EBI Dbfetch

ID   L19657; SV 1; linear; genomic DNA; STD; PRO; 1447 BP.
XX
AC   L19657;
XX
DT   13-MAR-1994 (Rel. 38, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 4)
XX
DE   Klebsiella pneumoniae alginate lyase (alyA) gene, complete cds.
XX
KW   alginate lyase; alyA gene.
XX
OS   Klebsiella pneumoniae
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
XX
RN   [1]
RP   1-1447
RX   DOI; 10.1016/0378-1119(94)90605-X
RX   PUBMED; 8200539.
RA   Baron A.J., Wong T.Y., Hicks S.J., Gacesa P., Willcock D., McPherson M.J.;
RT   "Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene cloning,
RT   sequence analysis and high-level production in Escherichia coli";
RL   Gene 143(1):61-66(1994).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1447
FT                   /organism="Klebsiella pneumoniae"
FT                   /sub_species="aerogenes"
FT                   /isolate="25"
FT                   /mol_type="genomic DNA"
FT                   /tissue_lib="genomic"
FT                   /db_xref="taxon:573"
FT   CDS             <1..218
FT                   /codon_start=3
FT                   /transl_table=11
FT                   /note="ORF"
FT                   /db_xref="GOA:Q9XB25"
FT                   /db_xref="UniProtKB/TrEMBL:Q9XB25"
FT                   /protein_id="AAA25048.1"
FT                   /translation="ASVGARGLVSSIEVLGHNAIGTVIALQTTTHNRYCFAICNLPEAE
FT                   QSGEHAVTIDGVTWRWEGAFSRLQRA"
FT   terminator      232..263
FT                   /note="putative"
FT   -35_signal      270..275
FT                   /gene="alyA"
FT   RBS             338..340
FT                   /gene="alyA"
FT   sig_peptide     349..408
FT                   /gene="alyA"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   CDS             349..1272
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="alyA"
FT                   /product="alginate lyase"
FT                   /function="degrades alginates that contain guluronic acid"
FT                   /EC_number="4.2.2.3"
FT                   /note="guluronate-specific"
FT                   /db_xref="GOA:Q59478"
FT                   /db_xref="InterPro:IPR013320"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q59478"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAA25049.1"
FT                   /translation="MLKSGVMVASLCLFSVPSRAAVPAPGDKFELSGWSLSVPVDSDND
FT                   GKADQIKEKTLAAGYRNSDFFTLSDAGGMVFKAPISGAKTSKNTTYTRSELREMLRKGD
FT                   TSIATQGVSRNNWVLSSAPLSEQKKAGGVDGTLEATLSVDHVTTTGVNWQVGRVIIGQI
FT                   HANNDEPIRLYYRKLPHHQKGSVYFAHEPRKGFGDEQWYEMIGTLQPSHGNQTAAPTEP
FT                   EAGIALGETFSYRIDATGNKLTVTLMREGRPDVVKTVDMSKSGYSEAGQYLYFKAGVYN
FT                   QNKTGKPDDYVQATFYRLKATHGAQR"
FT   mat_peptide     409..1269
FT                   /gene="alyA"
FT                   /product="alginate lyase"
FT                   /note="AA 21-51  of the N-terminal mature peptide was
FT                   experimentally determined."
FT   terminator      1288..1307
FT                   /gene="alyA"
FT                   /note="putative"
XX
SQ   Sequence 1447 BP; 356 A; 367 C; 409 G; 315 T; 0 other;
     aagcttccgt cggcgcgcgc ggcctggttt cgtccataga ggtgctgggt cataacgcta        60
     tcggtacggt tatcgccctt cagacgacca cccataaccg ctactgcttt gccatctgta       120
     acctgcctga agcggagcag tcaggcgagc atgccgtcac cattgatggt gtaacctggc       180
     gctgggaggg ggccttctcc cgcctgcaaa gggcgtaatg tgagtcatac cgggcaggtc       240
     agcagacctg cctgttctgt tttagcgtaa tgacaatcct gacaaacatg gcaacgttga       300
     ggttaacgaa agatgaaaag tgagtcccgt tctgaacagg ccgctgtgat gttaaaaagc       360
     ggcgttatgg ttgcatcact gtgtctgttt tctgtcccgt cccgggccgc tgtgccggcg       420
     ccgggggaca aatttgagtt atccggctgg agtctgagtg ttcctgtgga tagtgataac       480
     gatggtaaag ccgatcagat aaaggaaaaa acgctggcgg caggatatcg caactctgat       540
     tttttcaccc tttctgacgc gggcgggatg gtgtttaagg cgccgatctc gggggcaaaa       600
     acctcgaaaa ataccactta tacacgttct gaactgcgcg agatgctgcg caaaggcgat       660
     accagcatcg cgactcaggg cgttagccgt aataactggg ttttatcatc cgcgccgctg       720
     tctgagcaaa aaaaagccgg cggggttgac ggcacgctgg aagccacgct cagtgtggat       780
     cacgtcacca ccacgggggt gaactggcag gtcgggcggg ttattattgg ccagatccac       840
     gccaataacg atgagccgat ccggctctac taccgtaagc tgcctcatca tcagaaaggc       900
     tctgtttact ttgcccatga accaagaaaa ggatttggtg atgagcagtg gtacgaaatg       960
     ataggcacac tgcaacccag tcatggtaac cagacggcgg cgccgacgga gccggaagcg      1020
     gggatagcac tgggagagac gttcagttat cgcattgatg ctaccggaaa taagctcacg      1080
     gtaacgctga tgcgcgaagg gcggccggat gtggtgaaaa cggtcgatat gtcgaagagc      1140
     ggatacagcg aagcgggaca atatctctat tttaaagccg gtgtttataa ccagaataaa      1200
     accggcaaac ctgacgatta tgtccaggca acgttctacc gccttaaagc gacacacgga      1260
     gcgcagcgat agagaaataa atatcctccg gcatagccga ggtttttcat atgcgcctat      1320
     aaggctctct tgccagccgc gccctaacag gcgcattgcg atctgacatt tgcatctatg      1380
     gattacttac ggcccgtaaa cggctacccg gataggggat cgacaactgc tcacccatct      1440
     tatcctc                                                                1447
//