EBI Dbfetch

ID   L14564; SV 1; linear; unassigned DNA; STD; PRO; 2153 BP.
XX
AC   L14564;
XX
DT   21-APR-1993 (Rel. 35, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 7)
XX
DE   Neisseria gonorrhoeae mutant restriction endonuclease (dcrB) and cytosine
DE   methylase (dcmB) genes, complete cds's.
XX
KW   cytosine methylase; dcmB gene; dcmR gene; mutant; restriction endonuclease;
KW   restriction enzyme; restriction-modification system.
XX
OS   Neisseria gonorrhoeae
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
XX
RN   [1]
RX   DOI; 10.1007/BF00334379
RX   PUBMED; 2501649.
RA   Sullivan K.M., Saunders J.R.;
RT   "Nucleotide sequence and genetic organization of the NgoPII
RT   restriction-modification system of Neisseria gonorrhoeae";
RL   Mol. Gen. Genet. 216(2-3):380-387(1989).
XX
RN   [2]
RP   1-2153
RX   DOI; 10.1016/0378-1119(93)90509-2
RX   PUBMED; 8406039.
RA   Gunn J.S., Stein D.C.;
RT   "Natural variation of the NgoII restriction-modification system of
RT   Neisseria gonorrhoeae";
RL   Gene 132(1):15-20(1993).
XX
CC   NCBI gi: 293959
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2153
FT                   /organism="Neisseria gonorrhoeae"
FT                   /strain="F62"
FT                   /mol_type="unassigned DNA"
FT                   /db_xref="taxon:485"
FT   CDS             169..1006
FT                   /pseudo
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="dcrB"
FT                   /product="restriction endonuclease"
FT                   /note="mutant phenotype"
FT   variation       223..225
FT                   /gene="dcmB"
FT                   /gene_synonym="dcrB"
FT                   /replace="gaa"
FT                   /note="variation results in wild-type sequence X52661"
FT                   /citation=[1]
FT   variation       555..557
FT                   /gene="dcrB"
FT                   /note="back mutation to wild-type sequence X52661"
FT                   /citation=[1]
FT   CDS             978..2003
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="dcmB"
FT                   /product="cytosine methylase"
FT                   /note="NCBI gi: 293960"
FT                   /db_xref="GOA:P08455"
FT                   /db_xref="HSSP:1DCT"
FT                   /db_xref="InterPro:IPR018117"
FT                   /db_xref="UniProtKB/Swiss-Prot:P08455"
FT                   /protein_id="AAA17019.1"
FT                   /translation="MQNSSPTTYNPMKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTI
FT                   WATFKANHPKTHLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLF
FT                   FDYIRILKSKQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVA
FT                   QERKRVFYIGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDA
FT                   VNNNEYFTGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAA
FT                   GKETLYRRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLE
FT                   R"
XX
SQ   Sequence 2153 BP; 686 A; 528 C; 472 G; 467 T; 0 other;
     ggatgcccac gaacgccaac acctgcgcca aagcctgctt gaaacacggg aacacggctg        60
     acggccggac acgatgccgt ctgaaacgcc tgccgcttca gacggcatcg gtccgataaa       120
     cacctgacgg ttttgctata atctgccgca aaaccccaag gaaaaaccat gaacattatc       180
     gatgccatca tcaatttggc taacaaccct gttgtcgggg tcaactccca ttctcaaagc       240
     aacaatcgtg ccaatcaggc aggtgatgct ttggaagaat atgtaaaaga cttgttttcc       300
     ggcagtttca acctaaatga aacccaacgc atagcccgac acgcaaaagt attctcatat       360
     ttgggcaaca actccaaccc tcctgatgcc atgttgcgaa acggggatgc catcgaagtt       420
     aagaaaattg aaagtaaaga ttccgcactg gcgctgaata gcagccaccc aaaatcaaag       480
     ctctctgttg atgacagcat gcttacaaaa gcctgcaaag atgcggaaaa atgggaagag       540
     aaagacatta tctataattg tcggcgtcgt tgacaaaaag aaaaacctca aacacttggc       600
     aatggtttac ggaatcgact actgcgccga tgccgaatgc tatttgaaga taaaaaatca       660
     aattaaggaa ggaatcggca acatcggcgg catacagttt gcagaaacca aagagctggg       720
     cagggtcaac cgcattgatc cgctcaacat aacttatctg agagtacggg gtatgtgggg       780
     aatcgaaaat ccatggtttg tcttcaacta tatttaccaa cggaatatgg aaaaaagttt       840
     caatttcatg gccattatca acgaagacaa atggaacagt tttaacaata cagacaagct       900
     gcttgccata caggattcca aactggccat ttccgacatt aaaattaaaa atcccaacaa       960
     cccggcgcga ttaagaaatg caaaactcat cacctaccac ttataatcct atgaaaatca      1020
     ttagtttgtt tagcggttgc ggcggtttgg atttgggttt cgaaaaagcg ggatttgaaa      1080
     tccccgccgc caacgaatac gataaaacca tttgggcaac cttcaaggca aaccatccaa      1140
     agacccattt gatagaaggc gatatacgca agattaaaga agaagatttc cctgaagaaa      1200
     tcgacgggat tatcggcggc ccaccctgcc agtcttggtc tgaagcggga gctttgcgcg      1260
     gcatcgacga tgcgcgcgga cagttgtttt tcgactacat ccgtattttg aaaagcaaac      1320
     agccaaaatt ctttttagcg gaaaacgtca gcggaatgct ggcaaaccgc cacaacggag      1380
     ccgtacaaaa cctgctgaaa atgtttgacg gatgcggata cgacgtaacc ttgactatgg      1440
     ccaacgccaa agactacggt gtagcacagg aacgcaaaag ggtcttctac atcggtttcc      1500
     gtaaagactt ggaaataaaa ttttcttttc caaaaggttc gacggtcgaa gacaaagaca      1560
     agattacatt gaaagacgtt atttgggatt tgcaggacac agccgtacct tccgccccgc      1620
     aaaacaagac caaccccgac gcagtcaaca acaacgaata ttttaccggc agtttttccc      1680
     ctatttttat gagccggaac cgagttaaag cgtgggatga acagggtttt accgtccaag      1740
     cttcaggcag gcagtgccaa ctgcacccgc aagccccaaa gatggaaaag cacggggcaa      1800
     acgactaccg ttttgctgcc ggcaaagaaa cactatatcg gaggatgacg gtacgcgaag      1860
     ttgcaagaat ccaaggcttt cccgacaact tcaaattcat ctatcaaaat gtcaacgacg      1920
     catacaaaat gattggcaac gccgtccccg tcaaccttgc ctacgaaatt gcagcggcaa      1980
     ttaaaaaaac cctagaaagg tgaaacaccc ttcctacagg catctgcctt gtcgtcatgc      2040
     ccgaataacg gtactattcg cagttaacgg tctttatgcc gtctgaacgg cttggagcgc      2100
     gttcgaacgg ctgcccgcct accttatctt accgaaagaa caacaatatg atc             2153
//