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EBI DbfetchID L32172; SV 1; linear; genomic DNA; STD; PRO; 2897 BP. XX AC L32172; XX DT 02-MAY-1994 (Rel. 39, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Erwinia carotovora endo-polygalacturonase (peh-1) and pectate lyase (pel-3) DE genes, complete cds. XX KW endo-polygalacturonase; integration host factor; pectate lyase; peh-1 gene; KW pel-3 gene. XX OS Pectobacterium carotovorum OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Pectobacterium. XX RN [1] RP 1-2897 RX PUBMED; 8074530. RA Liu Y., Chatterjee A., Chatterjee A.K.; RT "Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) RT and a closely linked endopolygalacturonase gene (peh-1) of Erwinia RT carotovora subsp. carotovora 71"; RL Appl. Environ. Microbiol. 60(7):2545-2552(1994). XX FH Key Location/Qualifiers FH FT source 1..2897 FT /organism="Pectobacterium carotovorum" FT /sub_species="carotovora" FT /strain="71" FT /mol_type="genomic DNA" FT /db_xref="taxon:554" FT CDS complement(49..1257) FT /codon_start=1 FT /transl_table=11 FT /gene="peh-1" FT /product="endopolygalacturonase" FT /db_xref="GOA:P18192" FT /db_xref="HSSP:1BHE" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P18192" FT /protein_id="AAA57139.1" FT /translation="MEYQSGKRVLSLSLGLIGLFSASAFASDSRTVSEPKTPSSCTVLK FT ADSSTATSTIQKALNNCGQGKAVKLSAGSSSVFLSGPLSLPSGVSLLIDKGVTLRAVNN FT AKSFENAPSSCGVVDTNGKGCDAFITATSTTNSGIYGPGTIDGQGGVKLQDKKVSWWDL FT AADAKVKKLKQNTPRLIQINKSKNFTLYNVSLINSPNFHVVFSDGDGFTAWKTTIKTPS FT TARNTDGIDPMSSKNITIAHSNISTGDDNVAIKAYKGRSETRNISILHNEFGTGHGMSI FT GSETMGVYNVTVDDLVMTGTTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVIDTVY FT EKKEGSNVPDWSDITFKDITSQTKGVVVLNGENAKKPIEVTMKNVKLTSDSTWQINNVN FT VKK" FT RBS complement(1263..1266) FT -10_signal complement(1460..1465) FT -35_signal complement(1484..1489) FT protein_bind 1607..1620 FT /bound_moiety="Integration Host Factor" FT /note="putative" FT -10_signal 1750..1755 FT CDS 1837..2880 FT /codon_start=1 FT /transl_table=11 FT /gene="pel-3" FT /product="pectate lyase" FT /note="putative" FT /db_xref="GOA:Q47465" FT /db_xref="HSSP:1EE6" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/TrEMBL:Q47465" FT /protein_id="AAA57140.1" FT /translation="MFKYLTPIFLCTAAISFQAQADDTMLMLLKKDNATYLSWSTDAGN FT VVRQDVYRSTSSAQAGSEKIAELNSSDRTFTDLTANPQSDYWYWVDTVSGNNSVLKSNA FT ASTAPAPLRAAPLKAASPECKAGAVIKDKTVDCGGITLGLSCSGDSDKQPPVITLENAT FT IKNLRISEKGGSDGIHCKSGNCRIENVIWEDICEDAATNLGKTMTIVGGVAHNTTNGPG FT GKPDKVLQQNAKNSHTIVQGKFTLTGQHGKLWRSCGDCTNNGGPRNLTIISATVNGTID FT SIAGVNRNFGDVAEIRDLRIKGYKEGKPPVCEEFNGVEKGKGKSDKYGEFWDTKNCKVS FT RSNVKPL" XX SQ Sequence 2897 BP; 835 A; 691 C; 592 G; 779 T; 0 other; gggggtaacc gtgcggcgct gtcatgacag aacgatttcg tcactgcgtt atttcttaac 60 gttgacgtta ttgatttgcc atgtgctgtc actcgtcagt ttgacgttct tcatcgtcac 120 ttctatcggc tttttcgcat tctcgccgtt cagcaccacc acacctttgg tttgagacgt 180 aatatcctta aacgtaatgt cgctccagtc aggaacatta cttccctctt ttttctcata 240 taccgtgtca atcacgatcg gtttagccac gttcttcatg actacgttgc tataccgaac 300 accattgaca acgccggctg ctgatttgtc gcttttaata cgtaatccat tcgtggtgcc 360 agtcataacc agatcatcga ccgtcacgtt ataaactccc atcgtttcgc taccgatcga 420 catgccatgt cccgttccaa attcattatg caaaatggaa atattgcgtg tctcagaacg 480 acctttatag gctttgatcg ccacattatc gtcgcccgtc gaaatattac tgtgggcaat 540 ggtgatgttc tttgacgaca tcggatcgat accgtcagtg tttctggcgg tggacggcgt 600 tttgatcgtg gttttccacg cggtgaagcc gtcaccatcg ctgaacacga cgtggaagtt 660 cggagaatta atgagagaga cgttatacag cgtgaaattc ttgctcttat taatctgaat 720 cagccggggg gtattctgtt ttaacttttt cactttggca tcggcagcca gatcccacca 780 gctcaccttt ttatcctgaa gcttcacgcc cccttgtcca tcaatggtgc ccggcccata 840 gattccgcta ttcgtcgtgc ttgtagcggt aataaatgca tcgcagcctt taccgtttgt 900 atccaccacg ccacaggatg acggcgcatt ctcaaaagac ttggcattat tcacagcacg 960 tagggttact cctttgtcga ttaataagct cacgccagaa ggtagagaaa gcgggccact 1020 cagaaaaacg gatgaactcc ctgcgctcag ctttaccgct tttccttgcc cgcaattatt 1080 cagcgctttt tgaattgtac tcgtggcagt actactgtcc gctttaagca ccgtacagga 1140 agacggtgtt ttcggttcac tcaccgttcg ggaatcagat gcaaatgccg atgcgctaaa 1200 caaaccgata agccccagtg ataatgataa aactcgcttg cctgattgat attccatgtc 1260 aaaccttacc ttataagcat ccacaacttt tccatcccca aaaggcatta cgctttcatt 1320 ttgcttaata agaaaaataa aacgccttta tagaaactaa aaattgtcac acatgcctat 1380 gtagattaat gaaaagtcca tttacataaa attattccaa tgcttattcg gaaagcattc 1440 aactttacgc atggttaaaa tatcaccaat aaattaaatg aagtatcaac gaaacgtgga 1500 aacaaaacga atccagtcat gcgcagaagc cagcgggtgg taagcttttc ttctcttcac 1560 agcattcatt catttttctt atttttaata agaaacctca taacatttat caatttgata 1620 aaaaattatt aaataaaaaa taataaataa ataataaaat taaatataaa ataatcacag 1680 actaaattaa ataatctgat taacctcaat aaaatcgacg aattattatt catttcatat 1740 tccctgctat ataatccttc ccgatgcgca aggactccct ctttatttgg aataaataaa 1800 gagaaaaata tccgtttaat ttacaaggaa aaacacatgt ttaagtattt aacaccgata 1860 tttttatgca ccgctgctat ttcttttcag gctcaggctg atgacaccat gctgatgctg 1920 ctgaaaaaag ataatgccac ctatttaagc tggtctaccg atgcaggcaa cgttgttcgc 1980 caggatgtgt atcgcagcac cagtagtgct caggcaggca gcgaaaaaat cgcagagctc 2040 aattccagcg acagaacctt taccgattta accgccaatc cacagtcaga ctattggtat 2100 tgggtggata ccgttagcgg taataatagc gttctgaaat ctaatgctgc ctcaacagca 2160 cctgcgccat tacgtgcagc gccgcttaag gcagccagtc cagaatgtaa agccggtgcg 2220 gtaattaaag ataaaaccgt cgattgtggt ggtattacgc tgggtctgag ctgtagcggt 2280 gacagtgata aacagcctcc tgttatcacg ctggaaaatg ccactattaa gaacctgcgc 2340 atctctgaaa aaggcggttc tgacggtatc cactgtaagt caggcaactg ccgtattgaa 2400 aacgtgatct gggaagacat ctgtgaagat gcagcaacca acctgggtaa aaccatgacc 2460 atcgtcggcg gtgtggcaca taacaccacc aatggtcctg gcggcaaacc ggacaaagtg 2520 ctgcaacaga atgccaaaaa cagccacacg atcgtacagg gcaagttcac cctgaccggt 2580 caacacggca aactgtggcg ctcttgcggt gactgcacca acaacggtgg cccacgtaac 2640 ctgaccatca tcagcgcaac cgttaacggc accatcgaca gcatcgctgg cgtaaaccgt 2700 aactttggcg atgtcgctga aatccgcgat ttgcgtatta aaggctacaa agaaggtaaa 2760 ccgccggtat gtgaagagtt taacggtgta gaaaaaggga aaggaaagtc cgataaatac 2820 ggagagttct gggataccaa gaactgcaaa gtcagccgtt caaacgttaa gccgctgtaa 2880 ttctgccagc agaattc 2897 // |
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