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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   L34548; SV 1; linear; mRNA; STD; INV; 1206 BP.
XX
AC   L34548;
XX
DT   16-JUL-1994 (Rel. 40, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 8)
XX
DE   Dolichovespula maculata hyaluronidase (Allergen Dol m 2) mRNA, 3' end.
XX
KW   allergen; hyaluronidase; hyaluronoglucosaminidase.
XX
OS   Dolichovespula maculata (bald-faced hornet)
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera;
OC   Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea; Vespidae;
OC   Vespinae; Dolichovespula.
XX
RN   [1]
RP   1-1206
RX   DOI; 10.1074/jbc.270.29.17139
RX   PUBMED; 7876212.
RA   Lu G., Kochoumian L., King T.P.;
RT   "Sequence identity and antigenic cross-reactivity of white face hornet
RT   venom allergen, also a hyaluronidase, with other proteins";
RL   J. Biol. Chem. 270(9):4457-4465(1995).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1206
FT                   /organism="Dolichovespula maculata"
FT                   /mol_type="mRNA"
FT                   /tissue_type="venom"
FT                   /db_xref="taxon:7441"
FT   misc_feature    1..60
FT                   /partial
FT                   /note="The exact function of this region is not clear as no
FT                   Met codon is found and there is a stop codon at position
FT                   19-21. The start of the CDS region was chosen from protein
FT                   sequence data."
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   CDS             <61..1056
FT                   /codon_start=1
FT                   /standard_name="Allergen Dol m 2"
FT                   /product="allergen"
FT                   /function="hyaluronidase and allergen"
FT                   /note="Base 1-246 from 5' RACE clones 9, 19, 32, 39 and
FT                   base 127-1229 from 3'RACE clones 1, 2, 3, 4, 7. Clone 39: G
FT                   for A at position 151. Clone 1: G and C for A and T at
FT                   positions 251 and 259.; Consensus DNA sequence indicates
FT                   that bases at position 151-153 encode an asparagine
FT                   residue, but one clone #9 with a single change at position
FT                   151 encodes an aspartic acid residue."
FT                   /db_xref="GOA:P49371"
FT                   /db_xref="HSSP:1FCQ"
FT                   /db_xref="InterPro:IPR018155"
FT                   /db_xref="UniProtKB/Swiss-Prot:P49371"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAA68279.1"
FT                   /translation="SERPKRVFNIYWNVPTFMCHQYGLYFDEVTNFNIKHNSKDDFQGD
FT                   KISIFYDPGEFPALLPLKEGNYKIRNGGVPQEGNITIHLQRFIENLDKTYPNRNFNGIG
FT                   VIDFERWRPIFRQNWGNMMIHKKFSIDLVRNEHPFWDKKMIELEASKRFEKYARLFMEE
FT                   TLKLAKKTRKQADWGYYGYPYCFNMSPNNLVPDCDATAMLENDKMSWLFNNQNVLLPSV
FT                   YIRHELTPDQRVGLVQGRVKEAVRISNNLKHSPKVLSYWWYVYQDDTNTFLTETDVKKT
FT                   FQEIAINGGDGIIIWGSSSDVNSLSKCKRLREYLLTVLGPITVNVTETVN"
FT   3'UTR           complement(1057..1206)
FT                   /note="Clone 1: G for A at positions 1137 and 1154. Clone
FT                   3: G for A at position 1172. Clone 7: C and A for T at
FT                   positions 1064 and 1184."
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   polyA_site      1206
XX
SQ   Sequence 1206 BP; 390 A; 229 C; 261 G; 326 T; 0 other;
     tatatatatc accaccgatg acatctcccg cctaactttt ccagatcgaa ttgcgaaaaa        60
     tccgagagac cgaaaagagt cttcaacatt tattggaacg ttcctacctt tatgtgtcat       120
     cagtatggcc tatacttcga cgaggttaca aattttaata taaagcataa ttctaaggac       180
     gatttccagg gtgacaagat ctcaattttt tatgatcctg gagaattccc ggcattgttg       240
     ccgctcaaag aaggcaatta taagataaga aacggaggag ttcctcaaga aggtaacata       300
     acgatacatc tccaaagatt tatcgaaaat ttggataaaa catatccaaa taggaacttc       360
     aacggtatcg gtgtgatcga ctttgaaaga tggagaccga tcttccgaca aaattggggc       420
     aatatgatga ttcataagaa gttttcaata gacctagttc gcaatgaaca tccattctgg       480
     gataaaaaga tgatcgaatt ggaggcatct aagaggtttg aaaaatatgc cagacttttc       540
     atggaggaaa ctttgaaatt ggccaaaaag actaggaagc aggccgattg gggctattac       600
     ggatatccct actgttttaa tatgtcgcct aataatctcg tacccgattg tgacgctaca       660
     gcgatgctcg agaacgacaa gatgtcgtgg ctgttcaata atcaaaatgt acttctacca       720
     tccgtctata ttagacacga actgacccct gatcaaagag ttggtttagt ccaaggaaga       780
     gtgaaggaag ctgttaggat atcgaataat ttaaaacatt caccgaaagt gctctcttat       840
     tggtggtacg tgtatcagga cgatacaaac acttttctta ccgagaccga cgtgaaaaag       900
     actttccaag agatagcgat taacggtggg gatggtatca ttatatgggg tagctcgtcc       960
     gacgtaaaca gcttaagtaa atgtaagaga ttacgggagt atctgttgac ggttttggga      1020
     ccaatcacgg ttaacgtgac ggaaaccgtc aactaaagat tatccctaaa cttttagtac      1080
     aatctatgta acctcttgcc gatggcgata ggtgtgttca atgatctgct ttgcgaacgc      1140
     tatcgatgct gcaacgatga atactgcgac aatgccatca cattgaaaag acttttcgca      1200
     ggaagg                                                                 1206
//
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