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EBI DbfetchID L21196; SV 1; linear; genomic DNA; STD; PRO; 5753 BP. XX AC L21196; XX DT 23-JUL-1993 (Rel. 36, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 11) XX DE Rhodococcus erythropolis CobL (cobL), S-adenosylmethionine-dependent DE methylase (cobM), and precorrin-6x reductase (cobK) genes, complete cds; DE and unknown genes. XX KW . XX OS Rhodococcus erythropolis OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. XX RN [1] RP 1-5753 RX DOI; 10.1016/0378-1119(94)90610-6 RX PUBMED; 8200543. RA De Mot R., Nagy I., Schoofs G., Vanderleyden J.; RT "Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from RT Rhodococcus sp. NI86/21"; RL Gene 143(1):91-93(1994). XX RN [2] RP 1-5753 RX PUBMED; 7836301. RA Nagy I., Schoofs G., Compernolle F., Proost P., Vanderleyden J., de Mot R.; RT "Degradation of the thiocarbamate herbicide EPTC (S-ethyl RT dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 RT involve an inducible cytochrome P-450 system and aldehyde dehydrogenase"; RL J. Bacteriol. 177(3):676-687(1995). XX FH Key Location/Qualifiers FH FT source 1..5753 FT /organism="Rhodococcus erythropolis" FT /strain="NI86/21" FT /mol_type="genomic DNA" FT /db_xref="taxon:1833" FT CDS 380..712 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="putative Orf1" FT /db_xref="UniProtKB/TrEMBL:Q53136" FT /protein_id="AAC37128.1" FT /translation="MNPQRSGDTGRPGRAQVWTRVAENYAAWLLLMAGIASAGVFLTAL FT AGGFEGWSLVAGIVTLLLFAGCWLSFRDSSRREAAEPNMVRYHPDSPDVVIDVDQDTGE FT RQRHRD" FT gene 1057..2400 FT /gene="cobL" FT CDS 1057..2400 FT /codon_start=1 FT /transl_table=11 FT /gene="cobL" FT /product="CobL" FT /function="methylation/decarboxylation of precorrin-6y" FT /note="similar to Cobl of Pseudomonas denitrificans, FT Swiss-Prot Accession Number P21921 and with CbiE FT (methylase) and CbiT(decarboxylase) of Salmonella FT typhimurium, Swiss-Prot Accession Numbers Q05629 and FT Q05632, respectively; putative" FT /db_xref="GOA:Q53137" FT /db_xref="InterPro:IPR013216" FT /db_xref="UniProtKB/TrEMBL:Q53137" FT /protein_id="AAC37129.1" FT /translation="MDSSEWDARYSSVEHPWGSAPAAALSARFAELMPGRAIDLGCGDG FT RHARWLADAGWTVHAVDFSSVAIELARSGGEDRSIDYTVADARTWEPSEPVDLVAIGFL FT HLPVDELVAVIAGAASWLAPSGRLLYLGHALENFTHGVGTLACARLGWAAHDTPVVSLV FT NRPVDTLAPALGDRTRLLVLSRDEHTPAAVAELLTERGFGPSRLTVLGELGGPDESRLD FT GTASAWSHEPGPRLNVIAIDCRAESGALRLTRLPGLPDAAFTGDGQMTKHEVRALTLCA FT LAPAAGEVLWDVGGGSGTIAIEWMRTHPLCRAVTFERSAARVDQITSNAAALGVPSLAV FT FGDAPVAFGDVPADLSAPDAIFVGGGVTQDGLLDECWSRLRVGGRMVANAVTAESEALL FT LQWFSRCGGQLRKFQVYRGEPLGGFTAWRPQLPVAQWSVTKQSKGVVA" FT gene 2397..3146 FT /gene="cobM" FT CDS 2397..3146 FT /codon_start=1 FT /transl_table=11 FT /gene="cobM" FT /standard_name="CobM" FT /product="S-adenosylmethionine-dependent methylase" FT /function="methylation of precorrin-3" FT /note="homology with CobM from Pseudomonas denitrificans FT Swiss-Prot Accession Number P21922 and CbiF from Salmonella FT typhimurium, Swiss-Prot Accession Number P21639; putative" FT /db_xref="GOA:Q53138" FT /db_xref="HSSP:1CBF" FT /db_xref="InterPro:IPR003043" FT /db_xref="UniProtKB/Swiss-Prot:Q53138" FT /protein_id="AAC37130.1" FT /translation="MTVYFIGAGPGAADLITVRAQRIIAASPVCLYAGSLVPQELLAEC FT PEGARVIDTARLSLDEIVALLIEADAAGQDVARLHSGDPSIFSAVAEQVRRLESAGVAY FT QVVPGVPAFTAAAASLGRELTVPGVSQSIVLTRVSTLSTAMPEGEDLRSLGRSGATMVV FT HLGAHRIDQIAEELIEDYGRDCPAAVVAFASRPDEIVLRGTLATIADQVKAAGVTKTAV FT VIVGRVLAAEGFPDSYLYSATRERTTH" FT gene 3149..3895 FT /gene="cobK" FT CDS 3149..3895 FT /codon_start=1 FT /transl_table=11 FT /gene="cobK" FT /standard_name="CobK; precorrin-6x reductase FT (NADPH-dependent)" FT /product="precorrin-6x reductase" FT /function="reduction of precorrin-6x" FT /note="similar to CobK of Pseudomonas denitrificans FT Swiss-Prot Accession Number P21920; putative; gtg start FT codon" FT /db_xref="GOA:Q53139" FT /db_xref="InterPro:IPR003723" FT /db_xref="UniProtKB/Swiss-Prot:Q53139" FT /protein_id="AAC37131.1" FT /translation="MTRILILGGTGEARALAAALADVPGVEAVSSLAGRVRDPRLPVGD FT VRIGGFGGADGLAECVRAHPVDAIVDATHPFAAQITRNAADAAHRRGIPLVVLRRPEWS FT PRPGEHWHGAADLADAAELLPDLGTRIFLTIGRQGVDAFADLQALWFLIRAIDPPDVAM FT PPHSTLLLARGPFAVADETALMREHRIDVLVTKNSGGGQTDAKLDAARALGIPVLMIRR FT PPLPPATETVDDVAGAIAWVGTLTRR" FT CDS complement(3910..4347) FT /codon_start=1 FT /transl_table=11 FT /standard_name="Orf2" FT /product="unknown" FT /note="homology to NirM (cytochrome c551) from Pseudomonas FT stutzeri, without conservation of heme-binding cysteines.; FT putative" FT /db_xref="InterPro:IPR014710" FT /db_xref="UniProtKB/TrEMBL:Q53140" FT /protein_id="AAC37132.1" FT /translation="MLLRSSVVVLSSAVAAVLSASPAQATPSSGVTAQILFETTVPAVA FT GSSAGTDLTLRRIEIAPGGTTGWHFHDGPVYAFVESGVLTRTLQDCTTAVTTAGQAITE FT DHGSDHVHEGSNLGTEPLVLLAFYTEPEGKPLSEDAPAPAC" FT sig_peptide complement(4273..4347) FT /note="signal peptide of ORF2; putative" FT CDS 4554..5294 FT /codon_start=1 FT /transl_table=11 FT /standard_name="Orf3" FT /product="unknown" FT /note="putative Orf3" FT /db_xref="UniProtKB/TrEMBL:Q53141" FT /protein_id="AAC37133.1" FT /translation="MTIEQLLTTHFPDDPVMDAAFASALLRRTGRDPNASGLVRIYSPA FT RTLSFGRLDAVRPQFPAAAAAARHHGFTPVVRGAGGRAAAYHEQTLVVEIVSPDTEGIV FT GARPRFQRLTPRIVAVLRTLGVDARIGPVPGEYCPGDWTVNGAGRVKLAGTAQRIVAGA FT WLFGFELVVADSDPVRAVLADVNAALELEFDPATAASLTDLNSALGLEQVRAAILAEIG FT AVHELTADPDLLVEAANLRDAHVA" XX SQ Sequence 5753 BP; 847 A; 1855 C; 2028 G; 1023 T; 0 other; ggatccggaa cggctggtcc tggcagtagg ccctccgacg gtgttgttgg ttggtgggct 60 gggcgctttc ggacggacct ttcgggtgtg gcgtgcaggt ggagtatggc cgatctggca 120 aggggcagga tggttgctgt tggcgctgat gctcgtctat ttcaccgcat ccagcggcgt 180 tgtggttggt ggctgaccga gccgacgacc aatgtggatg tgtcttcgtg gtaaccggat 240 actgcgcaat agctcatcgc gtacgaaccg ggttgtcggt cgccgctaca tagctggtcg 300 cggagttggg tgaacccaca gcaagttttc agtgacagtc ggcgtccagg tcgacttcga 360 ccgaaggagg taaacgagaa tgaatccgca gaggtccggt gacaccggac gccctggtcg 420 tgcgcaggtc tggacgcggg tggccgaaaa ctatgcggcg tggttgttgc tgatggcggg 480 gatagcgtcg gctggggttt tcctcaccgc tctcgccggc ggcttcgaag gttggtccct 540 cgtcgcgggt atcgtcaccc ttttgctgtt cgcgggttgc tggctgtcgt tccgtgatag 600 ctctcgacgc gaggctgccg agccgaatat ggtccgctac caccccgatt caccggacgt 660 ggtgatcgat gtcgaccaag acaccggcga acggcaacgt caccgggatt gacagttaca 720 acgacggtgc gtgaacgttt cgtctggtgt cgtactctgc gacgagcgcc ggtatcgact 780 gcactgcagc ggaactcgtg cgcctggccc gtcgacgtcg aggagaacgg cgcaatccga 840 tggtccgcgc gtggccgggt ttcgagggcg gtgatagtgc gcgcctcgag gtcctcctcg 900 gcgtcgggac gtctgttgtt cgggtttggc tcgacacgct acgagcgatg gcgtgcagat 960 cgacgttttc gacattgcgc accattcagg tacccctggg ggtactctgg cggattcgag 1020 tactacccgg tccgtgtctt ggaggaatgt gcgtagatgg attcatctga gtgggatgcg 1080 cgttacagca gcgtcgagca tccgtggggc tcggccccgg cggctgccct gtctgcgcgg 1140 ttcgcggagc tgatgccggg ccgggcgatc gacctgggct gtggagacgg ccgccacgct 1200 cgatggttgg ccgacgcggg ctggacagtg cacgctgtcg atttttcttc tgtggcaatc 1260 gaattggcac gcagcggcgg cgaggatcgg tcgatcgact acaccgtcgc cgacgctcgg 1320 acctgggaac catccgaacc ggtggatctg gtggccatcg gcttcctgca tctaccggtc 1380 gacgagttgg tcgccgtcat tgccggtgca gcgagttggc tcgcaccgag cggacggctg 1440 ttgtatctcg gccatgcgct cgagaacttc actcacggtg tcgggaccct ggcctgcgcc 1500 cgcctgggct gggctgcgca cgacacaccg gtggtcagtc tcgtgaaccg gcccgtggac 1560 acgctcgcgc cggcactcgg cgatcggacg cgattgctgg tcctgagccg cgacgagcac 1620 acccccgctg ccgtggccga actcctgacg gaacgaggat tcggaccgtc gcgcctcacc 1680 gtgctcggcg aactgggcgg acccgacgag tcgaggctcg atggaacggc gtcggcgtgg 1740 tcgcacgagc cggggccccg gctgaacgtg atcgcgatcg actgccgcgc cgagtccggc 1800 gccctccggc tgacccggct gccgggactg ccggatgccg cgttcaccgg tgacgggcag 1860 atgaccaaac acgaggtccg cgccctgacg ctgtgcgccc tcgcacccgc ggcgggggag 1920 gtgctgtggg acgtgggagg cggatcgggc acgatcgcga tcgaatggat gcgcacccac 1980 cctctttgcc gggcagtgac tttcgagcgc agcgcggcac gcgtcgacca gatcacgtcg 2040 aacgccgcag cgctcggggt gccgtccctc gcggtgttcg gcgacgcacc cgtggccttc 2100 ggggacgtgc ccgccgacct gtccgctccg gacgcgatct tcgtcggcgg tggcgtcacc 2160 caggacggtc tgctggacga gtgctggtcc cggctccggg tcggcggccg gatggtggcc 2220 aacgcggtga cggcggaatc ggaagcgctc ctgctgcagt ggttctcacg ctgcggtggg 2280 cagctccgca aatttcaggt gtaccggggc gagccgctcg gtgggttcac cgcgtggcgg 2340 ccgcagctgc cggtggcgca gtggtcggtg acgaagcagt cgaaaggtgt tgtggcatga 2400 cggtgtactt catcggagcc ggtcccggtg cggccgacct gatcacggtt cgcgcgcagc 2460 ggatcatcgc ggctagcccg gtctgtctgt acgcgggaag cctcgtcccg caggaactcc 2520 tggcggaatg ccccgagggg gcgcgggtga tcgacacggc gcgtctgagt ctcgacgaga 2580 tcgtcgccct gctgatcgag gccgacgcgg ccggtcagga cgtcgcgcga ctgcattcgg 2640 gtgacccgtc gatcttcagt gccgtcgccg aacaggtccg ccggctcgaa tccgccgggg 2700 tcgcgtacca ggtggtgccc ggagtgcccg cgttcaccgc ggcggcggcg tcgctggggc 2760 gggaactgac cgttcccggt gtgtcgcagt cgatcgtgct gacccgcgtg tccaccctgt 2820 cgaccgcgat gccggagggt gaggacctgc gttccctcgg ccgcagcggc gccacgatgg 2880 tggtgcacct gggggcgcac cggatcgatc agatcgccga agaactcatc gaggactacg 2940 gccgcgactg tccggccgct gtcgtcgcgt tcgcgtcgcg tcccgacgag atcgtgctgc 3000 gcggcaccct ggccaccatc gccgatcagg tgaaggcggc gggcgtcacg aagaccgccg 3060 tggtgatcgt cggtagagtg cttgctgccg aagggtttcc ggacagctac ctgtattccg 3120 ccactcgcga gcgcaccacc cactgagcgt gacgcggatc ctgatcctcg gcggaaccgg 3180 ggaggcccgc gcgctcgcgg cggcgctcgc ggacgttccg ggtgtcgagg ccgtgtcctc 3240 cctcgccggc cgggtccgag acccgcgcct gcccgtcggt gacgtgcgga tcggcggttt 3300 cgggggagcg gacggcctcg cggagtgcgt gcgcgcgcat cccgtcgacg cgatcgtgga 3360 cgcgacgcac ccgttcgcgg cgcagatcac gcgcaacgcc gccgatgccg cgcaccgccg 3420 cggcatccca ctggtggttc tccgtcgccc cgagtggtcg ccgcgtccgg gtgaacattg 3480 gcacggcgcc gcggacctcg ccgacgccgc cgaactgctg cccgatctgg gaacgagaat 3540 ctttctcacc atcggcaggc agggcgtcga tgcgttcgcg gacctgcagg cactgtggtt 3600 cctgatccgc gcgatcgatc cgcccgacgt agcgatgccg ccgcactcga ccctgctcct 3660 ggcccgcggg ccgttcgcgg tcgccgacga gaccgcactg atgcgtgagc accgcatcga 3720 cgtcctcgtc accaagaaca gcggaggcgg tcagaccgac gcgaaactgg acgcggcacg 3780 cgcgctgggc atcccggtac tgatgatccg ccgtccgccg ttgccgccgg cgacggaaac 3840 ggtcgacgac gtcgcgggag ccatcgcctg ggtcggcacg ctcacgcgac gatgacccgg 3900 ccggtccggt cagcaggccg gtgccggcgc atcctcggac aggggcttgc cctccggttc 3960 ggtgtagaac gcgagcagga ccagcggttc cgtacccaga ttggaaccct cgtggacgtg 4020 atcgctgccg tgatcctcgg tgatcgcctg accggcggtc gtcaccgcgg tcgtgcagtc 4080 ctgcagcgtg cgcgtcagca cacccgactc gacgaaggcg tacaccgggc cgtcgtggaa 4140 gtgccacccc gtcgtgccgc cgggggcgat ctcgatgcgc cgtagcgtca gatccgtgcc 4200 cgccgaactt ccggcgaccg ccggaaccgt cgtctcgaac agaatctgtg cggtcacccc 4260 cgaggacggc gtggcctggg cgggggaagc ggacagcacc gcggcgacgg cactggacaa 4320 gacgaccaca ctcgatctga ggagcatcgt gacgacctct cggcgcgctg accctgcaca 4380 ctgcgcgcag gcgtccaggg ggagtgcaat acggcgtgtc cagggtaggg ccacgaaccg 4440 tgcggccgag gggatatccc cgaaatccga tcgccgtgca gactatgtcg gagactcggc 4500 gacacggtag tggtgacgtc tgaggcattc cggagcggaa cgacgggaag agtatgacga 4560 tcgagcagtt actgaccacg cattttccgg atgatcccgt gatggatgcg gcgtttgcct 4620 cggcgctgct gcggcgcacg gggcgagatc cgaatgcgtc tgggctggtg cggatctaca 4680 gtcccgcgcg gacgttgtcg tttgggcggt tggacgctgt tcggccgcag tttccggccg 4740 cggccgcggc ggcgcggcac cacgggttca cgcccgtcgt ccggggcgca ggcggtcggg 4800 ctgcggcata ccacgagcag accctcgtcg tggagatcgt ctccccggac acggagggca 4860 tcgtcggggc ccggccccgc ttccagcgcc tcaccccgag gatcgtcgcc gtactgagga 4920 cgctcggcgt cgatgctcgc atcggaccgg tgcccggcga gtactgcccc ggcgattgga 4980 cggtgaacgg cgcgggccgg gtcaagttgg ccgggaccgc gcagcgaata gtcgccggcg 5040 cgtggctctt cggcttcgag cttgtggtcg ccgattcgga tcccgtgcgg gcggtgctcg 5100 ccgatgtcaa tgctgccctc gagttggagt tcgaccccgc cacggcggcg tccctcactg 5160 acctgaactc ggctctcggt ctcgaacagg tccgggccgc gatccttgcc gagatcggcg 5220 cagtccacga actgaccgca gaccccgacc tcctcgtgga ggccgcgaac ctgcgcgacg 5280 cacacgtggc gtgatcgacc tcagccatca gggcttcctg gggtgttcac ccctttgcga 5340 gcaatgatgc aggccttggt cgctccacga actggcgaac cggtaatcac gcgtaagggg 5400 ggcctccaat cacacctgat tccacctgct gcgggcacct tcgcgcactc caactgtttg 5460 accgcgtaag gggactcgga ccgagattct gtccgtcact actgccggtt accaaagctt 5520 ggctgatgat gattccccgg tggtgcctct tccccgtcca gtgaatgccc ttcgcacacg 5580 gcgagggcta tctcgggatt gttgccgcga ccaaacctgg tgggtgtcga agcgtgtcca 5640 tctccactcc tacacggagg caggttgagt ggaatagcgt gccgcgatct cctcgattcg 5700 atgtcgctcg gcgatcggca tgttccacgc tgctgcgttg gtatgcagga tcc 5753 // |
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