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EBI DbfetchID L12019; SV 1; linear; genomic DNA; STD; PLN; 8062 BP. XX AC L12019; XX DT 06-MAR-1993 (Rel. 35, Created) DT 28-NOV-2007 (Rel. 93, Last updated, Version 7) XX DE Actinidia deliciosa var. deliciosa polygalacturonase gene, complete cds. XX KW polygalacturonase. XX OS Actinidia deliciosa var. deliciosa OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC asterids; Ericales; Actinidiaceae; Actinidia. XX RN [1] RP 1-8062 RX DOI; 10.1104/pp.103.2.669 RX PUBMED; 8029342. RA Atkinson R.G., Gardner R.C.; RT "A polygalacturonase gene from kiwifruit (Actinidia deliciosa)"; RL Plant Physiol. 103(2):669-670(1993). XX FH Key Location/Qualifiers FH FT source 1..8062 FT /organism="Actinidia deliciosa var. deliciosa" FT /cultivar="Hayward" FT /variety="deliciosa" FT /mol_type="genomic DNA" FT /sex="female" FT /dev_stage="young" FT /tissue_type="leaf and bud" FT /db_xref="taxon:284548" FT repeat_region 2933..3175 FT /rpt_type=TANDEM FT /rpt_unit_range=2933..3013 FT CDS join(3196..3513,4067..4195,4274..4441,4586..4606, FT 4963..5170,5981..6062,6228..6336,6675..6791,7376..7627) FT /codon_start=1 FT /product="polygalacturonase" FT /db_xref="GOA:P35336" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P35336" FT /protein_id="AAC14453.1" FT /translation="MALQRRFFQFVIITLLIPSFILGYTSAVHEDPPHDYHLEEYGYDF FT KAYPSYITTIGDNDFGSSMSHENGIFGLRKVDYGMDRVLDASKTVNVDDFGAKGDGRDD FT TKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQIYGTIEASDDRSDY FT RKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKALPCKDAPTALTFYKSKHV FT IVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGT FT GDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVR FT IKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIK FT GTCASNVAITFDCSKRFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSPHCQEE FT GGEEEEEAS" FT exon <3196..3513 FT /number=1 FT intron 3514..4066 FT /number=1 FT exon 4067..4195 FT /number=2 FT intron 4196..4273 FT /number=2 FT exon 4274..4441 FT /number=3 FT intron 4442..4585 FT /number=3 FT exon 4586..4606 FT /number=4 FT intron 4607..4962 FT /number=4 FT exon 4963..5170 FT /number=5 FT intron 5171..5980 FT /number=5 FT exon 5981..6062 FT /number=6 FT intron 6063..6227 FT /number=6 FT exon 6228..6336 FT /number=7 FT intron 6337..6674 FT /number=7 FT exon 6675..6791 FT /number=8 FT intron 6792..7375 FT /number=8 FT exon 7376..>7627 FT /number=9 FT polyA_signal 7816..7821 FT polyA_signal 7820..7825 FT polyA_signal 7878..7883 FT polyA_signal 7964..7969 XX SQ Sequence 8062 BP; 2696 A; 1276 C; 1296 G; 2794 T; 0 other; gaattcttgt aaaccacaaa tcttgatttc gatttgatgt ttgtttgcta ccattattgg 60 agcgtggaga ttggaataaa tgttaatgtt caaccaacac ccagaaaaag catgaaaata 120 tatcatagca taagagaaaa ttgcatgtgg atgagctcag ttttgtttgg ggtcatctaa 180 tcagacaatc attatgtggt tagggattaa gatgatgttt tagttgaacc ttagtaatat 240 atctctttgg ggtttcttaa cttagctgaa atgctaacat ccacaagaca tttccataat 300 caatcttagt cacaaacaaa ttattcggtc tcatatagca tggcatatgt tgcttcatga 360 tttgtcaccg agtacacgtg gcgtttgtcg atgggtggct gattttatga accaaaaatc 420 cttatcttaa tttgttagta gatcaaacac atatggttgc ttatatcagt tttaattagg 480 tcatgctacc acataatcat gagaaagaga gagagagaga ctaaagtctt catactcatc 540 atgcatggta cgaaggtaaa attgaaacct ccttggagat ttgagtcacc catgtcttta 600 ctccaagccc atccctacaa tcttctcaaa gttagattac tgcggttttt gtgatgcaat 660 catcaagtgg gcataacgta ggcaaaaagt atacgctttt gaatgagcac caactaatgc 720 caacgcaaca agtgtcacgt tctagcaata attcattatg caacgtactc atgcttacaa 780 gtatctgatt cttaaaatcc attacaagct caaaatttat acagaataat atgacagaat 840 gcatatatat atttaaacat aacattcaca aacatcgtca taatatgtta gaggactttt 900 tatgcataag tttttcgtaa gaataatatt ttccttattt aatgtcccaa ttgactgttc 960 taagttgttt tcttctcttg caggcaatca cctaagagaa gtgcaaacaa ttcataacat 1020 aggtaacttg aacatgattc tgtgacatca tgagcataca atttgttagc tcatgtactg 1080 cgaggtttgt atagatcatc accaaaacat agtgtataac cgccgatttt attacacgtg 1140 atcgattcgc catcaaggta acgatcaagt ctggtcattc atgaattaaa tacatttaac 1200 tatttacatc atgaaaaccc atgtggataa attcaaacac gcaaccttat gcatgtacct 1260 tgagcgcgcg catatagata tatatacata tacttaatgt tcaatcatat aattaactta 1320 tagtatacag cacttacgtg catgcatgag ctagctttta ctattaattt gcattggcat 1380 agaaaattag aaactcttat gggtcgtttg gctaccggat ttggaggggg ataacttttc 1440 ttcccggata atttttttcc ctcggcattt ggctagcgga tatggaggcg gataatttat 1500 gcctccgaag gtggatagag gcggataatt tttgggtggc ctaaacccta actaaaagtt 1560 atccggggat aagggggtgg gggataagga aaaaaatata taaagagaaa taaaatgtat 1620 aatttttttt atatagagag aaataaaaaa tatattttta atatctaaat attttatatt 1680 ttgtttctct ctatatattt tcttccttat cctccattct tatccaccca ttctaaaaag 1740 tatccaaaca aagttaaaag ttatcccctt ctaaaattta tccctctcta aaagttatcc 1800 ccctcctata tcctccagcc aaacgagccc ttaggtcttt actaaaacct tccaaaaatg 1860 aagtaattct actttatcaa gttgttaaat ttctttaaag atctatattg tgtgcagtaa 1920 tgttctgaat gattgtttaa ctctgttgta agtatactta gcatcgatac aataagattg 1980 tgtttggatt gaggatttgg agagagcaat aaaactgaaa gagaaaagtt aaaggatcta 2040 taacggtcct gaatggttga attatctaac ttttttattt atttatcttt ttttctcaca 2100 aaatccttaa ttcaaataac agtctaaaaa aattgcatgg tttagatttt ggggggataa 2160 cgagagtttt actgttacaa aagatttacc tagtgactta aagaaagttt tgctctatcc 2220 ttgcatgcat ttttttttta acaaaattct tcaatacaat aatgtagtgc tattgacata 2280 tttgaaaatt taaaccgaat taatgagcca cttatttcaa aatatatgac aaaaccatgg 2340 actacccaca tcttgttata ttgatttcta tacaaatttt ctgtctatat aacatatctc 2400 ataacatatt aagcaatgtg aaagtgttag ccattaactt atctaaaaat ttaagctatt 2460 aaaaagagga acgcataatt tttttattta ttattttctc aacaagtaag aattataaac 2520 attctaacta gttgttggat gctatctttt ttgtgcttat cttatttaat ttatttctaa 2580 gagaagaaag caagaaacta atcaattgtc ttaaaatatg ttcaaactct gaaaagaaac 2640 ctgaaagttt gcaaacactg aagtgttttg tttggggtct gtatttcata tctttggttt 2700 atctaggtgg ggaggttttg gctgtgcatg ctatctcaac cattcctcca ttttcccttg 2760 tttatgattt ggtgaataat aaaaattttc tttgcctatc aaaaataata tgttcatctc 2820 ctaaatgaaa tgaataaagg attagcttta tgtccatgct tatatgttta tatcttgtcc 2880 atgcctcaac aatcatctac gattaggact caattttaac ccctctctct ctccttctat 2940 aaatacacaa gactatctct aaacctttaa tcactctcca aaacaatctt gcccaccttt 3000 gggataatcc tttccttcta taaatacaca agactatctc taaaccttca atcattctcc 3060 aaaacaatct tgcacacctt tgggataatc ctttccttct acaaatacac aagactatct 3120 ctaaatcttt aatcattctc caaaacaatc ttgcacacct ttgggataat tctttctttg 3180 ttttagaaac aagctatggc acttcaaagg cgtttcttcc aatttgtcat cattaccctc 3240 ttaattccct ctttcatttt aggttacaca agtgctgttc atgaggaccc acctcatgat 3300 tatcaccttg aagaatatgg ttatgacttt aaggcatacc cttcttatat caccaccatt 3360 ggggacaatg actttgggag ctccatgagc catgaaaatg gaatctttgg gttgaggaaa 3420 gttgattatg gaatggatag ggttttggat gcatccaaaa ctgttaatgt ggatgatttt 3480 ggagctaagg gtgatggaag ggatgataca aaggtactca attagcacca ataatggttt 3540 taggggttta catttgttct ttttataatc actaatcatt tgcagatgta gaatatcaca 3600 gagtcatacc acgtgagact gaattattga gtcaatttga ttcaatggtt ggacccataa 3660 atatgcctcg atgacacaaa aaatcatttt tgctttttgt ttctatggtt agtaaacact 3720 aaaacctttt tttttcctaa tgaaattctc tttttctttt tcttttttat gagaatcatc 3780 acccaaataa tataccaata gtatttttaa aattattagt tatcctgatg agtttgattg 3840 gtctaaaatg atcaaataat aatctacagt cacgtcattt gtatgttttc tttactattt 3900 tttttttggt taattagaag ggtataaact gttggttttc tatgtgtggc catcattggc 3960 attaccaatt actttaatta aaacatttca aatttgttgg aaatgtcttt attgtttatt 4020 gtgttctgat aactgtaatt tgcttacctc atgaggtctt tattaggctt ttgagaaggc 4080 ttggaaggca gcttgttcat ctacaagtag tgctgttctc ttggtcccca aaaagaacta 4140 tcttgttagg ccaatatcat tctcaggccc atgcaaatct ggtctcacaa tgcaggtaag 4200 agagagagag agagagtttt cattacattt tcaagagatt tttttgacaa aaatattttg 4260 tatgaatttt cagatttatg gaacaattga agcatccgat gatcgatccg actacagaaa 4320 agatgggaga cactggcttg tatttgatag tgttcaaaat ctaagggttg aaggaggtgg 4380 aaccatcaat ggcaatggaa aaatatggtg gcaaaactca tgcaagacga ataaagctct 4440 tgtaagccat tcccatttca aattctttaa tttctcctat tatagtttta attttgtttg 4500 ctctttataa tttctttatc ggtatccaca tttttacaat cgacaattta ctaatcattt 4560 atacctcttt ctatctgttt tgtagccctg caaggacgct ccaacggtac gttaattaat 4620 tcatcttcta tgtgatatcg ttttgttttt tgtttttaat tttacatgaa actcaaaaaa 4680 ttgtttcgag aactggttgt gaattttttt ttctagaaag ttatcgagaa agaaatgatg 4740 tttttcaaaa tttcatgaaa tgtttttgag aaaaatgaaa attgtataaa aattgacttt 4800 ccgttggttt tatttctgaa accacgaaaa acgaacaaaa caaaaacgaa aaacgaaatt 4860 taggtaacga aaaataatcc taacctagtt tgagaaatat aagggaaaaa agtatattct 4920 atcttaaaat taatcatgta aacttcattt tattataagc aggccctgac attttataag 4980 agcaagcatg tgatcgtgaa gaacctcaag attgaaaacg ctcaacaaat tcatgtttct 5040 tttgataatt gtgtgaatgt tcaagcatcc aatctcatgg tgacggcacc ggaaaatagc 5100 cctaacaccg atggaattca cgtcaccggt acccaaaaca tccatatttc aagctgcgtc 5160 atagggacag gtatgcctaa taatgaaata tacaagtttt ggaaacaact ttgaagccag 5220 ctttaattta tttgttttta atgaaattct ttttaatttt tgtcggtttt ctttcaagta 5280 tataacaatt tttaattaaa agatcaaaat ttaaaaagtt tgcgaaagat aaaaaaatca 5340 aattttctaa aaaacttgga acaataacac gaaggaacat gtttttttca tttgggtgac 5400 aagagaccac atcattctat acaggtattt tatacaataa ttatgtttta cacaatacta 5460 gtaattactc atgtccataa acactatgca aaaaaaaatt attaggtgta ctcgttagag 5520 ttttacaact taagaaacag ataagatata acgtcagata agatatacat tgcacgcata 5580 tacgaagagt tacattatta caaaatggag aataaattaa aaaatataat aatttaaaaa 5640 tgaggaaaga aaatgtgttt taattttttt ttgatgtggg ttcaactaaa aaaatgatcc 5700 aataaataaa aaatatgtcc agctttagag attctttggt cattggaaca ctgcctatgt 5760 gggtctcaca ttttttagaa gaatgaagtg atattttttt gtaggaaaaa ataatctcag 5820 gtgaaatctc agccgttgga ttaatttaaa tgaatttcaa ccgcagatag taaaaaatag 5880 ttttaagatt caaaattagt tgtattttag attttagatt ttactcaaaa tattaactgt 5940 tcgtaaatcg taatctatat tttcctttta tatattgaag gtgatgattg tatttcaatt 6000 gtaaacgggt ctcggaaggt gcgggtcaat gatataacct gtggaccagg tcatggaatc 6060 aggtctcaag ctcaaacctc tagttaattt taccaattat tcaccaattt caactaaact 6120 aatagcattt tttttcgata tactatattt tgttgtaatt atattggtaa ttggttttga 6180 aattttctac taattttttt atttttatct tttttgttga acttcagtat tggaagcttg 6240 ggatatggga attcagaggc tcatgtatca gatgtggtgg tgaatggagc taagctttgt 6300 ggaactacaa atggagtcag gattaagaca tggcaggtaa atttaaattt atttatttat 6360 tttattgaac tatacttgtt taatttgttt aaaattatca ccacagaata taatatttcc 6420 taagttttaa aaagttttag tgggttcaaa tatacttgtc tagattcagg gcggcactag 6480 caagggacta gcaggggcta tagctcaccc tcactttcaa aaaattataa aatttatttt 6540 aggtttttaa attttttaca ctttaccctc tctgaaatat atttaaaaaa aatcaaaatt 6600 tatatttaat attttaactc ccgacctaaa atcatgaatc cgcttctgtt tatgaatatt 6660 accatgctgt gcagggggga tctggaagtg caagcaacat caaatttcag aatgtggaaa 6720 tgcataatgt ggaaaacccc atcattatag accaaaatta ctgtgaccaa gacaaacctt 6780 gtcaagaaca ggtaaggaaa caactctaac ctcatcctct ccacaggcaa taaaataact 6840 taataaagaa aaaaaatgtt aatattcgag tgacaatgtc attttcataa ttgaaattta 6900 atcttgatag attttcattt taaggtaata gttcaataat tcagccacat tgttctgtat 6960 taatttttta tgcacggtgt aatttgccca atgtgggtcc accctgagct aaataaataa 7020 tattctatga tatgtttatc ttttaaggcc tttcaagatg attaataata taaaaaaatc 7080 aggttaataa ataagacatg gcagatgtca atggactgtc aacatactaa atgttcattt 7140 cttttaagaa aaatgtgttt cgactaggta aatatcaaca tccgattaat ctaactttta 7200 tatagttaaa cggatcaaat catttgagtg aaacccgtga agactcacaa ttgtaaatta 7260 tattgtgcaa tccaaattag ggacgagaga gaatcaagta acttttttat aagataaaag 7320 aataaaatca actctttata aatagatgat acaaattttc tgcttggttt ttcagagctc 7380 ggctgttcaa gtgaaaaatg ttgtctacca gaacatcaaa ggcacatgtg cctccaatgt 7440 cgccataaca tttgattgca gcaagaggtt cccatgccag gggattgtgt tggaagatgt 7500 tgatctagag atagaaggag gggcagcagc aaaagctttg tgcaacaatg tcgagctcag 7560 tgaaacagga gttgtttcac cacattgcca agaagaagga ggagaagaag aagaagaagc 7620 tagctaggaa aaatacaaat gttattgaca ttagaatttt taggtggaat aatattattg 7680 atatacaagt gatgccgggg tggatccagc aagtccctaa cagaggttat aacctttcct 7740 cattttaaaa aaattacaaa atttacttta aattttaaat ttttttatat tttagcctct 7800 tgaaatatat taaaaataaa ataaaatttt atatttaaga ttttagccct tgacttaaaa 7860 tcctgatccg tgatgcaata aaggtgagaa tttcttgtaa atggcacgca attggacttt 7920 tggtgctcac aaattcggca tctagtctca tatctgggta cctaataaaa gttctcattt 7980 tacaccttcc aatttataca aggattccaa tttcgtccct catcttctaa tttaaacaca 8040 ttaaaccata atgtttgaat tc 8062 // |
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