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EBI DbfetchID L21902; SV 1; linear; genomic DNA; STD; PRO; 7120 BP. XX AC L21902; XX DT 23-JUL-1993 (Rel. 36, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 5) XX DE Clostridium kluyveri 4-hydroxybutyrate coenzyme A transferase (cat1 and DE cat2), succinate semialdehyde dehydrogenase (sucD), and NAD-dependent DE 4-hydroxybutyrate dehydrogenase (4hbD) genes, complete cds, and sigma DE factor (sigL) gene, partial cds. XX KW 4-hydroxybutyrate dehydrogenase; 4hbD; cat1; cat2; coenzyme A; KW coenzyme A transferase; membrane protein; sigL; sigma factor; KW succinate semialdehyde dehydrogenase; sucD; terminator. XX OS Clostridium kluyveri OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 546-7120 RX DOI; 10.1111/j.1432-1033.1993.tb17641.x RX PUBMED; 8444151. RA Sohling B., Gottschalk G.; RT "Purification and characterization of a coenzyme-A-dependent RT succinate-semialdehyde dehydrogenase from Clostridium kluyveri"; RL Eur. J. Biochem. 212(1):121-127(1993). XX RN [2] RP 546-7120 RX PUBMED; 8550525. RA Sohling B., Gottschalk G.; RT "Molecular analysis of the anaerobic succinate degradation pathway in RT Clostridium kluyveri"; RL J. Bacteriol. 178(3):871-880(1996). XX RN [3] RP 546-7120 RA Sohling B.; RT ; RL Submitted (21-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL Brigitte Sohling, Institut fuer Mikrobiologie, Georg-August-Universitat RL Gottigen, D-06099 Halle, Germany XX RN [4] RP 1-3478 RA Sohling B.; RT ; RL Submitted (15-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Brigitte Sohling, Institut fuer Mikrobiologie, Georg-August-Universitat RL Gottigen, D-06099 Halle, Germany XX DR CABRI; DSM 555. XX CC On Mar 15, 1996 this sequence version replaced gi:403382. XX FH Key Location/Qualifiers FH FT source 1..7120 FT /organism="Clostridium kluyveri" FT /strain="DSM 555" FT /mol_type="genomic DNA" FT /db_xref="taxon:1534" FT RBS 88..93 FT /note="orfZ" FT CDS 102..1391 FT /codon_start=1 FT /transl_table=11 FT /gene="cat2" FT /product="4-hydroxybutyrate coenzyme A transferase" FT /note="orfZ; putative" FT /db_xref="GOA:P38942" FT /db_xref="InterPro:IPR003702" FT /db_xref="UniProtKB/Swiss-Prot:P38942" FT /protein_id="AAA92344.1" FT /translation="MEWEEIYKEKLVTAEKAVSKIENHSRVVFAHAVGEPVDLVNALVK FT NKDNYIGLEIVHMVAMGKGVYTKEGMQRHFRHNALFVGGSTRDAVNSGRAVYTPCFFYE FT VPSLFKEKRLPVDVALIQVSEPDKYGYCSFGVSNDYTKPAAESAKLVIAEVNKNMPRTL FT GDSFIHVSDIDYIVEASHPLLELQPPKLGDVEKAIGENCASLIEDGATLQLGIGAIPDA FT VLLFLKNKKNLGIHSEMISDGVMELVKAGVINNKKKTLHPGKIVVTFLMGTKKLYDFVN FT NNPMVETYSVDYVNNPLVIMKNDNMVSINSCVQVDLMGQVCSESIGLKQISGVGGQVDF FT IRGANLSKGGKAIIAIPSTAGKGKVSRITPLLDTGAAVTTSRNEVDYVVTEYGVAHLKG FT KTLRNRARALINIAHPKFRESLMNEFKKRF" FT repeat_region 1404..1442 FT /rpt_type=INVERTED FT RBS 1513..1517 FT /note="orfY; putative" FT CDS 1526..2461 FT /codon_start=1 FT /transl_table=11 FT /note="orfY; putative membrane protein" FT /db_xref="GOA:P38943" FT /db_xref="InterPro:IPR000620" FT /db_xref="UniProtKB/Swiss-Prot:P38943" FT /protein_id="AAA92345.1" FT /translation="MKKGYIFILLTAIFYSTQEISGKMLAQKGAMDPFQVMMIVFLIGA FT IILLPMAVKDIKVKKLKLTGNDLGYLALCGILAVSISMSMLQFAVTYTKASTAAVLFCT FT NAVFTIPFAYFILKEKIKGITIVSIIVSLIGVVIIFNPAKVMEGIGGSRDLIGICFALV FT AAVVWSLYTVISKKRIEIYGGYVFNCISFFFGVIALLILLVVTGRPIFSGITLNNILVL FT LYMGIFIKAVGYICYLGAIKETSAVTASTVFLIKPALATVLAILILGESIEVNVVIGIV FT FIIIGSIINYSSNKKANDLKKVANTSSAES" FT RBS 2504..2507 FT /gene="cat1" FT /note="putative" FT CDS 2515..4131 FT /codon_start=1 FT /transl_table=11 FT /gene="cat1" FT /product="4-hydroxybutyrate coenzyme A transferase" FT /note="putative; Cat1" FT /db_xref="GOA:P38946" FT /db_xref="InterPro:IPR003702" FT /db_xref="InterPro:IPR017821" FT /db_xref="UniProtKB/Swiss-Prot:P38946" FT /protein_id="AAA92346.1" FT /translation="MSKGIKNSQLKKKNVKASNVAEKIEEKVEKTDKVVEKAAEVTEKR FT IRNLKLQEKVVTADVAADMIENGMIVAISGFTPSGYPKEVPKALTKKVNALEEEFKVTL FT YTGSSTGADIDGEWAKAGIIERRIPYQTNSDMRKKINDGSIKYADMHLSHMAQYINYSV FT IPKVDIAIIEAVAITEEGDIIPSTGIGNTATFVENADKVIVEINEAQPLELEGMADIYT FT LKNPPRREPIPIVNAGNRIGTTYVTCGSEKICAIVMTNTQDKTRPLTEVSPVSQAISDN FT LIGFLNKEVEEGKLPKNLLPIQSGVGSVANAVLAGLCESNFKNLSCYTEVIQDSMLKLI FT KCGKADVVSGTSISPSPEMLPEFIKDINFFREKIVLRPQEISNNPEIARRIGVISINTA FT LEVDIYGNVNSTHVMGSKMMNGIGGSGDFARNAYLTIFTTESIAKKGDISSIVPMVSHV FT DHTEHDVMVIVTEQGVADLRGLSPREKAVAIIENCVHPDYKDMLMEYFEEACKSSGGNT FT PHNLEKALSWHTKFIKTGSMK" FT RBS 4181..4186 FT /gene="sucD" FT /note="putative" FT CDS 4193..5611 FT /codon_start=1 FT /transl_table=11 FT /gene="sucD" FT /product="CoA-dependent succinate semialdehyde FT dehydrogenase" FT /function="coenzyme A- and NADP-dependent succinate FT semialdehyde dehydrogenase" FT /note="SucD" FT /db_xref="GOA:P38947" FT /db_xref="InterPro:IPR015590" FT /db_xref="InterPro:IPR016161" FT /db_xref="InterPro:IPR016162" FT /db_xref="UniProtKB/Swiss-Prot:P38947" FT /protein_id="AAA92347.1" FT /translation="MSNEVSIKELIEKAKAAQKKLEAYSQEQVDVLVKALGKVVYDNAE FT MFAKEAVEETEMGVYEDKVAKCHLKSGAIWNHIKDKKTVGIIKEEPERALVYVAKPKGV FT VAATTPITNPVVTPMCNAMAAIKGRNTIIVAPHPKAKKVSAHTVELMNAELKKLGAPEN FT IIQIVEAPSREAAKELMESADVVIATGGAGRVKAAYSSGRPAYGVGPGNSQVIVDKGYD FT YNKAAQDIITGRKYDNGIICSSEQSVIAPAEDYDKVIAAFVENGAFYVEDEETVEKFRS FT TLFKDGKINSKIIGKSVQIIADLAGVKVPEGTKVIVLKGKGAGEKDVLCKEKMCPVLVA FT LKYDTFEEAVEIAMANYMYEGAGHTAGIHSDNDENIRYARTVLPISRLVVNQPATTAGG FT TVLPISRLVVNQPATTAGGSFNNGFNPTTTLGCGSWGRNSISENLTYEHLINVSRIGYF FT NKEAKVPSYEEIWG" FT RBS 5635..5640 FT /gene="4hbD" FT /note="putative" FT CDS 5648..6763 FT /codon_start=1 FT /transl_table=11 FT /gene="4hbD" FT /product="NAD-dependent 4-hydroxybutyrate dehydrogenase" FT /note="4HbD" FT /db_xref="GOA:P38945" FT /db_xref="InterPro:IPR001670" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:P38945" FT /protein_id="AAA92348.1" FT /translation="MKLLKLAPDVYKFDTAEEFMKYFKVGKGDFILTNEFLYKPFLEKF FT NDGADAVFQEKYGLGEPSDEMINNIIKDIGDKQYNRIIAVGGGSVIDIAKILSLKYTDD FT SLDLFEGKVPLVKNKELIIVPTTCGTGSEVTNVSVAELKRRHTKKGIASDELYATYAVL FT VPEFIKGLPYKFFVTSSVDALIHATEAYVSPNANPYTDMFSVKAMELILNGYMQMVEKG FT NDYRVEIIEDFVIGSNYAGIAFGNAGVGAVHALSYPIGGNYHVPHGEANYLFFTEIFKT FT YYEKNPNGKIKDVNKLLAGILKCDESEAYDSLSQLLDKLLSRKPLREYGMKEEEIETFA FT DSVIEGQQRLLVNNYEPFSREDIVNTYKKLY" FT terminator 6797..6830 FT /note="putative" FT repeat_region 6964..6992 FT /rpt_type=INVERTED FT RBS 7013..7017 FT /gene="sigL" FT /note="putative" FT CDS 7035..>7120 FT /codon_start=1 FT /transl_table=11 FT /gene="sigL" FT /product="sigma factor" FT /note="sigma-54 homologous sigma factor; SigL" FT /db_xref="GOA:P38944" FT /db_xref="InterPro:IPR000394" FT /db_xref="InterPro:IPR007046" FT /db_xref="InterPro:IPR007634" FT /db_xref="UniProtKB/Swiss-Prot:P38944" FT /protein_id="AAA92349.1" FT /translation="MDFALNLTQEQRLAMTQEMQLSIKLLQMS" XX SQ Sequence 7120 BP; 2641 A; 907 C; 1467 G; 2105 T; 0 other; agcttgaaaa aatattggta aaatccgaag aagattttag tgtggtaaaa agtatattga 60 ataagtattt aacaagacag gatagggagg agataacttt tatggagtgg gaagagatat 120 ataaagagaa actggtaact gcagaaaaag ctgtttcaaa aatagaaaac catagcaggg 180 tagtttttgc acatgcagta ggagaacccg tagatttagt aaatgcacta gttaaaaata 240 aggataatta tataggacta gaaatagttc acatggtagc tatgggcaaa ggtgtatata 300 caaaagaggg tatgcaaaga cattttagac ataatgcttt gtttgtaggc ggatctacta 360 gagatgcagt aaattcagga agagcagttt atacaccttg ttttttctat gaagtgccaa 420 gtttgtttaa agaaaaacgt ttgcctgtag atgtagcact tattcaggta agtgagccag 480 ataaatatgg ctactgcagt tttggagttt ccaatgacta taccaagcca gcagcagaaa 540 gtgctaagct tgtaattgca gaagtgaata aaaacatgcc aagaactctt ggagattctt 600 ttatacatgt atcagatatt gattatatag tggaagcttc acacccattg ttagaattgc 660 agcctcctaa attgggagat gtagaaaaag ccataggaga aaactgtgca tctttaattg 720 aagatggagc tactcttcag cttggaatag gtgctatacc agatgcggta cttttattct 780 taaagaacaa aaagaattta ggaatacatt ctgagatgat atcagatggt gtgatggaac 840 tggtgaaggc aggggttatc aataacaaga aaaagaccct ccatccaggc aaaatagttg 900 taacattttt aatgggaaca aaaaaattat atgattttgt aaacaataat ccaatggtag 960 aaacttattc tgtagattat gtaaataatc cactggtaat tatgaaaaat gacaatatgg 1020 tttcaataaa ttcttgtgtt caagtagact taatgggaca agtatgttct gaaagtatag 1080 gattgaaaca gataagtgga gtgggaggcc aggtagattt tattagagga gctaatctat 1140 caaagggtgg aaaggctatt atagctatac cttccacagc tggaaaagga aaagtttcaa 1200 gaataactcc acttctagat actggtgctg cagttacaac ttctagaaat gaagtagatt 1260 atgtagttac tgaatatggt gttgctcatc ttaagggcaa aactttaaga aatagggcaa 1320 gagctctaat aaatatcgct catccaaaat tcagagaatc attaatgaat gaatttaaaa 1380 agagatttta gaatttattt atgattttta gttacacttt atatgaaggt gtaactaaaa 1440 ataagaattc aattgtagtt aatttaacga gatattgtat taaatttaac aaaatgaggc 1500 ttaatttata ggggaggaat gtaaattgaa aaaagggtat atatttattt tattgacggc 1560 aatattttat agcactcagg agatttcagg aaaaatgtta gctcaaaagg gtgctatgga 1620 tccatttcaa gttatgatga ttgtattttt aataggtgcg ataatactgc ttcctatggc 1680 tgtgaaagat ataaaagtca aaaagcttaa acttactggt aatgatttag ggtatcttgc 1740 tctttgtgga atactggcag tatcaatttc tatgtctatg ctccagtttg cagttactta 1800 tactaaggca tctactgcgg cggtattatt ctgtactaat gcagtattta ccataccttt 1860 tgcatatttc atattaaaag aaaagataaa agggattacc atagtttcta ttattgtttc 1920 attgattggt gtagttataa tatttaatcc tgcaaaggtt atggaaggta ttggaggaag 1980 cagggattta ataggaatat gtttcgcact tgtagcagct gtagtttggt ctttatacac 2040 agtaataagt aaaaagagaa ttgagattta tggaggatat gtttttaatt gcatctcttt 2100 cttcttcggg gtaatagcac ttttaattct cttagtagtt actggcagac caatattcag 2160 tggaattact ttaaataata ttctagtgct tttatacatg ggtattttta taaaagctgt 2220 tggttatata tgttatcttg gtgccataaa agagacttcc gctgtaactg catctacagt 2280 ttttcttata aaacctgcac tagctacagt acttgcaatt ttaattttag gtgaaagtat 2340 agaggtaaat gtagttatag gtattgtgtt tataattata ggttctatta taaattattc 2400 tagtaataaa aaggcaaatg atttaaaaaa agttgctaat actagtagtg cagagagtta 2460 attaatagga agtttgtaaa ttaataatat tttaactatt tgtgaggcga ttaaatgagt 2520 aaagggataa agaattcaca attgaaaaaa aagaatgtaa aggctagtaa tgtggcagaa 2580 aagattgaag agaaagttga aaaaacagat aaggttgttg aaaaggcagc tgaggttact 2640 gaaaaacgaa ttagaaactt gaagcttcag gaaaaagttg taacagcaga tgtggcagct 2700 gatatgatag aaaacggtat gattgttgca attagcggat ttactccttc cgggtatcct 2760 aaagaagtac ctaaagcatt gactaaaaaa gttaatgcct tagaggaaga attcaaggta 2820 acactttata caggttcatc tacaggagcc gatatagacg gagaatgggc aaaagcagga 2880 ataatagaaa gaagaattcc atatcagaca aattctgata tgaggaaaaa aataaatgat 2940 ggttctatta agtatgctga tatgcattta agccatatgg ctcaatatat taattattct 3000 gtaattccta aagtagatat agctataata gaggcagtag ctattacaga agaaggggat 3060 attattcctt caacaggaat tggaaataca gctacttttg tggaaaatgc agataaggta 3120 atagtggaaa ttaatgaggc tcaaccgctt gaattggaag gtatggcaga tatatataca 3180 ttaaaaaacc ctccaagaag agagcccata cctatagtta atgcaggcaa taggataggg 3240 accacatatg tgacctgtgg ttctgaaaaa atatgcgcta tagtgatgac aaatacccag 3300 gataaaacaa gacctcttac agaagtgtct cctgtatctc aggctatatc cgataatctt 3360 ataggatttt taaataaaga ggttgaagag ggaaaattac ctaagaacct gcttcctata 3420 cagtcaggag ttggaagtgt agcaaatgca gttttggccg gactttgtga atcaaatttt 3480 aaaaatttga gttgttatac agaagttata caggattcta tgctgaagct tataaaatgt 3540 ggtaaagcag atgtggtgtc aggcacttcc ataagtcctt caccggagat gttgcctgag 3600 ttcataaagg acataaattt ctttagagaa aagatagtat taagaccaca ggaaataagt 3660 aataatccag agatagcaag aagaatagga gttatatcca taaacactgc tttggaagta 3720 gatatatatg gtaatgtaaa ctccactcat gttatgggaa gcaaaatgat gaatggtata 3780 ggcggttctg gagactttgc cagaaatgca tatttgacta tattcactac agagtctatc 3840 gccaaaaaag gagatatatc atctatagtt cctatggtat cccatgtgga tcatacagaa 3900 catgatgtaa tggtaattgt tacagaacag ggagtagcag atttaagagg tctttctcct 3960 agggaaaagg ccgtggctat aatagaaaat tgtgttcatc ctgattacaa ggatatgctt 4020 atggaatatt ttgaagaggc ttgtaagtca tcaggtggaa atacaccaca taatcttgaa 4080 aaagctcttt cctggcatac aaaatttata aaaactggta gtatgaaata aatatcaaca 4140 agtataatac aaaaatttta aatatataaa acatttattt aggaggaaaa atatgagtaa 4200 tgaagtatct ataaaagaat taattgaaaa ggcaaaggcg gcacaaaaaa aattggaagc 4260 ctatagtcaa gaacaagttg atgtactagt aaaagcacta ggaaaagtgg tttatgataa 4320 tgcagaaatg tttgcaaaag aagcagttga agaaacagaa atgggtgttt atgaagataa 4380 agtagctaaa tgtcatttga aatcaggagc tatttggaat catataaaag acaagaaaac 4440 tgtaggcata ataaaagaag aacctgaaag ggcacttgtt tatgttgcta agccaaaggg 4500 agttgtggca gctactacgc ctataactaa tccagtggta actcctatgt gtaatgcaat 4560 ggctgctata aagggcagaa atacaataat agtagcacca catcctaaag caaagaaagt 4620 ttcagctcat actgtagaac ttatgaatgc tgagcttaaa aaattgggag caccagaaaa 4680 tatcatacag atagtagaag caccatcaag agaagctgct aaggaactta tggaaagtgc 4740 tgatgtagtt attgctacag gcggtgctgg aagagttaaa gctgcttact ccagtggaag 4800 accagcttat ggcgttggac ctggaaattc acaggtaata gttgataagg gatacgatta 4860 taacaaagct gcacaggata taataacagg aagaaaatat gacaatggaa ttatatgttc 4920 ttcagagcaa tcagttatag ctcctgctga agattatgat aaggtaatag cagcttttgt 4980 agaaaatggg gcattctatg tagaagatga ggaaacagta gaaaagttta gatcaacttt 5040 atttaaagat ggaaaaataa acagcaagat tataggtaaa tccgtccaaa ttattgcgga 5100 tcttgcagga gtaaaagtac cagaaggtac taaggttata gtacttaagg gtaaaggtgc 5160 aggagaaaaa gatgtacttt gtaaagaaaa aatgtgtcca gttttagtag cattgaaata 5220 tgatactttt gaagaagcag ttgaaatagc tatggctaat tatatgtatg aaggagctgg 5280 tcatacagca ggcatacatt ctgacaatga cgagaacata agatatgcaa gaactgtatt 5340 acctataagc agattagttg taaatcagcc tgcaactact gctggaggaa ctgtattacc 5400 tataagcaga ttagttgtaa atcagcctgc aactactgct ggaggaagtt tcaataatgg 5460 atttaaccct actactacac taggctgcgg atcatggggc agaaacagta tttcagaaaa 5520 tcttacttac gagcatctta taaatgtttc aagaataggg tatttcaata aagaagcaaa 5580 agttcctagc tatgaggaaa tatggggata agtcctgtta ttaaaaagta tataaggagg 5640 aaaaaatatg aagttattaa aattggcacc tgatgtttat aaatttgata ctgcagagga 5700 gtttatgaaa tactttaagg ttggaaaagg tgactttata cttactaatg aatttttata 5760 taaacctttc cttgagaaat tcaatgatgg tgcagatgct gtatttcagg agaaatatgg 5820 actcggtgaa ccttctgatg aaatgataaa caatataatt aaggatattg gagataaaca 5880 atataataga attattgctg tagggggagg atctgtaata gatatagcca aaatcctcag 5940 tcttaagtat actgatgatt cattggattt gtttgaggga aaagtacctc ttgtaaaaaa 6000 caaagaatta attatagttc caactacatg tggaacaggt tcagaagtta caaatgtatc 6060 agttgcagaa ttaaagagaa gacatactaa aaaaggaatt gcttcagacg aattatatgc 6120 aacttatgca gtacttgtac cagaatttat aaaaggactt ccatataagt tttttgtaac 6180 cagctccgta gatgccttaa tacatgcaac agaagcttat gtatctccaa atgcaaatcc 6240 ttatactgat atgtttagtg taaaagctat ggagttaatt ttaaatggat acatgcaaat 6300 ggtagagaaa ggaaatgatt acagagttga aataattgag gattttgtta taggcagcaa 6360 ttatgcaggt atagcttttg gaaatgcagg agtgggagcg gttcacgcac tctcatatcc 6420 aataggcgga aattatcatg tgcctcatgg agaagcaaat tatctgtttt ttacagaaat 6480 atttaaaact tattatgaga aaaatccaaa tggcaagatt aaagatgtaa ataaactatt 6540 agcaggcata ctaaaatgtg atgaaagtga agcttatgac agtttatcac aacttttaga 6600 taaattattg tcaagaaaac cattaagaga atatggaatg aaagaggaag aaattgaaac 6660 ttttgctgat tcagtaatag aaggacagca gagactgttg gtaaacaatt atgaaccttt 6720 ttcaagagaa gacatagtaa acacatataa aaagttatat taatatgtaa cctacaatca 6780 ttaaatatcc catcttaaga gggcatttcc atattgtgaa atgtcctctt ttttatctaa 6840 ataataccgt tctattacta agaaacacct aatataacat ataaggataa tatttctgac 6900 atagtatttg aaaaagtttt catcaatttt gtgacttcat gatataataa tagtggtaga 6960 aaatgattct tatgcgatga acataagaat cacttgataa taatcttaga tgggaggtgt 7020 gtataaaggt gaaaatggat tttgctctta atttaactca ggaacaaaga ttggccatga 7080 cccaggaaat gcaattgtcc ataaagttgc tccaaatgtc 7120 // |
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