EBI Dbfetch

ID   L12534; SV 1; linear; genomic DNA; STD; PRO; 1379 BP.
XX
AC   L12534;
XX
DT   16-MAR-1993 (Rel. 35, Created)
DT   05-JUL-1999 (Rel. 60, Last updated, Version 3)
XX
DE   Flavobacterium heparinum (clone pRSHEP.51) heparinase protein, complete
DE   cds.
XX
KW   heparinase.
XX
OS   Pedobacter heparinus
OC   Bacteria; Bacteroidetes; Sphingobacteria; Sphingobacteriales;
OC   Sphingobacteriaceae; Pedobacter.
XX
RN   [1]
RP   1-1379
RX   DOI; 10.1073/pnas.90.8.3660
RX   PUBMED; 8475114.
RA   Sasisekharan R., Bulmer M., Moremen K.W., Cooney C.L., Langer R.;
RT   "Cloning and expression of heparinase I gene from Flavobacterium
RT   heparinum";
RL   Proc. Natl. Acad. Sci. U.S.A. 90(8):3660-3664(1993).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1379
FT                   /organism="Pedobacter heparinus"
FT                   /mol_type="genomic DNA"
FT                   /tissue_lib="pUC18 genomic"
FT                   /db_xref="taxon:984"
FT   sig_peptide     173..235
FT   CDS             173..1327
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="heparinase"
FT                   /db_xref="GOA:Q05819"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q05819"
FT                   /protein_id="AAA24920.1"
FT                   /translation="MKKQILYLIVLQQLFLCSAYAQQKKSGNIPYRVNVQADSAKQKAI
FT                   IDNKWVAVGINKPYALQYDDKLRFNGKPSYRFELKAEDNSLEGYAAGETKGRTELSYSY
FT                   ATTNDFKKFPPSVYQNAQKLKTVYHYGKGICEQGSSRSYTFSVYIPSSFPDNATTIFAQ
FT                   WHGAPSRTLVATPEGEIKTLSIEEFLALYDRMIFKKNIAHDKVEKKDKDGKITYVAGKP
FT                   NGWKVEQGGYPTLAFGFSKGYFYIKANSDRQWLTDKADRNNANPENSEVMKPYSSEYKT
FT                   STIAYKMPFAQFPKDCWITFDVAIDWTKYGKEANTILKPGKLDVMMTYTKNKKPQKAHI
FT                   VNQQEILIGRNDDDGYYFKFGIYRVGNSTVPVTYNLSGYSETAR"
FT   mat_peptide     236..1324
FT                   /product="heparinase"
XX
SQ   Sequence 1379 BP; 465 A; 308 C; 281 G; 325 T; 0 other;
     ccttttggga gcaaaggcag aaccatctcc gaacaaaggc agaaccagcc tgtaaacaga        60
     cagcaattca tccgctttca accaaagtga aagcatttaa tacaatacca gaatgtcgca       120
     tttccctttc agcgtacttt ttgggtaaat aaccaataaa aactaaagac ggatgaaaaa       180
     acaaattcta tatctgattg tacttcagca actgttcctc tgttcggctt acgcccagca       240
     aaaaaaatcc ggtaacatcc cttaccgggt aaatgtgcag gccgacagtg ctaagcagaa       300
     ggcgattatt gacaacaaat gggtggcagt aggcatcaat aaaccttatg cattacaata       360
     tgacgataaa ctgcgcttta atggaaaacc atcctatcgc tttgagctta aagccgaaga       420
     caattcgctt gaaggttatg ctgcaggaga aacaaagggc cgtacagaat tgtcgtacag       480
     ctatgcaacc accaatgatt ttaagaaatt tcccccaagc gtataccaaa atgcgcaaaa       540
     gctaaaaacc gtttatcatt acggcaaagg gatttgtgaa caggggagct cccgcagcta       600
     taccttttca gtgtacatac cctcctcctt ccccgacaat gcgactacta tttttgccca       660
     atggcatggt gcacccagca gaacgcttgt agctacacca gagggagaaa ttaaaacact       720
     gagcatagaa gagtttttgg ccttatacga ccgcatgatc ttcaaaaaaa atatcgccca       780
     tgataaagtt gaaaaaaaag ataaggacgg aaaaattact tatgtagccg gaaagccaaa       840
     tggctggaag gtagaacaag gtggttatcc cacgctggcc tttggttttt ctaaagggta       900
     tttttacatc aaggcaaact ccgaccggca gtggcttacc gacaaagccg accgtaacaa       960
     tgccaatccc gagaatagtg aagtaatgaa gccctattcc tcggaataca aaacttcaac      1020
     cattgcctat aaaatgccct ttgcccagtt ccctaaagat tgctggatta cttttgatgt      1080
     cgccatagac tggacgaaat atggaaaaga ggccaataca attttgaaac ccggtaagct      1140
     ggatgtgatg atgacttata ccaagaataa gaaaccacaa aaagcgcata tcgtaaacca      1200
     gcaggaaatc ctgatcggac gtaacgatga cgatggctat tacttcaaat ttggaattta      1260
     cagggtcggt aacagcacgg tcccggttac ttataacctg agcgggtaca gcgaaactgc      1320
     cagatagcaa aagccctaag cgcatccgat agggcttttc ttatatttac aataaaatt       1379
//