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EBI DbfetchID L22346; SV 1; linear; unassigned DNA; STD; PRO; 3139 BP. XX AC L22346; XX DT 21-NOV-1993 (Rel. 37, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Pyrococcus furiosus alpha-amylase (amyA) gene, complete cds. XX KW alpha-amylase; amyA gene. XX OS Pyrococcus furiosus OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Pyrococcus. XX RN [1] RP 1-3139 RX PUBMED; 8226990. RA Laderman K.A., Asada K., Uemori T., Mukai H., Taguchi Y., Kato I., RA Anfinsen C.B.; RT "Alpha-amylase from the hyperthermophilic archaebacterium Pyrococcus RT furiosus. Cloning and sequencing of the gene and expression in Escherichia RT coli"; RL J. Biol. Chem. 268(32):24402-24407(1993). XX RN [2] RP 1-3139 RA Laderman K.A.; RT ; RL Submitted (27-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL K.A. Laderman, Department of Biology, Johns Hopkins University, Baltimore, RL MD 21218, USA XX DR RFAM; RF01131; sR47. XX FH Key Location/Qualifiers FH FT source 1..3139 FT /organism="Pyrococcus furiosus" FT /mol_type="unassigned DNA" FT /db_xref="taxon:2261" FT gene 716..2665 FT /gene="amyA" FT CDS 716..2665 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:716..718,aa:Met) FT /gene="amyA" FT /product="alpha-amylase" FT /EC_number="3.2.1.1" FT /note="GTG start codon" FT /db_xref="GOA:P49067" FT /db_xref="HSSP:1K1W" FT /db_xref="InterPro:IPR004300" FT /db_xref="UniProtKB/Swiss-Prot:P49067" FT /protein_id="AAA72035.1" FT /translation="MGDKINFIFGIHNHQPLGNFGWVFEEAYEKCYWPFLETLEEYPNM FT KVAIHTSGPLIEWLQDNRPEYIDLLRSLVKRGQVEIVVAGFYEPVLASIPKEDRIEQIR FT LMKEWAKSIGFDARGVWLTERVWQPELVKTLKESGIDYVIVDDYHFMSAGLSKEELYWP FT YYTEDGGEVIAVFPIDEKLRYLIPFRPVDKVLEYLHSLIDGDESKVAVFHDDGEKFGIW FT PGTYEWVYEKGWLREFFDRISSDEKINLMLYTEYLEKYKPRGLVYLPIASYFEMSEWSL FT PAKQARLFVEFVNELKVKGIFEKYRVFVRGGIWKNFFYKYPESNYMHKRMLMVSKLVRN FT NPEARKYLLRAQCNDAYWHGLFGGVYLPHLRRAIWNNLIKANSYVSLGKVIRDIDYDGF FT EEVLIENDNFYAVFKPSYGGSLVEFSSKNRLVNYVDVLARRWEHYHGYVESQFDGVASI FT HELEKKIPDEIRKEVAYDKYRRFMLQDHVVPLGTTLEDFMFSRQQEIGEFPRVPYSYEL FT LDGGIRLKREHLGIEVEKTVKLVNDGFEVEYIVNNKTGNPVLFAVELNVAVQSIMESPG FT VLRGKEIVVDDKYAVGKFALKFEDEMEVWKYPVKTLSQSESGWDLIQQGVSYIVPIRLE FT DKIRFKLKFEEASG" XX SQ Sequence 3139 BP; 945 A; 551 C; 727 G; 916 T; 0 other; ctgcagggga gtttgcaaag ctaataactt cagtagctgg tggaggaggc ggaggaagaa 60 aagaactagc tcaaggtaag ataagagaca tagaaaaagc aaaagaggca atagaaaaag 120 ttaagggctc tctatagctt tctactctcc ttcttttgga atcagaaata tttcatattc 180 tgatctccag aatgggagct tgttcatctt tatttttata taatactcgg tgcttttctc 240 tctgtatatt ttctccacta cttcctgggg cagttggaac tgaactataa tttcagcatc 300 ctctgatacc tttgtttcaa attttgaagt acccttgtaa tcctttccat ttacctttat 360 gagataattt gtgccctctg gaaattctat ttcgaagttg aaatctgaga caattttttc 420 cacttttagc gtaaataacc cccaaccgtc tttttcaact attgtaactg tcctgttttc 480 cttatagaat atctcacttg attctttttc attaatggtt gcaggaggca tttttgccgt 540 tctcatggca agcactagaa ggactataaa aattattgct acagctgtta ttttcttgtc 600 catgctaaca ccctgtaatg agatttggat tttcctatat aaaaagcctt agttattttt 660 gagccattaa atatataagg aagtatcact cttagtgatt aatgggtgga cggaagtggg 720 agataaaatt aacttcatat ttggaattca caaccatcag cccctgggca actttggatg 780 ggtgtttgag gaggcttatg aaaagtgtta ctggccgttt ctggagactc tggaggaata 840 tccaaacatg aaggttgcca ttcatacaag tggccccctc attgagtggc tccaagataa 900 tagacccgaa tacatagact tgcttagaag tctagtgaaa agaggacagg tggagatagt 960 cgttgctggg ttctacgagc ctgtgctagc atcaatccca aaggaagata gaatagagca 1020 gataaggtta atgaaagagt gggctaagag tattggattt gatgctaggg gagtttggct 1080 aactgaaaga gtatggcaac cagagctcgt aaagaccctt aaggagagcg gaatagatta 1140 tgtaatagtt gacgattacc acttcatgag tgcgggatta agtaaagagg agctgtactg 1200 gccatattat acggaagatg gtggggaagt tatagctgtt ttcccgatag atgagaagtt 1260 gagatatttg attcccttta gacccgttga taaggtctta gaatacctgc attctctcat 1320 agatggtgat gagagcaaag ttgcagtatt tcatgacgat ggtgagaagt ttggaatctg 1380 gcctggaact tatgagtggg tgtatgaaaa gggatggtta agagaattct ttgatagaat 1440 ttcaagtgat gaaaagataa acttaatgct ttacactgaa tacttagaaa aatataagcc 1500 tagaggtctt gtttatcttc caatagcttc atattttgag atgagcgaat ggtcattgcc 1560 agcaaagcag gcaaggctct ttgtggagtt cgtcaatgag cttaaagtta aaggtatatt 1620 tgaaaagtac agggtatttg ttaggggagg aatttggaag aatttcttct ataaataccc 1680 agagagcaac tacatgcaca agagaatgct aatggtaagt aagttagtga gaaacaatcc 1740 tgaggccagg aagtatctgc tgagagcaca atgtaacgat gcttattggc acggcctctt 1800 cggtggagta tatttacccc atcttaggag ggccatctgg aacaatttaa tcaaggccaa 1860 cagctatgta agccttggaa aggtcataag ggatatcgac tacgatggct ttgaggaagt 1920 tctcatagag aatgacaact tttatgcagt gtttaaaccc tcttacggtg gttccttggt 1980 ggagttttca tcaaagaata gactcgtgaa ttatgtagat gttctggcaa gaaggtggga 2040 acactatcat ggctatgtgg aaagtcaatt tgatggagta gccagcattc atgagctcga 2100 gaaaaagata ccagatgaaa taagaaaaga agttgcttac gacaagtaca gaaggttcat 2160 gcttcaagat cacgtagtcc ccctgggaac aactctggaa gacttcatgt tctcaagaca 2220 acaggagatc ggagagtttc ctagggttcc atactcatat gaactactag atggaggaat 2280 aaggctgaag agggaacact tgggaataga agttgaaaaa acagtgaagt tagtgaatga 2340 tggatttgag gtggagtata tagtgaacaa caagacagga aatcctgtat tgttcgcagt 2400 ggaacttaac gttgcagttc agagcataat ggagagccca ggagttctaa gggggaaaga 2460 aattgtcgtt gatgacaagt atgcagttgg gaagtttgca ctgaagtttg aagacgaaat 2520 ggaagtctgg aagtatccag taaagactct cagtcaaagt gaaagtggct gggatctaat 2580 ccagcagggt gtcagctaca tagttccaat aaggttggag gataaaataa ggtttaagct 2640 aaaatttgag gaagcctcgg gatagggagg ccctcatcac caatcagggc ccgaaagact 2700 ccctcatcgg cccttctatt ttattttaaa cgtcaatggt ttaccaagtt tccaaaactt 2760 acaaaatgaa caaatctctc cacttgcggg cattccacat atcttgcact ctttgaggtc 2820 tttccccttc acttctggct cgaaaagttt tttctttctt aggaatcctc tcacgaagtt 2880 gaactttgtt ccaggccttt tttcctccaa ttcattgaga acttccttca tgtcaagagt 2940 tgtcgcacct cttgcataag gacactcctc tactatgtac tccaatccaa cggcaatggc 3000 ataggcaaca acttccctct cagttaattc gtagagaggt ttgatcttct ttacgaactt 3060 tccttcccct gggagcagag gacctccctt agccaggtac tctgtattcc agtggagtaa 3120 gttgttcatg agaaagctt 3139 // |
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