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EBI DbfetchID L08489; SV 1; linear; genomic DNA; STD; INV; 8399 BP. XX AC L08489; XX DT 12-MAY-1993 (Rel. 35, Created) DT 02-OCT-2007 (Rel. 93, Last updated, Version 10) XX DE Toxoplasma gondii dihydrofolate reductase-thymidylate synthase, complete DE cds. XX KW . XX OS Toxoplasma gondii OC Eukaryota; Alveolata; Apicomplexa; Coccidia; Eucoccidiorida; Eimeriorina; OC Sarcocystidae; Toxoplasma. XX RN [1] RP 1-8399 RX PUBMED; 8454599. RA Roos D.S.; RT "Primary structure of the dihydrofolate reductase-thymidylate synthase gene RT from Toxoplasma gondii"; RL J. Biol. Chem. 268(9):6269-6280(1993). XX RN [2] RX DOI; 10.1016/0166-6851(94)90060-4 RX PUBMED; 8008022. RA Donald R.G., Roos D.S.; RT "Homologous recombination and gene replacement at the dihydrofolate RT reductase-thymidylate synthase locus in Toxoplasma gondii"; RL Mol. Biochem. Parasitol. 63(2):243-253(1994). XX RN [3] RP 1-8399 RA Roos D.S.; RT ; RL Submitted (11-MAY-1993) to the EMBL/GenBank/DDBJ databases. RL D.S. Roos, Department of Biology, University of Pennsylvania, Philadelphia, RL PA 19104-6018, USA XX CC On Jun 3, 1994 this sequence version replaced gi:295356. XX FH Key Location/Qualifiers FH FT source 1..8399 FT /organism="Toxoplasma gondii" FT /strain="RH" FT /mol_type="genomic DNA" FT /dev_stage="tachyzoite" FT /tissue_lib="Supercos" FT /db_xref="taxon:5811" FT GC_signal 1009..1021 FT /note="putative" FT /citation=[2] FT GC_signal 1012..1024 FT /note="putative" FT /citation=[2] FT mRNA join(1027..1712,2203..2339,2805..3136,3546..3795, FT 4290..4383,4763..4835,5239..5380,5781..5873,6500..6810, FT 7342..8399) FT /product="dihydrofolate reductase-thymidylate synthase" FT /citation=[2] FT exon 1027..1712 FT /number=1 FT /note="alternative transcription initiation sites mapped at FT nt 1027 and 1055 by primer extension and RNase protection" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT 5'UTR 1027..1395 FT GC_signal 1029..1041 FT /note="putative" FT /citation=[2] FT GC_signal 1031..1043 FT /note="putative" FT /citation=[2] FT exon 1055..1712 FT /number=2 FT /note="alternative transcription initiation sites mapped at FT nt 1027 and 1055 by primer extension and RNase protection" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT CDS join(1396..1712,2203..2339,2805..3136,3546..3795, FT 4290..4383,4763..4835,5239..5380,5781..5873,6500..6810, FT 7342..7425) FT /codon_start=1 FT /product="dihydrofolate reductase-thymidylate synthase" FT /EC_number="1.5.1.3" FT /EC_number="2.1.1.45" FT /note="dihydrofolate reductase; bifunctional protein; the FT 251 amino acid DHFR domain is separated from the 289 amino FT acidTS domain by a junctional domain of 70 amino acids" FT /db_xref="GOA:Q07422" FT /db_xref="InterPro:IPR000398" FT /db_xref="InterPro:IPR001796" FT /db_xref="InterPro:IPR012262" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:Q07422" FT /protein_id="AAB00163.1" FT /translation="MQKPVCLVVAMTPKRGIGINNGLPWPHLTTDFKHFSRVTKTTPEE FT ASRLNGWLPRKFAKTGDSGLPSPSVGKRFNAVVMGRKTWESMPRKFRPLVDRLNIVVSS FT SLKEEDIAAEKPQAEGQQRVRVCASLPAALSLLEEEYKDSVDQIFVVGGAGLYEAALSL FT GVASHLYITRVAREFPCDVFFPAFPGDDILSNKSTAAQAAAPAESVFVPFCPELGREKD FT NEATYRPIFISKTFSDNGVPYDFVVLEKRRKTDDAATAEPSNAMSSLTSTRETTPVHGL FT QAPSSAAAIAPVLAWMDEEDRKKREQKELIRAVPHVHFRGHEEFQYLDLIADIINNGRT FT MDDRTGVGVISKFGCTMRYSLDQAFPLLTTKRVFWKGVLEELLWFIRGDTNANHLSEKG FT VKIWDKNVTREFLDSRNLPHREVGDIGPGYGFQWRHFGAAYKDMHTDYTGQGVDQLKNV FT IQMLRTNPTDRRMLMTAWNPAALDEMALPPCHLLCQFYVNDQKELSCIMYQRSCDVGLG FT VPFNIASYSLLTLMVAHVCNLKPKEFIHFMGNTHVYTNHVEALKEQLRREPRPFPIVNI FT LNKERIKEIDDFTAEDFEVVGYVPHGRIQMEMAV" FT exon 2203..2339 FT /number=2 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 2805..3136 FT /number=3 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 3546..3795 FT /number=4 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 4290..4383 FT /number=5 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 4763..4835 FT /number=6 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 5239..5380 FT /number=7 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 5781..5873 FT /number=8 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 6500..6810 FT /number=9 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..8008 FT /number=20 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7931 FT /number=19 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7914 FT /number=18 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7908 FT /number=17 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7900 FT /number=16 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7868 FT /number=15 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7765 FT /number=14 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7755 FT /number=13 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7753 FT /number=12 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7745 FT /number=11 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT exon 7342..7678 FT /number=10 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT 3'UTR 7426..8399 FT polyA_site 7678 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7745 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7753 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7755 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7765 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7816 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7868 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7900 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 7901..7950 FT /function="possible association with polyadenylation" FT /note="degenerate repeat; strong direct repeat (32/50 FT nucleotides; 12 nucleotides exact repeat) with bases FT 7973-8022" FT polyA_site 7908 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7914 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT polyA_site 7931 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT repeat_region 7973..8022 FT /function="possible association with polyadenylation" FT /rpt_type=DIRECT FT /note="degenerate repeat; strong direct repeat (32/50 FT nucleotides; 12 nucleotides exact repeat) with bases FT 7901-7950" FT polyA_site 8008 FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 8399 BP; 1775 A; 2255 C; 2130 G; 2239 T; 0 other; aagcttcgcc aggctgtaaa tcccgtgagt cgtcctcaca aatcatcaag caggtgtcct 60 cagggagact gcctgactga gttatgctaa ttcctttcta ctttggcgtg gtcacgtgta 120 accatatccg aatcatttct ctagccctac gaacaggtaa gagcgctagg gatgtccgtg 180 gagtagtgtg cttactcgat aatattcagt tgggactacc agcgaggcgc tcgctttgct 240 cacgcaatgc ctgagacagt tgcagaatga atggtaaccg acaaacgcgt tcatatgcgt 300 tttcaaactt agtagacgcg tactgtctga aactggcggt cacaggcacc agataacgcc 360 cttggcatcg gcatgtctcg tacagaggtc cgtatgtagt gccacgactt ctaaatccgg 420 cgacaggctg gtcttttgtc ttaccacgta ttagcccgcg tgcgatttct cggagcgcac 480 ctgttcaaca ctagaaaacg gagtttcctg atcgagaagc caccaccttt ccagaagttg 540 aacgctagca tgtcattcga ttttcacccc ccgcgtagtt cctgtgtgtc attcgttgtc 600 gagacaactc tgtcccgccc cggtgctgtt ccatatgcgt gactttcccg caattttttc 660 agactttcag gaaagacagg ctccggaacg atctcgtcca tgactggtaa atccacgaca 720 ccgcaatggc ccccagcacc tctatctctc gtgccagggg actaacgttg tatgcgtctg 780 cgtcttgtct ttttgcattc gctttccaaa aaagagagcc atccgttccc ccgcacattc 840 aacgccgcga gtgcggtttt tgtctttttt gagtggtagg acgcttttca tgcgcgaact 900 acgtggacat taagttccat tctctttttc gacagcacga aaccttgcat tcaaacccgc 960 ccgcggaaga tccgatcttg ctgctgttcg cagtcccagt agcgtcctgt cggccgcgcc 1020 gtctctgttg gtgggcagcc gctacacctg ttatctgact gccgtgcgcg aaaatgacgc 1080 catttttggg aaaatcgggg aacttcattc tttaaaagta tgcggaggtt tcctttttct 1140 tctgttcgtt tctttttctc gggtttgata accgtgttcg atgtaagcac tttccgtctc 1200 tcctccgtgc tttgttcgac atcgagacca ggtgtgcaga tccttcgctt gtcgatccgg 1260 agacgcgtgt ctcgtagaac cttttcattt taccacacgg cagtgcggag cactgctctg 1320 agtgcagcag ggacgggtga agtttcgctt tagtagtgcg tttctgctct acggggcgtt 1380 gtcgtgtctg ggaagatgca gaaaccggtg tgtctggtcg tcgcgatgac ccccaagagg 1440 ggcatcggca tcaacaacgg cctcccgtgg ccccacttga ccacagattt caaacacttt 1500 tctcgtgtga caaaaacgac gcccgaagaa gccagtcgcc tgaacgggtg gcttcccagg 1560 aaatttgcaa agacgggcga ctctggactt ccctctccat cagtcggcaa gagattcaac 1620 gccgttgtca tgggacggaa aacctgggaa agcatgcctc gaaagtttag acccctcgtg 1680 gacagattga acatcgtcgt ttcctcttcc ctgtgagcac acacagtagt cgccacacgc 1740 tgtttgagac gtgtcaatct ccaagagtgt ggacgctgtt ccacgtcttc aaatgtttcc 1800 caacatccgt cgtctagtag acacaccaac aaaaagcaca cggcgaatct gctcatcgga 1860 gggaggagcc ggggggcaca caactatcct caactctcga acgaacatat ccggggccgc 1920 gaagacgtcc agtctctcaa atccaacccg gaacgcaaac atttctgcat caagtcacga 1980 ttgcgccggt acctccatgt gtaagcagtt ccatgaaacc tccgatatta cacacgactg 2040 tggatatgaa ttatatgcag atgcatatat actgagacgc cgatgcaact ataggtttcc 2100 tggccctcca tggatatttc agaccttcct ctcacatttg gtttgcccgt acacctccgt 2160 tacgcttttt ttctggcttt cttcttcgtc tctgtttatc agcaaagaag aagacattgc 2220 ggcggagaag cctcaagctg aaggccagca gcgcgtccga gtctgtgctt cactcccagc 2280 agctctcagc cttctggagg aagagtacaa ggattctgtc gaccagattt ttgtcgtggg 2340 tatgttgtcc taaactcctt ggaactccat tcttggtcag aaacgtactg aaactgtata 2400 catgtatata cagatgtatg gataatatct agagaagata cagggaagac tggcaaggat 2460 gaaaagacat gcagctttaa cgaagcagag ggcattggcg agagggacgc ccgttatgct 2520 gtgtgatgtg gctgtgaatc ttacctcgcc gtttgacttg ctgcagcgct ttgtccactt 2580 gaacgtgact tcttgtttct accttcccca acgccttcta ttcccttcac tgcgaaagcg 2640 cgctcagtgg gccgtcaccg aacacccttg gttctttcgt tcagctgttg tcctctttct 2700 cgcgttgctt cctgtggcgt cgtggctcgg cttctctctc tttcctgttg gtgcgtccag 2760 actatgtcgc ctgtttcccc acccttctcg gcttgtgctt tcaggaggag cgggactgta 2820 cgaggcagcg ctgtctctgg gcgttgcctc tcacctgtac atcacgcgtg tagcccgcga 2880 gtttccgtgc gacgttttct tccctgcgtt ccccggagat gacattcttt caaacaaatc 2940 aactgctgcg caggctgcag ctcctgccga gtctgtgttc gttccctttt gtccggagct 3000 cggaagagag aaggacaatg aagcgacgta tcgacccatc ttcatttcca agaccttctc 3060 agacaacggg gtaccctacg actttgtggt tctcgagaag agaaggaaga ctgacgacgc 3120 agccactgcg gaaccggtaa gaggcaaccg aagcgcgtag ataagaaaaa caacaaagag 3180 aaggtgaaac acgaagagaa gggaaaatgc ggagaaaccg tggatttaca aagatatcaa 3240 gagcaatgct ttgtggagat tttttttaat tcagtagaga cacccgccgt gcgaggtgtg 3300 tagaaataac tgcgaccctg gagacagaga tgccgcgagt acaccacttg tcgtttttcc 3360 tcctatgttc atgacgggtg ctgaacgtct atcgtactta attggaggag tcgtctccga 3420 agcagctttg gctggccatc cgtgtgtttg ccttgttcct gaaaagccag aaggcgctcc 3480 acagtgaggc gatatacagg gacgcctacc ggagccccgt tttctgcctt tgtcgactct 3540 tgcagagcaa cgcaatgagc tccttgacgt ccacgaggga gacaactccc gtgcacgggt 3600 tgcaggctcc ttcttcggcc gcagccattg ccccggtgtt ggcgtggatg gacgaagaag 3660 accggaaaaa acgcgagcaa aaggaactga ttcgggccgt tccgcatgtt cactttagag 3720 gccatgaaga attccagtac cttgatctca ttgccgacat tattaacaat ggaaggacaa 3780 tggatgaccg aacgggtaac ggcgactgcg agaaaaagcc acaccgtttt ctcctgtgat 3840 tctgtccgca agccctcttt tgcttcatcc accctttgct attctccgcc gccttccttt 3900 tctgctccat gttcaattcg ttcgcttctt cagtctttcc atcttcccct gttacctctg 3960 tcattcgttt tcttgcctct atttaactgt gttctactca cagtctgcat tccgcgatag 4020 acgagcttcc acgtcttgcg tctcgacaag caactgtcat ttgtacgcgc ctccctccac 4080 cgtgaatcgg attgtcggtt cgccggttcc tgggtcagaa aaggcctgcg ccagtattct 4140 gaataatacc cttcgccatt gtaaagaggc gaaggaacaa agagatattt cggcgcatct 4200 tttgtgcggc gcgtttcctc gtgcttcaca ccgatgccct tctgtgcatg tcttctgctc 4260 ctcgtccttc tctctttttc cctgtttagg cgttggtgtc atctccaaat tcggctgcac 4320 tatgcgctac tcgctggatc aggcctttcc acttctcacc acaaagcgtg tgttctggaa 4380 agggtaaggg cgtcttcagt gaatgcatat atttgacttc agacattctt aactgtttga 4440 caaccaacgt acaaatttgt ttgtccgtgt gcgtgttcga catgtcaagt atgtgaagag 4500 tcgctactgt agactaacgc acgaaccaga tttgtttatc tgcatgcgct gtgcacccgt 4560 ttctgagtgt ctggagtttc cgcaaccttc ctttgaattt ctgggttcgt ttttttatgc 4620 gcgcactggt ttgcatgtgg cctgagagag cacagatcga aggtggggtg atgtggcgtc 4680 gctgcagaga aactccggcg aaggcgacag ataaaggaga gtggaaatca ttgaacagtg 4740 tcggtcgtct gttgtttcgc agggtcctcg aagagttgct gtggttcatt cgcggcgaca 4800 cgaacgcaaa ccatctttct gagaagggcg tgaaggcaag tctacgttgt acctcttgtc 4860 tctgccgaag ctcagatgtc tccacggcgt tggtttcttt tcgtttttgc tttcgtggca 4920 ttaccatcga gtcaccactc atagttgcgt gtgtctacat gttttctaga acgtccgttg 4980 tgttgcctcg tggcgaccgg cgcgagtgta tgtaccctgc gctgtcagaa gttgatcctt 5040 acgctgctaa tgggatcgtt tagaccttcc catagcactg actttcttct tcgcatgttc 5100 cactctgtgc gttcgtgtcc gtggcgtcgt ctgctctgtg aaaacgcgtc tcgatgcgca 5160 accgcacaaa gtaggggcgt acgtcagcag attctcatgc ctgttgatcg ccgtggtgtt 5220 ggtgcctgct cgtcttagat ctgggacaag aatgtgacac gcgagttcct cgattcgcgc 5280 aatctccccc accgagaggt cggagacatc ggcccgggct acggcttcca gtggagacac 5340 ttcggcgcgg catacaaaga catgcacaca gactacacag gtatatgccg gcaatgtgtg 5400 cagggaaggg gcacaagcgg aagaactctg gcgcaacagc acctggctga atcgtggttt 5460 gcctcaggtg cagatccggc cattttaaaa cgcaagttcc atcagacgcg gacaggaaat 5520 gacataacag acatatacat gtacgacgca gtgctccctc tcatcctgag acggatgtca 5580 aggtggacga aagccggtta tggcctcatc tggtgatgta gactcgccca tcccgtgatc 5640 cctccaggcg tcggtgtttg tatgctgttt tctggaacct tttacacctg cgttcacaca 5700 tgtctgcgca gatgcacagc gtcaggggcc tggagaagaa agacgtccag caattcgttt 5760 ctcacatgtg ttctttgcag ggcagggcgt cgaccagctg aagaatgtga tccagatgct 5820 gagaacgaat ccaacagatc gtcgcatgct catgactgcc tggaatcctg caggtgagcc 5880 ttgcgtgtcg ttcatctgtc tattgtctca ggtgcccatg catgcgtttt aacctcatgt 5940 tttctctgca tttgtgccgt ctctgcgtct tcacatggga aaggcacaag agcagccgtg 6000 gttgagtaca aacgacctca gtctacttcc attttgcagc tctcgcaaac gatgtcgcgt 6060 gtttgtaccc gcaacgcctc tctgtcttgc atccattttg cgtctttctt ggtgtctgta 6120 tgcctagtag tctgtcagtt cccgcgcgca ggctgcaccc cgcctttcat tgctcgcagc 6180 gtttgccccc ggttagtact cgtgtgtgtt ccctggatct ctctgcacat ggccattccg 6240 ttgttttgcc attccgccgc cgttgcttcg tccgcccccg ctttctcagc gcctttcttc 6300 cctctcgtgc cttgcctacc taccagcgtc ttccctattt tatcgccgag gtgctcgcgt 6360 tttctgcgtt ggctgcgctc gatcgcagtt cgcgtcacgt cttcgtcgaa tctgggactt 6420 cgagccacgg cgatggtggc gcctccgcgt gttttctctc accgtgcaca tttctccacg 6480 ctgttgcttt tgcgttcagc gctggacgaa atggcgctgc cgccttgtca cttgttgtgc 6540 cagttctacg tgaacgacca gaaggagctg tcgtgcatca tgtatcagcg gtcgtgcgat 6600 gtcggcctcg gcgtcccctt caacatcgct tcctattcgc ttttgacgct catggttgca 6660 cacgtctgca acctaaaacc taaggagttc attcacttca tggggaacac gcatgtctac 6720 acgaaccatg tcgaggcttt aaaagagcag ctgcggagag aaccgagacc gttccccatt 6780 gtgaacatcc tcaacaagga acgcatcaag gtgagcacaa gttgcaaagg aaaaacgaca 6840 gtcgatgcca gcagaagaag gacgcgcagg ggagactgca gctctgcgcc gctgcctcga 6900 ctcggcctat agtcagaaag gcaggggtgc aacgtggaag aaaggtcaag gtaaaggtgg 6960 aaaacccatt tgctcggaat gccttacctc cgtggctcga gggggaacgc gaacaataga 7020 ggaaactggt gcagttagcg cgggcaactg ctaaagaatg ttcaagaatg cttctgtctc 7080 cacgcgattg tgaggacgtc tgagcgtttt tctgtgtttg cttctcgtgt ttcgcgggtg 7140 tgttgtgcca ttttcggtgg cagcgcacat gtatctgatc cccagaatac ggaaaagaag 7200 caatcacacg aagttttgta tcatccggcg gatttggcgt tagacctgac agcaacggcc 7260 ataggtttct cgcgtgtgtt cggcgcttcg atggcccaaa gagctcgcat ggtgccgcgt 7320 ttctatggat tcgtttacca ggaaatcgac gatttcaccg ccgaggattt tgaggtcgtg 7380 ggctacgtcc cgcacggacg aatccagatg gagatggctg tctagcggaa atacagaagc 7440 tgcccgtctc tcgttttcct ctcttttcgg agggatcagg gagagtgcct cgggtcggag 7500 agagctgacg agggggtgcc agagacccct gtgtccttta tcgaagaaaa gggatgactc 7560 ttcatgtggc atttcacaca gtctcacctc gccttgtttt ctttttgtca atcagaacga 7620 aagcgagttg cgggtgacgc agatgtgcgt gtatccactc gtgaatgcgt tatcgttctg 7680 tatgccgcta gagtgctgga ctgttgctgt ctgcccacga cagcagacaa ctttccttct 7740 atgcacttgc aggatggtgc agcgcaaacg acggagagaa aggagcaccc tctcagtttc 7800 cctacgatgt gctgtcagtt tcgactcttc accgcgaacg attggcgata cgtctctgtt 7860 gacttgttag gctccgacca cgaagctccc ttaactaaat aagccgcgac acctaagtgt 7920 acaccatttg cagatcgata atctgcgacc gctgaatccg tccagatcag taaaaccgca 7980 ccacctaagt gtaaaccttg tttaggtcga taaaatgcta ccaaccccca cccacaatcg 8040 agccttgagc gtttctgcgc acgcgttggc ctacgtgact tgctgatgcc tgcctctggc 8100 cattcatgcc agtcagtgcg cataaaaatg tggacacagt cggttgacaa gtgttctggc 8160 aggctacagt gacacgggga aatcgtttct gagagagcac caaaaccttt tagctgtcac 8220 catcttcttc gcttgctacg ttgtctgatt tgtcgttttt cctctcggtg tgaagcgttt 8280 ttcgtaacgt caatcgaccc aggacactgc ttccagctgt ctgtgccgtc ctgtcgtgac 8340 cagtttgtcg ccgcgacttt atgagctttt tcctgttgag aaagcggttc ggcggccgc 8399 // |
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