KW glucosyltransferase. XX OS Streptococcus salivarius OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-4853 RX PUBMED; 7822030. RA Simpson C.L., Giffard P.M., Jacques N.A.; RT "Streptococcus salivarius ATCC 25975 possesses at least two genes coding RT for primer-independent glucosyltransferases"; RL Infect. Immun. 63(2):609-621(1995). XX FH Key Location/Qualifiers FH FT source 1..4853 FT /organism="Streptococcus salivarius" FT /mol_type="genomic DNA" FT /tissue_lib="ATCC 25975" FT /note="primer-independent" FT /db_xref="taxon:1304" FT gene 100..4833 FT /gene="gtfm" FT CDS 100..4833 FT /codon_start=1 FT /transl_table=11 FT /gene="gtfm" FT /product="glucosyltransferase" FT /function="polymerize sucrose into glucan" FT /db_xref="GOA:Q55265" FT /db_xref="HSSP:1H8G" FT /db_xref="InterPro:IPR002479" FT /db_xref="InterPro:IPR003318" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR018337" FT /db_xref="UniProtKB/TrEMBL:Q55265" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC41413.1" FT /translation="MENKVRFKLHKVKKNWVTIGVTTLSMVALAGGSLLAQGKVEADET FT SAPNGDGLQQLSEDGTASLVTTTTVTEQASAQASVSAVATASVSHETSFQAATSAVSQE FT ATAQAQTSPVASQEVAVSSQTQSSGQETQTTEQVSQGQTSTQVAGQTSAQSTPSVTEQA FT RPRVLTNAAPAIATRAADSTIRINANRNTNITITASGTTPNVTIITGPNTPKPNVTVTS FT PNGTRPNVTIVTQPNQPNKPVQPSQPSQPNKPVQPNQPSLDYKPVASNLKTIDGKQYYV FT ENGVVKKNAAIELDGRLYYFDETGAMVDQSKPLYRADAIPNNSIYAVYNQAYDTSSKSF FT EHLDNFLTADSWYRPKQILKDGKNWTASTEKDYRPLLMTWWPDKVTQVNYLNYMSQQGF FT GNKTYTTDMMSYDLAAAAETVQRGIEERIGREGNTTWLRQLMSDFIKTQPGWNSESEDN FT LLVGKDHLQGGALTFLNNSATSHANSDFRLMNRTPTNQTGTRKYHIDRSNGGYELLLAN FT DIDNSNPAVQAEQLNWLHYIMNIGSILGNDPSANFDGVRIDAVDNVDADLLQIASDYFK FT EKYRVADNEANAIAHLSILEAWSYNDHQYNKDTKGAQLSIDNPLRETLLTTFLRKSNYR FT GSLERVITNSLNNRSSEQKHTPRDANYIFVRAHDSEVQAVLANIISKQINPKTDGFTFT FT MDELKQAFEIYNADIAKADKKYTQYNIPAAYATMLTNKDSITRVYYGDLFTDDGQYMAE FT KSPYYNAIDALLRARIKYVAGGQDMKVTKLNGYEIMSSVRYGKGAEEANQLGTAETRNQ FT GMLVLTANRPDMKLGANDRLVVNMGAAHKNQAYRPLLLSKSTGLATYLKDSDVPAGLVR FT YTDNQGNLTFTADDIAGHSTVEVSGYLAVWVPVGASENQDARTKASSTKKGEQVFESSA FT ALDSQVIYEGFSNFQDFVKTPSQYTNRVIAQNAKLFKEWGITSFEFAPQYVSSQDGTFL FT DSIIENGYAFEDRYDIAMSKNNKYGSLKDLMDALRALHAEGISAIADWVPDQIYNLPGK FT EVVTASRTNSYGTPRPNAEIYNSLYAAKTRTFGNDFQGKYGGAFLDELKAKYPAIFERV FT QISNGRKLTTNEKITQWSAKYFNGSNIQGTGARYVLQDNATNQYFSVKAGQTFLPKQMT FT EITGSGFRRVGDDVQYLSIGGYLAKNTFIQVGANQWYYFDKNGNMVTGEQVIDGKKYFF FT LDNGLQLRHVLRQGSDGHVYYYDPKGVQAFNGFYDFAGPRQDVRYFDGNGQMYRGLHDM FT YGTTFYFDEKTGIQAKDKFIRFADGRTRYFIPDTGNLAVNRFAQNPENKAWYYLDSNGY FT AVTGLQTINGKQYYFDNEGRQVKGHFVTINNQRYFLDGDSGEIAPSRFVTENNKWYYVD FT GNGKLVKGAQVINGNHYYFNNDYSQVKGAWANGRYYDGDSGQAVSNQFIQIAANQWAYL FT NQDGHKVTGLQNINNKVYYFGSNGAQVKGKLLTVQGKKCYFDAHTGEQVVNRFVEAARG FT CWYYFNSAGQAVTGQQVINGKQLYFDGSGRQVKGRYVYVGGKRLFCDAKTGELRQRR" FT sig_peptide 100..213 FT /gene="gtfm" FT /note="putative" FT mat_peptide 214..4830 FT /gene="gtfm" FT /product="glucosyltransferase" FT repeat_region 3535..3597 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3625..3687 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3691..3753 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3751..3813 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3817..3879 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3853..3915 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 3889..3915 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4021..4083 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4090..4152 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4150..4212 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4216..4278 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4279..4341 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4354..4416 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4393..4455 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4459..4521 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4519..4581 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4585..4647 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4651..4713 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4711..4773 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 4747..4809 FT /function="glucan binding" FT /rpt_family="YG" FT /rpt_type=OTHER FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 4853 BP; 1465 A; 1104 C; 1091 G; 1193 T; 0 other; cagagattta tgaaaagaag atgatttttt cctatttgta atttgtctga atatatcata 60 gagtagagat gacaacagaa aaaaggatga ttgatataga tggaaaataa ggtacgtttt 120 aaactacaca aggttaagaa aaactgggta actattgggg tgaccactct ctcaatggtt 180 gccttggcag gtggaagcct cctagctcaa ggaaaagtag aggccgatga gacgagcgca 240 cctaacggtg acggcttgca gcaactgagt gaggatggga ctgccagtct agtgacgaca 300 acaactgtta ctgagcaagc tagtgctcaa gcaagtgtgt cagcagtagc aacagccagc 360 gtaagtcacg aaacaagctt ccaggcggcg acaagtgcag tcagccagga ggcaactgct 420 caagcacaaa ctagtccagt tgccagtcaa gaagtggcag tatcttcgca aactcaatcc 480 agtggccaag agacacagac tactgaacag gtgtcacaag gtcagacatc aactcaagta 540 gctgggcaaa caagtgctca gtctactcca agtgtgacag aacaagcaag acctagagtc 600 ttgaccaatg cagcgccagc aattgccaca cgcgctgctg atagcactat tcgtatcaat 660 gccaaccgca atactaacat cacgattacg gccagcggta cgacaccaaa tgtaaccatt 720 atcacagggc caaacacgcc taaaccaaac gtgacggtga caagtccaaa tggcacaaga 780 ccaaatgtga ccattgtaac gcagccaaat caacccaaca aacctgttca accaagtcaa 840 ccgtctcaac ctaacaagcc ggtccaacca aatcagccaa gtcttgacta taaaccagta 900 gcctctaact tgaagactat cgatggcaag cagtactatg ttgaaaatgg cgtcgtgaaa 960 aagaacgcag ccattgagct tgatgggcgt ctttattatt tcgatgagac tggagctatg 1020 gtggatcaaa gtaaaccttt gtatcgtgcc gatgccattc caaataactc tatctatgcg 1080 gtttataacc aggcctatga tacgtcaagt aagagttttg aacacttgga taatttcttg 1140 acggctgata gctggtaccg tccaaaacag attttgaagg atgggaagaa ttggacagct 1200 tcaactgaga aagattatcg tccacttttg atgacttggt ggccagacaa ggtgacacag 1260 gtcaactacc tcaactatat gagccaacaa gggtttggta ataagaccta tacgacagat 1320 atgatgagct atgacttggc agctgctgcc gaaacggttc aacgaggcat cgaggaacgt 1380 attggtcgtg agggcaatac cacttggctt cgccagttga tgtcagactt catcaaaaca 1440 cagcctggct ggaactctga gagtgaggac aatctcttag ttggtaaaga ccacttgcaa 1500 ggaggcgctc tgaccttcct aaataatagt gcgacaagtc atgccaattc agatttccgc 1560 ctcatgaacc gcacaccgac taaccaaacg gggacacgca aatatcatat tgaccgttca 1620 aatggtggtt acgagttgct cttggctaac gatatcgaca actctaaccc agctgttcag 1680 gcagagcaac taaactggct ccactacatc atgaacattg gttctatcct tggcaatgat 1740 ccaagtgcca actttgacgg tgttcgtatc gatgcggtgg acaatgtgga tgcggatctc 1800 ttgcagattg cttctgatta cttcaaggaa aaataccgtg tcgcagataa tgaagcaaat 1860 gccattgctc atttgtcaat ccttgaagct tggtcataca acgaccacca atacaacaag 1920 gataccaagg gtgctcagtt gtctatcgac aatccactac gtgaaacgct tttgacgact 1980 ttcttgcgta agagcaatta ccgtggcagc ttggagcgcg tgattactaa ctctcttaac 2040 aatcgttcaa gtgagcagaa acacacgcca cgtgatgcca attatatttt cgtgcgagcc 2100 catgatagtg aagtgcaagc tgttttggct aatatcatca gcaagcagat taatccaaaa 2160 acagatggtt tcaccttcac tatggatgag ctcaaacagg ccttcgaaat ctacaatgcg 2220 gacatcgcga aggctgataa aaagtacacc cagtacaata tcccagctgc ctatgccaca 2280 atgttgacca acaaggatag tatcactcgt gtttactacg gggacctctt taccgacgat 2340 ggccaataca tggctgaaaa atcaccgtac tataatgcca tcgatgccct gctccgtgct 2400 cgcatcaaat atgtagcagg tggtcaagac atgaaggtta ctaagcttaa tggctatgaa 2460 atcatgtcat ctgtgcgtta tggtaagggg gcagaagaag ccaaccagct tggtactgct 2520 gaaacacgca accaaggaat gctggtcctt acagccaacc gtccagatat gaagttggga 2580 gctaatgatc gtctggtagt caatatggga gctgcccaca aaaatcaggc ttaccgtcca 2640 ttgcttctca gcaaatcgac aggtcttgcg acctacctca aggattctga tgtaccagct 2700 ggattggttc gctatacgga caatcaaggg aacttgacct tcacggcaga tgatattgct 2760 ggtcattcaa ccgttgaagt ttcaggttat ttggcagttt gggtgccagt gggtgcctca 2820 gaaaaccaag atgcccgaac caaggcttcg agcaccaaga agggtgagca ggtctttgaa 2880 tcatcagcag ctcttgattc acaagtcatt tacgaaggct tctcaaactt ccaagatttc 2940 gttaagacac caagtcagta caccaaccgt gttattgctc aaaatgccaa actcttcaaa 3000 gagtggggaa tcacttcctt tgaatttgcg ccacagtatg tgtctagcca agacggcact 3060 ttcttggatt ctatcattga aaatggctac gccttcgagg accgctacga tattgccatg 3120 agcaagaaca acaaatacgg ttcactcaaa gacctcatgg atgcccttcg tgcccttcac 3180 gcagaaggta tctcagccat tgctgactgg gttccagatc aaatttacaa tctccctgga 3240 aaagaagtgg taacagcttc tcgtaccaat agctacggta caccacgtcc aaatgctgaa 3300 atctacaata gcctctacgc agctaaaaca cgtacctttg gaaatgactt ccaaggcaag 3360 tacggtggtg ccttccttga tgaattgaag gcaaaatacc cagcaatctt tgagcgcgtg 3420 cagatttcaa acggccgtaa attgactacc aatgagaaaa tcacgcaatg gtcagccaag 3480 tatttcaatg gaagcaatat ccaaggtact ggagctcgct atgtcctaca agataacgct 3540 accaaccaat acttcagcgt caaagcaggt caaaccttcc ttcctaaaca aatgactgaa 3600 attactggaa gtggtttccg tagggttgga gatgatgtcc aatacctctc aattggtggc 3660 taccttgcta agaatacctt tattcaagtc ggtgccaacc agtggtatta ctttgataag 3720 aatggcaaca tggtcacagg tgagcaggtc attgatggca agaaatactt cttcctagac 3780 aatggtctcc agctacgtca tgtccttcgc caaggtagtg atggtcatgt gtattattac 3840 gatcctaaag gggttcaggc ctttaacgga ttttatgatt ttgcgggtcc tcgccaagac 3900 gttcgctact ttgatggcaa cggtcaaatg tatcgtggcc tccacgacat gtatggcaca 3960 accttctatt ttgatgaaaa gactggtatt caagccaaag acaagttcat ccgttttgcg 4020 gacggacgca cgcgttactt catcccagat acaggaaatc tcgcagtcaa ccgatttgcg 4080 caaaatcctg agaacaaggc ttggtattac ctcgatagca acggttatgc cgtgacagga 4140 ctacaaacca ttaacggtaa gcagtattac tttgacaatg aaggacgtca ggttaaggga 4200 cactttgtca ctatcaataa ccaacgttac ttccttgatg gtgatagtgg tgaaattgct 4260 ccgtcacgct ttgtgacgga aaacaacaag tggtactatg tcgatggcaa tggtaaactg 4320 gttaaaggtg ctcaggtcat caatggtaat cactactatt tcaacaatga ttatagccaa 4380 gtcaagggtg cctgggccaa cggccgttac tatgatggtg actcaggtca ggccgtaagc 4440 aaccaattca ttcaaattgc ggctaaccaa tgggcttacc ttaaccaaga tggtcacaaa 4500 gtaacaggtc ttcaaaatat taacaataaa gtttactatt ttggtagcaa tggtgctcaa 4560 gtcaaaggta aattgctcac tgtccaaggt aagaaatgtt actttgatgc ccacacaggt 4620 gagcaagtgg taaaccgctt tgtcgaagct gcacgtggct gctggtatta ctttaactca 4680 gctggccaag cagtgactgg acaacaggtc atcaatggta aacaacttta cttcgacggt 4740 tcaggtcgtc aagttaaagg acgttatgtt tatgttggtg gtaaacgact cttctgcgat 4800 gccaaaactg gtgaattgag acagcgtcgc taattaatat gtactttaaa aat 4853 // |
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