EBI Dbfetch

ID   L06214; SV 1; linear; genomic DNA; STD; PRO; 978 BP.
XX
AC   L06214;
XX
DT   05-MAR-1993 (Rel. 35, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 10)
XX
DE   Streptomyces clavuligerus clavaminate synthase 2 (cs2) gene, complete cds.
XX
KW   2-oxo-glutarate-dependent oxygenase;
KW   alpha-ketoglutarate-dependent oxygenase; beta-lactam antibiotics;
KW   clavaminate synthase 2; clavulanic acid biosynthesis;
KW   oxidative cyclization.
XX
OS   Streptomyces clavuligerus
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-978
RX   DOI; 10.1021/bi00165a015
RX   PUBMED; 1472501.
RA   Marsh E.N., Chang M.D.-T., Townsend C.A.;
RT   "Two isozymes of clavaminate synthase central to clavulanic acid formation:
RT   cloning and sequencing of both genes from Streptomyces clavuligerus";
RL   Biochemistry 31(50):12648-12657(1992).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..978
FT                   /organism="Streptomyces clavuligerus"
FT                   /mol_type="genomic DNA"
FT                   /cell_line="XL1 Blue"
FT                   /tissue_lib="ATCC 278"
FT                   /db_xref="taxon:1901"
FT   CDS             1..978
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="cs2"
FT                   /product="clavaminate synthase 2"
FT                   /db_xref="GOA:Q05582"
FT                   /db_xref="HSSP:1DRY"
FT                   /db_xref="InterPro:IPR003819"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q05582"
FT                   /protein_id="AAA26723.1"
FT                   /translation="MASPIVDCTPYRDELLALASELPEVPRADLHGFLDEAKTLAARLP
FT                   EGLAAALDTFNAVGSEDGYLLLRGLPVDDSELPETPTSTPAPLDRKRLVMEAMRALAGR
FT                   RLGLHTGYQELRSGTVYHDVYPSPGAHYLSSETSETLLEFHTEMAYHILQPNYVMLACS
FT                   RADHENRAETLVGSVRKALPLLDEKTRARLFDRKVPCCVDVAFRGGVDDPGAIANVKPL
FT                   YGDANDPFLGYDRELLAPEDPADKEAVAHLSQALDDVTVGVKLVPGDVLIIDNFRTTHA
FT                   RTPFSPRWDGKDRWLHRVYIRTDRNGELSGGERAGDTISFSPRR"
XX
SQ   Sequence 978 BP; 143 A; 371 C; 324 G; 140 T; 0 other;
     atggcctctc cgatagttga ctgcaccccg taccgcgacg agctgctcgc gctcgcctcc        60
     gagcttcccg aggtgccgcg cgcggacctc catggcttcc tcgacgaggc gaagacgctg       120
     gccgcccgtc tcccggaggg gctggccgcc gctctcgaca ccttcaacgc cgtgggcagc       180
     gaggacggtt atctgctgct gcgcgggctg cccgtcgacg acagcgagct gcccgagacg       240
     ccgacctcca ccccggcccc gctggaccgc aagcggctgg tgatggaggc catgcgcgcg       300
     ctggccggcc gccggctcgg tctgcacacg gggtaccagg agctgcgctc gggcacggtc       360
     taccacgacg tgtacccgtc gcccggcgcg cactacctgt cctcggagac ctccgagacg       420
     ctgctggagt tccacacgga gatggcgtac cacatcctcc agccgaacta cgtcatgctg       480
     gcctgctccc gcgcggacca cgagaaccgg gcggagacgc tggtcggctc ggtccgcaag       540
     gcgctgcccc tgctggacga gaagacccgg gcccgtctct tcgaccgcaa ggtgccctgc       600
     tgcgtggacg tggccttccg cggcggggtc gacgacccgg gcgcgatcgc caacgtcaag       660
     ccgctctacg gggacgcgaa cgacccgttc ctcgggtacg accgcgagct gctggcgccg       720
     gaggaccccg cggacaagga ggccgtcgcc catctgtccc aggcgctcga cgatgtgacc       780
     gtcggggtga agctcgtccc cggtgacgtc ctcatcatcg acaacttccg caccacgcac       840
     gcgcggacgc cgttctcgcc ccgctgggac gggaaggacc gctggctgca ccgcgtctac       900
     atccgcaccg accgcaatgg agagctctcc ggcggcgagc gcgcgggcga caccatctcg       960
     ttctcgccgc gccgctga                                                     978
//