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EBI DbfetchID L17309; SV 1; linear; genomic DNA; STD; PRO; 6300 BP. XX AC L17309; XX DT 27-NOV-1993 (Rel. 37, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 11) XX DE Bacillus subtilis homologues of motA and motB genes acetoin utilization DE operon genes acuA, acuB and acuC, and acetyl-coA synthase (acsA) genes, DE complete cds. XX KW acetoin utilization protein; acuA gene; acuB gene; acuC gene; homologue; KW motA gene; motB gene. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6300 RX PUBMED; 1735721. RA Henkin T.M., Glass B.L., Grundy F.J.; RT "Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory RT sequence in multiple tRNA synthetase genes"; RL J. Bacteriol. 174(4):1299-1306(1992). XX RN [2] RP 1-6300 RX DOI; 10.1111/j.1365-2958.1993.tb01952.x RX PUBMED; 7934817. RA Grundy F.J., Waters D.A., Takova T.Y., Henkin T.M.; RT "Identification of genes involved in utilization of acetate and acetoin in RT Bacillus subtilis"; RL Mol. Microbiol. 10(2):259-271(1993). XX DR RFAM; RF00230; T-box. XX CC On Jun 14, 1995 this sequence version replaced gi:348047. XX FH Key Location/Qualifiers FH FT source 1..6300 FT /organism="Bacillus subtilis" FT /map="263 degrees" FT /strain="W168" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS 31..849 FT /codon_start=1 FT /transl_table=11 FT /function="unknown; similar to motA product" FT /label=ORFA FT /note="putative" FT /db_xref="GOA:P39063" FT /db_xref="InterPro:IPR000540" FT /db_xref="InterPro:IPR002898" FT /db_xref="UniProtKB/Swiss-Prot:P39063" FT /citation=[2] FT /protein_id="AAA68282.1" FT /translation="MKRFDYLTPVGFVLGTIIIVIGIISGSGVSGFRSFLDLTSFFIVT FT GGLCAAVFISFPPSELKKAPSVLKQAFIRQEDNVKDLVKTFVSLSDHARKHGLLSLDDQ FT AREIKDPFLKKGLLLAIDGWDEETIRLVMDSEIAAMEERHRKGRRVFEKAGEFAPAWGM FT IGTLVGLVLMLKNLNDPHMLGPNMAIALLTTLYGSLLANMVFNPIAAKLEEKTESEIFI FT KQVMVEGIIGVQSGKNPRNLESQLVVFSSREEWQKQPKQVKTKKGSVHEA" FT CDS 839..1567 FT /codon_start=1 FT /transl_table=11 FT /function="unknown; similar to motB product" FT /label=ORFB FT /note="putative" FT /db_xref="GOA:P39064" FT /db_xref="InterPro:IPR006665" FT /db_xref="UniProtKB/Swiss-Prot:P39064" FT /citation=[2] FT /protein_id="AAA68283.1" FT /translation="MKLRRERFERRNGSGKNSQSSSSWMVTFTDLITLILVFFILLFSM FT SQIDLQKFKAAVDSIQKEGNGLQPDQTSIEKKNTSPSDTKKQEDQQDQLLKKVNTYIKD FT NHLKAQMTAKRDERGVVLVLQEAVLFDTGEAKVLKNAETLLHQIAVLLQTIPNDIQVEG FT HTDSRNISTYRYPSNWELSAARASGVIQYFTSKEKLPSKRFIAVGYADTKPVKDNKTNE FT HMKENRRVEIVIKKSKTTSS" FT terminator 1549..1578 FT /note="putative" FT /citation=[2] FT CDS complement(1578..2741) FT /codon_start=1 FT /transl_table=11 FT /gene="acuC" FT /product="acetoin utilization protein" FT /note="putative" FT /db_xref="GOA:P39067" FT /db_xref="InterPro:IPR000286" FT /db_xref="InterPro:IPR003085" FT /db_xref="UniProtKB/Swiss-Prot:P39067" FT /citation=[2] FT /protein_id="AAA68284.1" FT /translation="MRDSVFIYSPSYQTYMFHQEHPFNQQRVLLTYDLLKTINAFDDGD FT IVTPRLASEEELSLVHTDDYIQAVKLAGAGKLPAEEGESYGLGTEDTPVFAGMHEAASL FT LVGGTLTAADWVMSGQALHAANLGGGLHHGFRGRASGFCIYNDSAVAIQYIQKKYSARV FT LYIDTDAHHGDGVQFTFYDNPDVCTLSIHETGRYLFPGTGQIQEKGSGKGYGYSFNIPL FT DAFTEDDSFLEAYRTAASEVAAYFEPDVIISQNGADAHYYDPLTHLSATINIYEEIPRL FT AHTLAHQYCGGKWIAVGGGGYDIWRVVPRAWARIWLEMKGIDPGHEIPPEWIVKWQKQC FT PVALPSSWSDPADLYPPIPRKPEITEKNAQTVSKALYAIRSEQQRTK" FT CDS complement(2738..3382) FT /codon_start=1 FT /transl_table=11 FT /gene="acuB" FT /product="acetoin utilization protein" FT /note="putative" FT /db_xref="GOA:P39066" FT /db_xref="InterPro:IPR000644" FT /db_xref="InterPro:IPR002912" FT /db_xref="UniProtKB/Swiss-Prot:P39066" FT /citation=[2] FT /protein_id="AAA68285.1" FT /translation="MIVEQIMKRDVITLTKTDTLETAICKLKEFHIRHLPVVDEERHVI FT GMITDRDMKQASPSIFEENKRSLFLTRSVDSIMKKDVVCAHPLDFVEEISAVFYEHGIG FT CLPVVHHQKLIGILTKTDLLRTFVKLTGADQPGSQIEIKVNDITKSLAEISSLCQDLQV FT KILSVLVYPHDDPGVKVLVFRVKTMNPLPFLQALQRNGHHVVWPSEQRDLL" FT CDS complement(3409..4041) FT /codon_start=1 FT /transl_table=11 FT /gene="acuA" FT /product="acetoin utilization protein" FT /note="putative" FT /db_xref="GOA:P39065" FT /db_xref="InterPro:IPR000182" FT /db_xref="InterPro:IPR016181" FT /db_xref="UniProtKB/Swiss-Prot:P39065" FT /citation=[2] FT /protein_id="AAA68286.1" FT /translation="MEHHKTYHSANIKTATGSLLIEGPVSPEDLAGYEFHKDLTAFRPP FT REQHEALVDIAGLPEGRIIIARDGRTIVGYVTYLYPDPLERWSEGNMEDLIELGAIEVA FT PDYRGCAVGKTLLTVSMMDEQMENYIVMTTEYYWHWDLKGMKKDVWEYRKIMEKMMNAG FT GLVWFATDEPEISSHPANCLMARIGKNVSQESIEQFDRLRFYHRYMY" FT -10_signal complement(4089..4094) FT /note="acuABC promoter sequence" FT /citation=[2] FT -35_signal complement(4111..4116) FT /note="acuABC promoter sequence" FT /citation=[2] FT -35_signal 4137..4142 FT /note="acsA promoter sequence" FT /citation=[2] FT -10_signal 4160..4165 FT /note="acsA promoter sequence" FT /citation=[2] FT CDS 4202..5920 FT /codon_start=1 FT /transl_table=11 FT /gene="acsA" FT /product="acetyl-CoA synthetase" FT /function="acetate utilization" FT /db_xref="GOA:P39062" FT /db_xref="InterPro:IPR000873" FT /db_xref="InterPro:IPR020845" FT /db_xref="UniProtKB/Swiss-Prot:P39062" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA68287.1" FT /translation="MNLKALPAIEGDHNLKNYEETYRHFDWAEAEKHFSWHETGKLNAA FT YEAIDRHAESFRKNKVALYYKDAKRDEKYTFKEMKEESNRAGNVLRRYGNVEKGDRVFI FT FMPRSPELYFIMLGAIKIGAIAGPLFEAFMEGAVKDRLENSEAKVVVTTPELLERIPVD FT KLPHLQHVFVVGGEAESGTNIINYDEAAKQESTRLDIEWMDKKDGFLLHYTSGSTGTPK FT GVLHVHEAMIQQYQTGKWVLDLKEEDIYWCTADPGWVTGTVYGIFAPWLNGATNVIVGG FT RFSPESWYGTIEQLGVNVWYSAPTAFRMLMGAGDEMAAKYDLTSLRHVLSVGEPLNPEV FT IRWGHKVFNKRIHDTWWMTETGSQLICNYPCMDIKPGSMGKPIPGVEAAIVDNQGNELP FT PYRMGNLAIKKGWPSMMHTIWNNPEKYESYFMPGGWYVSGDSAYMDEEGYFWFQGRVDD FT VIMTSGERVGPFEVESKLVEHPAIAEAGVIGKPDPVRGEIIKAFIALREGFEPSDKLKE FT EIRLFVKQGLAAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLPAGDLSTMED" FT -35_signal 5930..5935 FT /gene="tyrS" FT /citation=[1] FT /citation=[2] FT -10_signal 5953..5958 FT /gene="tyrS" FT /citation=[1] FT /citation=[2] FT CDS 6262..>6300 FT /codon_start=1 FT /transl_table=11 FT /gene="tyrS" FT /db_xref="GOA:P22326" FT /db_xref="InterPro:IPR001412" FT /db_xref="InterPro:IPR002305" FT /db_xref="InterPro:IPR002307" FT /db_xref="InterPro:IPR002942" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P22326" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA68288.1" FT /translation="MTNLLEDLSFRGL" XX SQ Sequence 6300 BP; 1748 A; 1355 C; 1535 G; 1662 T; 0 other; gaattcttgc ttttttcatg gggagaaatg atgaaacgtt ttgattatct tacacctgtt 60 ggatttgtgt taggaacgat tattattgtt atcgggatta tttcaggatc gggagtaagc 120 ggtttccgct cttttcttga tctgacctct ttcttcatcg taacaggggg actttgcgcc 180 gctgttttta tcagttttcc gccaagtgag ctgaaaaaag cgccctctgt gttaaagcag 240 gcattcatcc gccaggaaga caatgtgaaa gatcttgtga aaacctttgt ttccctttcc 300 gatcacgcgc gaaaacacgg gcttttatca ttggatgatc aggctcggga aatcaaagat 360 ccattcttga aaaaagggct gcttttagcg attgacggct gggatgaaga aacgattcgg 420 ctcgtcatgg attcagaaat cgcagcaatg gaggaacggc accgaaaagg gcggcgagtt 480 tttgaaaaag cgggagaatt cgctccggca tggggaatga tcggaacact cgtcgggctt 540 gtgctgatgc tcaaaaactt aaacgacccg catatgctcg gaccaaacat ggccattgcg 600 cttctgacga ctttatatgg ttcattactg gcaaatatgg tcttcaaccc gatcgcggct 660 aagcttgaag agaaaacaga aagtgaaatt ttcatcaagc aggtaatggt ggaagggatt 720 atcggtgttc aatccggaaa aaacccgcgc aaccttgaaa gccagctggt cgttttcagc 780 tcacgggaag aatggcagaa gcagccaaaa caagtaaaaa caaaaaaggg atctgtacat 840 gaagcttaga agagaacgtt ttgaaagaag gaatggaagc ggaaaaaaca gtcaatcttc 900 atccagctgg atggtaacct ttacagatct gatcaccttg atccttgtgt tctttatttt 960 actcttttcg atgtcccaaa tcgatctgca aaaattcaaa gcggcagtgg actcgatcca 1020 gaaggaaggc aacgggcttc agccggacca aacatccata gaaaaaaaga atacatcccc 1080 atctgatacg aagaagcaag aggatcagca ggatcagctt ctcaagaaag taaatacata 1140 tataaaagac aatcatttga aggcccaaat gacagccaaa cgggatgaac gcggtgtcgt 1200 gctcgttctt caggaggctg tgctgtttga tacaggtgag gcaaaggtgc tgaaaaatgc 1260 cgaaacactt cttcaccaaa ttgccgttct tcttcaaaca attccaaatg acattcaggt 1320 tgagggacat acagacagcc gaaacatttc gacataccga tacccgtcga actgggagct 1380 atcagcagca cgggcaagtg gagtgattca atacttcacg tctaaagaga agcttccctc 1440 caagcgcttt attgctgtcg gttatgcgga tacaaagcca gtaaaggata acaagacaaa 1500 tgaacacatg aaggaaaatc ggcgtgtgga aatagtgatt aaaaaatcaa aaacgacctc 1560 ttcgtaaagg tcgtttttta ctttgttctt tgctgttcag aacgtatcgc atataatgct 1620 ttgctgacag tttgggcatt tttttctgta atttcaggct tgcggggaat cggaggatat 1680 aaatcagccg gatcgctcca gctcgacgga agggccacgg ggcattgttt ttgccattta 1740 acaatccatt ccggcgggat ctcgtgaccc gggtcaattc ccttcatttc aagccatatt 1800 ctggcccagg cgcggggcac gacacgccaa atatcatatc cgccgcctcc gactgcaatc 1860 catttccctc cgcaatattg atgagccaaa gtgtgtgcaa gacgggggat ttcttcgtat 1920 atattgattg ttgctgataa atgcgtcagc gggtcgtagt aatgggcatc ggcgccgttt 1980 tggctgataa tgacatcggg ctcgaaatac gcggcgactt ctgaagctgc tgtccggtaa 2040 gcctcaagaa aagaatcatc ctctgtaaaa gcgtcaagcg gaatattaaa ggaatagcca 2100 tatccttttc cgcttccttt ttcttggatc tgaccggttc cagggaataa ataccttcct 2160 gtttcatgaa tggacagcgt gcatacgtca ggattatcgt aaaatgtaaa ttgcacaccg 2220 tccccgtggt gggcgtccgt atcaatataa agcactctgg cgctatattt tttctggata 2280 tattgaatag ccacagcgct gtcgttgtat atgcagaatc ctgatgctct cccgcgaaag 2340 ccgtgatgaa gccctccgcc aaggtttgcc gcatgcaggg cttgtcctga catgacccag 2400 tctgcggccg ttaaagtgcc gccgaccaag agtgatgccg cttcgtgcat gccggcaaag 2460 acgggtgtgt cttcagtgcc gagcccgtag ctttctcctt cttcagcggg gagctttccg 2520 gcgcccgcaa gctttacggc ctgaatgtaa tcgtccgtgt ggacaaggga aagctcctct 2580 tctgaagcga gtctgggggt gacaatgtct ccatcatcaa aggcattgat cgtcttaagc 2640 aaatcatatg ttaacagaac ccgctgctga ttaaagggat gctcctgatg aaacatatag 2700 gtttggtagg atggagaata gataaataca ctgtctctca tagcagatcc ctttgctctg 2760 acggccatac gacgtggtgt ccatttcttt gtaatgcctg caaaaacggc agcggattca 2820 tcgtttttac acggaagaca agcactttta caccgggatc gtcatgcgga taaacaagca 2880 cgcttaatat tttcacttga aggtcttggc aaagactgct gatctcagcg aggcttttgg 2940 tgatgtcgtt tacttttatc tcaatttgcg agcccggctg gtcagcgcct gtcaatttga 3000 caaacgtccg cagcaaatcg gtttttgtta aaatgcctat taatttttga tggtgtacca 3060 ctggtaggca gccgatgcca tgctcgtaga acacggctga tatttcttcg acaaaatcta 3120 acgggtgggc gcagacaaca tcctttttca taattgaatc tacgcttcgt gtgagaaata 3180 ggcttcgttt gttttcttca aaaatactcg ggctggcctg tttcatgtcc ctgtctgtaa 3240 tcatgccgat cacgtgtcgt tcttcatcta caacaggcag atgcctgata tggaattcct 3300 tcagtttgca tattgctgtt tcaagcgtat ccgtcttcgt taatgtaatg acatctcttt 3360 tcatgatttg ctcaacaatc attatttttt cccctttgtc agtgtcagtt aatacatata 3420 acgatgataa aaacggagcc tgtcaaattg ctcaattgat tcttggctga cgttttttcc 3480 aatgcgggcc atcagacagt ttgcagggtg ggagctgatc tccggctcat ccgttgcaaa 3540 ccagaccaat ccgcctgcat tcatcatctt ttccatgatc tttctgtatt cccacacatc 3600 ttttttcatc ccttttaaat cccaatgcca gtaatattct gtcgtcatca caatgtagtt 3660 ttccatttgt tcatccatca tgctgacggt caggagcgtc tttccgacag cgcatccccg 3720 atagtccggc gctacttcga tggctccgag ctcaatcaaa tcctccatat ttccttcaga 3780 ccatctttcg agcgggtcag gatataaata cgtgacatag ccgacaatgg ttcggccgtc 3840 tctggcaata ataatgcgcc cttcgggaag gcctgcaatg tcgactagcg cttcatgctg 3900 ctcgcgaggc gggcgaaatg cggttaaatc tttatgaaac tcataacctg ccaaatcctc 3960 tggagagaca ggcccttcta tcagtaaaga gccggttgct gttttgatgt ttgctgaatg 4020 gtatgtttta tgatgttcca ctaattcacc gtcccatcaa ttcttatcaa aaatacatgc 4080 cttctttaag aataccaaat tataaagcgt tttcaacatg gtttatattt taaaaattga 4140 gaagaatatg aatatatact ataataattg tgacaacttc agcaaagggg gatgtttggg 4200 catgaacttg aaagcgttac cagcaataga gggggatcat aacttaaaaa actatgaaga 4260 aacgtaccgg cattttgatt gggccgaggc agagaaacat ttctcttggc atgagacagg 4320 gaaactgaat gcggcgtatg aagcgattga ccgccatgcc gaatcgtttc gaaaaaacaa 4380 agtagcgctt tattataaag acgcaaaaag ggatgaaaaa tacacattta aagaaatgaa 4440 ggaagaatca aacagagccg ggaatgtgct gagacggtat ggaaatgtgg aaaaagggga 4500 ccgcgttttt atttttatgc cgagatcacc cgagctttat tttattatgc ttggcgcaat 4560 caaaattggc gccatcgccg ggccgctgtt cgaagcattt atggagggag cggtgaaaga 4620 ccggcttgaa aacagtgagg caaaggttgt tgtcacaacg cctgagctgc tggagagaat 4680 accggtagac aaactgcctc acttgcagca tgtcttcgta gtcgggggag aggctgagag 4740 cggcacgaat atcatcaatt atgatgaagc agcgaaacag gaaagcacaa gattggatat 4800 cgaatggatg gataaaaaag acggctttct gcttcactat acatcaggtt ccactggtac 4860 gccaaagggc gtgttgcatg tccatgaagc gatgattcag caatatcaaa caggaaagtg 4920 ggtccttgat ttaaaggaag aagacattta ttggtgcacg gctgatccag gctgggtgac 4980 aggtacggta tacggcattt ttgcaccgtg gctgaacgga gcgacaaatg tcatcgtcgg 5040 cggacgtttc agcccggaaa gctggtatgg aacgattgaa cagcttggcg tcaatgtctg 5100 gtacagcgcg ccgacagctt ttcggatgct gatgggagcg ggagatgaaa tggctgcgaa 5160 atatgatcta acttcactcc ggcatgtgct cagtgtcggt gagccgctaa atccggaagt 5220 catcagatgg ggacataaag tttttaacaa acgaatccat gatacctggt ggatgaccga 5280 aacgggcagt cagctcatct gcaactatcc ttgcatggat attaaaccgg gttcaatggg 5340 taagccgatt ccaggagtgg aggcagcgat cgttgacaat caaggcaacg agctaccgcc 5400 gtaccgaatg ggcaatctcg ccatcaaaaa gggctggcct tccatgatgc ataccatttg 5460 gaataaccct gaaaagtatg aatcgtattt catgccgggc ggctggtatg tgtctgggga 5520 ttctgcttac atggatgaag agggatactt ttggttccaa ggcagagttg atgacgtcat 5580 catgacctcc ggtgagcgcg tcggcccatt tgaagtggaa agcaagcttg tcgaacatcc 5640 ggctattgca gaagcaggcg ttatcggaaa gcctgacccg gtgcgtggag aaatcattaa 5700 agcctttatt gcactcaggg aaggatttga gccgtctgat aaactgaaag aagagatccg 5760 cctatttgta aagcagggtc ttgcagccca tgcggctccg cgtgagatcg aatttaaaga 5820 taagcttccg aaaaccagaa gcggaaagat catgaggcgc gtgctgaagg catgggagct 5880 taatctgccg gctggagatc tgtcaacaat ggaggattaa aaggcggcgt tgacacagga 5940 ttttatttat gttaaaaatg atatagcttc atatgaaaag gtaaagattg agacaagtag 6000 aatatcctta cgttccagag agctgatggc cggtgaaaat cagcacagac ggatatatcg 6060 aatacactca tgaaccgctt ttgcaaacaa agccggccag gctttcagta gtgaaagaac 6120 ggacctgatc cgttatcagg caaagtgata agacgaatgt ttgcattctc ttattagtag 6180 ggtggtaccg cgataatcaa tcgtcccttc gtgtaaacga aggggcgttt tttattttaa 6240 ttaaaaaagg agctttatct tatgactaac ttacttgaag acttatcctt ccgcggattg 6300 // |
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