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EBI DbfetchID L27646; SV 1; linear; genomic DNA; STD; PRO; 5367 BP. XX AC L27646; XX DT 18-JAN-1994 (Rel. 38, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE E. coli phosphogluconate dehydrogenase, GDP-mannose pyrophosphorylase and DE phosphomannomutase (gnd, rfbM1 and rfbK1) genes, complete cds. XX KW GDP-mannose pyrophosphorylase; gnd gene; phosphogluconate dehydrogenase; KW phosphomannomutase; rfbK gene; rfbM gene. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-5367 RX PUBMED; 7515042. RA Jayaratne P., Bronner D., MacLachlan P.R., Dodgson C., Kido N., RA Whitfield C.; RT "Cloning and analysis of duplicated rfbM and rfbK genes involved in the RT formation of GDP-mannose in Escherichia coli O9:K30 and participation of RT rfb genes in the synthesis of the group I K30 capsular polysaccharide"; RL J. Bacteriol. 176(11):3126-3139(1994). XX FH Key Location/Qualifiers FH FT source 1..5367 FT /organism="Escherichia coli" FT /map="44 min." FT /strain="09:K30:H12" FT /isolate="E69" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT CDS 48..1454 FT /codon_start=1 FT /transl_table=11 FT /gene="gnd" FT /product="phosphogluconate dehydrogenase" FT /db_xref="GOA:P37754" FT /db_xref="HSSP:2PGD" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P37754" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21136.1" FT /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIAE FT NPGKKLVPYYTVQEFVESLETPRRILLMVKAGSGTDSAIDSLKPYLDKGDIIIDGGNTF FT FQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIAAVA FT EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLTLSNEELAQTFTEW FT NEGELSSYLYDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLIT FT ESVFPRYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQL FT RAASDEYNWELNYAEIAKIFRAGCIIRAQFLQKITDAYAQNAGIANLLLAPYFKQIADD FT YQQALRDVVAYAVQNGIRVPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGAHTYKRTDK FT EGVFHTEWLE" FT CDS 2472..3887 FT /codon_start=1 FT /transl_table=11 FT /gene="rfbM1" FT /product="GDP-mannose pyrophosphorylase" FT /db_xref="GOA:P37753" FT /db_xref="InterPro:IPR014710" FT /db_xref="UniProtKB/Swiss-Prot:P37753" FT /protein_id="AAA21137.1" FT /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRLS FT GLEVHEPMVICNEEHRFLVAEQLRQLNKLSSNIILEPVGRNTAPAIALAALQATRHGDD FT PLMLVLAADHIINNQPVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTD FT SAHTPYQVARFVEKPDRERAGAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDILEACQ FT AAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSALWE FT VSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMNRERSQD FT VKKAVEFLKQNQRTEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQMHHHR FT AEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEIQSGSYLGE FT DDIIRIKDQYGRC" FT CDS 3911..5281 FT /codon_start=1 FT /transl_table=11 FT /gene="rfbK1" FT /product="phosphomannomutase" FT /db_xref="GOA:P37755" FT /db_xref="HSSP:1K2Y" FT /db_xref="InterPro:IPR005841" FT /db_xref="UniProtKB/Swiss-Prot:P37755" FT /protein_id="AAA21138.1" FT /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVGG FT DVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPMNY FT NGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLMGYV FT DLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGIPNPL FT LPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFLQKQPG FT AKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHHYFRDFA FT YCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYE FT PAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADTALMNAKTEEILALLK" XX SQ Sequence 5367 BP; 1224 A; 1453 C; 1498 G; 1192 T; 0 other; aacaaagcga cgcacgcgtc agtgaccaca ccagacagga gtaagtaatg tccaagcaac 60 agatcggggt tgtcggtatg gctgtgatgg ggcgtaacct tgcgctgaac atcgaaagcc 120 gcggttatac cgtctccgtt ttcaaccgct cccgtgaaaa gaccgaagaa gttattgccg 180 aaaaccctgg caagaagctg gttccttact acacagtaca agagtttgta gaatccctcg 240 aaacaccacg tcgtatcctg ttgatggtga aggccgggtc tggcaccgac agcgccatcg 300 attccctgaa gccttacctc gataaaggcg acatcatcat tgatggcggc aacaccttct 360 tccaagacac catccgtcgt aaccgtgaac tgtctgccga aggttttaac tttatcggta 420 ccggtgtttc cggtggtgaa gagggtgctc tgaaagggcc ttccatcatg cctggtgggc 480 agaaagaagc ctatgagctg gttgcgccga ttctgaagca gattgctgcc gttgctgaag 540 acggtgagcc atgtgtgacc tatatcggtg ctgatggtgc tggccattat gtgaagatgg 600 ttcataacgg catcgagtat ggtgacatgc agttgattgc tgaagcctat gcgctgttga 660 aaggcggtct gacactttcc aacgaagagc tggcgcaaac cttcactgaa tggaacgaag 720 gcgagctaag cagctacctg tacgacatta ccaaagatat cttcaccaag aaggatgaag 780 aaggtaagta cctcgttgat gtcattcttg atgaagcagc aaacaaaggt accggtaagt 840 ggaccagcca gagctcactg gatctcggcg aacctctgtc tctgatcacc gaatccgtat 900 ttcctcgtta catctcttcg cttaaagacc agcgtgtagc tgcttcgaaa gtgctgagtg 960 gtccgcaggc tcaaccggct ggtgataaag ctgaatttat cgaaaaagtt cgtcgcgcgt 1020 tgtacctcgg taagatcgtt tcctatgctc agggcttctc tcagctgcga gctgcttctg 1080 atgaatacaa ctgggagcta aactacgcgg agatcgctaa gattttccgc gcgggctgca 1140 tcattcgtgc ccagttcctg cagaagatta ctgatgctta tgcgcaaaac gctggtattg 1200 ctaatctcct gctggcaccg tacttcaaac agatcgctga tgactatcag caggcgctgc 1260 gtgatgtcgt tgcgtatgcc gtgcagaatg gtattcgggt accgacattc tctgccgcaa 1320 ttgcttacta cgacagctac cgttccgcgg ttctgcccgc taatctgatt caggctcagc 1380 gtgattattt tggtgcgcac acctataagc gtactgataa agaaggtgta ttccataccg 1440 agtggttgga ataatcttta tattccacag tcaggccaat tgacctgact gttaactatt 1500 tttttgttat tctataataa actgatcaaa gtcatcttat ctgcacatct tcgttatttt 1560 tcagttctaa taaaccaaat atttatcttt taaccctaat gactccaact tattgatagt 1620 gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg 1680 acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa 1740 ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca 1800 gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac 1860 gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagac 1920 tgtcatacgc gtaaaacagc cagcgctggc gatttagccc cgacatagcc ccactgttcg 1980 tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc 2040 ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggcag 2100 ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt 2160 gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc 2220 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg 2280 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc 2340 agtaagttgg cagcatcacc tcttttaaga atttgccata tttatacttc tgtatatcta 2400 taatattctg cttctggcct cagaatatat cttggcgttt aataacaggg ttttaattaa 2460 aggaaattaa tatgttactt cctgtaatta tggctggcgg taccggcagt cgtctctggc 2520 cgatgtctcg cgagctttat ccgaaacagt tcctccgtct gttcgggcag aactccatgc 2580 tgcaggaaac catcacccga ctctcgggcc ttgaagtcca tgaaccgatg gtcatctgta 2640 acgaagagca ccgcttcctg gtggccgaac agctgcgcca gctcaacaag ctgtccagca 2700 acattattct tgagccggtc gggcgcaaca ccgccccggc catcgcgctg gcggccctcc 2760 aggccacccg ccatggcgac gatccgctga tgctggtcct cgccgccgac catatcatca 2820 ataaccagcc ggtcttccac gacgccatcc gcgttgccga gcagtatgcc gatgaaggcc 2880 atctggtcac cttcggtatc gtgccgaacg ccccggaaac cggctacggc tacatccagc 2940 gcggcgtggc gctcaccgac agcgcccaca ccccgtacca ggtggcccgc ttcgtggaga 3000 agccggaccg cgagcgcgcc ggggcctacc tcgcctccgg ggagtactac tggaacagcg 3060 gcatgtttat gttccgcgca aaaaaatacc tctctgagct ggccaaattc cgcccggata 3120 tcctcgaagc ctgccaggcc gcggtgaatg ccgccgacaa cggtagcgac ttcatcagca 3180 ttccgcatga cattttctgc gagtgcccgg acgagtccgt ggactacgcg gtgatggaga 3240 aaaccgctga tgcggtggtg gtcggtctcg atgctgactg gagcgacgtt ggctcctggt 3300 ccgcactatg ggaggtcagc ccgaaagacg ggcagggtaa tgtcctcagc ggtgacgcgt 3360 gggtgcacaa cagcgaaaac tgctacatca acagcgacga gaagctggta gcggccatcg 3420 gtgtggagaa cctggtgatt gtcagcacca aggacgccgt gctggtgatg aaccgcgagc 3480 gttcccagga cgtgaagaag gcggtcgagt tcctcaagca gaaccagcgc acagagtaca 3540 agcgccaccg cgagatttac cgtccctggg gccgctgcga cgtggtggtc cagaccccgc 3600 gcttcaacgt caaccgcatc acggtgaaac caggcggtgc cttctcgatg cagatgcacc 3660 accatcgcgc cgagcactgg gttattctcg ccggcaccgg tcaggtgacg gtcaacggca 3720 agcagttcct gttgtccgag aaccagtcca cctttattcc gattggcgcc gagcactgcc 3780 tggaaaaccc tggctgtatt ccgctggaag tgctggagat ccagtcgggg tcgtacctcg 3840 gcgaggacga cattattcgt attaaagacc agtatggtcg ttgctaatta ttttcgggac 3900 aagatgcaga atgacacagt taacttgttt taaggcttat gacatccgtg gtgaactggg 3960 tgaggaactg aacgaggaca tcgcctaccg tatcgggcgc gcctacggcg aatttctgaa 4020 acccgggaag atagtggtgg ggggggatgt gcgcctcaca agcgagtcgt tgaagctggc 4080 gctggcccgc gggttaatgg acgccggtac cgatgtgctg gacatcggac tgagcggcac 4140 ggaagagatt tacttcgcca ccttccacct cggggtggac ggcggcatcg aggtgacggc 4200 gagccacaac ccgatgaact acaacggcat gaagctggtg cgcgagaacg cgaagcccat 4260 cagcggcgac accggcctgc gggatatcca gcgcctggcg gaggagaacc agttcccgcc 4320 ggtggacccg gcacgtcgcg ggaccctgcg tcagatttcg gtgctgaagg agtacgtcga 4380 ccacctgatg ggctacgtgg acctggcgaa cttcacccgt ccgctgaagc tggtggtgaa 4440 ctccggcaac ggggcggcgg ggcacgtgat tgatgaggtg gagaaacgct tcgcggcggc 4500 cggggtgccg gtaaccttta tcaaggtgca tcaccagccg gatggccatt tccctaacgg 4560 tatccctaat ccgctgctgc cggagtgccg ccaggatacc gccgacgcgg tgcgcgagca 4620 tcaggccgac atggggattg cctttgacgg cgacttcgat cgctgcttcc tgttcgatga 4680 cgaggcgtcg tttatcgagg ggtattacat tgtcggcctg ctggctgagg cgttcctgca 4740 gaagcagccg ggagcgaaaa tcattcacga cccgcgcctg acgtggaata cggtagacat 4800 cgtgacccgc aacggcggcc agccggtgat gtcgaagacg gggcatgcgt tcatcaagga 4860 gcggatgcgc caggaagacg ccatctacgg cggggagatg agcgcgcacc attacttccg 4920 cgacttcgcc tactgcgaca gcgggatgat cccgtggctg ctggtggcgg agctgctgtg 4980 cctgaagaac agctcgctga aatcgctggt ggcggaccgc caggcggcgt tcccggcgtc 5040 gggggagatc aaccgcaagc tgggaaatgc ggcggaggcg atagcgcgca tccgggcaca 5100 gtatgagccg gcggctgcac acatcgacac aacggacggt atcagtattg aataccctga 5160 gtggcgcttt aacctgcgca cgtccaacac ggagccggtg gtgcgtctga acgtggagtc 5220 cagagcggat actgcgttaa tgaatgcaaa aaccgaagag attttagctc ttctcaagta 5280 atgcattatc cataatggaa gccataggat cagattttaa tcgtattatt gaccttatgg 5340 cttattcttg acatccactg gtcaaag 5367 // |
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