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EBI DbfetchID L16808; SV 1; linear; genomic DNA; STD; PRO; 3033 BP. XX AC L16808; XX DT 04-JAN-1994 (Rel. 38, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 7) XX DE Bacillus subtilis orfK, orfL and orfM, complete cds's. XX KW . XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3033 RX DOI; 10.1016/0005-2728(94)90131-7 RX PUBMED; 8011666. RA Quirk P.G., Guffanti A.A., Clejan S., Cheng J., Krulwich T.A.; RT "Isolation of Tn917 insertional mutants of Bacillus subtilis that are RT resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone"; RL Biochim. Biophys. Acta 1186(1-2):27-34(1994). XX FH Key Location/Qualifiers FH FT source 1..3033 FT /organism="Bacillus subtilis" FT /isolate="MS11" FT /mol_type="genomic DNA" FT /tissue_lib="Tn917 insertional library no. 1" FT /db_xref="taxon:1423" FT RBS 51..57 FT /note="putative" FT CDS 69..1067 FT /codon_start=1 FT /transl_table=11 FT /label=orfK FT /note="Likely N-terminal signal sequence, followed by FT lipoamide anchoring site. No homologues in database.; FT putative" FT /db_xref="GOA:P40400" FT /db_xref="InterPro:IPR001638" FT /db_xref="UniProtKB/Swiss-Prot:P40400" FT /protein_id="AAA64348.1" FT /translation="MKKGLIVLVAVIFLLAGCGANGASGDHKQLKEIRIGIQQSLSPLL FT IAKEKGWFEDAFEKEGIKVKWVEFQSGPPQFEGLAADKLDFSQVGNSPVIAGQAAGIDF FT KEIGLSQDGLKANGILVNQNSGIQDVKGLKGKKIAVAKGSSGFDFLYKALDQVGLSAND FT VTIIQLQPDEAASAFENGSVDAWSIWEPYLSLETMKHGAKILVNGESTDLYSPGFTLVR FT TKFSEEHPDEVVRFLKVFNKAVVWQKEHLDEAADLYSDIKDLDKKVVENVLKNTEPLNE FT IISDDIVKAQQETADFQFRTKAIDKKIDVKDVVDNTFIKKALEEHSSGGDQ" FT RBS 1052..1057 FT /note="putative" FT CDS 1064..1894 FT /codon_start=1 FT /transl_table=11 FT /label=orfL FT /note="protein is hydrophobic, with homology to E. coli FT ProW; putative" FT /db_xref="GOA:P40401" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/Swiss-Prot:P40401" FT /protein_id="AAA64349.1" FT /translation="MMKAEAAGSLPKTNAEAVRKKPGRKRYGWMKGLLLPAVIIAIWQV FT IGGLGVVSATVLPTPVTIVLTFKELILSGELFGHLQISIYRAALGFLLGAGLGLMIGIL FT AGFSKRTELYLDPSLQMLRTVPHLAVTPLFILWFGFDEVSKILLIALGAFFPVYINTFN FT GIRGVDAKLFEVARVLEFKWHQQISKVILPAALPNILLGIRLSLGIAWLGLVVAELMGS FT SSGVGYMIMDARQFSQTNKVFAGIIIFAVVGKLTDSFVRLLERKLLKWRNSYEG" FT RBS 1904..1910 FT /note="putative" FT CDS 1917..3033 FT /partial FT /codon_start=1 FT /transl_table=11 FT /label=orfM FT /note="Gene disrupted by Tn917 insertion after base 3033. FT Translation product hydrophilic, no homologues in the FT databases.; putative" FT /db_xref="GOA:P40402" FT /db_xref="HSSP:1M41" FT /db_xref="InterPro:IPR016048" FT /db_xref="UniProtKB/Swiss-Prot:P40402" FT /protein_id="AAA64350.1" FT /translation="MEILWFIPTHGDARYLGSESDGRTADHLYFKQVAQAADRLGYTGV FT LLPTGRSCEDPWLTASALAGETKDLKFLVAVRPGLMQPSLAARMTSTLDRISDGRLLIN FT VVAGGDPYELAGDGLFISHDERYEATDEFLTVWRRLLQGETVSYEGKHIKVENSNLLFP FT PQQEPHPPIYFGGSSQAGIEAAAKHTDVYLTWGEPPEQVKEKIERVKKQAAKEGRSVRF FT GIRLHVIARETEQEAWEAAERLISHLDDDTIAKAQAALSRYDSSGQQRMAVLHQGDRTK FT LEISPNLWAGIGLVRGGAGTALVGDPQTIADRIAEYQALGIESFIFSGYPHLEEAYYFA FT ELVFPLLPFENDRTRKLQNKRGEAVGNTYFVK" XX SQ Sequence 3033 BP; 841 A; 610 C; 842 G; 740 T; 0 other; gaattcgaga aaacagaaga tttggagtac gcagaaggat cagggatata gaaagggcgg 60 aagcggaaat gaaaaaagga ctcattgtat tggttgccgt tatttttctg ctcgcaggct 120 gtggagcaaa tggagccagc ggtgaccaca aacaattgaa ggaaattcgt attggtattc 180 agcaaagctt aagccccctt ttgattgcaa aagaaaaagg ctggttcgaa gatgcatttg 240 aaaaagaagg catcaaagtg aaatgggtgg agtttcaaag cggtccgccc caatttgaag 300 gccttgcggc agacaaactt gatttttctc aagtcggtaa ttcgcctgtg atcgctggac 360 aggcggcagg cattgacttt aaagaaatcg gtttgtctca ggacggcttg aaagcaaacg 420 gcatccttgt caatcaaaac agcggcattc aagacgttaa gggcttaaag gggaagaaaa 480 ttgcagtcgc caaaggcagc agcgggtttg attttttgta taaagcgctt gatcaagtgg 540 ggttgtcagc taacgatgtg acaattattc agcttcagcc tgatgaagca gcttccgcat 600 ttgaaaacgg atctgttgat gcctggtcca tttgggagcc gtacttgtcc ttagaaacaa 660 tgaaacacgg agcaaaaatc cttgtgaacg gagaaagcac agatttatat tcaccgggtt 720 ttactttagt aagaacaaag ttttcagaag agcatccaga cgaagtggtc cgttttctga 780 aggtgtttaa taaagccgtt gtatggcaaa aggaacatct ggatgaagca gcggacttat 840 attctgacat taaagatctc gataaaaagg tcgtcgaaaa tgtgctgaaa aatactgagc 900 cacttaatga gatcatcagt gacgacattg tgaaagcaca gcaggaaaca gcagattttc 960 aatttcgaac aaaggcgata gataagaaga ttgatgtgaa ggatgttgtc gataacactt 1020 ttatcaaaaa agcattagag gagcattcct caggaggtga tcaatgatga aagcagaggc 1080 tgcgggctcg ctgcccaaaa cgaatgcgga agcggtgcga aaaaagcccg gaaggaagag 1140 atacggctgg atgaaggggc ttttactgcc cgctgtcatc attgccatct ggcaggtcat 1200 cggcgggctg ggggttgtgt ccgctacagt gctgcctaca cctgttacga ttgttctgac 1260 ctttaaggag cttattttgt ccggcgaact gttcggccat ctgcaaatca gcatttatcg 1320 ggcggcactt ggttttttgc ttggcgcggg gcttggattg atgatcggga ttttggccgg 1380 attttccaaa cggacggagc tgtatcttga tccgtcgttg caaatgctta gaaccgtacc 1440 tcatctggcc gtcacgccgc tgtttatttt atggttcgga tttgacgaag tatctaaaat 1500 tttgctgatt gcgctcggtg ccttctttcc tgtctatatc aatacgttta acggcatccg 1560 cggggttgac gcgaagctgt ttgaagtcgc ccgggtgctt gaatttaaat ggcatcagca 1620 gatatccaaa gtgatattgc cggcagccct gcccaatata ttgctaggca ttcgcttatc 1680 gctcggcatt gcatggctcg ggctcgttgt ggcagagctg atgggctcaa gctcgggagt 1740 cggctatatg atcatggatg cgagacagtt ttcacaaacg aataaagtct ttgcagggat 1800 tatcattttt gcagtggttg gaaagctgac ggattccttc gtcaggctgc ttgaacgaaa 1860 gctgttaaag tggagaaaca gctacgaagg ataaaagata taaaggggag atgaatatgg 1920 aaattctttg gtttataccg actcatggag acgcgagata tttaggatct gaaagtgatg 1980 gacggactgc ggatcatctt tactttaaac aagtcgcgca ggcggcagac aggctgggat 2040 atacaggagt tcttctgcca acgggaagat cgtgcgagga cccgtggctt acagcgtcgg 2100 cactggcagg agaaacaaag gacttaaagt ttttagtcgc ggtaagacct ggtttaatgc 2160 agccttctct ggcagccaga atgacatcca ccctagaccg catttcagac ggccggctgc 2220 tgatcaacgt tgtggcaggg ggcgatccgt atgagctggc aggcgatggc ctgtttatca 2280 gccatgatga gcgctacgag gcaacagacg aatttttaac cgtttggcgg agacttttgc 2340 agggagaaac ggtttcctat gaaggaaagc atataaaagt ggaaaacagc aatctgcttt 2400 tcccgccgca gcaagagccg catccgccca tctatttcgg tggttcgtca caggctggaa 2460 ttgaagctgc cgcaaagcat acagacgtgt acttgacatg gggtgagccg cctgaacaag 2520 taaaagaaaa gatagaaaga gtcaaaaagc aagcggcaaa agaagggaga tccgttcgat 2580 ttggcattag gctgcatgtc attgcccgtg aaactgaaca agaggcatgg gaagcggcag 2640 agcgcttgat cagccacttg gatgatgaca ccattgccaa agcgcaggca gctttgagcc 2700 gttacgactc ttcaggccag cagcggatgg ccgtccttca tcaaggcgat cggacgaagc 2760 ttgaaatcag cccgaatctg tgggcgggga tcggcctggt cagaggaggg gcaggaacag 2820 ctcttgtcgg tgatccgcag acgattgccg accggatagc ggagtaccag gcactcggca 2880 tagaatcgtt tatattctcc ggctatccgc atttagaaga agcgtattat tttgcagagc 2940 ttgtatttcc gcttttgccg tttgaaaatg atcggacgag aaagcttcag aacaaaaggg 3000 gagaagcggt aggaaacact tattttgtca aga 3033 // |
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