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EBI DbfetchID L18965; SV 1; linear; genomic DNA; STD; PRO; 6760 BP. XX AC L18965; XX DT 02-OCT-1993 (Rel. 37, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Thermophillic bacterial sp. ORF1, 3' end; complete ORF2; xylose repressor DE protein (xylR) gene, complete cds; xynA gene, complete cds; complete ORF5; DE ORF6, 5' end. XX KW repressor protein; transport protein; xylR gene; xynA gene. XX OS Caldicellulosiruptor sp. Rt8B.4 OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor. XX RN [1] RP 1-6760 RX DOI; 10.1007/s002530050653 RX PUBMED; 8920183. RA Dwivedi P.P., Gibbs M.D., Saul D.J., Bergquist P.L.; RT "Cloning, sequencing and overexpression in Escherichia coli of a xylanase RT gene, xynA from the thermophilic bacterium Rt8B.4 genus RT Caldicellulosiruptor"; RL Appl. Microbiol. Biotechnol. 45(1-2):86-93(1996). XX FH Key Location/Qualifiers FH FT source 1..6760 FT /organism="Caldicellulosiruptor sp. Rt8B.4" FT /strain="RT8.B4" FT /mol_type="genomic DNA" FT /db_xref="taxon:28238" FT CDS <1..423 FT /codon_start=1 FT /transl_table=11 FT /note="putative membrane bound sugar transport protein; FT ORF1; putative" FT /db_xref="GOA:P40979" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/Swiss-Prot:P40979" FT /protein_id="AAB42041.1" FT /translation="DPNVAFYSVVAVICWQYIPFYMIFFIAALSNIPQELYEAAKIDGA FT TQGQYFWRIELPLLTPSIKTACILSLIGSLKYFDLIYVMTEGGPSNATELMATYMYKNA FT FASFKMGYGSTIASAMFLIITTAGIFAYFVTRRKEE" FT CDS 456..1283 FT /codon_start=1 FT /transl_table=11 FT /note="putative membrane bound sugar transport protein; FT ORF2" FT /db_xref="GOA:P40980" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/Swiss-Prot:P40980" FT /protein_id="AAB42042.1" FT /translation="MSFIKETKKWYFIFLAVWTLIADVPFLFMLFTSFKTQSELLSGNT FT WQIPRQPTIGNFSTVLEGNFFTYLKNSVIAVSISVVLILIISSMAAFAFSRFKFALNNL FT LYSLIIAGMAIPIHVTLIPIYVLTNKIKLYDTVFALIGPYVALSLPMSIFILTEFMREI FT PLELEEAAKIDGCSMFRLYSDILLPLSAPALITVGIYNGTYLWNEFVFALVLTSSPTRR FT TLPLGIWDFQARYGSDIPAIMAFLTLSLLPMLIAYIFGQDKIIKGMMAGAVKG" FT gene 1371..2570 FT /gene="xylR" FT CDS 1371..2570 FT /codon_start=1 FT /transl_table=11 FT /gene="xylR" FT /note="putative xylose repressor gene; putative" FT /db_xref="GOA:P40981" FT /db_xref="InterPro:IPR000600" FT /db_xref="UniProtKB/Swiss-Prot:P40981" FT /protein_id="AAB42043.1" FT /translation="MGNHALIKQINKLLILKTILDHRTISRAKISKLVDLNKATVSNLT FT DELIKEGFVIEKGYGHSKGGRRPVLLEVNKNVGLIIGIDLGVNYIHLVLTNFIGEIIWE FT RNMAIKLGEKQEKILESLFIILEEAIKAAPPTQKGILGIGIGVPGIVEKNSGTVLLAPN FT LKWQDVPLKRMVESRFGLPVYIDNEANAGALGEKWFGGWGDVSHLLYVSVGIGIGAGIV FT IGEEVYRGAKGFAGEVGHMTIDFNDDVCSCGNVGCLENFASERALLSLIKTLVESGVED FT EYINRDTVEEMDAGYIIQSALQRSRVAMNAITDIANKLGIGIANLVNIFNPDIVVIGNK FT ASFMGDLFLEELRRIVYKRAFITQYHHVRVEVSKLKDRACVLGCVAMVISDMLAFPDYV FT " FT gene 2599..4653 FT /gene="xynA" FT CDS 2599..4653 FT /codon_start=1 FT /transl_table=11 FT /gene="xynA" FT /EC_number="3.2.1.8" FT /db_xref="GOA:P40944" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P40944" FT /protein_id="AAB42044.1" FT /translation="MMRSLKSRKLVFILAMLFLINAIVSLKFITYSSANQLASKSKYIE FT YNFENKSVSPLAKYPSNVVLKVTNSTCAEGTFSVLVAGRKNANDGVIVDITKFLDFSRE FT YEVSFYVLQTTKKLQRISVTLEILDSNDKNQVIAAEKVLLPNIWTKVSAKVQASNYKKA FT KRINLIVNMPTSKSDSFYIDLFTIKDLENAYVLKQENFENKNTGGFLPEDKNCKITLAK FT DRAYSSAYSLKVQPSQKTKNGKILFPIKGLLQKGGTYDFSLLVYQDSSKPVNFSAGIKL FT NDGKSTKEIVLAKQNVAPKKWTQLFATLDLDTRFSAKDVSFFVKPAAAISYYLDLYSIS FT DENWGQPVPDYNLPSLCEKYKNYFKIGVAVPYRALTNPVDVEVIKRHFNSITPENEMKP FT ESLQPYEGGFSFSIADEYVDFCKKDNISLRGHTLVWHQQTPSWFFTNPETGEKLTNSEK FT DKEILLDRLKKHIQTVVGRYKGKVYAWDVVNEAIDENQPDGYRRSDWYNILGPEYIEKA FT FIWAHEADPKAKLFYNDYSTEDPYKREFIYKLIKNLKAKGVPVHGVGLQCHISLDWPDV FT SEIEETVKLFSRIPGLEIHFTEIDISIAKNMTDDDAYNRYLLVQQAQKLKAIFDVLKKY FT RNVVTSVTFWGLKDDYSWLRGDMPLLSDKDYQPKFAFWSLIDPSVVPKE" FT CDS 4887..5498 FT /codon_start=1 FT /transl_table=11 FT /note="ORF5" FT /db_xref="UniProtKB/Swiss-Prot:P40982" FT /protein_id="AAB42045.1" FT /translation="MIENFKEDRCIVCHLKNRAMDKFFDDFLYESVNDYSLRDRIRKGG FT ICPGHARKLESFGDVLAHAIIYSDLLRSFKHNHHIEILPRKKKTQGQNICVFCEKEQGF FT EDIYTRAFSYFFFTSPQFKSAFAERGFICQRHLNQVLEKTASVSAKRELLSVLESKIDK FT ILNHLERIKEKNDYRNIHINYTADEIRAWHMAVEFVAGTQ" FT CDS 5554..>6760 FT /codon_start=1 FT /transl_table=11 FT /note="ORF6" FT /db_xref="GOA:P40983" FT /db_xref="InterPro:IPR001401" FT /db_xref="UniProtKB/Swiss-Prot:P40983" FT /protein_id="AAB42046.1" FT /translation="MRTIEYDVVQDMIRNFTEQLQEISNEINSESIKKLAGSIKEKIEK FT NAFYLVVLGQFKRGKSTLINYMLGANLLPTGVLPLTSVITKIYYSPEVKVDVIFESGVK FT KEIPVDELDLYCTERGNPKNQKCVDTIEIGYPFDFLNKDVVIVDTPGIGSVYQHNTDVT FT YEFIDKSDAVVFVLSVDPPITEVEKQFLLKIAENVDKIFFVINKSDLTSKNEIEEIVSF FT TTNVIKDITKKGNINIFPLSAKMALEGKISKNEEMIEKSCVEIFEKELKQFLKEEKGKI FT QILSNLKSLDGFLGVCEAFLENDMKLKIMPVKQLEENIEKFNEFLERVNQNKIEIYKLF FT KIEMNDILQSFDDEMEKIKKELVVKITKKINDYYPSVARLKRIEQKEHLNKYLEKAIVE FT EFD" XX SQ Sequence 6760 BP; 2321 A; 988 C; 1408 G; 2043 T; 0 other; gatcccaatg tagcttttta ttcagtggtt gctgttatat gctggcaata tatacctttt 60 tatatgatat ttttcatagc agctctgtca aatatacctc aggaactcta tgaggctgca 120 aagatagatg gtgcaacaca ggggcagtat ttctggagaa tagaacttcc tcttttaaca 180 ccttccataa aaacggcctg cattttatct ctaattggtt cattaaaata ttttgattta 240 atttacgtta tgacagaagg tggtccttca aatgcaacag aattaatggc tacatatatg 300 tacaaaaatg ctttcgcgtc attcaagatg ggatatggaa gcactatagc atctgctatg 360 tttcttataa tcacaactgc cggcatattt gcttattttg taacaagacg caaggaagaa 420 tagcttttac attctgtaag ggggttagat attcaatgag tttcataaag gagactaaaa 480 aatggtattt tatatttctg gcagtatgga cattaattgc agatgttcca tttttgttta 540 tgctgtttac ttcatttaaa acacagtcag aacttttatc tggcaacaca tggcaaatac 600 caagacaacc aactataggg aatttttcaa ccgtgttgga gggtaatttt tttacttatt 660 taaaaaacag tgtaatagcg gtctctatat cggttgttct gattttaata atatcttcaa 720 tggctgcttt tgctttttcc aggtttaagt ttgctcttaa caatctactg tattcgctta 780 taattgcagg catggcaata cctattcatg taactttaat accaatttat gtgttaacaa 840 acaagattaa gctttacgat acggtatttg ctttgattgg tccatatgtt gcactcagtt 900 taccaatgtc gatttttata ttaaccgagt ttatgcgtga aatacctctt gaactcgaag 960 aagctgctaa aatagacggt tgttcaatgt tcaggttata ttctgatatt ttattaccct 1020 tatcagcacc agctttgata actgttggta tttacaatgg aacatatctc tggaatgaat 1080 ttgtgtttgc acttgttttg acaagttcac cgacaagaag aactttaccg cttggcattt 1140 gggactttca ggcaagatac ggctctgaca ttcctgccat aatggcattt ttgacattat 1200 cacttttgcc aatgctaatt gcatacatct ttggtcagga caagattata aagggtatga 1260 tggcaggagc tgttaagggt taaaccagtg gtttgagggt atttcattgt ttgaggattt 1320 tacattataa tatttttcaa accacaatta gaataaagga taaagggaag atgggaaatc 1380 acgcattaat taagcaaatc aacaagctgc tgattttaaa aacgatattg gatcatagaa 1440 caatttcgag agctaaaatc tcgaaacttg tagatttaaa taaagcaact gtatcaaatc 1500 ttactgatga gctcataaaa gagggatttg tgatagaaaa aggatacggg cattcaaaag 1560 gtggaagaag gccggtctta cttgaagtta acaaaaatgt gggactcatc attgggatag 1620 accttggtgt taactatatt catttggttt tgacaaattt tattggtgag attatctggg 1680 aaagaaatat ggctatcaaa cttggtgaaa aacaggaaaa gattctggaa agccttttta 1740 taatacttga agaagcaata aaagctgctc caccaacaca aaagggaata cttggaatag 1800 ggataggagt tccaggtatt gttgaaaaaa actctggaac tgtgctttta gcaccaaatt 1860 tgaaatggca ggatgtacct ttgaaaagga tggttgaaag caggtttggg cttccagtat 1920 atatagacaa tgaagcaaac gcaggtgctt tgggcgaaaa atggtttggt ggatggggag 1980 atgtttccca ccttttatat gtaagtgttg gcataggtat tggtgccggg attgtaattg 2040 gtgaagaggt ttacagaggt gcaaaggggt ttgctggaga agttggacat atgacaattg 2100 attttaacga tgatgtatgc agctgtggta atgtcgggtg cttagagaac tttgcatcag 2160 aaagggctct tttgagtctt ataaagacac ttgtggaaag tggagtagaa gatgaatata 2220 taaacaggga tacggttgaa gagatggatg caggttatat aatccagtct gccttgcaaa 2280 gaagcagagt agctatgaat gctatcacag atattgctaa taagcttggt ataggtattg 2340 ccaatttggt caatatattt aatcctgata tagttgtaat aggcaataaa gcatcattta 2400 tgggtgattt gtttttagaa gagctgagaa gaattgtata taaaagagca tttataaccc 2460 agtaccatca tgtaagagtt gaagtttcaa agctaaaaga cagagcctgt gtactgggat 2520 gtgttgcaat ggtgatttct gatatgcttg cattccctga ttatgtataa gaaaagtttt 2580 aaaaggagtg tttatttgat gatgagaagc ctgaaaagtc ggaagctggt ttttattctt 2640 gccatgctgt ttttaataaa cgccatagta tcattgaaat ttataactta ttcatctgca 2700 aaccaattgg cttccaaaag taaatacatt gaatacaatt ttgaaaacaa aagtgtctct 2760 ccacttgcaa aatatccatc caatgttgta ttaaaggtta caaattctac ttgtgcagag 2820 gggacgtttt cggttctggt agcaggaaga aaaaatgcaa atgacggagt tatagttgat 2880 attacaaagt ttttagactt ctcaagagag tatgaagttt ctttttatgt tttacaaacc 2940 acaaaaaaac ttcaaagaat ttctgtaacc ttagaaattt tagattcaaa tgataaaaat 3000 caggttattg ctgctgaaaa ggtgcttttg ccaaacatat ggacaaaggt gagtgcaaaa 3060 gttcaggctt caaattataa aaaggcaaag aggatcaatt tgattgttaa catgccaaca 3120 agcaaaagcg actcatttta cattgacctg tttacaataa aggacttgga aaatgcttat 3180 gttctcaaac aggagaattt cgaaaacaaa aatactggcg gttttttgcc tgaagataaa 3240 aactgcaaaa taacacttgc aaaggataga gcttacagca gcgcttacag tctcaaagtt 3300 cagccatctc aaaagaccaa gaatgggaag attctctttc caataaaggg cctgctgcaa 3360 aagggaggga catatgattt ttcactgctt gtttaccagg acagttcaaa gcctgttaat 3420 ttttcagcag gcataaagct aaacgatgga aaatcgacaa aagagattgt tttggcaaaa 3480 cagaatgttg cacccaaaaa gtggacgcag ctttttgcaa ctcttgatct tgacaccagg 3540 ttttctgcaa aagatgttag cttttttgta aagccagctg ctgcaatctc ctattatttg 3600 gacctttatt caatctcaga tgaaaattgg gggcagcctg tgcctgatta taatctgcca 3660 tcactttgtg aaaagtacaa aaactatttc aagattggag ttgctgtgcc ctacagggct 3720 ctgacaaatc cagttgatgt ggaagtgata aaaaggcatt tcaacagcat aacaccggag 3780 aacgaaatga aaccagagag ccttcagccc tatgaaggtg gttttagctt tagcattgca 3840 gatgagtatg tagatttttg caaaaaggac aatatctcgc tgcgagggca cacccttgtt 3900 tggcatcagc aaaccccgag ctggttcttt acaaatcctg aaacaggcga aaaacttact 3960 aacagtgaga aggacaaaga aatactattg gataggctaa agaagcatat ccagacagtt 4020 gttggcaggt ataaggggaa agtatatgca tgggacgttg tgaatgaggc gattgatgag 4080 aatcagccgg atgggtatag aagaagtgac tggtacaata tcttgggacc ggagtacatt 4140 gaaaaggcat ttatctgggc gcatgaagca gacccgaaag caaagctttt ctacaatgac 4200 tacagtacag aagacccata caagagagaa tttatataca aactaattaa aaatttaaaa 4260 gcaaaaggtg tgccagttca tggtgttggg cttcagtgcc atatttcact tgactggccg 4320 gatgtgagtg aaattgagga gactgtcaaa ttatttagca ggattccagg acttgaaata 4380 cacttcacag aaattgatat aagtattgct aaaaacatga ccgatgatga tgcatacaat 4440 cgctatcttt tggttcagca ggcacaaaaa ttaaaagcaa tttttgacgt tttgaaaaag 4500 tacagaaatg tagttacaag tgttacattc tggggactga aggatgatta ctcatggcta 4560 cggggagata tgccactttt gtccgacaaa gactaccagc caaagtttgc gttctggagc 4620 ttgattgacc catcagttgt cccaaaagag taataaaact aattattgac taaaatatca 4680 aaacttcata taataaaatt gaaaagaaaa agtgaatata gaggcgatgg agttcgccta 4740 aaaatgccac ggcaatagaa aatggcttgt ggctgataac tcctgtccgt atttaaaaag 4800 atgcgggcag gagttttttg cttttaataa gctcttattt ttgagtactc tgggggggat 4860 tgaccttgga catagtgtat tttgagatga tagaaaactt caaagaagac aggtgcattg 4920 tatgccatct taaaaacagg gcaatggata agttttttga tgattttctg tatgaatcag 4980 taaacgacta cagcttacgt gacagaataa gaaagggtgg aatttgtcca gggcatgcaa 5040 gaaagttgga gtcgtttgga gatgtgcttg cccatgccat tatatattcg gatttgctga 5100 gaagtttcaa acacaaccac catattgaga tattgcctcg caaaaagaaa actcaaggac 5160 aaaatatttg cgtcttttgt gaaaaagaac aaggctttga agacatctac acaagagcat 5220 tttcctactt ttttttcact tcacctcaat ttaaatctgc atttgctgaa agaggtttta 5280 tatgtcagag gcacttaaat caagttctgg aaaagacagc ttctgtatct gctaaaagag 5340 agcttttgtc agttttagaa tccaagatag acaaaatctt gaatcatctt gagagaataa 5400 aagaaaaaaa tgattatcga aatattcaca taaactatac agctgatgaa atcagggcat 5460 ggcacatggc tgttgagttt gtggcaggta cacagtagtt tattatcaga cacgcaaata 5520 ttttacaata atgataaaat gaggtgattt aggatgagga ctatagaata tgatgttgtg 5580 caagatatga ttagaaactt tactgagcaa ctccaggaga tttcaaatga aatcaattca 5640 gaatctatta aaaagttagc agggagtatt aaggaaaaaa ttgaaaagaa tgcattttat 5700 ctggttgtat tgggtcagtt taagagggga aaatcgacgc ttataaatta catgcttggt 5760 gcaaatttac taccaaccgg agttcttcct ttaacctcag taataacaaa gatttattac 5820 agtccggagg ttaaagtgga tgtaattttt gaaagtggtg taaaaaaaga aattccagtt 5880 gatgaacttg atctatattg tacagaaaga gggaatccta aaaatcaaaa atgtgttgat 5940 acaattgaga taggttatcc ctttgatttt ttgaataaag atgttgtaat tgttgacaca 6000 cctggaattg gttctgtata tcagcacaac acagatgtga catatgaatt tattgacaag 6060 tcagatgcgg ttgtgtttgt actgtctgtt gacccaccaa ttactgaagt tgaaaaacag 6120 tttctactca agattgctga aaatgtagat aaaatattct ttgtaattaa caaatctgac 6180 ttgacaagca aaaatgaaat agaagagata gtgagtttta caactaatgt aatcaaagat 6240 attacaaaaa aaggaaatat aaatatcttt cctctttcag caaagatggc acttgaaggg 6300 aagatatcta aaaatgaaga aatgattgaa aagagctgcg tagaaatttt tgagaaagaa 6360 ttaaaacaat ttttaaagga agagaaggga aaaatacaaa tcttgagcaa tctaaaaagc 6420 ttagatggtt ttttgggagt ttgcgaagcc tttttagaaa atgatatgaa actcaagatt 6480 atgcctgtaa agcaacttga agaaaatatt gaaaaattta atgagttttt ggagagagta 6540 aatcaaaaca aaattgaaat ttataagcta tttaaaattg agatgaatga tattttacaa 6600 agttttgatg acgagatgga gaaaataaag aaggaacttg tagttaagat aactaaaaag 6660 ataaatgatt attatccatc agttgcaaga cttaaaagaa ttgaacaaaa agagcatctt 6720 aataaatatc ttgaaaaggc tattgtggaa gagtttgatc 6760 // |
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