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EBI DbfetchID L17342; SV 1; linear; genomic DNA; STD; PRO; 4100 BP. XX AC L17342; XX DT 11-JUN-1993 (Rel. 36, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 8) XX DE Haemophilus parainfluenzae Valyl-tRNA synthetase (valS) gene, 3' end; HpaII DE very-short-patch-repair endonuclease (hpaIIV) gene, complete cds; HpaII DE modification methyltransferase (hpaIIM) gene, complete cds; HpaII DE restriction endonuclease (hpaIIR) ge. XX KW hpaIIM gene; hpaIIR gene; hpaIIV gene; modification methyltransferase; KW restriction endonuclease; valS gene; Valyl-tRNA synthetase; KW very-short-patch-repair endonuclease. XX OS Haemophilus parainfluenzae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Haemophilus. XX RN [1] RP 1-4100 RX DOI; 10.1093/nar/18.6.1377 RX PUBMED; 2183189. RA Card C.O., Wilson G.G., Weule K., Hasapes J., Kiss A., Roberts R.J.; RT "Cloning and characterization of the HpaII methylase gene"; RL Nucleic Acids Res. 18(6):1377-1383(1990). XX RN [2] RP 1-4100 RA Barsomian J.M., Zhang B., Noren C., Wilson G.G.; RT "Vsr-like genes associated with bacterial restriction-modification RT systems"; RL Unpublished. XX RN [3] RP 1-4100 RX DOI; 10.1016/0378-1119(94)90348-4 RX PUBMED; 7514149. RA Kulakauskas S., Barsomian J.M., Lubys A., Roberts R.J., Wilson G.G.; RT "Organization and sequence of the HpaII restriction-modification system and RT adjacent genes"; RL Gene 142(1):9-15(1994). XX FH Key Location/Qualifiers FH FT source 1..4100 FT /organism="Haemophilus parainfluenzae" FT /mol_type="genomic DNA" FT /db_xref="taxon:729" FT CDS <1..1137 FT /codon_start=1 FT /transl_table=11 FT /gene="valS" FT /product="Valyl-tRNA synthetase" FT /function="attaches valine to valyl-tRNA" FT /note="tentative: identity based on homology to FT characterized ValS proteins:X05891/J03497/L04574; M64703; FT M16318;M98326/S54592/X59303; putative" FT /db_xref="GOA:P36432" FT /db_xref="HSSP:1IVS" FT /db_xref="InterPro:IPR019499" FT /db_xref="UniProtKB/Swiss-Prot:P36432" FT /protein_id="AAA20479.1" FT /translation="DGISLEDLLEKRTGNMMQPQLAEKIAKATRKEFVDGIAAHGTDAL FT RFTLAALASNGRDINWDMKRLEGYRNFCNKLWNASRFVLTNDKLDLSQGEIEFSVADRW FT IQSEFNRTVESFRSALSQYRFDLCANAIYEFTWNQFCDWYLELTKPVFANGNAAQIRAA FT SQTLVHVLEKLLRLAHPLIPFITEEIWQKVKGFVGITADSIMLQPFPQVEENGFDPEAE FT AEIEWLKEVIVAVRNIRAESNIAPSKGLDLLFRNLSAENAKILEKQTALLKAMAKLDNV FT QVLAANETHASVAKLVGNAELLVPMAGFINKEAELARLTKEIEKYQNEVKRIENKLSNE FT AFVAKAPKAVITKEREKQAEYQSGLEKIQEQYKAIEAL" FT CDS 1188..1613 FT /codon_start=1 FT /transl_table=11 FT /gene="hpaIIV" FT /standard_name="V.HpaII" FT /product="HpaII very-short-patch-repair endonuclease" FT /function="speculated to nick HpaII sequences that contain FT T:G mispairs resulting from m5C-deamination" FT /note="tentative: identity based on homology to the E.coli FT Vsr protein:X13330" FT /db_xref="GOA:P36434" FT /db_xref="HSSP:1VSR" FT /db_xref="InterPro:IPR004603" FT /db_xref="UniProtKB/Swiss-Prot:P36434" FT /citation=[2] FT /protein_id="AAA20480.1" FT /translation="MDKLTPEQRKKCMKASSKNKGTKPELLLAKYLWALGLRYRKNDRS FT IFGTPDLSFKRYKIAIFIDGEFWHGKDWDIRKYDIKSNKDFWISKIEHNMNRDKKVNDY FT LISNGWVIFRFWGKDVLKNPEKFSLEIQKAIYERCVR" FT RBS 1585..1587 FT /gene="hpaIIV" FT /note="putative" FT CDS 1594..2670 FT /codon_start=1 FT /transl_table=11 FT /gene="hpaIIM" FT /standard_name="M.HpaII" FT /product="HpaII modification methyltransferase" FT /function="methylates ds DNA at CCGG forming Cm5CGG. FT Requires S-adenosylmethionine as co-factor." FT /note="member of the m5C-class of DNA methyltransferases" FT /db_xref="GOA:P15446" FT /db_xref="HSSP:6MHT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P15446" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA20481.1" FT /translation="MKDVLDDNLLEEPAAQYSLFEPESNPNLREKFTFIDLFAGIGGFR FT IAMQNLGGKCIFSSEWDEQAQKTYEANFGDLPYGDITLEETKAFIPEKFDILCAGFPCQ FT AFSIAGKRGGFEDTRGTLFFDVAEIIRRHQPKAFFLENVKGLKNHDKGRTLKTILNVLR FT EDLGYFVPEPAIVNAKNFGVPQNRERIYIVGFHKSTGVNSFSYPEPLDKIVTFADIREE FT KTVPTKYYLSTQYIDTLRKHKERHESKGNGFGYEIIPDDGIANAIVVGGMGRERNLVID FT HRITDFTPTTNIKGEVNREGIRKMTPREWARLQGFPDSYVIPVSDASAYKQFGNSVAVP FT AIQATGKKILEKLGNLYD" FT RBS 2652..2655 FT /gene="hpaIIM" FT /note="putative" FT CDS 2663..3739 FT /codon_start=1 FT /transl_table=11 FT /gene="hpaIIR" FT /standard_name="R.HpaII" FT /product="HpaII restriction endonuclease" FT /function="cleaves ds DNA at CCGG, forming C'CGG" FT /db_xref="GOA:P36433" FT /db_xref="InterPro:IPR019062" FT /db_xref="UniProtKB/Swiss-Prot:P36433" FT /citation=[3] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA20482.1" FT /translation="MTEFFSGNRGEWSEPYALFKLLADGQLYLGDSQLNKLGIVMPILS FT ILRQEKNYESSYILHNNSQNIIVTYNNEKFTVPISGFQEKAVLLLSEIKNASGNRAFSI FT PSIDDFLKKLGFTHLSASSSSKSDIHIVVHDLRTGITPTLGFSIKSQLGSPATLLNASK FT ATNFTFKIYNLKDKQIEYINSLSGIKEKIKEIFSQDGKLEFVKVESCKFSNNLTLIDTK FT LPEILAEMILLYYSSKLNKIDDVTEHISRLNPLNYNLSCNHNYYEYKVKHFLNDVALGM FT RPDDVWLGQYDATGGYLVVKEDGELLCYHIYSKNSFEDYLYCNTKFDTPSSSRHDFGHI FT YQVNHDFFIKLNVQIRFL" XX SQ Sequence 4100 BP; 1387 A; 620 C; 812 G; 1281 T; 0 other; gacggtatca gccttgaaga tttacttgaa aaacgtaccg gcaacatgat gcaaccgcaa 60 ttggcagaga aaattgctaa agcaacgcgc aaagaatttg tggacggcat tgccgctcac 120 ggtacagatg cattacgttt cacattagcg gcattagctt caaacggccg tgacatcaac 180 tgggatatga aacgcttgga aggttaccgc aacttctgta ataaattgtg gaatgcaagc 240 cgttttgtgc tcaccaatga caaattagat ttaagccaag gcgaaattga attttccgtg 300 gcagaccgtt ggattcaatc tgaattcaat cgcacagtag aatcattccg cagtgcatta 360 agccaatatc gtttcgatct ttgtgccaat gcgatttatg agtttacctg gaatcaattc 420 tgcgactggt atttagaatt gactaaacca gtatttgcaa acggcaatgc agcacaaatc 480 cgtgcggcaa gccaaacctt ggttcacgtg ctagaaaaat tattacgttt agcacatccg 540 cttattccat ttattaccga agaaatttgg caaaaagtga aaggatttgt aggcattacg 600 gctgacagca ttatgttaca acctttccca caagtggaag aaaacggctt tgatccagaa 660 gcagaagctg aaattgagtg gttaaaagaa gtgattgttg cggtgcgtaa tattcgtgca 720 gaaagcaaca ttgcaccaag caaaggctta gatctgttat tccgtaattt aagcgcagaa 780 aacgcaaaaa ttctcgaaaa acagaccgct cttttaaaag ccatggcgaa gttagacaac 840 gttcaagtgt tagcagcaaa cgaaacgcac gcttcggtag cgaaactcgt cggcaatgcg 900 gaattactcg tgccaatggc tggctttatc aataaagaag cagagcttgc tcgtttaacc 960 aaagagattg aaaaatatca aaatgaggtt aaacgtatcg aaaacaaact cagcaatgaa 1020 gctttcgtgg ctaaagcacc taaagcggtg attacgaaag aacgtgaaaa acaagcagaa 1080 tatcaatctg gattagaaaa aatccaagag cagtataaag cgattgaagc gttgtagtta 1140 gtcttatttg gttgctttaa atttactgtt taaacagtat atcagctatg gataaattaa 1200 ctcctgagca acgtaaaaaa tgtatgaaag ctagtagtaa gaataaaggg acaaagcctg 1260 agttactact agctaaatat ttatgggctt taggattaag atatcgtaag aatgatagaa 1320 gcatttttgg tactcccgat ttaagtttta aaaggtacaa aattgcaatt tttattgatg 1380 gtgagttttg gcatggaaag gattgggata ttcgaaagta tgatatcaaa agtaacaaag 1440 atttttggat ctcaaaaatt gagcataata tgaataggga taagaaagta aacgattatc 1500 ttatttctaa tggttgggta atatttcgat tttggggaaa ggatgtatta aaaaatcctg 1560 agaagtttag tttagagata cagaaagcaa tttatgaaag atgtgttaga tgataacttg 1620 ttagaagaac ccgctgcaca atatagttta tttgaaccag agtccaaccc taatttacga 1680 gaaaaattta cttttattga tttatttgca ggtattggtg gattccgcat tgccatgcaa 1740 aatttaggag gtaaatgcat tttctctagt gaatgggatg agcaagctca gaaaacttat 1800 gaggctaatt ttggtgattt gccttatgga gatattacct tagaggaaac aaaggctttt 1860 attcctgaaa aatttgatat tttatgtgct ggttttcctt gtcaggcatt ttctattgca 1920 ggaaaacgtg gaggatttga agatactaga gggactttgt tttttgatgt tgcagaaatt 1980 atacgtcgtc atcagcctaa agcatttttt ttagagaatg taaaaggatt aaaaaaccat 2040 gataaaggta ggacattaaa aactatattg aatgtactaa gagaagattt aggttatttt 2100 gttcctgaac cagcaattgt taatgctaag aattttggtg tgccacaaaa tagagaaaga 2160 atttatattg taggctttca taaaagcact ggtgttaata gttttagtta tccagaacct 2220 ttagataaaa ttgtaacttt cgctgatatt cgggaagaaa aaacagttcc aactaaatat 2280 tacctatcaa ctcagtatat tgatacttta agaaaacata aagaacgtca tgagagtaaa 2340 ggtaatggtt ttggttatga aattattcca gatgatggaa tagccaatgc gattgtagtt 2400 ggaggtatgg gacgtgaacg taatcttgta attgatcata gaattacgga ttttactcct 2460 actacgaata ttaaagggga ggtaaatcgt gaagggattc gtaaaatgac ccctcgagaa 2520 tgggcaagat tgcaggggtt tccagatagt tatgttattc cggtttctga tgcatcagcg 2580 tataaacaat ttggtaattc agtagcagtg ccggctattc aagctacagg taagaaaatt 2640 ttagaaaaat taggaaattt atatgactga atttttttct ggtaatagag gagagtggag 2700 tgagccttac gccctcttta agttattggc tgatggtcag ctttatttag gagatagtca 2760 actaaataaa ctgggaattg taatgccgat tctatcaatt cttcggcagg aaaaaaatta 2820 tgagagttca tatatacttc ataacaattc tcaaaatatt atagttacat ataataatga 2880 aaaatttaca gttccaattt ccggatttca agaaaaagct gttttgctgt tatcagaaat 2940 aaaaaatgca tcaggcaata gggctttttc tatcccgagt attgatgatt ttctaaagaa 3000 gcttggtttc actcatctta gtgcaagctc ctcatctaaa agcgatattc atattgttgt 3060 tcatgattta agaacgggga ttacgcctac tttgggattt agtattaagt ctcaactggg 3120 aagccctgca acacttttaa atgcaagtaa agcaaccaac tttacgttca aaatatataa 3180 tctaaaagat aagcaaattg aatatattaa ttctcttagc gggataaagg aaaagataaa 3240 agaaattttt tctcaagacg gtaaattaga atttgttaaa gttgaaagct gtaagttttc 3300 taataatctt acattaatag ataccaaatt acctgagatt ttagctgaaa tgatattgct 3360 ttattattct tctaagctca ataaaataga tgatgtaacg gaacatatta gtagattaaa 3420 tccattaaat tataatttat cttgtaacca taattattat gaatataaag taaaacactt 3480 tcttaatgat gtcgctttag gtatgcgtcc cgatgatgtt tggttagggc agtatgatgc 3540 aacaggcgga tatctggtag taaaagaaga tggagagctt ctttgttatc atatttatag 3600 taagaattct tttgaagact atttatattg caataccaaa tttgatacac ctagttcatc 3660 aaggcatgat tttggacata tttatcaagt aaatcatgat ttttttatta agttaaacgt 3720 acaaattaga ttcttataat caaagtgcgg taagttttat ccgcactttt taattatttc 3780 tttattaagc ccttctaact ttctacaaat tatttgtaag taaaaattga tgctagttat 3840 ttaatatctc aagcaatgct tgcacaggat gtttcaagat ggctttttca tatcgcttca 3900 cttggctacg gcaggaatag ccggttgcga gacaatgatt tggatcttta ccttggtaat 3960 tttttgcccc atgatacgtc gtaaatatct tttcgatatg gtttgatttt gaacctcatg 4020 accaaataca cccgccatgc cacagcagcc gactttttca acattcaagg tttgtccaaa 4080 ttgcgcaaaa atatgctgcc 4100 // |
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