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EBI DbfetchID L14580; SV 1; linear; genomic DNA; STD; PRO; 6357 BP. XX AC L14580; XX DT 21-APR-1993 (Rel. 35, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 10) XX DE Bacillus subtilis glutamyl-tRNA transferase (gltX), serine DE acetyltransferase (cysE), and cysteinyl-tRNA synthetase (cysS) genes, DE complete cds's. XX KW cysE gene; cysS gene; cysteinyl-tRNA synthetase; gltX gene; KW glutamyl-tRNA synthetase; serine acetyltransferase; T-box; transfer RNA; KW tRNA synthetase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6357 RX PUBMED; 2120226. RA Breton R., Watson D., Yaguchi M., Lapointe J.; RT "Glutamyl-tRNA synthetases of Bacillus subtilis 168T and of Bacillus RT stearothermophilus. Cloning and sequencing of the gltX genes and comparison RT with other aminoacyl-tRNA synthetases"; RL J. Biol. Chem. 265(30):18248-18255(1990). XX RN [2] RX PUBMED; 1735721. RA Henkin T.M., Glass B.L., Grundy F.J.; RT "Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory RT sequence in multiple tRNA synthetase genes"; RL J. Bacteriol. 174(4):1299-1306(1992). XX RN [3] RP 1-6357 RX PUBMED; 7510287. RA Gagnon Y., Breton R., Putzer H., Pelchat M., Grunberg-Manago M., RA Lapointe J.; RT "Clustering and co-transcription of the Bacillus subtilis genes encoding RT the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and RT the first enzyme for cysteine biosynthesis"; RL J. Biol. Chem. 269(10):7473-7482(1994). XX FH Key Location/Qualifiers FH FT source 1..6357 FT /organism="Bacillus subtilis" FT /map="11-16 degrees" FT /strain="168T" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS <1..435 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /db_xref="InterPro:IPR002792" FT /db_xref="UniProtKB/Swiss-Prot:Q06754" FT /protein_id="AAA21793.1" FT /translation="DILNRIQKELDIEVEIYEGDFEDIQEVDSKLVKLAKLTSGVVVTN FT DFNLNKVCELQKVAVLNINDLANAVKPVVLPGEEMNVQVIKDGKEHNQGVAYLDDGTMI FT VVEEGRNYIGKHIDVLVTSVLQTAAGRMIFAKPKLLEKAL" FT CDS 448..1146 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /db_xref="GOA:Q06755" FT /db_xref="HSSP:1I52" FT /db_xref="InterPro:IPR018294" FT /db_xref="UniProtKB/Swiss-Prot:Q06755" FT /protein_id="AAA21794.1" FT /translation="MSYDVVIPAAGQGKRMKAGRNKLFIELKGDPVIIHTLRVFDSHRQ FT CDKIILVINEQDREHFQQLLSDYPFQTSIELVAGGDERQHSVYKGLKAVKQEKIVLVHD FT GARPFIKHEQIDELIAEAEQTGAAILAVPVKDTIKRVQDLQVSETIERSSLWAVQTPQA FT FRLSLLMKAHAEAERKGFLGTDDASLVEQMEGGSVRVVEGSYTNIKLTTPDDLTSAEAI FT MESESGNKHV" FT CDS 1139..1615 FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /db_xref="GOA:Q06756" FT /db_xref="HSSP:1JY8" FT /db_xref="InterPro:IPR003526" FT /db_xref="UniProtKB/Swiss-Prot:Q06756" FT /protein_id="AAA21795.1" FT /translation="MFRIGQGFDVHQLVEGRPLIIGGIEIPYEKGLLGHSDADVLLHTV FT ADACLGAVGEGDIGKHFPDTDPEFKDADSFKLLQHVWGIVKQKGYVLGNIDCTIIAQKP FT KMLPYIEDMRKRIAEGLEADVSQVNVKATTTEKLGFTGRAEGIAAQATVLIQKG" FT -35_signal 1622..1627 FT /gene="gltX" FT -10_signal 1643..1648 FT /gene="gltX" FT RBS 1688..1698 FT /gene="gltX" FT CDS 1706..3157 FT /codon_start=1 FT /transl_table=11 FT /gene="gltX" FT /product="glutamyl-tRNA synthetase" FT /db_xref="GOA:P22250" FT /db_xref="HSSP:1J09" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P22250" FT /protein_id="AAA21796.1" FT /translation="MGNEVRVRYAPSPTGHLHIGNARTALFNYLFARNQGGKFIIRVED FT TDKKRNIEGGEQSQLNYLKWLGIDWDESVDVGGEYGPYRQSERNDIYKVYYEELLEKGL FT AYKCYCTEEELEKEREEQIARGEMPRYSGKHRDLTQEEQEKFIAEGRKPSIRFRVPEGK FT VIAFNDIVKGEISFESDGIGDFVIVKKDGTPTYNFAVAIDDYLMKMTHVLRGEDHISNT FT PKQIMIYQAFGWDIPQFGHMTLIVNESRKKLSKRDESIIQFIEQYKELGYLPEALFNFI FT GLLGWSPVGEEELFTKEQFIEIFDVNRLSKSPALFDMHKLKWVNNQYVKKLDLDQVVEL FT TLPHLQKAGKVGTELSAEEQEWVRKLISLYHEQLSYGAEIVELTDLFFTDEIEYNQEAK FT AVLEEEQVPEVLSTFAAKLEELEEFTPDNIKASIKAVQKETGHKGKKLFMPIRVAVTGQ FT THGPELPQSIELIGKETAIQRLKNI" FT misc_feature 3354..3371 FT /standard_name="T-box" FT /note="conserved sequence for T-box" FT /citation=[2] FT terminator 3379..3415 FT CDS 3459..4112 FT /codon_start=1 FT /transl_table=11 FT /product="serine acetyltransferase" FT /db_xref="GOA:Q06750" FT /db_xref="InterPro:IPR005881" FT /db_xref="UniProtKB/Swiss-Prot:Q06750" FT /protein_id="AAA21797.1" FT /translation="MFFRMLKEDIDTVFDQDPAARSYFEVILTYSGLHAIWAHRIAHAL FT YKRKFYFLARLISQVSRFFTGIEIHPGATIGRRFFIDHGMGVVIGETCEIGNNVTVFQG FT VTLGGTGKEKGKRHPTIKDDALIATGAKVLGSITVGEGSKIGAGSVVLHDVPDFSTVVG FT IPGRVVVQNGKKVRRDLNHQDLPDPVADRFKSLEQQILELKAELEDRKERINQK" FT CDS 4109..5509 FT /codon_start=1 FT /transl_table=11 FT /product="cysteinyl-tRNA synthetase" FT /db_xref="GOA:Q06752" FT /db_xref="HSSP:1LI5" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:Q06752" FT /protein_id="AAA21798.1" FT /translation="MTITLYNTLTRQKETFVPLEEGKVKMYVCGPTVYNYIHIGNARPA FT IVYDTVRNYLEYKGYDVQYVSNFTDVDDKLIKAANELGEDVPTISERFIKAYFEDVGAL FT GCRKADLHPRVMENMDAIIEFVDQLVKKGYAYESEGDVYFKTRAFEGYGKLSQQSIDEL FT RSGARIRVGEKKEDALDFALWKAAKEGEISWDSPWGKGRPGWHIECSAMVKKYLGDQID FT IHAGGQDLTFPHHENEIAQSEALTGKTFAKYWLHNGYINIDNEKMSKSLGNFVLVHDII FT KQHDPQLLRFFMLSVHYRHPINYSEELLENTKSAFSRLKTAYSNLQHRLNSSTNLTEDD FT DQWLEKVEEHRKAFEEEMDDDFNTANAISVLFDLAKHANYYLQKDHTADHVITAFIEMF FT DRIVSVLGFSLGEQELLDQEIEDLIEKRNEARRNRDFALSDQIRDQLKSMNIILEDTAQ FT GTRWKRGE" FT CDS 5928..>6357 FT /codon_start=1 FT /transl_table=11 FT /note="ORFD1" FT /db_xref="GOA:Q06753" FT /db_xref="HSSP:1GZ0" FT /db_xref="InterPro:IPR013123" FT /db_xref="UniProtKB/Swiss-Prot:Q06753" FT /protein_id="AAA21799.1" FT /translation="MSQQHDYVIGKNAVIETLKSDRKLYKLWMAENTVKGQAQQVIELA FT KKQGITIQYVPRKKLDQMVTGQHQGVVAQVAAYEYAELDDLYKAAEEKNEQPFFLILDE FT LEDPHNLGSIMRTADAVGAHGIVIPKRRAVGLTTTVAKA" XX SQ Sequence 6357 BP; 1969 A; 1196 C; 1622 G; 1570 T; 0 other; gatatcctga accgcattca gaaagaatta gacattgaag ttgaaattta cgaaggcgac 60 tttgaagata ttcaggaggt tgacagcaag ctcgtcaaac ttgctaagct gacatccggg 120 gtcgttgtga cgaatgattt taatttaaat aaagtgtgcg agcttcagaa ggtagcagta 180 ttaaacatta atgaccttgc caatgcggtt aagcctgttg tgcttcctgg cgaggaaatg 240 aatgttcagg tcattaagga cgggaaagag cataatcagg gagttgccta cttggatgac 300 ggtacgatga ttgtcgttga agaaggacgg aattatatcg gcaagcatat tgatgtactc 360 gtcacaagtg tgctgcagac agcagcggga cgaatgattt ttgcgaagcc taagctgttg 420 gagaaggcgc tgtaaaggga gaagaacatg agttatgatg tggtgattcc tgcagccgga 480 cagggaaagc ggatgaaggc agggagaaat aaactgttca ttgagctgaa gggagacccg 540 gtgatcatac acacgttaag agtgtttgac agccaccggc agtgcgataa aatcattttg 600 gtgattaacg agcaggaccg ggagcacttt cagcaattgc tgtccgatta cccgtttcaa 660 acttcaattg agcttgttgc aggcggagat gagcgacagc acagtgtgta taaggggctg 720 aaagccgtaa agcaggaaaa gattgtcctt gtacatgacg gtgcccgtcc atttataaaa 780 catgaacaaa ttgacgaact gatcgcagag gcggaacaga caggagcggc catccttgct 840 gttccggtaa aagatacgat taaacgcgtt caagatttac aagtcagtga gacgattgaa 900 cgttcaagct tgtgggctgt ccaaacgcca caagcttttc gtctttcttt attgatgaag 960 gctcacgctg aggccgagcg caagggattt ttagggacgg atgacgccag cctcgttgaa 1020 cagatggagg gcggttcggt ccgtgttgta gaaggcagct atacaaatat taagctgacg 1080 acgccagacg atttgacgtc agctgaagct atcatggaat cagaaagtgg gaataaacat 1140 gtttagaatt ggacaaggat ttgatgtgca tcaattagtg gagggccgtc ctctcattat 1200 tggcggaatt gaaatcccgt acgaaaaagg gctgcttggc cattctgatg cagacgtatt 1260 gttgcatacc gtcgctgacg cctgcctggg agctgtgggt gaaggagaca taggcaagca 1320 ttttcctgac acagatcccg agttcaagga cgcagattct ttcaaattac ttcagcatgt 1380 ctggggaatc gtgaaacaga aggggtatgt ccttgggaac attgattgca ccatcatagc 1440 gcaaaagccg aagatgctgc cgtacataga agatatgaga aaaagaattg ctgaaggcct 1500 tgaggcagat gtttctcaag taaatgtaaa agcaacaacg acagaaaagc ttggatttac 1560 aggccgggcg gaaggaatag cggctcaggc gacagtactg atacaaaaag gctaacttgt 1620 tttgatgccg ggtctatttg gtggtagaat agattcatac atttttgcct gaggccatac 1680 atgacatgaa aggaagtatt tgaaaatggg aaacgaagta cgcgtccgtt atgcaccgag 1740 tccaaccgga catttgcata ttggaaatgc cagaacggcg ctttttaatt atttatttgc 1800 ccgcaatcaa ggcggtaagt ttatcattcg agttgaggat actgataaaa agcgcaatat 1860 tgagggcgga gaacaaagcc agctgaatta tctgaagtgg ctaggtattg actgggatga 1920 gagtgtggat gtcggaggag agtacggtcc ataccgtcag tcagagcgta acgatatcta 1980 taaagtgtac tatgaagagc ttcttgaaaa agggcttgct tataaatgtt actgtacgga 2040 agaagagctt gaaaaagagc gtgaagaaca gattgcccgc ggagaaatgc ctcgttattc 2100 cggaaaacac agagacctga ctcaggaaga acaggagaaa tttatcgccg aaggcagaaa 2160 accaagtatt cgtttccgtg tgccggaagg aaaagtcatc gccttcaacg acatcgtaaa 2220 aggcgaaatt tcttttgaat cagatggcat cggcgacttc gttattgtga aaaaggacgg 2280 aacgcctact tataacttcg cggtagctat tgatgactac ttaatgaaaa tgacacacgt 2340 gctgcgcggt gaggatcata tttctaacac accgaaacag attatgatct atcaagcatt 2400 cgggtgggat attcctcagt tcggacacat gacgctgatt gtaaacgaaa gccgtaaaaa 2460 gctcagcaaa cgtgatgaat ccattattca attcatcgag cagtacaaag agcttggcta 2520 cctgccagaa gcgctgttca actttatcgg cttgttaggc tggtcaccgg ttggagaaga 2580 agagcttttc acaaaagagc agtttattga aatttttgat gtaaatcgtt tatctaaatc 2640 accagctttg tttgatatgc ataagctaaa atgggttaac aaccaatatg tgaagaagct 2700 ggatcttgat caggttgttg aactgacgct tccgcatttg caaaaagccg gcaaagttgg 2760 cactgagctt tctgctgaag aacaagaatg ggttcgtaaa ctgatttccc tgtatcatga 2820 gcaattaagc tacggtgcgg aaattgttga gctgactgat ttgttcttta cggatgagat 2880 cgagtataat caagaagcga aagctgttct ggaagaagaa caggttcctg aagtgctcag 2940 cacattcgca gcgaagcttg aagagcttga ggagttcact ccggataata tcaaagcatc 3000 gatcaaagca gtgcagaaag aaactggcca taaagggaaa aaactgttta tgccgattcg 3060 tgttgctgta acagggcaaa ctcacggtcc ggaactgccg caatcaattg aattgatcgg 3120 taaagagact gcaattcagc gtttaaagaa tatctaaata aatgattgtt gacagggaag 3180 agtataaagc tttaggccat tacagaaagg atcttcattg gctgagagag atccatgagc 3240 cgggttttag aagtgcgccc ttaagacctt tgaagaacgt tttttgaaat cagtattcaa 3300 aggcggccgc cgccgttaca ggctgaagtt gggagagcag aagtctgctt ttcaaacaga 3360 gtggaaccgc gcggttaaag cgtctctgtc atgtttacat gcagagacgc tttttttatt 3420 gggtagagga aatcagatag agaaacgggg ggaagcatgt gttttttaga atgctcaaag 3480 aagacattga tactgtgttc gatcaagatc ccgcagcaag aagctatttt gaagtgattt 3540 taacttattc cggtttacat gctatatggg cgcatcggat tgcacatgct ttatataaac 3600 gaaaatttta tttccttgca cgccttatat ctcaagtaag ccgatttttt accgggatag 3660 aaatccaccc cggcgctaca atcgggagaa gatttttcat agaccacggc atgggggttg 3720 tcattgggga gacatgtgaa atcggcaata acgtaaccgt ttttcagggg gttaccctcg 3780 ggggaacggg aaaagaaaag ggaaaaaggc acccaacgat taaagatgac gcattgatag 3840 ccacaggtgc taaagtgctc ggttctatta cggtcggtga aggctcaaag attggcgctg 3900 gttcagtagt gctgcatgat gttcctgatt tttcaacagt tgtcggtatc cctggacggg 3960 tcgttgtaca aaatggcaag aaagtaagac gcgatttaaa ccatcaggat ttgcccgatc 4020 cagttgctga ccgctttaag tctttggaac agcagatttt agagctgaag gcagaacttg 4080 aagacagaaa agaaaggatc aatcaaaaat gacaatcaca ctttataata cattgactag 4140 acagaaggaa acattcgttc ctcttgaaga gggaaaagtg aaaatgtatg tatgcggacc 4200 cacggtttac aattacattc atatcgggaa cgcgcgtccg gcaatcgttt acgatacggt 4260 tcgaaactat ttagagtata aaggctatga tgtgcagtat gtctctaact tcacagacgt 4320 agacgataaa ttaattaaag cggcaaatga actcggtgag gatgtaccca ccatttcaga 4380 gcgttttatt aaagcatact ttgaagacgt aggtgcgctc ggctgccgaa aagccgacct 4440 tcatccgcga gtaatggaga acatggatgc cattatcgaa ttcgtagatc agctcgtgaa 4500 aaagggctac gcatatgaat cagaaggtga cgtatatttc aaaaccagag catttgaagg 4560 gtacggaaag ctttctcagc aatcaatcga tgaactaaga tcaggtgcac gcatccgggt 4620 cggcgagaaa aaagaagatg ctcttgattt cgcactgtgg aaagcggcaa aagaaggaga 4680 aatctcttgg gatagccctt gggggaaagg gcgtccgggc tggcacattg aatgctcagc 4740 aatggtgaaa aagtatctcg gtgaccagat tgatatccat gcgggcggac aggatttaac 4800 attccctcac catgaaaacg aaattgcgca atctgaggcg ctgacaggca aaacgtttgc 4860 gaagtactgg cttcataatg gttatatcaa tattgataat gaaaaaatgt caaaatcact 4920 aggcaacttt gtgcttgtgc atgacatcat taaacagcat gatccgcagc ttttgagatt 4980 ctttatgcta tctgttcatt atcgccatcc gattaactat tcagaagagc ttctggagaa 5040 tacgaaaagc gcgttcagcc gtttaaaaac agcgtacagc aatcttcagc accgtctgaa 5100 cagcagtacg aatttaaccg aagatgacga tcaatggctt gaaaaggttg aagaacaccg 5160 caaagcattc gaagaagaga tggacgatga ttttaatacg gcgaatgcca tttcagtctt 5220 gtttgactta gcgaaacacg ccaattatta tcttcagaaa gatcatacgg ctgatcatgt 5280 gattacggcg tttattgaga tgtttgaccg cattgtttct gtcctcggtt tttcgttggg 5340 tgagcaggaa cttctcgatc aagagattga agacttaatc gaaaagcgaa atgaagcgcg 5400 ccggaatcgc gattttgcat tgtcagacca gatccgcgac cagctgaaaa gcatgaatat 5460 cattcttgaa gatacggctc aaggcactcg ctggaaacgg ggagaataac agatgcttga 5520 atttgatacg ataaaagatt ctaagcagct taacggtctt gcgcttgctt atataggtga 5580 tgccattttt gaagtgtatg tcaggcatca cctgcttaag cagggcttta ccaaaccaaa 5640 tgatcttcat aagaaatcaa gccggattgt ttcagcaaag tcacaggctg agatcctatt 5700 ttttctgcag aatcaatcat tttttacgga agaagaggaa gcggtgctga aaagaggcag 5760 aaatgccaag tcagggacaa cacctaaaaa tacagatgtt cagacgtacc gctacagtac 5820 agcatttgaa gcgcttctgg gctacctttt tctagagaaa aaagaggaac gacttagtca 5880 gctcgtagcc gaagctatac aattcgggac gtcagggagg aaaacaaatg agtcagcaac 5940 atgattacgt catagggaaa aatgcagtga tcgagacgtt aaaatctgat cggaagctgt 6000 ataagctgtg gatggcggaa aacaccgtaa agggacaagc acagcaagtg attgagcttg 6060 ccaaaaagca gggaatcacg attcaatacg tcccgagaaa aaaactcgat caaatggtga 6120 caggacagca tcagggcgta gtggcacagg ttgcagcgta tgaatatgca gaactggacg 6180 atttatataa agcagccgaa gaaaaaaatg aacagccttt cttcctcatt ctggacgagc 6240 ttgaagaccc tcataatctt ggttccatta tgaggacagc agatgcggtc ggcgctcatg 6300 gcatcgtcat tccaaaacgg agagctgtcg ggctgacaac aacagtggca aaagctt 6357 // |
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