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EBI DbfetchID L17341; SV 1; linear; unassigned DNA; STD; PRO; 3454 BP. XX AC L17341; XX DT 05-JUN-1993 (Rel. 36, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 6) XX DE Bacillus coagulans restriction endonuclease A and B subunit (bcgIA and DE bcgIB) genes, complete cds. XX KW bcgIA gene. XX OS Bacillus coagulans OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3454 RX DOI; 10.1093/nar/21.4.987 RX PUBMED; 8451198. RA Kong H., Morgan R.D., Maunus R.E., Schildkraut I.; RT "A unique restriction endonuclease, BcgI, from Bacillus coagulans"; RL Nucleic Acids Res. 21(4):987-991(1993). XX RN [2] RP 1-3454 RX PUBMED; 8276869. RA Kong H., Roemer S.E., Waite-Rees P.A., Benner J.S., Wilson G.G., RA Nwankwo D.O.; RT "Characterization of BcgI, a new kind of restriction-modification system"; RL J. Biol. Chem. 269(1):683-690(1994). XX FH Key Location/Qualifiers FH FT source 1..3454 FT /organism="Bacillus coagulans" FT /mol_type="unassigned DNA" FT /db_xref="taxon:1398" FT RBS 160..164 FT /gene="bcgIA" FT /note="putative" FT CDS 173..2086 FT /codon_start=1 FT /transl_table=11 FT /gene="bcgIA" FT /product="restriction endonuclease alpha subunit" FT /function="Inactive alone. Together with the B subunit, FT forms a complex that restricts and modifies the dsDNA FT sequence CGAN6TGC. Probably functions as the FT methyltransferase subunit (M)" FT /note="codons 1-14 confirmed by N-terminal amino acid FT sequence analysis of the purified A protein; FT s-adenosylmethionine is required for methylation. FT Methylation occurs within the recognition sequence and FT converts A to m6A" FT /db_xref="GOA:Q07605" FT /db_xref="InterPro:IPR003356" FT /db_xref="UniProtKB/Swiss-Prot:Q07605" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA16626.1" FT /translation="MVNEKTSTDQLVRRFIEDLGVTYEEQGSSNIQIKQALRSASKSKS FT QSGVGKPEFIFFSGNHLIIVEDKLAIDKLEYKNNDGIIDTEFPFRRDYAVNGAVHYARH FT IIEKTNSYKEVIAIGIAGDGLHYQIHPYFVSETELKKLPEMKSLEDISPENIEEFYKVA FT VLGELPKEERELREVNKIAADMHEDLRNYGQLEGEKKATVVSAILLALEEPTFSLNQLI FT GSDRVGGTDGEIIFNAVRVYLENAGIVPYAKVGEMLDQFIFIQRDVTLNTVNSNLEMTP FT LKYFATTLEAEIMDKIKSNTDFDILGNFYGEFVKYGGNDGNPLGIVLTPRHITSLMAEL FT IGINKSDFVLDPACGTGAFLISAMNRMLGQAENDDERRDIKQNRLYGIEIQQKLFTIAT FT TNMILRGDGKSNLIRDNCLTFDNTIMNGYGINKILMNPPYSQAKNDQTQHLSELSFIQQ FT ALEMLVVGGKLCAIVPQSTMVGKNRHDKARKKQILKQHTLETVITLNKDTFHGVGVNPC FT IVIFKAGIKHPENKRVSFVNFEDDGHVVRKHVGLVGDGTEKGKREHLLAVLAGDEDDGT FT DLIVKTAIKDTDEWLHSFYYFNDGIPSEDDFYKTVANYLTFQFDMTANGKGYLFEGVEE FT NE" FT RBS 2064..2069 FT /gene="bcgIA" FT /note="putative" FT CDS 2079..3104 FT /codon_start=1 FT /transl_table=11 FT /gene="bcgIB" FT /product="restriction endonuclease beta subunit" FT /function="Inactive alone. Together with the A subunit, FT forms a complex that restricts and modifies the dsDNA FT sequence CGAN6TGC. Probably functions as the endonuclease FT subunit (R)" FT /note="codons 1-18 confirmed by N-terminal amino acid FT sequence analysis of the purified B protein; FT s-adenosylmethionine is required for cleavage. ds cleavage FT occurs on both sides of the recognition sequence and FT excises a 34-bp fragment,thus: N10/12 CGAN6TGC N12/10" FT /db_xref="GOA:Q07606" FT /db_xref="InterPro:IPR011634" FT /db_xref="UniProtKB/Swiss-Prot:Q07606" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA16627.1" FT /translation="MNNLIKYSTFLISDLFDVVIGKTIDGNKAQRNENGTPYITRKATR FT NGFEFMIDGEKEKLYSGKLPVITIGNETSKPFVQEFHFFTGTKVNICIPKLDLNRNHLL FT YITTMIENATKMFSYSYTINSTRLKSLKILLPIKGEEPDWDYMNTYISKILSNMEKNFD FT VQQNDGVSDLRSLKDLSWSQFKMDEIFSINSGVRLTKADMKPGNIPFIGATDSNNGITE FT FTSSTNASFDGNVLGVNYNGSVVENFYHPYKAVFSDDVKRLKLKNYPNNKHVLLFMKVV FT ILQQKVKYAYGYKFNATRMKEQIILLPTKADGTPDYEFMEQYMMRMENKVVGRTTEKEA FT D" XX SQ Sequence 3454 BP; 1241 A; 507 C; 665 G; 1041 T; 0 other; gttaacgtaa aaagagctac tcatcttgta aggaaaaagc ctccactctt acatgaatag 60 agacttttta catattgata atttgctgtt tgttctagct acgtattgtg cactcggcta 120 atctaattta ttattattaa tagttgttca ataataggca ggtgggaata tcatggtaaa 180 cgaaaagaca agtacagatc aacttgtaag aagatttata gaagacttag gtgttacata 240 tgaagaacaa ggatcctcga acattcaaat caaacaagcc ctaagaagcg caagtaaatc 300 caagagccaa tcaggtgtgg gtaaaccaga gtttattttc ttttctggta atcatttaat 360 tattgtagaa gataagctag cgatagataa gcttgaatat aaaaataatg atgggatcat 420 tgataccgag ttcccgttta gaagagatta tgctgttaat ggcgcggttc attatgcaag 480 acatatcatt gaaaaaacta attcatataa agaagttatc gcaataggaa ttgcagggga 540 tggactacat tatcaaatac atccctattt tgtgtcggag acagagctga aaaaattacc 600 tgaaatgaaa agcttagagg atatttctcc tgaaaatata gaagaatttt ataaagtagc 660 cgtattgggg gaacttccaa aagaagaacg tgaattaaga gaagtaaata agattgctgc 720 ggatatgcat gaagatttac gaaattatgg acagctcgaa ggtgaaaaaa aagcgacagt 780 cgtctcggcg attctacttg ctctagaaga acctaccttt tctttaaatc aattaattgg 840 ttcagatcga gttggaggta cggatggtga aattattttc aacgccgtaa gggtgtacct 900 tgagaatgct ggtatcgtcc cctatgcaaa agttggtgaa atgttagacc agtttatttt 960 tattcaaagg gatgtaactt tgaatacagt caattcaaac cttgaaatga cacctctcaa 1020 atactttgct actactcttg aagcagaaat tatggacaaa atcaagagca atacagactt 1080 tgatatactt ggaaatttct atggtgagtt tgtaaagtac ggtggtaatg atggtaaccc 1140 tttaggaatt gttttaacac caagacatat aacgtcttta atggcagaac taattggtat 1200 aaataaatca gattttgttt tagaccctgc atgtggtacg ggcgcctttt taattagtgc 1260 catgaaccga atgcttggac aagccgaaaa tgatgacgaa aggcgagata ttaaacaaaa 1320 tcgtttatat ggtattgaaa ttcaacagaa gcttttcact attgcaacaa caaacatgat 1380 tcttcgaggg gatggaaaaa gtaatcttat acgtgataat tgcttaacgt ttgacaatac 1440 tattatgaat ggatatggga ttaataaaat actcatgaac cctccatact cccaagcgaa 1500 aaatgaccaa acacaacatt tatcggaact aagctttatt cagcaagctt tagagatgtt 1560 agtagttggg gggaaattat gtgcgatagt acctcaatca actatggtcg ggaaaaatcg 1620 acacgataaa gctagaaaga agcaaatatt aaaacaacat acattagaaa cagttatcac 1680 tctaaataaa gatactttcc acggcgtagg cgttaatccc tgtattgtta tttttaaagc 1740 aggaattaag catcctgaaa acaagagagt gtcatttgta aactttgaag atgatggcca 1800 tgtagtgaga aaacacgttg gtcttgtagg agatggaaca gaaaaaggga aaagagagca 1860 tctacttgca gttcttgcag gagatgaaga tgatggtaca gatcttattg taaagaccgc 1920 aataaaggac actgatgagt ggttacacag tttctattac tttaatgatg ggattccttc 1980 agaagatgat ttttacaaaa cagttgctaa ttatttgact tttcagtttg atatgactgc 2040 taatggaaag ggttatctgt ttgaaggagt agaagaaaat gaataatctg attaaatatt 2100 cgactttttt aatttctgat ctattcgatg tagttattgg aaagactatt gacggcaata 2160 aagcccagag aaacgaaaat ggaacgcctt atatcacaag aaaagctaca agaaatggtt 2220 ttgaatttat gatagatggt gaaaaggaaa aactctatag tggtaagttg cctgttatta 2280 ctattggtaa tgaaaccagc aagccttttg ttcaagaatt tcattttttt acaggcacaa 2340 aagtaaatat atgcatccca aaattagatt tgaataggaa ccatttactg tatataacga 2400 caatgataga aaatgcaaca aaaatgttta gttattcata tactataaat tcaactcggt 2460 taaagagttt aaaaatactt ttaccaatta agggagaaga gcccgattgg gattatatga 2520 acacttatat ttccaagatt cttagtaata tggagaaaaa ttttgacgtt caacaaaatg 2580 atggggtttc ggatctaagg tcattaaaag acttatcatg gagtcagttc aagatggatg 2640 aaattttttc aatcaatagt ggagtgagat taacaaaagc tgatatgaaa cccggaaaca 2700 tcccattcat aggggctact gatagcaaca acggtataac ggaatttacc tctagcacaa 2760 atgccagctt tgacggtaat gtcttaggcg taaattataa cggtagtgtt gtagaaaatt 2820 tctatcaccc ttataaggcg gttttctcgg atgatgttaa acgtcttaag cttaagaatt 2880 atccgaataa caaacatgtt ctattgttta tgaaagtcgt aatccttcaa caaaaagtga 2940 aatatgcata tggctataaa tttaatgcca cacgaatgaa ggagcaaata atacttcttc 3000 caacaaaagc ggacggtaca ccggactatg agttcatgga acaatatatg atgcgtatgg 3060 agaataaagt agttggaaga accacagaaa aagaagcaga ttaataacga tctgcttctt 3120 tttctattac tataattagt tttttttatt ggattagcaa ttgcaacaat caaattgcta 3180 aaaaggatcc ataagaagcc caaaaacatt ccaaattagt gttatcgtaa aatccttgtt 3240 tatcactctc aacccctttc atttttgaaa atctcaactc tattttagat aagattattt 3300 aagttatatt ttagaactta ttcgttgttt attaactaga agtgtatcaa tttattagat 3360 tttcattttt ttagtaaagg ctagcttgtg aaataatgta cttaaagaaa cgaggcgtta 3420 attcaataag ctaaaaagag cctttagcgg tccg 3454 // |
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