EBI Dbfetch

ID   L21187; SV 1; linear; unassigned DNA; STD; PRO; 1350 BP.
XX
AC   L21187;
XX
DT   23-JUL-1993 (Rel. 36, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 6)
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DE   Streptococcus pyogenes WF14 hyaluronate synthase (hasA) gene, complete cds.
XX
KW   hyaluronate synthase.
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OS   Streptococcus pyogenes
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus.
XX
RN   [1]
RP   1-1350
RX   PUBMED; 8276791.
RA   Dougherty B.A., van de Rijn I.;
RT   "Molecular characterization of hasA from an operon required for hyaluronic
RT   acid synthesis in group A streptococci";
RL   J. Biol. Chem. 269(1):169-175(1994).
XX
RN   [2]
RP   1-1350
RA   Dougherty B.A.;
RT   ;
RL   Submitted (20-JUL-1993) to the EMBL/GenBank/DDBJ databases.
RL   Brian A. Dougherty, Molecular Biology and Genetics, Johns Hopkins
RL   University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205, USA
XX
CC   On Apr 8, 1994 this sequence version replaced gi:310625.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1350
FT                   /organism="Streptococcus pyogenes"
FT                   /strain="WF14"
FT                   /mol_type="unassigned DNA"
FT                   /db_xref="taxon:1314"
FT   -35_signal      2..7
FT   -10_signal      25..30
FT   promoter        36
FT                   /note="transcription start site"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   RBS             62..73
FT                   /note="proposed GTG initiation codon (79..81) downstream
FT                   from RBS; DNA is complementary to the 3' terminus of
FT                   streptococcal 16S rRNA"
FT   CDS             79..1338
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /transl_except=(pos:79..81,aa:Met)
FT                   /gene="hasA"
FT                   /product="hyaluronate synthase"
FT                   /function="required for hyaluronate biosynthesis"
FT                   /note="GTG start codon"
FT                   /db_xref="GOA:P0C0H0"
FT                   /db_xref="InterPro:IPR001173"
FT                   /db_xref="UniProtKB/Swiss-Prot:P0C0H0"
FT                   /protein_id="AAA17725.1"
FT                   /translation="MPIFKKTLIVLSFIFLISILIYLNMYLFGTSTVGIYGVILITYLV
FT                   IKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESLLETLKSVLAQTYPLSEIYIVDDGS
FT                   SNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSDTYI
FT                   YPNALEELLKSFNDETVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSLTGNI
FT                   LVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQSTARCD
FT                   TDVPFQLKSYLKQQNRWNKSFFRESIISVKKILSNPIVALWTIFEVVMFMMLIVAIGNL
FT                   LFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMVKHPASFLLSPLYGILHLFVLQPLKLY
FT                   SLCTIKNTEWGTRKKVTIFK"
FT   misc_feature    1002
FT                   /gene="hasA"
FT                   /note="site of Tn916 insertion in WF62 (hyaluronate
FT                   synthase mutant)"
XX
SQ   Sequence 1350 BP; 427 A; 217 C; 223 G; 483 T; 0 other;
     gttgacatta ccccttattt atattagaat attgaggtcc tgtctttcaa ggaaattaaa        60
     aaagaaagag gtgtaattgt gcctattttt aaaaaaactt taattgtttt atcctttatt       120
     tttttgatat ctatcttgat ttatctaaat atgtatctat ttggaacatc aactgtagga       180
     atttatggag taatattaat aacctatcta gttatcaaac ttggattatc tttcctttat       240
     gagccattta aaggaaagcc acatgactat aaagttgctg ctgtaattcc ttcttataat       300
     gaagatgccg agtcattatt agaaacactt aaaagtgtgt tagcacagac ctatccgtta       360
     tcagaaattt atattgttga tgatgggagt tcaaacacag atgcaataca attaattgaa       420
     gagtatgtaa atagagaagt ggatatttgt cgaaacgtta tcgttcaccg ttcccttgtc       480
     aataaaggaa aacgccatgc tcaagcgtgg gcatttgaaa gatctgacgc tgacgttttt       540
     ttaaccgtag attcagatac ttatatctat ccaaatgcct tagaagaact cctaaaaagc       600
     ttcaatgatg agacagttta tgctgcaaca ggacatttga atgctagaaa cagacaaact       660
     aatctattaa cgcgacttac agatatccgt tacgataatg cctttggggt ggagcgtgct       720
     gctcaatcat taacaggtaa tattttagtt tgctcaggac cattgagtat ttatcgacgt       780
     gaagtgatta ttcctaactt agagcgctat aaaaatcaaa cattcctagg tttacctgtt       840
     agcattgggg atgatcgatg tttaacaaat tatgctattg atttaggacg cactgtctac       900
     caatcaacag ctagatgtga tactgatgta cctttccaat taaaaagtta tttaaagcaa       960
     caaaatcgat ggaataaatc tttttttaga gaatctatta tttctgttaa aaaaattctt      1020
     tctaatccca tcgttgcctt atggactatt ttcgaagtcg ttatgtttat gatgttgatt      1080
     gtcgcaattg ggaatctttt gtttaatcaa gctattcaat tagaccttat taaacttttt      1140
     gcctttttat ccatcatctt tatcgttgct ttatgtcgta atgttcatta tatggtcaaa      1200
     catcctgcta gttttttgtt atctcctctg tatggaatat tacacttgtt tgtcttacag      1260
     cccctaaaac tttattcttt atgcaccatt aaaaatacgg aatggggaac acgtaaaaag      1320
     gtcactattt ttaaataata tatgcatcga                                       1350
//