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EBI DbfetchID L27797; SV 1; linear; genomic DNA; STD; PRO; 3912 BP. XX AC L27797; XX DT 11-AUG-1994 (Rel. 40, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus subtilis (smf) gene, 3' end, DNA topisomase gene, complete cds, DE (gid) gene, 5' end. XX KW DNA topoisomerase I; DNA-unwinding protein; smf gene. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3912 RA De Jong S.; RT "Cloning and sequencing of the TopI gene, the gene encoding B.subtilis DNA RT Topoisomase I"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..3912 FT /organism="Bacillus subtilis" FT /strain="8G5" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS <1..485 FT /codon_start=3 FT /transl_table=11 FT /gene="smf" FT /note="putative" FT /db_xref="GOA:P39813" FT /db_xref="InterPro:IPR003488" FT /db_xref="UniProtKB/Swiss-Prot:P39813" FT /protein_id="AAA22762.1" FT /translation="CRKGWVIVSGLASGIDGMSHAASIKAKGRTIGVIAGGFQHIYPRE FT NLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITAY FT QALEQGREVFAVPGSLFDPYPGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF" FT CDS 673..2748 FT /codon_start=1 FT /transl_table=11 FT /product="DNA topoisomerase I" FT /function="DNA unwinding protein: removes negative FT supercoils" FT /db_xref="GOA:P39814" FT /db_xref="HSSP:1ECL" FT /db_xref="InterPro:IPR003602" FT /db_xref="UniProtKB/Swiss-Prot:P39814" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22763.1" FT /translation="MSDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVD FT IEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNS FT DCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLS FT AGRVQSVALRLIIDREKEINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEA FT DVKEILSQLKGNQYTVEKVTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQL FT YEGIDLGREGTVGLITYMRTDSTRISNTAVDEAAAFIDQTYGKEFLGGKRKPAKKNENA FT QDAHEAIRPTSVLRKPSELKAVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNG FT LTFRANGSKVKFSGFMKVYVEGKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPP FT RYTEARLVKTLEERGIGRPSTYAPTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFF FT PEIINVEFTAKMERDLDHVEEGNTEWVKIIDNFYTDFEKRVKKAESEMKEVEIEPEYAG FT EDCELCSSPMVYKMGRYGKFLACSNFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKK FT RVFYGCDRYPDCEFVSWDKPIERKCPKCGKMLVEKKLKKGIQVQCVECDYKEEPQK" FT CDS 2824..>3912 FT /codon_start=1 FT /transl_table=11 FT /gene="gid" FT /note="putative" FT /db_xref="GOA:P39815" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P39815" FT /protein_id="AAA22764.1" FT /translation="MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHT FT DKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAIIAAADECSVPAGGALAVDRHEFAA FT SVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYDAAA FT PIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIF FT FEGCMPIEVMAKRGKKTMLFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQ FT THLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPSLLKPTYQFKNRSDLFFAGQM FT TGVEGYVESAASGLVAGINAAKL" XX SQ Sequence 3912 BP; 1273 A; 743 C; 943 G; 953 T; 0 other; tctgtcgtaa aggttgggtg attgtcagcg gactggcgtc tgggatagac ggaatgtccc 60 atgctgcaag tattaaggcg aaggggcgga caattggcgt cattgcaggc ggatttcaac 120 acatttatcc ccgagaaaac cttcagttag cagatcacat ggctaaacac catatcctgc 180 tgtcagagca cccacctgaa actaaacccc aaaaatggca tttccctatg agaaaccgta 240 ttatcagcgg actaagtgaa ggcgttattg tcgttcaggg caaagaaaaa agcggttcgc 300 tgattactgc ctatcaagca ttggaacaag ggagagaggt atttgccgta cccggttcat 360 tgtttgaccc ttaccccgga ggtcctataa aactgatcca gcagggggct aaagccatat 420 ggtcagcaga ggatattttc gaggaacttc ctgagagaaa cgttcaatat acggaaccct 480 tttgaattat cgtttgacaa acggtattgt aatatttaat aatagtgagg atttaaagtt 540 gcatattgtt ttctatttaa ttaatgacgt tcatgataaa gaatgaaatg ttgaaagcca 600 aataaagaca gctgcaaaaa taaaattgaa atatcttgaa aagagagaga acacctcttg 660 agggggatga aaatgtctga ttatctagtc atcgtggaat cgcccgctaa ggcgaaaacg 720 attgaacgtt acttaggtaa aaaatataaa gtaaaagcat caatgggaca tgtccgggat 780 cttccaaaaa gtcaaatggg agttgacata gaacagaatt ttgaaccgaa atatattacc 840 attcgaggta aaggcccggt attaaaagag ctgaaaacgg ctgcgaaaaa agccaaaaaa 900 gtgtatctcg cagctgaccc cgacagagaa ggggaagcga ttgcatggca tttggcacac 960 agtcttgatt tagatctcaa ctcagactgc cgtgtggtgt ttaacgaaat tacaaaagac 1020 gctattaagg aatcgtttaa gcatccccgc atgatcaata tggatttagt ggatgcacag 1080 caagcgagac gtattttaga caggcttgtc ggatataaaa tcagtccaat cttatggaaa 1140 aaagtcaaaa aagggcttag cgcaggacgc gtgcaatccg ttgccctccg tttgattatt 1200 gaccgtgaaa aagagattaa cgactttaag cctgaggaat attggacaat tgacggtacg 1260 ttcttaaaag gtcaagaaac ctttgaagcg agctttttcg ggaaaaacgg caaaaaactt 1320 cctttaaata gtgaagctga tgtaaaagag attctttctc agctcaaagg gaatcaatat 1380 acagttgaaa aagtaaccaa aaaggaacgc aaacgtaatc ctgctttgcc ttttaccact 1440 tctaccctgc agcaggaagc ggctcgcaag ctcaatttca gagcgaagaa aacgatgatg 1500 attgcacagc aattatatga aggaattgat ttaggaagag aaggaacggt tggtctgatc 1560 acgtatatga gaacggattc aacccgtatt tcaaatacgg ctgttgatga agcagctgca 1620 tttattgatc agacatatgg aaaagagttc ctaggcggaa aacggaagcc tgcgaaaaag 1680 aatgaaaatg cccaggatgc ccacgaagca attcggccga catcagttct tagaaaacca 1740 agtgaattaa aagcagtcct cggcagagac caaatgagac tgtataaatt aatttgggag 1800 cgttttgttg ccagccaaat ggcaccggct gttctcgata caatgagtgt cgacctgaca 1860 aacaacggtt tgacatttcg tgcaaatgga agtaaagtca agttttccgg gtttatgaag 1920 gtgtatgttg aaggaaaaga cgatcaaatg gaagaaaaag accggatgct gcctgactta 1980 caagaaggcg acacggtatt atcaaaagat atagaacctg agcagcattt tacccagccg 2040 cctccgcgat atactgaggc ccggcttgtt aaaacccttg aagaacgcgg tatcggccgt 2100 ccatccacat atgctccgac acttgatact attcagcggc gcggctacgt ggcattggat 2160 aataaacgtt tcgttccgac tgaattaggt cagatcgttc ttgacttgat catggagttt 2220 ttccctgaga tcattaacgt tgagtttaca gctaaaatgg agagagatct tgatcatgtt 2280 gaagaaggaa ataccgaatg ggtcaagatt atcgataatt tctacaccga ttttgaaaaa 2340 cgcgtcaaaa aagccgaatc ggaaatgaag gaagttgaga ttgaaccaga gtatgctgga 2400 gaggattgtg aattgtgcag ttctccaatg gtatataaaa tgggacggta cggtaaattc 2460 ttagcttgct ccaacttccc tgactgccgg aacacgaaac cgattgtgaa acaaatcggt 2520 gtgaagtgcc cgagctgcgg agaaggaaac attgttgagc gaaaatcgaa aaagaaacgg 2580 gtgttttacg gctgtgaccg ttatccggac tgtgaattcg tatcatggga caaaccaatt 2640 gaacgaaaat gcccgaaatg cggaaaaatg ctcgtcgaga aaaaactcaa aaaaggtata 2700 caagtccaat gcgtggaatg cgattataag gaagaaccac agaagtagcg gtgagcaggg 2760 tcttgctcac cttctttgtg tgttaaaaga aggccttgaa taataaacta ggagatgtga 2820 aagatgaacc aacaaacagt gaatgtaatc ggagccggac tcgcaggaag tgaagcagca 2880 tggcagcttg ccaaacgtgg gattcaagtc aaattgtatg aaatgcggcc agttaaacaa 2940 acgcctgcgc atcatacaga taaatttgct gagcttgtct gcagcaactc tcttcgctct 3000 aatacacttg ctaacgctgt cggtgtatta aaggaagaaa tgcgtgcgct tgattcagcc 3060 attatcgctg cggctgacga atgttcggtg cctgccgggg gcgctcttgc agtagaccgt 3120 catgaatttg ccgcaagtgt gacaaatcgg gtgaaaaatc atccaaacgt aaccgttatt 3180 aatgaagaag tgactgaaat tcctgaaggc cctactatca tcgcaacggg tccgttaaca 3240 tctgaatcgc tgtctgccca gctgaaggag ctgactggag aggactattt atatttttat 3300 gacgcagcgg cgccaattgt agaaaaagac agccttgata tggataaagt gtacctgaaa 3360 tcccgttatg ataaaggtga agcagcatat ttgaactgcc cgatgacaga agaagagttt 3420 gaccgttttc atgaagcatt aacatcagca gaaacagtgc cgttaaaaga atttgaaaaa 3480 gagattttct ttgaaggctg catgccgatt gaagtcatgg caaaacgggg taagaaaaca 3540 atgcttttcg gtccgatgaa accagttggt ctagagcatc ctgttacagg aaaacgccct 3600 tatgccgtcg ttcagctcag acaggatgat gctgcgggaa cactttataa tattgtagga 3660 ttccagacac atttaaaatg gggagaccaa aaggaagttc tcaagttgat tccaggactt 3720 gaaaatgtag aaatcgtcag atatggcgtg atgcatagaa acacatttat taactctcca 3780 agcctgttaa agccgactta tcaatttaaa aaccgcagtg atctgttctt tgcaggccaa 3840 atgacgggag tagaaggata tgtggaatca gctgcctcag gacttgtagc aggcattaat 3900 gcggcgaagc tt 3912 // |
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