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EBI DbfetchID L07642; SV 1; linear; genomic DNA; STD; PRO; 1900 BP. XX AC L07642; XX DT 10-DEC-1992 (Rel. 34, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Bacillus stearothermophilus DNA modification methyltransferase (VIM) gene, DE complete cds. XX KW methyltransferase; restriction-modification system. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-1900 RX DOI; 10.1016/0378-1119(91)90543-K RX PUBMED; 1864512. RA Vasquez C., Saavedra C., Gonzalez E.; RT "Cloning the BstVI restriction-modification system in Escherichia coli"; RL Gene 102(1):83-85(1991). XX RN [2] RP 1-1900 RX DOI; 10.1016/0378-1119(93)90676-T RX PUBMED; 8370531. RA Gonzalez E., Vasquez C.; RT "Characterization of the bstVIRM genes encoding the Bacillus RT stearothermophilus V restriction-modification system"; RL Gene 131(1):103-106(1993). XX FH Key Location/Qualifiers FH FT source 1..1900 FT /organism="Geobacillus stearothermophilus" FT /strain="V" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT -35_signal 113..118 FT -10_signal 141..147 FT RBS 191..193 FT /note="putative" FT CDS 202..1887 FT /codon_start=1 FT /transl_table=11 FT /gene="VIM" FT /standard_name="BstVI methylase" FT /product="DNA modification methyltransferase" FT /function="methylates the adenine residue in the sequence FT 5'-CTCGAG-3'" FT /db_xref="GOA:P43422" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="UniProtKB/Swiss-Prot:P43422" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA51408.1" FT /translation="MVTQHSYRMHGAVYTKPIIVDLILDLTGYTSDKNLENFKLLDPSF FT GDGVFLEAAVHRLMDSLIRRGYRPNELIDHLGNCIRGIELRLEAYQAGRHRLQKVLEGY FT GFSKPEINWLINQWIIQADFLLWQEDTTEAIKFDFVVGNPPYVRQELIQDELIKKYRKR FT YTTIYDRADLYVPFIQHSLELLSEQGTLGIICSDRFTKNRYGKKLRKFITDNYKVRYIV FT DLHKTSPFENEVTAYPAIYVIKTKNYDKSVVRAVYTEVITSKVCQDAKDFLLSNQKPDQ FT SSKEMKTYVFSEWFAGDEPWIIQSQECREILRRLENRFPLIEDDVHSCKIRIGVATGAD FT KVYIVDPQQVDIEPEVLLPLVTTADISSGRIIWSGKHVINPFNSDGGLINLDDFPRLKT FT YFQQHEEIIKNRNVAKKNPSQWFRTIDRIYPEIVHQPKLLIPDMKNTNHIVKDDGAFYP FT HHNLYYILPGNWNIDILRAILLSSVVKFFIWSYATKMRGDTLRYQAQYLRKIRLPDPKS FT LTNDQKERLMDERVIQSQEYLDSIVAEIYQLSKTEIEIIKDALE" XX SQ Sequence 1900 BP; 621 A; 335 C; 380 G; 564 T; 0 other; aagcttccat acattcaaac gggcagttca taaccttatc ttttctctgc ttttcattaa 60 gagcagagtg tatgaccata acataattat cgctatccat tacgtagtat atgtgccagg 120 gggaagggaa cttaaaacca tatttaagaa gaaatatgtt attcttagga atagacctat 180 atgtagtggg ggtaactcta tatggtaaca caacatagtt atagaatgca tggagcggtc 240 tacaccaaac ccataattgt tgaccttata ctagatttaa cagggtatac ttcggacaaa 300 aatcttgaga actttaaact cttagacccg tcatttggag atggagtatt cctagaagca 360 gccgtccatc gtctgatgga ctcgttgata agacgtggat atcgtcctaa cgaattaata 420 gatcacttag gtaattgtat tcgagggata gagttaaggt tagaagccta ccaagctggt 480 cgacatcggt tacaaaaagt gttagaggga tatggttttt ccaagccgga gattaactgg 540 ttaatcaatc aatggatcat ccaagctgat tttttacttt ggcaagaaga tactactgaa 600 gcaataaaat ttgatttcgt ggttgggaac ccgccttacg ttaggcaaga attgattcaa 660 gatgagctga tcaaaaaata cagaaagcga tacacaacca tttatgatcg agcggattta 720 tatgtcccat tcattcagca ctctcttgaa ctacttagtg agcaaggtac tttagggatt 780 atttgttctg accgatttac caaaaatcga tacgggaaga agttgcgtaa atttataacc 840 gacaactata aagttcgtta tattgttgac ctacataaaa cttctccgtt tgaaaatgag 900 gttacagcct atccagcaat ctatgtaatc aagactaaga actatgacaa atctgtggtg 960 cgggcagttt acaccgaagt tattacttca aaggtctgtc aagacgcaaa agatttttta 1020 ttatctaatc aaaagccaga tcaatcttcg aaagaaatga aaacctatgt tttctctgaa 1080 tggtttgccg gagatgagcc gtggattatt caatctcaag agtgtcgtga gatcctaaga 1140 aggcttgaga accgatttcc gctgatcgaa gatgatgtac acagttgcaa aatcaggatc 1200 ggtgtagcta ccggcgctga taaagtttat attgttgatc ctcaacaggt cgatattgaa 1260 ccggaggtat tgctgccctt agtaacaact gcagatatat ctagcggtcg gatcatttgg 1320 tctggaaagc atgttattaa cccatttaat tctgacggtg gactaattaa tttagatgat 1380 tttccacgtc ttaagacgta ttttcaacaa catgaagaaa ttattaagaa ccgtaacgta 1440 gcaaagaaaa atccttcgca gtggtttaga acaattgatc gtatataccc ggaaattgtt 1500 catcaaccga agttacttat tcctgatatg aaaaacacta accatatcgt taaggacgat 1560 ggggcttttt atcctcatca caatttatac tatatccttc cgggaaattg gaatattgac 1620 atcctacggg ctattctatt gtcctcggtt gttaaatttt ttatatggtc atacgcgaca 1680 aaaatgagag gagataccct tcgttatcaa gctcaatatt tgcgaaaaat ccgtcttcct 1740 gatccgaaat cattaacaaa tgatcagaaa gaacgattaa tggacgaaag agtcattcaa 1800 tctcaagagt acttggatag tattgtagcc gagatttatc aactatcgaa gactgaaatc 1860 gagattatta aagatgcatt ggaataggag gatattaaag 1900 // |
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