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EBI DbfetchID L20094; SV 1; linear; genomic DNA; STD; PRO; 3289 BP. XX AC L20094; XX DT 08-JUL-1993 (Rel. 36, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Thermomonospora fusca beta-1,4-endoglucanase precursor (E1) gene, complete DE cds. XX KW . XX OS Thermobifida fusca OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptosporangineae; Nocardiopsaceae; Thermobifida. XX RN [1] RA Lin E., Wilson D.B.; RT "Identification of a celE binding protein and its potential role in RT induction of the celE gene in Thermomonospora fusca"; RL J. Bacteriol. 1701:3843-3846(1988). XX RN [2] RX PUBMED; 1904434. RA Lao G., Ghangas G.S., Jung E.D., Wilson D.B.; RT "DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora RT fusca"; RL J. Bacteriol. 173(11):3397-3407(1991). XX RN [3] RP 1-3289 RX DOI; 10.1002/bit.260420811 RX PUBMED; 18613149. RA Irwin D.C., Spezio M., Walker L.P., Wilson D.B.; RT "Activity studies of eight purified cellulases: Specificity, synergism, and RT binding domain effects"; RL Biotechnol. Bioeng. 42(8):1002-1013(1993). XX RN [4] RP 1-3289 RX PUBMED; 8215374. RA Jung E.D., Lao G., Irwin D., Barr B.K., Benjamin A., Wilson D.B.; RT "DNA sequences and expression in Streptomyces lividans of an exoglucanase RT gene and an endoglucanase gene from Thermomonospora fusca"; RL Appl. Environ. Microbiol. 59(9):3032-3043(1993). XX RN [5] RP 1-3289 RA Irwin D.; RT ; RL Submitted (26-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL Biochemistry, Cornell University, 460 Biotechnology Bldg., Ithaca, NY RL 14853, USA XX FH Key Location/Qualifiers FH FT source 1..3289 FT /organism="Thermobifida fusca" FT /strain="YX" FT /mol_type="genomic DNA" FT /db_xref="taxon:2021" FT protein_bind 104..117 FT /bound_moiety="regulatory protein" FT /citation=[2] FT /citation=[1] FT RBS 168..172 FT /note="complementary to the 3' end of the 16S RNA of S. FT lividans and E. coli" FT sig_peptide 178..273 FT /gene="E1" FT CDS 178..3102 FT /codon_start=1 FT /transl_table=11 FT /gene="E1" FT /product="beta-1,4-endoglucanase precursor" FT /note="the cellulose binding domain includes residues FT 870-974" FT /db_xref="GOA:Q08166" FT /db_xref="HSSP:1K85" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR001919" FT /db_xref="InterPro:IPR003305" FT /db_xref="InterPro:IPR003961" FT /db_xref="InterPro:IPR004197" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008957" FT /db_xref="InterPro:IPR008965" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR012291" FT /db_xref="InterPro:IPR012341" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR014756" FT /db_xref="InterPro:IPR018221" FT /db_xref="UniProtKB/TrEMBL:Q08166" FT /protein_id="AAC06387.1" FT /translation="MLRRPRSRSPLVALTAATCRVALGGTAVPAQADEVNQIRNGDFSS FT GTAPWWGTENIQLNVTDGMLCVDVPGGTVNPWDVIIGQDDIPLIEGESYAFSFTASSTV FT PVSIRALVQEPVEPWTTQMDERALLGPEAETYEFVFTSNVDWDDAQVAFQIGGSDEPWT FT FCLDDVALLGRAEPPVYEPDTGPRVRVNQVGYLPHGPKKATVVTDATSALTWELADADG FT NVVASGQTKPHGADSSSGLNVHTVDFSSYTTKGSDYTLTVDGETSYPFDIDESVYEELR FT VDALSFYYPQRSGIEILDSIAPGYGRPAGHIGVPPNQGDTDVPCAPGTCDYSLDVSGGW FT YDAGDHGKYVVNGGISVHQIMSIYERSQLADTAQPDKLADSTLRLPETGNGVPDVLDEA FT RWEMEFLLKMQVPEGEPLAGMAHHKIHDEQWTGLPLLPSADPQPRYLQPPSTAATLNLA FT ATAAQCARVFEPFDEDFAAECLAAAETAWDAAKANPNIYAPAFGEGGGPYNDNNVTDEF FT YWAAAELFLTTGKEEYRDAVTSSPLHTDDEEVFRDGAFDWGWTAALARLQLATIPNDLA FT DRDRVRQSVVDAADMYLANVETSPWGLAYKPNNGVFVWGSNSAVLNNMVILAVAFDLTG FT DTKYRDGVLEGMDYIFGRNALNQSYVTGYGDKDSRNQHSRWYAHQLDPRLPNPPKGTLA FT GGPNSDSTTWDPVAQSKLTGCAPQMCYIDHIESWSTNELTINWNAPLSWIASFIADQDD FT AGEPGGEEPGPGDDETPPSKPGNLKASDITATSATLTWDASTDNVGVVGYKVSLVRDGD FT AEEVGTTAQTSYTLTGLSADQEYTVQVVAYDAAGNLSTPATVTFTTEKEDETPTPSASC FT AVTYQTNDWPGGFTASVTLTNTGSTPWDSWELRFTFPSGQTVSHGWSANWQQSGSDVTA FT TSLPWNGSVPPGGGSVNIGFNGTWGGSNTKPEKFTVNGAVCSIG" FT mat_peptide 274..3099 FT /gene="E1" FT /product="beta-1,4-endoglucanase" FT /EC_number="3.2.1.4" FT misc_feature 370..735 FT /gene="E1" FT /note="encodes cellulose binding domain; family IV" FT misc_feature 1006..2427 FT /gene="E1" FT /note="encodes catalytic domain; family 9" FT misc_feature 2782..3099 FT /gene="E1" FT /note="encodes cellulose binding domain; family II" XX SQ Sequence 3289 BP; 621 A; 1237 C; 961 G; 470 T; 0 other; ttttgttgtt acagggagga aagagcagcc ggaaagttct attccgagcg taactattga 60 caccgctgag tgatcctgtt tttatgtcat aggtcacatg aaatgggagc gctcccatgg 120 tgggcctccg ctgtgaggtc atcctcccag ttcgaatccc cctacggaag gacttccgtg 180 cttaggagac ccagatctcg atcccccctt gtcgccctca ccgcggcgac ttgccgagtc 240 gcgctcgggg gtacggcggt tcccgcccag gcagacgaag tcaaccagat tcgcaacggc 300 gacttcagct ccggcaccgc accctggtgg ggaaccgaga acatccaact caacgtcacc 360 gacgggatgc tgtgcgtcga cgtccccggc ggcaccgtca acccgtggga cgtgatcatc 420 ggccaggacg acatccccct catcgaaggt gagtcctacg ccttctcctt cactgcctcc 480 agcaccgtcc ccgtctccat ccgcgccctg gtgcaagagc ccgtggagcc gtggaccacc 540 cagatggacg agcgtgccct gctcggcccc gaggcagaaa cctacgaatt cgtcttcacc 600 tccaacgtcg actgggacga cgcccaagtc gccttccaga tcggcggctc cgacgaaccg 660 tggaccttct gcctcgacga cgtcgccctg ctcggccgcg ccgaaccccc ggtctacgaa 720 cccgacaccg gaccgcgggt ccgcgtcaac caggtcggct acctcccgca cggtcccaag 780 aaggcgaccg tggtcaccga cgccaccagc gcgctcacct gggagcttgc cgacgccgac 840 ggtaacgtgg tcgccagcgg ccagaccaag ccgcacggcg cggactccag ctccgggctc 900 aacgtccaca ccgtcgactt cagctcctac accacgaagg gaagcgacta cacgctcacc 960 gtcgacggtg aaaccagcta ccccttcgac atcgacgaaa gcgtctacga ggaactgcgc 1020 gtcgacgcgc tgtcgttcta ctacccgcag cgcagcggca tcgagatcct cgactccatc 1080 gcccccggct acggacgccc ggccggccac atcggcgtgc cccccaacca gggcgatacc 1140 gacgtgccgt gcgcgcccgg cacctgcgac tactccctgg acgtctccgg cggctggtac 1200 gacgcgggcg accacggcaa atacgtggtc aacggcggta tctcggtgca ccagatcatg 1260 agcatctacg agcgctccca gctcgccgac accgcccagc ccgacaagct ggccgactcc 1320 accctgcgcc tgcccgaaac cggcaacggc gtgcccgacg tgctcgacga agcacgctgg 1380 gagatggagt tcctcctcaa gatgcaggtg cccgaaggcg aaccgctcgc cggcatggcg 1440 caccacaaga tccacgacga acagtggacc gggctgccgc tgctgccctc cgctgacccg 1500 cagccgcgct acctgcagcc gccgtccacc gcggccacgc tgaacctggc cgccaccgcc 1560 gcccagtgcg ctcgcgtgtt cgaacccttc gacgaggatt tcgccgccga gtgcctggct 1620 gccgcggaaa ccgcgtggga cgccgccaag gccaacccga acatctacgc gcctgccttc 1680 ggtgaaggcg gcggcccgta caacgacaac aacgtcaccg acgagttcta ctgggccgcg 1740 gccgaactgt tcctcaccac cggcaaggag gagtaccgcg acgcggtgac ctcgtcgccg 1800 ctgcacaccg acgacgaaga ggtcttccgc gacggcgcct tcgactgggg atggactgct 1860 gcgctggccc gcctccagct ggccacgatc cccaacgacc tcgccgaccg cgaccgggtg 1920 cgccagtccg tggtcgatgc cgccgacatg tacctcgcca acgtcgagac cagcccgtgg 1980 ggcctggcct acaagccgaa caacggcgtg ttcgtctggg gctccaacag cgctgtcctc 2040 aacaacatgg tgatcctggc ggtcgccttc gacctcaccg gtgacaccaa ataccgcgac 2100 ggcgtgctgg aaggcatgga ctacatcttc ggccgcaacg cgctgaacca gtcctacgtc 2160 accggctacg gcgacaagga ctcccgcaac cagcacagcc gctggtacgc ccaccagctc 2220 gacccccggt tgcccaaccc gcccaagggc acgctggccg gtggacccaa ctccgactcc 2280 accacctggg acccggtggc ccagtccaag ctgaccgggt gcgcccccca gatgtgctac 2340 atcgaccaca tcgagtcgtg gtccaccaac gagctgacca tcaactggaa cgcccccctg 2400 tcgtggatcg cgtccttcat cgccgaccag gacgacgccg gcgagcccgg cggagaagag 2460 cccggaccgg gcgacgacga gaccccgccg agcaagcctg ggaacctgaa ggccagcgac 2520 atcaccgcga ccagcgccac cctgacctgg gacgcctcca ccgacaacgt cggagtggtc 2580 ggctacaagg tctccctggt ccgcgacggt gacgctgaag aggtgggcac caccgcgcag 2640 accagctaca cgctcaccgg gctgagcgcg gaccaggagt acaccgtcca ggtggtcgcc 2700 tacgacgcgg caggcaacct ctccacgcca gccaccgtca ccttcaccac cgagaaggag 2760 gacgagactc ccacgcccag cgcctcctgc gcggtgacgt accagaccaa cgactggccg 2820 ggcggcttca ccgcctcggt gacgctgacc aacaccggca gcaccccgtg ggactcctgg 2880 gaactgcgct tcaccttccc gtcgggacag actgtcagcc acggctggag cgccaactgg 2940 cagcagagcg gcagtgacgt gaccgccacc tccttgccgt ggaacggatc agttccgccg 3000 ggcggcggct cagtcaacat cggcttcaac ggaacctggg gcggttcgaa caccaaacct 3060 gagaagttca ccgtcaacgg cgcggtctgc tccatcggct gaacctcgcc ctggattcgg 3120 atgtaagccg ccgtggcccc tgtcatcggc gggggccaca cccggcacca tccccgcaca 3180 ccccctgtgc ggggatggtg ctcccctcat tggtctggta agacgaggaa ggcgccaacg 3240 tgacctccga gagccccgtg cgcgcggccc cccgtgccgg acggatgac 3289 // |
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