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EBI DbfetchID L06134; SV 1; linear; genomic DNA; STD; PRO; 3955 BP. XX AC L06134; XX DT 07-JAN-1993 (Rel. 34, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Microbispora bispora glucan-glucohydrolase (bglA) gene, complete cds. XX KW aryl-beta-D-glucosidase; beta-glucosidase A; cellodextrinase. XX OS Thermobispora bispora OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Pseudonocardineae; Pseudonocardiaceae; Thermobispora. XX RN [1] RP 1-3955 RX DOI; 10.1016/0378-1119(96)00076-5 RX PUBMED; 8654999. RA Goyal A.K., Eveleigh D.E.; RT "Cloning, sequencing and analysis of the ggh-A gene encoding a RT 1,4-beta-D-glucan glucohydrolase from Microbispora bispora"; RL Gene 172(1):93-98(1996). XX FH Key Location/Qualifiers FH FT source 1..3955 FT /organism="Thermobispora bispora" FT /mol_type="genomic DNA" FT /tissue_lib="NRRL 15568" FT /db_xref="taxon:2006" FT RBS 345..349 FT /gene="bglA" FT /note="based on similarity; putative" FT CDS 354..3314 FT /codon_start=1 FT /transl_table=11 FT /gene="bglA" FT /product="glucan-glucohydrolase" FT /function="hydrolyses beta-1,4-glucosidic bonds e.g. in FT cellotriose, cellotetraose, cellopentose and cellohexose" FT /EC_number="3.2.1.74" FT /note="similar to family 3 of glycosyl-hydrolases (contains FT fungal & bacterial beta-glucosidases)" FT /db_xref="GOA:Q59506" FT /db_xref="HSSP:1NAE" FT /db_xref="InterPro:IPR001764" FT /db_xref="InterPro:IPR002772" FT /db_xref="InterPro:IPR005084" FT /db_xref="InterPro:IPR006584" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR008999" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q59506" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB36835.1" FT /translation="MPLPRPLRAMPLRRPLRRAPRVTLVLALTAAPARADADLPFRDPT FT LPLAQRIDDLLGRLTLDEKISLLHQYQPPIERLGIKSFKTGTEALHGVAWSTDVTDNGA FT VVTANGTVFPQAVGLASTWDPELNQRVGTVGEEARGFHAQNPVVWGLNLWAPVVNLLRD FT PRWGRNEEGYSEDPLLTGAIAIAYGSGIQGDDPDHLRAAPTLKHYLANNNEIRRDTTSS FT NLPPRVKHEYYEAPFRAAITAGAATGVMTAYNLVNGRPATVNPDLNDTVRTWTDRDLLN FT VTDAGAPNNLVGSQAYFATLAEADAAALKAGIDSFTTDETNSAPTITAIKTALSQGLLT FT EQDIDTAVRHILGIRFRLGEFDPDGGPYAKITPDVIDSPAHRRLARETAAKAMVLLKNE FT RGTLPLDPGKKVAVVGPLADVLYTDWYSGRPTYQVTPLDGIRERAASVTSSEGVDRVAF FT KDLATGQVHHRGERPEGADLRLSATTIGETEQFDVFDWGQGIVTLRSVANGKYVSRANW FT STLVNNADQPSGWFVQEQFKLEEQDDGSYLIRYAGYETAYDWFGPNTYVKAAPDGTLTL FT TTAGDATRFAKEVVRSGIDDAVAKAKEADVAVVVVGSMPFINGREDHDRTDMNLAEGQE FT ALVKAVFNANPRTVVVLENSYPTTINWIDEHVPAILWTTHAGAETGNALADVLYGDVNP FT AGRLTQTWYLGGRLPDILDYDIVQRDRTYLYFKGTPLYPFGHGLSYTTFRYQGLRVAEK FT GDAYEVSVRVTNTGHRAGDEVVQVYTHQRTSRVKQPVKQLRAFQRITLAPGQSKTVTFT FT IRKADLALWDVTRNKWTVETSAHDVMVGASSADIRQRATIHVKGERIPDRDLSVPTRAI FT DFDGYSGVELVDETKARGDAVAGSTGDWIVFKDVDLKRRPSRVTAGVASTSGGSIELRL FT GSPKGKLIATVPVAATGDVYRYETAAARVTGPSGVKDLYLVFQGDVRIKDLSLTSG" XX SQ Sequence 3955 BP; 640 A; 1472 C; 1335 G; 508 T; 0 other; ggcgcggcgc tcgacggcgc ggggaggtgg cgggcgctgt ggcggatcgt cgtgccgatc 60 agccggccca ccctcaccgt gctgctcatc ttcttcttca tctggacctg gaacgagttc 120 ttcctgccgc tgatctttct gatctccaac gagaaccaga ccgtgccggt cgtgctcggc 180 gtcctgcaag gggagaagat gatggacgcc accatgtcag cctcgcgctg ctcggcatcc 240 tcccggccgt cgttcttctt tcctgatctt ccagcgcacg ctgacccgcg gcatcacggt 300 cggtgcgatc aagtgagccc gcacgcgtcc ctcacgcctg acctggaggc gcaatgccgt 360 tacctaggcc cctccgcgcg atgccgttac gtaggcccct ccggcgcgct ccccgcgtca 420 cgctcgtcct cgccctgacg gccgcccccg cgcgagccga cgccgacctt cccttccgcg 480 accccacgct cccgctcgcc cagcggatcg acgacctgct cggcaggctg acgctcgacg 540 agaagatctc cctcctccac cagtaccagc cgccgatcga gcgcctcggc atcaagagct 600 tcaagaccgg caccgaggcg ctgcacgggg tcgcctggtc caccgacgtc accgacaacg 660 gcgccgtcgt cacggcgaac ggcaccgtgt tcccccaggc ggtcggcctg gcgagcacct 720 gggacccgga gctgaatcag cgggtcggca ccgtcggcga ggaggcgcgc ggattccacg 780 cgcagaaccc ggtggtctgg gggctcaacc tgtgggcccc ggtcgtcaac ctgctgcgcg 840 acccgcgctg ggggcgcaac gaggaggggt actccgagga ccccctgctc accggcgcga 900 tcgccatcgc gtacggctcg ggcatccagg gggacgaccc cgaccacctc agggcggccc 960 ccacgctcaa gcactacctc gccaacaaca acgagatccg ccgcgacacc acctcgtcga 1020 acctgccgcc ccgggtcaag cacgagtact acgaggcgcc gttccgggcg gcgatcacgg 1080 ccggcgcggc caccggtgtc atgaccgcgt acaacctggt gaacggccgg ccggccacgg 1140 tcaaccccga cctcaacgac accgtccgga cctggaccga ccgggacctg ctcaacgtca 1200 ccgacgccgg ggcgcccaac aacctcgtcg gctcgcaggc ctacttcgcc accctggccg 1260 aggccgacgc cgccgcgctc aaggcgggga tcgacagctt caccacggac gagacgaaca 1320 gcgcccccac gatcaccgcg atcaagacgg cgctgtcgca gggcctgctc accgaacagg 1380 acatcgacac cgccgtccgg cacatcctcg gcatccgctt ccggctgggc gagttcgacc 1440 cggacggcgg gccgtacgcg aagatcaccc cggatgtgat cgacagcccg gcccaccgga 1500 ggctcgcccg cgagaccgcg gccaaggcca tggtgctgct gaagaacgag cgcggcaccc 1560 tgccgctcga ccccgggaag aaggtcgcgg tggtcgggcc gctcgccgac gtgctctaca 1620 ccgactggta ctcgggcagg ccgacgtacc aggtcacccc gctcgacggc atccgggagc 1680 gggcggcctc ggtgacctcc tcggagggcg tcgaccgggt cgcgttcaag gacctcgcca 1740 ccgggcaggt acatcaccgc ggggagcgcc ccgagggcgc cgacctcagg ctgagcgcca 1800 ccacgatcgg cgagaccgag cagttcgacg tgttcgactg gggccagggc atcgtgacgc 1860 tgcgcagcgt cgccaacggc aagtacgtca gccgggccaa ctggtcgacg ctggtcaaca 1920 acgccgacca gccgagcggc tggttcgtgc aggagcagtt caagctggag gagcaggacg 1980 acggctccta cctgatcagg tacgccgggt acgagaccgc ctacgactgg ttcggcccga 2040 acacctacgt caaggccgcc cccgacggca cgctcaccct caccacggcc ggcgacgcca 2100 cgcggttcgc caaggaggtg gtccgcagcg gcatcgacga cgcggtcgcc aaggcgaagg 2160 aggccgacgt ggcggtggtc gtcgtcggca gcatgccgtt catcaacggc cgcgaggacc 2220 acgaccgcac cgacatgaac ctggccgagg gccaggaggc gctggtcaag gcggtgttca 2280 acgccaaccc ccgcaccgtc gtcgtgctgg agaacagcta cccgacgacg atcaactgga 2340 tcgacgagca cgtgccggcc atcctctgga ccacccacgc cggggcggag accgggaacg 2400 cgctcgccga cgtgctctac ggcgacgtca acccggccgg gcggctcacc cagacctggt 2460 acctcggcgg acgactgccg gacatcctcg actacgacat cgtgcagcgg gaccggacct 2520 acctgtactt caagggcacc ccgctctacc ccttcgggca cggcctgtcg tacacgacct 2580 tccgctacca gggcctgcgg gtggccgaga agggcgacgc ctacgaggtc tcggtacggg 2640 tgaccaacac cggccaccgg gcgggcgacg aggtcgtcca ggtctacacc caccagcgga 2700 cctcgcgggt gaagcagccg gtcaagcagc tccgcgcctt ccagcggatc acgctcgccc 2760 cgggccagag caagaccgtg accttcacca tccgcaaggc cgacctggcg ctgtgggacg 2820 tgacccggaa caagtggacg gtcgagacct ccgcccacga cgtcatggtc ggggcgtcct 2880 cggccgacat ccggcagcgg gccacgatcc acgtcaaggg tgagcggatc cccgaccggg 2940 acctgagcgt ccccacccgg gcgatcgact tcgacggcta ttcgggcgtc gagctggtgg 3000 acgagaccaa ggcgcgcggg gacgccgtgg cgggctccac cggtgactgg atcgtcttca 3060 aggacgtcga cctcaagcgg cggccgtccc gggtgaccgc cggggtcgcc tccacctccg 3120 gcggctccat cgagctccgc ctcggctcgc ccaagggcaa gctgatcgcc accgtgcccg 3180 tcgccgccac cggcgacgtc taccggtacg agacggcggc cgcccgggtc accgggccgt 3240 ccggggtcaa ggacctctac ctggtgttcc agggcgacgt gcggatcaag gacctgagcc 3300 tcacctcggg gtgaccggac gccgcgccgt ccgtacggcc acgccggacc gccggccggg 3360 ccgcccacca cgcgggcccg tccggaggtc cggcaccggc cgggaggagc ggtccccggt 3420 gcggccgcgg ggaacgcgcg cgtccgccgg ggccatcgaa gagaagacaa gccgtccggc 3480 ggccgccgca gggggaggag gagcggcgat ctccgggggg cgcgcaccac gtgcggggac 3540 gtgcgcgaac gcgggtgatc cagggctgtt tcgctattct ccccgatatg gccgaatcgg 3600 tgagccagac gctggctcgc ggtatcagct ggctgcgccc gctcggcacc ggcggtatgg 3660 gcacggtgtg gctggccagg gacacggtgc tcgaccgcga ggctggtcaa ggagctgcgc 3720 ctcaccgacg gcctctccga ggcggaacgg ccgagctgat ggccagggtc atgcgggagg 3780 cggaggtgac cgcccgcctc cggcaccccg gcatcgtggc cctgcacgac gtctgatcga 3840 gggcggccgg ccgtggctcg tcatggagct cctccacggc cgggacctcg cccgggagat 3900 cgcggccaac ggccgatgcc gccccacgtg gtggccggca tcggcgcccg ggtgc 3955 // |
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